Journal: Scientific Reports

Article Title: Rapid DNA visual detection of polymicrobial bloodstream infection using filter paper

doi: 10.1038/s41598-022-08487-4

Figure Lengend Snippet: Detection with different types of targets. ( a ) Signal intensities of multiplex PCR products (FV, VB, FB, and FVB) amplified from genomic DNA. ( b ) Signal intensities from a single PCR product (F, V, and B) to a mixture of two or three single PCR products (FV, VB, FB, or FVB) amplified from spiked DNA. ( c ) Visual detection using multiplex PCR products amplified from spiked DNA. All the differences of intensities between the specific and the NTC signals on the same filter paper were significant. X axes of 3a and 3b represent the targets of detection. Y axes display the signal intensities. F: Saccharomyces cerevisiae . V: human cytomegalovirus. B: Escherichia coli . NTC: negative control. * p ≤ 0.05 using student t-test (F, V, and B) or p ≤ 0.05/comparison times using post hoc test (FV, VB, FB, or FVB).

Article Snippet: HCMV gDNA was ordered from ATCC.

Techniques: Multiplex Assay, Amplification, Negative Control

Journal: Scientific Reports

Article Title: Rapid DNA visual detection of polymicrobial bloodstream infection using filter paper

doi: 10.1038/s41598-022-08487-4

Figure Lengend Snippet: Detection limit (DL) analysis of the functionalized filter paper based on PCR products amplified from genomic DNA. F, V, or B represents a single PCR product. FV, VB, or FB is the corresponding duplex PCR product. FVB means the triplex PCR product. The signal intensities of blue columns represent detection outcome when 10 ng of each target was used, and the pink ones represent the results when 50 ng of each target was used. Every signal intensity was compared to that of NTC on the same paper slide. The work was done in triplicate. The DL was between 0.1 ng/µL and 0.5 ng/µL. F: Saccharomyces cerevisiae . V: human cytomegalovirus. B: Escherichia coli . NTC: negative control. * p ≤ 0.05 using student t-test (F, V, and B) or p ≤ 0.05/comparison times using post hoc test (FV, VB, FB, or FVB). NS: no significant difference.

Article Snippet: HCMV gDNA was ordered from ATCC.

Techniques: Amplification, Negative Control