human peripheral blood mononuclear cells pbmcs Search Results


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  • 96
    Lonza human pbmc human peripheral blood mononuclear cells cryopreserved
    Human Pbmc Human Peripheral Blood Mononuclear Cells Cryopreserved, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human peripheral blood mononuclear cells pbmcs
    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells <t>(PBMCs)</t> and <t>K562</t> cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AllCells LLC human peripheral blood mononuclear cells pbmc
    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells <t>(PBMCs)</t> and <t>K562</t> cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p
    Human Peripheral Blood Mononuclear Cells Pbmc, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioreclamationIVT human peripheral blood mononuclear cells pbmc
    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells <t>(PBMCs)</t> and <t>K562</t> cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p
    Human Peripheral Blood Mononuclear Cells Pbmc, supplied by BioreclamationIVT, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ZenBio human peripheral blood mononuclear cells pbmcs
    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells <t>(PBMCs)</t> and <t>K562</t> cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by ZenBio, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems human peripheral blood mononuclear cells pbmcs
    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells <t>(PBMCs)</t> and <t>K562</t> cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc human peripheral blood mononuclear cells pbmcs
    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells <t>(PBMCs)</t> and <t>K562</t> cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 93/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Astarte Biologics human peripheral blood mononuclear cells pbmcs
    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells <t>(PBMCs)</t> and <t>K562</t> cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson human peripheral blood mononuclear cells pbmcs
    CD40L expressed by recombinant ALVAC virus enhances specific antiviral CTL activity. CD4 + T cell-containing or -depleted <t>PBMCs</t> from four HIV-1-infected individuals and three HIV-1-uninfected individuals were cocultured with autologous MDDCs that were either pulsed or not with HLA-restricted epitopes of HIV-1 proteins (for HIV-1 infected individuals) or EBV proteins (for HIV-1-uninfected individuals). MDDCs were previously infected or not (medium) with parental ALVAC II or ALVAC recombinant vA3131-2 expressing human CD40L at an MOI of 10 for 48 h. On day 10, specific CTL activity was assessed by intracellular flow cytometric analysis of IFN-γproducing CD8 + T cells and 51 Cr release assay. (A) Representative intracellular IFN-γ flow cytometric data obtained from HIV-1-positive participant #1. (B) Summary data from intracellular IFN-γ flow <t>cytometry</t> of HIV-1-positive participant #2–#4 are graphically depicted. Open bars represent CD4 + T cell-containing condition and dark bar represent CD4 + T cell -depleted conditions. (C) A representative 51 Cr release assay result from HIV-1-positive participant #1 is shown. Similar results were obtained with HIV-1-positive participant #2 (data not shown). (D) Summary data from intracellular IFN-γ flow cytometric analysis of EBV-positive participant #5–#7 are graphically depicted. Open bars represent CD4 + T cell -containing condition and dark bars represent CD4 + T cell -depleted conditions. (E) A representative 51 Cr release assay result from EBV-positive participant #6 is shown. Similar results were obtained with EBV-positive participant #5 (data not shown). DC, MDDCs not pulsed with peptide; DCp, MDDCs pulsed with peptide; vA3131-2/DCp, vA3131-2-infected MDDCs pulsed with peptide; ALVAC/DCp, parental ALVAC II-infected MDDCs pulsed with peptide.
    Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore human peripheral blood mononuclear cells pbmc
    Detection of ApoE isoforms in differentiating hNP1 cells. Differentiating hNP1 cells were exposed to medium alone (Unt), mock-infected <t>PBMC</t> supernatants (Mock), and <t>HIV-infected</t> PBMC supernatants (HIV), and to rApoE3 or rApoE4 at 3 μg/ml, as described in Materials and Methods. At day 16 of differentiation, and 24 h after replenishment of rApoE in the cultures, cells were lysed for quantitative immunoblotting to detect ApoE. Lower blot depicts representative endogenous ApoE detected in cell culture lysates with molecular weight of 34 kDa, and rApoE with molecular weight of 36 kDa. Upper blot corresponds to beta-actin used as an internal control. For comparison, recombinant ApoE3 and ApoE4 were loaded directly on the gel at a 3 ng per lane, as shown. Bar graphs present ApoE signal normalized to beta-actin; each bar represents the mean of two experiments
    Human Peripheral Blood Mononuclear Cells Pbmc, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellular Technology Ltd frozen human peripheral blood mononuclear cells pbmcs
    Detection of ApoE isoforms in differentiating hNP1 cells. Differentiating hNP1 cells were exposed to medium alone (Unt), mock-infected <t>PBMC</t> supernatants (Mock), and <t>HIV-infected</t> PBMC supernatants (HIV), and to rApoE3 or rApoE4 at 3 μg/ml, as described in Materials and Methods. At day 16 of differentiation, and 24 h after replenishment of rApoE in the cultures, cells were lysed for quantitative immunoblotting to detect ApoE. Lower blot depicts representative endogenous ApoE detected in cell culture lysates with molecular weight of 34 kDa, and rApoE with molecular weight of 36 kDa. Upper blot corresponds to beta-actin used as an internal control. For comparison, recombinant ApoE3 and ApoE4 were loaded directly on the gel at a 3 ng per lane, as shown. Bar graphs present ApoE signal normalized to beta-actin; each bar represents the mean of two experiments
    Frozen Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/frozen human peripheral blood mononuclear cells pbmcs/product/Cellular Technology Ltd
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    AllCells LLC frozen human peripheral blood mononuclear cells pbmcs
    Detection of ApoE isoforms in differentiating hNP1 cells. Differentiating hNP1 cells were exposed to medium alone (Unt), mock-infected <t>PBMC</t> supernatants (Mock), and <t>HIV-infected</t> PBMC supernatants (HIV), and to rApoE3 or rApoE4 at 3 μg/ml, as described in Materials and Methods. At day 16 of differentiation, and 24 h after replenishment of rApoE in the cultures, cells were lysed for quantitative immunoblotting to detect ApoE. Lower blot depicts representative endogenous ApoE detected in cell culture lysates with molecular weight of 34 kDa, and rApoE with molecular weight of 36 kDa. Upper blot corresponds to beta-actin used as an internal control. For comparison, recombinant ApoE3 and ApoE4 were loaded directly on the gel at a 3 ng per lane, as shown. Bar graphs present ApoE signal normalized to beta-actin; each bar represents the mean of two experiments
    Frozen Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/frozen human peripheral blood mononuclear cells pbmcs/product/AllCells LLC
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    Lonza poietics peripheral blood mononuclear cells pbmcs
    ShK-186 inhibits antineutrophil cytoplasmic antibodies targeting proteinase 3 (PR3-ANCA) production in vitro . (A) IgG production after peripheral blood mononuclear cell <t>(PBMC)</t> stimulation with CpG, B cell-activating factor (BAFF), and interleukin (IL)-21 from 5 healthy controls (HCs) (open circles) and 13 granulomatosis with polyangiitis <t>(GPA)</t> patients (gray circles) in the presence and absence of 1 nM ShK-186. (B) PR3-ANCA production after PBMC stimulation with CpG, BAFF, and IL-21 from 13 GPA patients in the presence and absence of 1 nM ShK-186 (* p
    Poietics Peripheral Blood Mononuclear Cells Pbmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biochrom pbmcs human peripheral blood mononuclear cells pbmcs
    ShK-186 inhibits antineutrophil cytoplasmic antibodies targeting proteinase 3 (PR3-ANCA) production in vitro . (A) IgG production after peripheral blood mononuclear cell <t>(PBMC)</t> stimulation with CpG, B cell-activating factor (BAFF), and interleukin (IL)-21 from 5 healthy controls (HCs) (open circles) and 13 granulomatosis with polyangiitis <t>(GPA)</t> patients (gray circles) in the presence and absence of 1 nM ShK-186. (B) PR3-ANCA production after PBMC stimulation with CpG, BAFF, and IL-21 from 13 GPA patients in the presence and absence of 1 nM ShK-186 (* p
    Pbmcs Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare human peripheral blood mononuclear cells pbmcs pbmcs
    ShK-186 inhibits antineutrophil cytoplasmic antibodies targeting proteinase 3 (PR3-ANCA) production in vitro . (A) IgG production after peripheral blood mononuclear cell <t>(PBMC)</t> stimulation with CpG, B cell-activating factor (BAFF), and interleukin (IL)-21 from 5 healthy controls (HCs) (open circles) and 13 granulomatosis with polyangiitis <t>(GPA)</t> patients (gray circles) in the presence and absence of 1 nM ShK-186. (B) PR3-ANCA production after PBMC stimulation with CpG, BAFF, and IL-21 from 13 GPA patients in the presence and absence of 1 nM ShK-186 (* p
    Human Peripheral Blood Mononuclear Cells Pbmcs Pbmcs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AllCells LLC adcc assay human peripheral blood mononuclear cells pbmc
    <t>ADCC</t> of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of <t>PBMC</t> effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.
    Adcc Assay Human Peripheral Blood Mononuclear Cells Pbmc, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells (PBMCs) and K562 cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p

    Journal: Turkish Journal of Hematology

    Article Title: Tendency of K562 Chronic Myeloid Leukemia Cells Towards Cell Reprogramming

    doi: 10.4274/tjh.2018.0106

    Figure Lengend Snippet: Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells (PBMCs) and K562 cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p

    Article Snippet: Cell Culture Human CML cell line K562 and human peripheral blood mononuclear cells (PBMCs) were obtained from ATCC and Lonza, respectively.

    Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction

    Gene expression of reprogramming factors and pluripotency markers. RNA was isolated from peripheral blood mononuclear cells (PBMCs) and K562 cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for A) reprogramming factors and B) pluripotency markers. GAPDH was used as a reference gene and data were normalized to PBMCs. *p

    Journal: Turkish Journal of Hematology

    Article Title: Tendency of K562 Chronic Myeloid Leukemia Cells Towards Cell Reprogramming

    doi: 10.4274/tjh.2018.0106

    Figure Lengend Snippet: Gene expression of reprogramming factors and pluripotency markers. RNA was isolated from peripheral blood mononuclear cells (PBMCs) and K562 cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for A) reprogramming factors and B) pluripotency markers. GAPDH was used as a reference gene and data were normalized to PBMCs. *p

    Article Snippet: Cell Culture Human CML cell line K562 and human peripheral blood mononuclear cells (PBMCs) were obtained from ATCC and Lonza, respectively.

    Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction

    CD40L expressed by recombinant ALVAC virus enhances specific antiviral CTL activity. CD4 + T cell-containing or -depleted PBMCs from four HIV-1-infected individuals and three HIV-1-uninfected individuals were cocultured with autologous MDDCs that were either pulsed or not with HLA-restricted epitopes of HIV-1 proteins (for HIV-1 infected individuals) or EBV proteins (for HIV-1-uninfected individuals). MDDCs were previously infected or not (medium) with parental ALVAC II or ALVAC recombinant vA3131-2 expressing human CD40L at an MOI of 10 for 48 h. On day 10, specific CTL activity was assessed by intracellular flow cytometric analysis of IFN-γproducing CD8 + T cells and 51 Cr release assay. (A) Representative intracellular IFN-γ flow cytometric data obtained from HIV-1-positive participant #1. (B) Summary data from intracellular IFN-γ flow cytometry of HIV-1-positive participant #2–#4 are graphically depicted. Open bars represent CD4 + T cell-containing condition and dark bar represent CD4 + T cell -depleted conditions. (C) A representative 51 Cr release assay result from HIV-1-positive participant #1 is shown. Similar results were obtained with HIV-1-positive participant #2 (data not shown). (D) Summary data from intracellular IFN-γ flow cytometric analysis of EBV-positive participant #5–#7 are graphically depicted. Open bars represent CD4 + T cell -containing condition and dark bars represent CD4 + T cell -depleted conditions. (E) A representative 51 Cr release assay result from EBV-positive participant #6 is shown. Similar results were obtained with EBV-positive participant #5 (data not shown). DC, MDDCs not pulsed with peptide; DCp, MDDCs pulsed with peptide; vA3131-2/DCp, vA3131-2-infected MDDCs pulsed with peptide; ALVAC/DCp, parental ALVAC II-infected MDDCs pulsed with peptide.

    Journal: Vaccine

    Article Title: CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in-vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals

    doi: 10.1016/j.vaccine.2008.05.018

    Figure Lengend Snippet: CD40L expressed by recombinant ALVAC virus enhances specific antiviral CTL activity. CD4 + T cell-containing or -depleted PBMCs from four HIV-1-infected individuals and three HIV-1-uninfected individuals were cocultured with autologous MDDCs that were either pulsed or not with HLA-restricted epitopes of HIV-1 proteins (for HIV-1 infected individuals) or EBV proteins (for HIV-1-uninfected individuals). MDDCs were previously infected or not (medium) with parental ALVAC II or ALVAC recombinant vA3131-2 expressing human CD40L at an MOI of 10 for 48 h. On day 10, specific CTL activity was assessed by intracellular flow cytometric analysis of IFN-γproducing CD8 + T cells and 51 Cr release assay. (A) Representative intracellular IFN-γ flow cytometric data obtained from HIV-1-positive participant #1. (B) Summary data from intracellular IFN-γ flow cytometry of HIV-1-positive participant #2–#4 are graphically depicted. Open bars represent CD4 + T cell-containing condition and dark bar represent CD4 + T cell -depleted conditions. (C) A representative 51 Cr release assay result from HIV-1-positive participant #1 is shown. Similar results were obtained with HIV-1-positive participant #2 (data not shown). (D) Summary data from intracellular IFN-γ flow cytometric analysis of EBV-positive participant #5–#7 are graphically depicted. Open bars represent CD4 + T cell -containing condition and dark bars represent CD4 + T cell -depleted conditions. (E) A representative 51 Cr release assay result from EBV-positive participant #6 is shown. Similar results were obtained with EBV-positive participant #5 (data not shown). DC, MDDCs not pulsed with peptide; DCp, MDDCs pulsed with peptide; vA3131-2/DCp, vA3131-2-infected MDDCs pulsed with peptide; ALVAC/DCp, parental ALVAC II-infected MDDCs pulsed with peptide.

    Article Snippet: For flow cytometry, human peripheral blood mononuclear cells (PBMCs) were infected with recombinant viruses or ALVAC II at 5 MOI for 24 h and then stained with PE-conjugated anti-mouse or anti-human CD40L (BD Biosciences, Mississauga, ON, Canada).

    Techniques: Recombinant, CTL Assay, Activity Assay, Infection, Expressing, Flow Cytometry, Release Assay, Cytometry

    Expression of murine CD40L by recombinant canarypox vector. Recombinant canarypox viruses vCPmCD40L and vCPmSP-D-CD40L were generated as described in Materials and Methods. (A) RT-PCR. CEF cells were infected by vCPmCD40L, vCPmSP-D-CD40L or its parental control ALVAC II at 5 MOI for 3 days. Total RNA was isolated from infected CEF cells using Trizol Reagent and subjected to RT-PCR with specific primers amplifying the coding sequence of murine CD40L or SP-D-CD40L. The identity of the amplified product was further verified by DNA sequencing (not shown). (B) Flow cytometry. Human PBMCs were infected by vCPmCD40L, vCPmSP-D-CD40L or ALVAC II at 5 MOI for 24 hrs and stained with PE-labeled anti-mouse CD40L mAb. (C) Western blot. Hela cells were infected by vCPmCD40L, vCPmSP-D-CD40L, or ALVAC II at 10 MOI for 24–48hrs. Cell lysates or magnetic beads incubated with supernatant of infected cells were subjected to Western blot and detected with a goat anti-mouse CD40L or anti-mouse SP-D antibody. As expected, the membrane CD40L was about 35–40kD and soluble multimeric form of CD40L was about 70kD.

    Journal: Vaccine

    Article Title: CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in-vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals

    doi: 10.1016/j.vaccine.2008.05.018

    Figure Lengend Snippet: Expression of murine CD40L by recombinant canarypox vector. Recombinant canarypox viruses vCPmCD40L and vCPmSP-D-CD40L were generated as described in Materials and Methods. (A) RT-PCR. CEF cells were infected by vCPmCD40L, vCPmSP-D-CD40L or its parental control ALVAC II at 5 MOI for 3 days. Total RNA was isolated from infected CEF cells using Trizol Reagent and subjected to RT-PCR with specific primers amplifying the coding sequence of murine CD40L or SP-D-CD40L. The identity of the amplified product was further verified by DNA sequencing (not shown). (B) Flow cytometry. Human PBMCs were infected by vCPmCD40L, vCPmSP-D-CD40L or ALVAC II at 5 MOI for 24 hrs and stained with PE-labeled anti-mouse CD40L mAb. (C) Western blot. Hela cells were infected by vCPmCD40L, vCPmSP-D-CD40L, or ALVAC II at 10 MOI for 24–48hrs. Cell lysates or magnetic beads incubated with supernatant of infected cells were subjected to Western blot and detected with a goat anti-mouse CD40L or anti-mouse SP-D antibody. As expected, the membrane CD40L was about 35–40kD and soluble multimeric form of CD40L was about 70kD.

    Article Snippet: For flow cytometry, human peripheral blood mononuclear cells (PBMCs) were infected with recombinant viruses or ALVAC II at 5 MOI for 24 h and then stained with PE-conjugated anti-mouse or anti-human CD40L (BD Biosciences, Mississauga, ON, Canada).

    Techniques: Expressing, Recombinant, Plasmid Preparation, Generated, Reverse Transcription Polymerase Chain Reaction, Infection, Isolation, Sequencing, Amplification, DNA Sequencing, Flow Cytometry, Cytometry, Staining, Labeling, Western Blot, Magnetic Beads, Incubation

    Detection of ApoE isoforms in differentiating hNP1 cells. Differentiating hNP1 cells were exposed to medium alone (Unt), mock-infected PBMC supernatants (Mock), and HIV-infected PBMC supernatants (HIV), and to rApoE3 or rApoE4 at 3 μg/ml, as described in Materials and Methods. At day 16 of differentiation, and 24 h after replenishment of rApoE in the cultures, cells were lysed for quantitative immunoblotting to detect ApoE. Lower blot depicts representative endogenous ApoE detected in cell culture lysates with molecular weight of 34 kDa, and rApoE with molecular weight of 36 kDa. Upper blot corresponds to beta-actin used as an internal control. For comparison, recombinant ApoE3 and ApoE4 were loaded directly on the gel at a 3 ng per lane, as shown. Bar graphs present ApoE signal normalized to beta-actin; each bar represents the mean of two experiments

    Journal: Journal of Neuroimmune Pharmacology

    Article Title: Apolipoprotein E4 Suppresses Neuronal-Specific Gene Expression in Maturing Neuronal Progenitor Cells Exposed to HIV

    doi: 10.1007/s11481-017-9734-9

    Figure Lengend Snippet: Detection of ApoE isoforms in differentiating hNP1 cells. Differentiating hNP1 cells were exposed to medium alone (Unt), mock-infected PBMC supernatants (Mock), and HIV-infected PBMC supernatants (HIV), and to rApoE3 or rApoE4 at 3 μg/ml, as described in Materials and Methods. At day 16 of differentiation, and 24 h after replenishment of rApoE in the cultures, cells were lysed for quantitative immunoblotting to detect ApoE. Lower blot depicts representative endogenous ApoE detected in cell culture lysates with molecular weight of 34 kDa, and rApoE with molecular weight of 36 kDa. Upper blot corresponds to beta-actin used as an internal control. For comparison, recombinant ApoE3 and ApoE4 were loaded directly on the gel at a 3 ng per lane, as shown. Bar graphs present ApoE signal normalized to beta-actin; each bar represents the mean of two experiments

    Article Snippet: “HIV-Exposed” HIV-1 containing supernatants were obtained from human peripheral blood mononuclear cells (PBMC) that were stimulated with mitogens phytohemagglutinin (PHA, Sigma-Aldrich, St Lois, MO) and recombinant human interleukin-2 (IL-2, Roche Diagnostics, Indianapolis, IN), then infected with HIV-1 as described previously (McCarthy et al. ).

    Techniques: Infection, Cell Culture, Molecular Weight, Recombinant

    Binding of CSL to human PBMCs and inhibition of binding with competing glycoconjugates. PBMCs were stained with FITC-labeled CSL and subjected to flow cytometric analysis. X -axis, FL1-H on a log scale represents the fluorescence intensity of cells stained with FITC labeled CSL. Y -axis represents cell number. (a) The histoplot shows profiles of the unstained cells (UNS) and cells stained with FITC-labeled CSL (CSL). Profiles of cells stained with FITC-labeled CSL preincubated with different haptens are indicated in (b, c and d).

    Journal: Biochemistry Research International

    Article Title: Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis

    doi: 10.1155/2010/854656

    Figure Lengend Snippet: Binding of CSL to human PBMCs and inhibition of binding with competing glycoconjugates. PBMCs were stained with FITC-labeled CSL and subjected to flow cytometric analysis. X -axis, FL1-H on a log scale represents the fluorescence intensity of cells stained with FITC labeled CSL. Y -axis represents cell number. (a) The histoplot shows profiles of the unstained cells (UNS) and cells stained with FITC-labeled CSL (CSL). Profiles of cells stained with FITC-labeled CSL preincubated with different haptens are indicated in (b, c and d).

    Article Snippet: Binding of CSL to Human PBMCs Human peripheral blood mononuclear cells (PBMCs) were isolated from the blood of healthy donors by density gradient using Histopaque-1077 (Sigma) and resuspended in complete medium (RPMI 1640 + 10% FCS).

    Techniques: Binding Assay, Inhibition, Staining, Labeling, Flow Cytometry, Fluorescence

    Mitogenic activity of CSL. PBMCs were isolated from blood of healthy donors and exposed to serial concentrations of CSL (0.625–10 μ g/ml) and PHA-L (0.16–2.5 μ g/ml) for 72 hours and proliferation was measured by tritiated thymidine incorporation assay as counts per minute (CPM). The data are presented as mean ± SE of four independent experiments done in triplicates.

    Journal: Biochemistry Research International

    Article Title: Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis

    doi: 10.1155/2010/854656

    Figure Lengend Snippet: Mitogenic activity of CSL. PBMCs were isolated from blood of healthy donors and exposed to serial concentrations of CSL (0.625–10 μ g/ml) and PHA-L (0.16–2.5 μ g/ml) for 72 hours and proliferation was measured by tritiated thymidine incorporation assay as counts per minute (CPM). The data are presented as mean ± SE of four independent experiments done in triplicates.

    Article Snippet: Binding of CSL to Human PBMCs Human peripheral blood mononuclear cells (PBMCs) were isolated from the blood of healthy donors by density gradient using Histopaque-1077 (Sigma) and resuspended in complete medium (RPMI 1640 + 10% FCS).

    Techniques: Activity Assay, Isolation, Thymidine Incorporation Assay

    ShK-186 inhibits antineutrophil cytoplasmic antibodies targeting proteinase 3 (PR3-ANCA) production in vitro . (A) IgG production after peripheral blood mononuclear cell (PBMC) stimulation with CpG, B cell-activating factor (BAFF), and interleukin (IL)-21 from 5 healthy controls (HCs) (open circles) and 13 granulomatosis with polyangiitis (GPA) patients (gray circles) in the presence and absence of 1 nM ShK-186. (B) PR3-ANCA production after PBMC stimulation with CpG, BAFF, and IL-21 from 13 GPA patients in the presence and absence of 1 nM ShK-186 (* p

    Journal: Frontiers in Immunology

    Article Title: Kv1.3 Channel Blockade Modulates the Effector Function of B Cells in Granulomatosis with Polyangiitis

    doi: 10.3389/fimmu.2017.01205

    Figure Lengend Snippet: ShK-186 inhibits antineutrophil cytoplasmic antibodies targeting proteinase 3 (PR3-ANCA) production in vitro . (A) IgG production after peripheral blood mononuclear cell (PBMC) stimulation with CpG, B cell-activating factor (BAFF), and interleukin (IL)-21 from 5 healthy controls (HCs) (open circles) and 13 granulomatosis with polyangiitis (GPA) patients (gray circles) in the presence and absence of 1 nM ShK-186. (B) PR3-ANCA production after PBMC stimulation with CpG, BAFF, and IL-21 from 13 GPA patients in the presence and absence of 1 nM ShK-186 (* p

    Article Snippet: Briefly, peripheral blood mononuclear cells (PBMCs) were isolated from GPA patients, who produce ANCA upon in vitro induction , and stored in RPMI 1640 (Lonza, Basel, Switzerland) supplemented with 50 µg/mL gentamycin (GIBCO, Life Technologies, Grand Island, NY, USA), 10% fetal calf serum (FCS, Lonza), and 10% dimethyl sulfoxide (DMSO).

    Techniques: In Vitro

    ADCC of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of PBMC effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.

    Journal: PLoS ONE

    Article Title: Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey

    doi: 10.1371/journal.pone.0196422

    Figure Lengend Snippet: ADCC of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of PBMC effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.

    Article Snippet: ADCC assay Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 (DIACLONE Research) and 55 μM 2-mercaptoethanol for 24 hours.

    Techniques: Multiple Displacement Amplification, Labeling, Incubation