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  • 98
    New England Biolabs rnase inhibitor human placenta
    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the <t>RNase</t> inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) <t>RT-qPCR</t> detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.
    Rnase Inhibitor Human Placenta, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase inhibitor human placenta/product/New England Biolabs
    Average 98 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor human placenta - by Bioz Stars, 2020-07
    98/100 stars
      Buy from Supplier

    99
    New England Biolabs human placenta rnase inhibitor
    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the <t>RNase</t> inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) <t>RT-qPCR</t> detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.
    Human Placenta Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human placenta rnase inhibitor/product/New England Biolabs
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    human placenta rnase inhibitor - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Journal: bioRxiv

    Article Title: Comparison of SARS-CoV-2 Indirect and Direct Detection Methods

    doi: 10.1101/2020.05.12.092387

    Figure Lengend Snippet: One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Article Snippet: With direct RT-qPCR we found that simply adding RNase inhibitor greatly improved sensitivity, without need for any other treatments (e.g. lysis buffers or boiling).

    Techniques: RNA Extraction, Lysis, Quantitative RT-PCR, SYBR Green Assay