Journal: Cell Metabolism

Article Title: The mitochondrial pyruvate carrier regulates memory T cell differentiation and antitumor function

doi: 10.1016/j.cmet.2022.03.013

Figure Lengend Snippet: MPC inhibition dramatically improves human CD19-CAR T cell therapy in a xenograft leukemia model (A) Experimental scheme. (B) Fold expansion after 9 days of culture (n = 3 human donors/group). (C–E) Percentage CD62L-positive CD8 + T cells (C) and median fluorescent intensity (MFI) of CD62L in the CD62L-positive population (D), and representative histogram from one donor (E). (F) Percentage of stem cell-like memory CD8 + T cells, measured by flow cytometry. In (C)–(F), n = 7 human donors/group; pooled data from 2 independent experiments. (G) Western blot quantification of H3K27 acetylation shown as fold change compared with DMSO (n = 5 human donors/group; pooled data from 2 independent experiments). (H) Experimental scheme. (I and J) Histogram of CD62L expression (I) and FACS plot showing CD45-RO and CCR7 expression (J) in CD8 + T cells from a representative donor, 7 days after transduction (EM, effector memory; CM, central memory; SCM, stem cell-like memory). (K) Overall survival. (L) Number of NALM6 cells in the blood (n = 4 mice for untreated, n = 5 mice for NTD DMSO and NTD MPCi, n = 7 mice for CAR DMSO, and n = 8 mice for CAR MPCi; pooled data from 2 independent experiments). Data are represented as mean ± SEM. Statistics are based on paired, two-tailed Student’s t test (B–D and F–G), log rank test (K), or two-way ANOVA using Fisher’s LSD test (L), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, or as indicated. See also Figure S7 .

Article Snippet: B16-F10 melanoma cells, Phoenix-Eco cells and the human B cell leukemia cell line NALM6 cells were originally acquired from the American Type Culture Collection.

Techniques: Inhibition, Flow Cytometry, Western Blot, Expressing, Transduction, Two Tailed Test

Journal: Cell Metabolism

Article Title: The mitochondrial pyruvate carrier regulates memory T cell differentiation and antitumor function

doi: 10.1016/j.cmet.2022.03.013

Figure Lengend Snippet:

Article Snippet: B16-F10 melanoma cells, Phoenix-Eco cells and the human B cell leukemia cell line NALM6 cells were originally acquired from the American Type Culture Collection.

Techniques: Staining, Recombinant, Expressing, Lysis, Selection, Purification, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Magnetic Beads, Western Blot, Sequencing, Software

Journal: iScience

Article Title: TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

doi: 10.1016/j.isci.2023.106543

Figure Lengend Snippet:

Article Snippet: Human: NALM-6 , DSMZ , Cat#ACC 128.

Techniques: Purification, shRNA, Transduction, Recombinant, Reporter Assay, Lysis, Plasmid Preparation, Software