Journal: PLoS ONE

Article Title: Immunization with a streptococcal multiple-epitope recombinant protein protects mice against invasive group A streptococcal infection

doi: 10.1371/journal.pone.0174464

Figure Lengend Snippet: (A) Competitive inhibition of fibronectin binding by anti-rFSBM serum. Sera from rFSBM-immunized mice or CFA adjuvant-only control mice were incubated with emm1 -type GAS for 1 h and then added to rhodamine-conjugated fibronectin for another 1 h. The MFI of the GAS was measured using a flow cytometer. (B) The rFSBM-immunized serum or the CFA adjuvant-only control serum was incubated with emm1 -type GAS and then used to infect A549 or HMEC-1 cells. The unbound bacteria were removed by washing with sterile PBS and the total number of bacteria bound with the cells was quantified by plating on THY agar plates. * P < 0.05, ** P < 0.01, compared with the values determined for the CFA adjuvant-only control serum. (C) The anti-rFSBM serum or CFA adjuvant-only control serum was incubated with streptolysin S and mouse RBC for 1 h, and the percentage of hemolysis was measured using an ELISA reader, as described in the Materials and Methods. * P < 0.05, compared with the values determined for the control serum.

Article Snippet: Human epithelial A549 cells and HMEC-1 cells were cultured in DMEM containing 5% FCS and Medium 200 containing low serum growth supplement (Thermo Fisher Scientific), respectively.

Techniques: Inhibition, Binding Assay, Incubation, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Antiviral research

Article Title: Inhibition of adenovirus replication by a trisubstituted piperazin-2-one derivative

doi: 10.1016/j.antiviral.2014.05.010

Figure Lengend Snippet: Dose-dependent activity of 15D8 against Ad5-GFP (A) and Ad16-GFP (B) in a high MOI single round infection assay using A549 cell. Antiviral effects of compound 15D8 at low MOI on Ad5 (C) and Ad16 (D) in a plaque assay using the 293β5 cell line. For all panels, the negative control (−) is non-infected cells, while the positive control (+) is cells infected at the same MOI but in absence of compound. Data are presented as the mean ± SD from triplicate assays.

Article Snippet: High-throughput screening (HTS) of combinatorial chemical libraries An initial rapid screening was performed using human A549 epithelial cells (3 × 10 5 cells/well in Corning black wall, clear bottom 96-well plates) infected with Ad5-GFP or Ad16-GFP (2,000 vp/cell) in the presence of 50 μM of the candidate antiviral compounds.

Techniques: Activity Assay, Infection, Plaque Assay, Negative Control, Positive Control