human pla 2 r wild type plasmid Search Results


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    OriGene human pla2r plasmid
    Correlation analysis results
    Human Pla2r Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pla2r plasmid/product/OriGene
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human pla2r plasmid - by Bioz Stars, 2023-12
    80/100 stars
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    PLA2R1 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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    Correlation analysis results

    Journal: Scientific Reports

    Article Title: sPLA2 IB induces human podocyte apoptosis via the M-type phospholipase A2 receptor

    doi: 10.1038/srep06660

    Figure Lengend Snippet: Correlation analysis results

    Article Snippet: The human PLA2R plasmid and empty vectors were purchased from Origene Company (USA).

    Techniques:

    A. Immunofluorescence staining of mouse podocyte and human podocyte PLA2R expression (original magnification, ×400). a Negative control group: PBS instead of the PLA2R antibody. b Mouse podocytes. c Human podocytes. B. Representative Western blotting of the levels of PLA2R in mouse podocytes and in human podocytes. * p < 0.05 vs. mouse podocytes.

    Journal: Scientific Reports

    Article Title: sPLA2 IB induces human podocyte apoptosis via the M-type phospholipase A2 receptor

    doi: 10.1038/srep06660

    Figure Lengend Snippet: A. Immunofluorescence staining of mouse podocyte and human podocyte PLA2R expression (original magnification, ×400). a Negative control group: PBS instead of the PLA2R antibody. b Mouse podocytes. c Human podocytes. B. Representative Western blotting of the levels of PLA2R in mouse podocytes and in human podocytes. * p < 0.05 vs. mouse podocytes.

    Article Snippet: The human PLA2R plasmid and empty vectors were purchased from Origene Company (USA).

    Techniques: Immunofluorescence, Staining, Expressing, Negative Control, Western Blot

    A. Representative Western blotting results for cPLA2α, ERK1/2 and their phosphorylation and for the PLA2R at different time points (n = 3). All of the groups were treated with a 10 −6 M concentration of sPLA2 IB. B. Representative Hoechst 33342 staining of apoptotic podocytes stimulated by 10 −6 M sPLA2 IB at various time points (original magnification, ×400). C. HPLC of podocyte AA production at different time points (n = 3). All of the groups were treated with a 10 −6 M concentration of sPLA2 IB. D. Flow cytometry analysis of apoptosis in differentiated podocytes at different time points (n = 3). All of the groups were treated with a 10 −6 M concentration of sPLA2 IB. * p < 0.05 vs. 0 h group, # p < 0.01 vs. 0 h group.

    Journal: Scientific Reports

    Article Title: sPLA2 IB induces human podocyte apoptosis via the M-type phospholipase A2 receptor

    doi: 10.1038/srep06660

    Figure Lengend Snippet: A. Representative Western blotting results for cPLA2α, ERK1/2 and their phosphorylation and for the PLA2R at different time points (n = 3). All of the groups were treated with a 10 −6 M concentration of sPLA2 IB. B. Representative Hoechst 33342 staining of apoptotic podocytes stimulated by 10 −6 M sPLA2 IB at various time points (original magnification, ×400). C. HPLC of podocyte AA production at different time points (n = 3). All of the groups were treated with a 10 −6 M concentration of sPLA2 IB. D. Flow cytometry analysis of apoptosis in differentiated podocytes at different time points (n = 3). All of the groups were treated with a 10 −6 M concentration of sPLA2 IB. * p < 0.05 vs. 0 h group, # p < 0.01 vs. 0 h group.

    Article Snippet: The human PLA2R plasmid and empty vectors were purchased from Origene Company (USA).

    Techniques: Western Blot, Concentration Assay, Staining, Flow Cytometry

    A. Representative Western blotting results for cPLA2α, ERK1/2 and their phosphorylation and for the PLA2R at different concentrations of sPLA2 IB (n = 3). All of the groups were treated with sPLA2 IB for 2 h. B. Representative Hoechst 33342 staining of apoptotic podocytes stimulated by different concentrations of sPLA2 IB for 2 h (original magnification, ×400). C. HPLC of podocyte AA production at different concentrations of sPLA2 IB (n = 3). All of the groups were treated with sPLA2 IB for 2 h. D. Flow cytometry analysis of apoptosis in differentiated podocytes stimulated by different concentrations of sPLA2 IB (n = 3). All of the groups were treated with sPLA2 IB for 2 h. * p < 0.05 vs. 0 M group, # p < 0.01 vs. 0 M group.

    Journal: Scientific Reports

    Article Title: sPLA2 IB induces human podocyte apoptosis via the M-type phospholipase A2 receptor

    doi: 10.1038/srep06660

    Figure Lengend Snippet: A. Representative Western blotting results for cPLA2α, ERK1/2 and their phosphorylation and for the PLA2R at different concentrations of sPLA2 IB (n = 3). All of the groups were treated with sPLA2 IB for 2 h. B. Representative Hoechst 33342 staining of apoptotic podocytes stimulated by different concentrations of sPLA2 IB for 2 h (original magnification, ×400). C. HPLC of podocyte AA production at different concentrations of sPLA2 IB (n = 3). All of the groups were treated with sPLA2 IB for 2 h. D. Flow cytometry analysis of apoptosis in differentiated podocytes stimulated by different concentrations of sPLA2 IB (n = 3). All of the groups were treated with sPLA2 IB for 2 h. * p < 0.05 vs. 0 M group, # p < 0.01 vs. 0 M group.

    Article Snippet: The human PLA2R plasmid and empty vectors were purchased from Origene Company (USA).

    Techniques: Western Blot, Staining, Flow Cytometry

    A. Western blotting analysis of cPLA2α, ERK and their phosphorylation and for the PLA2R in cultured podocytes stimulated by 10 −6 M sPLA2 IB for 2 h and treated with no plasmid, scrambled plasmid, PLA2R plasmid, scrambled siRNA or PLA2R siRNA (n = 3). B. Representative immunofluorescence staining of PLA2R and sPLA2 IB expression in cultured podocytes stimulated by 10 −6 M sPLA2 IB for 2 h and treated with no plasmid, PLA2R plasmid or PLA2R siRNA (original magnification, ×400). C. HPLC of podocyte AA production in cultured podocytes stimulated with 10 −6 M sPLA2 IB for 2 h and treated with no plasmid, scrambled plasmid, PLA2R plasmid, scrambled siRNA or PLA2R siRNA (n = 3). D. Flow cytometry analysis of apoptosis in differentiated podocytes stimulated by 10 −6 M sPLA2 IB for 2 h and treated with no plasmid, scrambled plasmid, PLA2R plasmid, scrambled siRNA or PLA2R siRNA (n = 3). * p < 0.05 vs. control group, # p < 0.05 vs. scrambled plasmid group or scrambled siRNA group.

    Journal: Scientific Reports

    Article Title: sPLA2 IB induces human podocyte apoptosis via the M-type phospholipase A2 receptor

    doi: 10.1038/srep06660

    Figure Lengend Snippet: A. Western blotting analysis of cPLA2α, ERK and their phosphorylation and for the PLA2R in cultured podocytes stimulated by 10 −6 M sPLA2 IB for 2 h and treated with no plasmid, scrambled plasmid, PLA2R plasmid, scrambled siRNA or PLA2R siRNA (n = 3). B. Representative immunofluorescence staining of PLA2R and sPLA2 IB expression in cultured podocytes stimulated by 10 −6 M sPLA2 IB for 2 h and treated with no plasmid, PLA2R plasmid or PLA2R siRNA (original magnification, ×400). C. HPLC of podocyte AA production in cultured podocytes stimulated with 10 −6 M sPLA2 IB for 2 h and treated with no plasmid, scrambled plasmid, PLA2R plasmid, scrambled siRNA or PLA2R siRNA (n = 3). D. Flow cytometry analysis of apoptosis in differentiated podocytes stimulated by 10 −6 M sPLA2 IB for 2 h and treated with no plasmid, scrambled plasmid, PLA2R plasmid, scrambled siRNA or PLA2R siRNA (n = 3). * p < 0.05 vs. control group, # p < 0.05 vs. scrambled plasmid group or scrambled siRNA group.

    Article Snippet: The human PLA2R plasmid and empty vectors were purchased from Origene Company (USA).

    Techniques: Western Blot, Cell Culture, Plasmid Preparation, Immunofluorescence, Staining, Expressing, Flow Cytometry