human iga immunoglobulin a elisa kit Search Results


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  • 93
    Thermo Fisher enzyme linked immunosorbent assay elisa
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ORGENTEC Diagnostika GmbH enzyme linked immunosorbent assay elisa
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    Bethyl iga elisa quantitation kit
    Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and <t>IgA</t> against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P
    Iga Elisa Quantitation Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 88/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shino-Test Corporation enzyme linked immunosorbent assay elisa
    Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and <t>IgA</t> against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Shino-Test Corporation, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems human lbp duoset enzyme linked immunosorbent assay elisa
    Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and <t>IgA</t> against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P
    Human Lbp Duoset Enzyme Linked Immunosorbent Assay Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    USCN Life enzyme linked immunosorbent assay elisa kit
    Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and <t>IgA</t> against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P
    Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by USCN Life, used in various techniques. Bioz Stars score: 94/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bethyl enzyme linked immunosorbent assay elisa kit
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher enzyme linked immunosorbent assay
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tecan Systems enzyme linked immunosorbent assay elisa kit
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Phoenix Pharmaceuticals pylori enzyme linked immunosorbent assay elisa kit
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Pylori Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Phoenix Pharmaceuticals, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mab Technologies sandwich enzyme linked immunosorbent assay elisa kit
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Sandwich Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Mab Technologies, used in various techniques. Bioz Stars score: 91/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GTI Inc enzyme linked immunosorbent assay elisa kit pak 2mp
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa Kit Pak 2mp, supplied by GTI Inc, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher aβ40 enzyme linked immunosorbent assay elisa kits
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Aβ40 Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam enzyme linked immunosorbent assay elisa
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Salimetrics LLC enzyme linked immunosorbent assay elisa
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Salimetrics LLC, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Sciences Inc enzyme linked immunosorbent assay elisa
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Cell Sciences Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bethyl human igg enzyme linked immunosorbent assay elisa kits
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Human Igg Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inova Diagnostics iga httg enzyme linked immunosorbent assay kit
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Iga Httg Enzyme Linked Immunosorbent Assay Kit, supplied by Inova Diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    USCN Life enzyme linked immunosorbent assay elisa
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa, supplied by USCN Life, used in various techniques. Bioz Stars score: 94/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
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    Bio-Rad enzyme linked immunosorbent assay elisa assay
    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma of engrafted NSG mice at 12 weeks. ** P
    Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Adipogen, used in various techniques. Bioz Stars score: 92/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of rTp0965 on chemoattraction of monocytes to HUVECs. (A) Confluent HUVECs in the wells were preincubated with rTp0965 (800 ng/ml) for 24 h. Monocyte THP-1 cells stained with calcein AM were then added in the inserts for 2 h and the percentage of THP-1 cells in the wells were counted under the fluorescence microscope. (B and C) Confluent HUVECs were incubated with various concentrations of the rTp0965 for increasing time intervals. The levels of <t>MCP-1</t> in the supernatants were determined by <t>ELISA.</t> (D and E) Confluent HUVECs were incubated with various concentrations of rTp0965 for increasing time intervals. The levels of MCP-1 mRNA in HUVECs were measured by RT-PCR. (* = P
    Human Mcp 1 Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ZeptoMetrix igg enzyme linked immunosorbent assay elisa kits
    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G <t>(IgG)</t> antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked <t>immunosorbent</t> spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay <t>(ELISA)</t> with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.
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    Inova Diagnostics enzyme linked immunosorbent assays
    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G <t>(IgG)</t> antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked <t>immunosorbent</t> spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay <t>(ELISA)</t> with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.
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    Image Search Results


    Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P

    Journal: Frontiers in Immunology

    Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins

    doi: 10.3389/fimmu.2017.00953

    Figure Lengend Snippet: Mucosal antibody levels against Moraxella catarrhalis ( Mcat ) proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. Mcat protein-specific (A) IgG and (B) IgA in nasal wash were compared between 238 healthy visits of 59 sOP children and 270 healthy visits of 75 NOP children. IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are represented as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparisons. * P

    Article Snippet: The levels of IgG and IgA in the reference serum were quantitatively measured by using a human IgG or IgA ELISA quantitation kit (Bethyl laboratories).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Comparison of mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins between visits with and without Mcat nasopharyngeal (NP) colonization in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 healthy visits with Mcat NP colonization positive and 131 healthy visits with Mcat NP colonization negative of 59 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 healthy visits with Mcat NP colonization positive and 140 healthy visits with Mcat NP colonization negative of 75 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

    Journal: Frontiers in Immunology

    Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins

    doi: 10.3389/fimmu.2017.00953

    Figure Lengend Snippet: Comparison of mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins between visits with and without Mcat nasopharyngeal (NP) colonization in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 healthy visits with Mcat NP colonization positive and 131 healthy visits with Mcat NP colonization negative of 59 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 healthy visits with Mcat NP colonization positive and 140 healthy visits with Mcat NP colonization negative of 75 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

    Article Snippet: The levels of IgG and IgA in the reference serum were quantitatively measured by using a human IgG or IgA ELISA quantitation kit (Bethyl laboratories).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins at healthy visits with Mcat nasopharyngeal (NP) colonization vs. acute otitis media (AOM) visits in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 Mcat NP colonization visits of 47 sOP children and 42 Mcat -caused AOM visits of 31 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 Mcat NP colonization visits of 61 NOP children and 43 Mcat -caused AOM visits of 37 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

    Journal: Frontiers in Immunology

    Article Title: Stringently Defined Otitis Prone Children Demonstrate Deficient Naturally Induced Mucosal Antibody Response to Moraxella catarrhalis Proteins

    doi: 10.3389/fimmu.2017.00953

    Figure Lengend Snippet: Mucosal antibodies to Moraxella catarrhalis ( Mcat ) proteins at healthy visits with Mcat nasopharyngeal (NP) colonization vs. acute otitis media (AOM) visits in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children. Nasal wash IgG and IgA against Mcat proteins outer membrane protein (OMP) CD, oligopeptide permease A (OppA), Hag5–9, and Pilin A clade 2 (PilA2) were detected by using enzyme-linked immunosorbent assay for children age 6–36 months old. (A,B) 107 Mcat NP colonization visits of 47 sOP children and 42 Mcat -caused AOM visits of 31 sOP children were analyzed for nasal wash (A) IgG and (B) IgA. (C,D) 130 Mcat NP colonization visits of 61 NOP children and 43 Mcat -caused AOM visits of 37 NOP children were analyzed for nasal wash (C) IgG and (D) IgA. Mcat protein-specific IgG and IgA concentrations (ng/ml) were normalized with total IgG and IgA concentrations (μg/ml) in nasal wash, respectively, for comparison. Data are shown as geometric mean ± 95% confidence interval. Mann–Whitney test was used for comparison. * P

    Article Snippet: The levels of IgG and IgA in the reference serum were quantitatively measured by using a human IgG or IgA ELISA quantitation kit (Bethyl laboratories).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked immunosorbent assay (ELISA) in plasma of engrafted NSG mice at 12 weeks. ** P

    Journal: Clinical and Experimental Immunology

    Article Title: Human immune system development and survival of non-obese diabetic (NOD)-scid IL2rγnull (NSG) mice engrafted with human thymus and autologous haematopoietic stem cells

    doi: 10.1111/cei.12180

    Figure Lengend Snippet: Subcutaneous implantation of human thymic tissue leads to reduced human T cell generation. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space or subcutaneously. All implanted mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human CD3-depleted fetal liver. The percentages of human CD45 + cells (a) CD3 + cells (b) and CD20 + cells in the blood and spleen is shown from mice at 18 weeks post-implant. Thymus/liver grafts in NSG mice implanted in the subcapsular space (d,e,h,i) or subcutaneously in mice irradiated with 200 cGy (f,g,j,k) were evaluated by haematoxylin and eosin (H E) staining (d,e,f,g) and by immunostaining for human CD45 (h,i,j,k) at 18 weeks post-implant. The original magnifications were either ×15 (d,h,f,j) or ×630 (e,i,g,k). Levels of human IgM (l) and human IgG (m) were quantified by enzyme-linked immunosorbent assay (ELISA) in plasma of engrafted NSG mice at 12 weeks. ** P

    Article Snippet: Human IgM and IgG levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Bethyl Laboratories, Inc., Montgomery, TX, USA) according to the manufacturer's instructions and an EMax Endpoint ELISA microplate reader (Molecular Devices, Sunnyvale, CA, USA).

    Techniques: Mouse Assay, Irradiation, Injection, Derivative Assay, Staining, Immunostaining, Enzyme-linked Immunosorbent Assay

    The engrafted human immune system in bone marrow, liver, thymus (BLT) mice undergoes spontaneous activation at late points after implant. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space and then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human fetal liver. The percentages of human CD45 + cells (a), human CD3 + cells (b) and human CD20 + cells (c) in the blood and spleen is shown from mice at 26–28 weeks post-implant. At 12 and 26 weeks (d) human CD4 + and CD8 + T cells in the peripheral blood were examined for the expression of CD45RA. The values shown represent the percentage of human CD4 + or CD8 + T cells co-expressing CD45RA. The percentage of human CD4 + or CD8 + T cells expressing CD45RA in the spleen (e) is shown at 26–28 weeks post-implant. Levels (ng/ml) of human immunoglobulin (Ig)M (f) and human IgG (g) were quantified by enzyme-linked immunosorbent assay (ELISA) in plasma of engrafted NSG mice at the indicated times after implant. Haematological analyses (h, platelets; i, red blood cells; j, haemoglobin) of blood from NSG–BLT mice were performed over time as described in Materials and methods. * P

    Journal: Clinical and Experimental Immunology

    Article Title: Human immune system development and survival of non-obese diabetic (NOD)-scid IL2rγnull (NSG) mice engrafted with human thymus and autologous haematopoietic stem cells

    doi: 10.1111/cei.12180

    Figure Lengend Snippet: The engrafted human immune system in bone marrow, liver, thymus (BLT) mice undergoes spontaneous activation at late points after implant. Non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice were irradiated with 200 cGy, implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space and then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + haematopoietic stem cells (HSC) derived from the autologous human fetal liver. The percentages of human CD45 + cells (a), human CD3 + cells (b) and human CD20 + cells (c) in the blood and spleen is shown from mice at 26–28 weeks post-implant. At 12 and 26 weeks (d) human CD4 + and CD8 + T cells in the peripheral blood were examined for the expression of CD45RA. The values shown represent the percentage of human CD4 + or CD8 + T cells co-expressing CD45RA. The percentage of human CD4 + or CD8 + T cells expressing CD45RA in the spleen (e) is shown at 26–28 weeks post-implant. Levels (ng/ml) of human immunoglobulin (Ig)M (f) and human IgG (g) were quantified by enzyme-linked immunosorbent assay (ELISA) in plasma of engrafted NSG mice at the indicated times after implant. Haematological analyses (h, platelets; i, red blood cells; j, haemoglobin) of blood from NSG–BLT mice were performed over time as described in Materials and methods. * P

    Article Snippet: Human IgM and IgG levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Bethyl Laboratories, Inc., Montgomery, TX, USA) according to the manufacturer's instructions and an EMax Endpoint ELISA microplate reader (Molecular Devices, Sunnyvale, CA, USA).

    Techniques: Mouse Assay, Activation Assay, Irradiation, Injection, Derivative Assay, Expressing, Enzyme-linked Immunosorbent Assay

    Irradiation is not required for human B cell development in haematopoietic stem cells (HSC)-engrafted non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice implanted with human thymic tissues but enhances immunoglobulin (Ig) production. NSG mice were either irradiated with 200 cGy or not irradiated (0 cGy) and mice from each group were then implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space (thymic implant) or left unmanipulated (no thymic implant). All mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + HSC derived from the autologous human fetal liver. The peripheral blood of recipient NSG mice was screened for human CD20 + B cell levels (percentage of human CD45 + cells) at 12 weeks after implant (a). At 16 weeks after implant the level of human CD20 + B cells was determined in the blood (b) and spleen (c,d). Levels of human IgM (e) and human IgG (f) were quantified by enzyme-linked immunosorbent assay (ELISA) in plasma of engrafted NSG mice at 12 weeks. * P

    Journal: Clinical and Experimental Immunology

    Article Title: Human immune system development and survival of non-obese diabetic (NOD)-scid IL2rγnull (NSG) mice engrafted with human thymus and autologous haematopoietic stem cells

    doi: 10.1111/cei.12180

    Figure Lengend Snippet: Irradiation is not required for human B cell development in haematopoietic stem cells (HSC)-engrafted non-obese diabetic (NOD) -scid IL2rγ null (NSG) mice implanted with human thymic tissues but enhances immunoglobulin (Ig) production. NSG mice were either irradiated with 200 cGy or not irradiated (0 cGy) and mice from each group were then implanted with 1 mm 3 fragments of human fetal thymus and liver in the renal subcapsular space (thymic implant) or left unmanipulated (no thymic implant). All mice were then injected intravenously with 1 × 10 5 to 5 × 10 5 CD34 + HSC derived from the autologous human fetal liver. The peripheral blood of recipient NSG mice was screened for human CD20 + B cell levels (percentage of human CD45 + cells) at 12 weeks after implant (a). At 16 weeks after implant the level of human CD20 + B cells was determined in the blood (b) and spleen (c,d). Levels of human IgM (e) and human IgG (f) were quantified by enzyme-linked immunosorbent assay (ELISA) in plasma of engrafted NSG mice at 12 weeks. * P

    Article Snippet: Human IgM and IgG levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Bethyl Laboratories, Inc., Montgomery, TX, USA) according to the manufacturer's instructions and an EMax Endpoint ELISA microplate reader (Molecular Devices, Sunnyvale, CA, USA).

    Techniques: Irradiation, Mouse Assay, Injection, Derivative Assay, Enzyme-linked Immunosorbent Assay

    Effects of rTp0965 on chemoattraction of monocytes to HUVECs. (A) Confluent HUVECs in the wells were preincubated with rTp0965 (800 ng/ml) for 24 h. Monocyte THP-1 cells stained with calcein AM were then added in the inserts for 2 h and the percentage of THP-1 cells in the wells were counted under the fluorescence microscope. (B and C) Confluent HUVECs were incubated with various concentrations of the rTp0965 for increasing time intervals. The levels of MCP-1 in the supernatants were determined by ELISA. (D and E) Confluent HUVECs were incubated with various concentrations of rTp0965 for increasing time intervals. The levels of MCP-1 mRNA in HUVECs were measured by RT-PCR. (* = P

    Journal: PLoS ONE

    Article Title: Recombinant Treponema pallidum Protein Tp0965 Activates Endothelial Cells and Increases the Permeability of Endothelial Cell Monolayer

    doi: 10.1371/journal.pone.0115134

    Figure Lengend Snippet: Effects of rTp0965 on chemoattraction of monocytes to HUVECs. (A) Confluent HUVECs in the wells were preincubated with rTp0965 (800 ng/ml) for 24 h. Monocyte THP-1 cells stained with calcein AM were then added in the inserts for 2 h and the percentage of THP-1 cells in the wells were counted under the fluorescence microscope. (B and C) Confluent HUVECs were incubated with various concentrations of the rTp0965 for increasing time intervals. The levels of MCP-1 in the supernatants were determined by ELISA. (D and E) Confluent HUVECs were incubated with various concentrations of rTp0965 for increasing time intervals. The levels of MCP-1 mRNA in HUVECs were measured by RT-PCR. (* = P

    Article Snippet: The human MCP-1 enzyme-linked immunosorbent assay (ELISA) kit was purchased from R & D Systems, Inc. (Minneapolis, MN).

    Techniques: Staining, Fluorescence, Microscopy, Incubation, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction

    Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked immunosorbent spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay (ELISA) with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.

    Journal: The Journal of Infectious Diseases

    Article Title: Distinct Cross-reactive B-Cell Responses to Live Attenuated and Inactivated Influenza Vaccines

    doi: 10.1093/infdis/jiu190

    Figure Lengend Snippet: Nuclear protein (NP)–specific plasmablast responses to live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV). A , Frequency of NP-specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibody-secreting cells (ASCs) (left panels) and percentage of NP-specific ASCs among total vaccine-specific ASCs (right panels) after immunization with 2011 LAIV or IIV. The NP-specific ASCs were measured with enzyme-linked immunosorbent spot plates coated with recombinant NP. B , Binding activity of NP-specific IgG plasmablast-derived polyclonal antibodies (PPAbs) from recipients of 2010 IIV or recipient of 2010 LAIV (left panel) and relative NP binding activity normalized to HA (H1N1) binding activity (right panel). The binding activity was calculated as the area under curve (AUC) of serially diluted PPAb samples, using an enzyme-linked immunosorbent assay (ELISA) with recombinant NP- or HA-coated ELISA plates, starting at a dilution of 1:100 for NP or 1:1000 for HA. Bars indicate geometric means. Means were compared between IIV and LAIV groups by an unpaired t test or by fitting a regression model, using the generalized maximum entropy approach.

    Article Snippet: The concentrations of IgA and IgG in PPAbs were determined with the Immuno-Tek Quantitative Human IgA or IgG enzyme-linked immunosorbent assay (ELISA) kits (Zeptometrix), respectively.

    Techniques: ELISpot Assay, Recombinant, Binding Assay, Activity Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay

    Cross-reactive hemagglutinin (HA) binding activity of plasmablast-derived polyclonal antibodies (PPAbs) after immunization with 2010 inactivated influenza vaccine (IIV) and live attenuated influenza vaccine (LAIV). A pool of PPAbs was assembled by combining equal amounts of immunoglobulin G (IgG) from each PPAb sample in the IIV or LAIV groups. Each PPAb pool was serially diluted, starting at a concentration of 9 ng/mL for LAIV and 4.1 ng/mL for IIV of IgG, and analyzed with an enzyme-linked immunosorbent assay (ELISA) for binding to recombinant HAs from indicated influenza virus strains. Relative binding activity to each HA (numbers in each panel) was defined as the ratio of its area under curve (AUC) to that of the relevant 2010 vaccine strain (circle in each panel, assigned a value of 1).

    Journal: The Journal of Infectious Diseases

    Article Title: Distinct Cross-reactive B-Cell Responses to Live Attenuated and Inactivated Influenza Vaccines

    doi: 10.1093/infdis/jiu190

    Figure Lengend Snippet: Cross-reactive hemagglutinin (HA) binding activity of plasmablast-derived polyclonal antibodies (PPAbs) after immunization with 2010 inactivated influenza vaccine (IIV) and live attenuated influenza vaccine (LAIV). A pool of PPAbs was assembled by combining equal amounts of immunoglobulin G (IgG) from each PPAb sample in the IIV or LAIV groups. Each PPAb pool was serially diluted, starting at a concentration of 9 ng/mL for LAIV and 4.1 ng/mL for IIV of IgG, and analyzed with an enzyme-linked immunosorbent assay (ELISA) for binding to recombinant HAs from indicated influenza virus strains. Relative binding activity to each HA (numbers in each panel) was defined as the ratio of its area under curve (AUC) to that of the relevant 2010 vaccine strain (circle in each panel, assigned a value of 1).

    Article Snippet: The concentrations of IgA and IgG in PPAbs were determined with the Immuno-Tek Quantitative Human IgA or IgG enzyme-linked immunosorbent assay (ELISA) kits (Zeptometrix), respectively.

    Techniques: Binding Assay, Activity Assay, Derivative Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Recombinant