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    Abcam human neutrophil elastase
    Human Neutrophil Elastase, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore human histone h1 dna
    Confirmation of decoration of the extracellular trap (ET)-like structures with characteristic protein signatures by immunostaining. Fluorescence microscopy images of neutrophil-enriched cell suspensions from Atlantic salmon cultured in vitro with 5 μg/ml CaI ( a – f ) or 10 nM PMA ( g – h ) for 1 h at 15 °C. a – c. Immunocytochemical detection of neutrophil elastase in ETotic neutrophils; scale bars, 50 μm. a. Localisation of neutrophil elastase by rabbit to human neutrophil elastase and stained with conjugated Alexa Fluor 488 (green). b. <t>DNA</t> stained blue with 4′,6-diamidino-2-phenylindole (DAPI). c . Merge of a–b. d – f. Immunocytochemical detection of histone H2A in different ETotic neutrophils; scale bars = 100 μm. d. Localisation of histone H2A by mouse to human histone H2A and stained with conjugated Alexa Fluor 488. e. DNA stained with DAPI. f. Merge of d–e. g – h. Immunocytochemical detection of histone <t>H1</t> with DNA in an extended extracellular strand interlinking two cells; scale bars, 100 μm. g. Localisation of H1 with DNA by mouse to human histone H1/DNA and stained with conjugated Alexa Fluor 488. h. DNA stained with DAPI. i. Merge of g–h. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Human Histone H1 Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PELOBIOTECH GmbH 466z022 human coronary artery endothelial cells hcaec pelobiotech pb ch 182 2011
    Confirmation of decoration of the extracellular trap (ET)-like structures with characteristic protein signatures by immunostaining. Fluorescence microscopy images of neutrophil-enriched cell suspensions from Atlantic salmon cultured in vitro with 5 μg/ml CaI ( a – f ) or 10 nM PMA ( g – h ) for 1 h at 15 °C. a – c. Immunocytochemical detection of neutrophil elastase in ETotic neutrophils; scale bars, 50 μm. a. Localisation of neutrophil elastase by rabbit to human neutrophil elastase and stained with conjugated Alexa Fluor 488 (green). b. <t>DNA</t> stained blue with 4′,6-diamidino-2-phenylindole (DAPI). c . Merge of a–b. d – f. Immunocytochemical detection of histone H2A in different ETotic neutrophils; scale bars = 100 μm. d. Localisation of histone H2A by mouse to human histone H2A and stained with conjugated Alexa Fluor 488. e. DNA stained with DAPI. f. Merge of d–e. g – h. Immunocytochemical detection of histone <t>H1</t> with DNA in an extended extracellular strand interlinking two cells; scale bars, 100 μm. g. Localisation of H1 with DNA by mouse to human histone H1/DNA and stained with conjugated Alexa Fluor 488. h. DNA stained with DAPI. i. Merge of g–h. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    466z022 Human Coronary Artery Endothelial Cells Hcaec Pelobiotech Pb Ch 182 2011, supplied by PELOBIOTECH GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PELOBIOTECH GmbH qc06814f10 human aortic endothelial cells haoec pelobiotech 304k 05a
    Confirmation of decoration of the extracellular trap (ET)-like structures with characteristic protein signatures by immunostaining. Fluorescence microscopy images of neutrophil-enriched cell suspensions from Atlantic salmon cultured in vitro with 5 μg/ml CaI ( a – f ) or 10 nM PMA ( g – h ) for 1 h at 15 °C. a – c. Immunocytochemical detection of neutrophil elastase in ETotic neutrophils; scale bars, 50 μm. a. Localisation of neutrophil elastase by rabbit to human neutrophil elastase and stained with conjugated Alexa Fluor 488 (green). b. <t>DNA</t> stained blue with 4′,6-diamidino-2-phenylindole (DAPI). c . Merge of a–b. d – f. Immunocytochemical detection of histone H2A in different ETotic neutrophils; scale bars = 100 μm. d. Localisation of histone H2A by mouse to human histone H2A and stained with conjugated Alexa Fluor 488. e. DNA stained with DAPI. f. Merge of d–e. g – h. Immunocytochemical detection of histone <t>H1</t> with DNA in an extended extracellular strand interlinking two cells; scale bars, 100 μm. g. Localisation of H1 with DNA by mouse to human histone H1/DNA and stained with conjugated Alexa Fluor 488. h. DNA stained with DAPI. i. Merge of g–h. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Qc06814f10 Human Aortic Endothelial Cells Haoec Pelobiotech 304k 05a, supplied by PELOBIOTECH GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Confirmation of decoration of the extracellular trap (ET)-like structures with characteristic protein signatures by immunostaining. Fluorescence microscopy images of neutrophil-enriched cell suspensions from Atlantic salmon cultured in vitro with 5 μg/ml CaI ( a – f ) or 10 nM PMA ( g – h ) for 1 h at 15 °C. a – c. Immunocytochemical detection of neutrophil elastase in ETotic neutrophils; scale bars, 50 μm. a. Localisation of neutrophil elastase by rabbit to human neutrophil elastase and stained with conjugated Alexa Fluor 488 (green). b. DNA stained blue with 4′,6-diamidino-2-phenylindole (DAPI). c . Merge of a–b. d – f. Immunocytochemical detection of histone H2A in different ETotic neutrophils; scale bars = 100 μm. d. Localisation of histone H2A by mouse to human histone H2A and stained with conjugated Alexa Fluor 488. e. DNA stained with DAPI. f. Merge of d–e. g – h. Immunocytochemical detection of histone H1 with DNA in an extended extracellular strand interlinking two cells; scale bars, 100 μm. g. Localisation of H1 with DNA by mouse to human histone H1/DNA and stained with conjugated Alexa Fluor 488. h. DNA stained with DAPI. i. Merge of g–h. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Fish & Shellfish Immunology

    Article Title: Release of chromatin extracellular traps by phagocytes of Atlantic salmon, Salmo salar (Linnaeus, 1758)

    doi: 10.1016/j.fsi.2021.08.023

    Figure Lengend Snippet: Confirmation of decoration of the extracellular trap (ET)-like structures with characteristic protein signatures by immunostaining. Fluorescence microscopy images of neutrophil-enriched cell suspensions from Atlantic salmon cultured in vitro with 5 μg/ml CaI ( a – f ) or 10 nM PMA ( g – h ) for 1 h at 15 °C. a – c. Immunocytochemical detection of neutrophil elastase in ETotic neutrophils; scale bars, 50 μm. a. Localisation of neutrophil elastase by rabbit to human neutrophil elastase and stained with conjugated Alexa Fluor 488 (green). b. DNA stained blue with 4′,6-diamidino-2-phenylindole (DAPI). c . Merge of a–b. d – f. Immunocytochemical detection of histone H2A in different ETotic neutrophils; scale bars = 100 μm. d. Localisation of histone H2A by mouse to human histone H2A and stained with conjugated Alexa Fluor 488. e. DNA stained with DAPI. f. Merge of d–e. g – h. Immunocytochemical detection of histone H1 with DNA in an extended extracellular strand interlinking two cells; scale bars, 100 μm. g. Localisation of H1 with DNA by mouse to human histone H1/DNA and stained with conjugated Alexa Fluor 488. h. DNA stained with DAPI. i. Merge of g–h. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Specifically, these were mouse to human histone H2A (L88A6) (#3636; Cell Signaling Technology, London, UK), mouse to human histone H1/DNA (MAB3864; Millipore, Watford, UK), and rabbit to human neutrophil elastase (ab21595; Abcam, Cambridge, UK).

    Techniques: Immunostaining, Fluorescence, Microscopy, Cell Culture, In Vitro, Staining