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    Full length Clone DNA of Human acyl CoA thioesterase 8 with C terminal Flag tag
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    99
    Thermo Fisher human cot 1 dna
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot 1 Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies human cot 1 dna
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot 1 Dna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cot 1 dna/product/Agilent technologies
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    92
    ATUM human cot 1 dna
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot 1 Dna, supplied by ATUM, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abbott Laboratories human cot 1 dna
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot 1 Dna, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cot 1 dna/product/Abbott Laboratories
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    99
    Millipore human cot 1 dna
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot 1 Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cot 1 dna/product/Millipore
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    94
    Thermo Fisher human cot 1 dna fluorometric qc
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot 1 Dna Fluorometric Qc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore human cot1 dna
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot1 Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cot1 dna/product/Millipore
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    human cot1 dna - by Bioz Stars, 2020-12
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    92
    Boehringer Mannheim human cot 1 dna
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot 1 Dna, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human cot 1 dna - by Bioz Stars, 2020-12
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    92
    Stratagene human cot 1 dna
    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. <t>Cot-1</t> blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).
    Human Cot 1 Dna, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cot 1 dna/product/Stratagene
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    Image Search Results


    Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. Cot-1 blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).

    Journal: PLoS ONE

    Article Title: Fast and Non-Toxic In Situ Hybridization without Blocking of Repetitive Sequences

    doi: 10.1371/journal.pone.0040675

    Figure Lengend Snippet: Effect of EC on hybridization rate. Time chase comparison of FISH signal intensities using 15% EC buffer and 45% formamide buffer on FFPE breast carcinoma tissue sections. Cot-1 blocking was used in formamide buffer, but omitted for EC buffer. Identical probe concentration was used in the two buffers. EC buffer was denatured at 67°C for 10 minutes, formamide buffer at 82°C for 5 minutes, and then both hybridized at 45°C from t = 0 minute to t = 23 hours. A: Merged micrograph of red HER2 DNA probe signals, green CEN-17 PNA signals and blue DAPI staining ( Figure S2 ). The images are taken with identical exposure times. Scale bar, 10 µm. B: Quantitative analysis of HER2 DNA and C, of CEN-17 PNA signals ( Figures S2 and S3 ) . MFI, mean fluorescence intensity. The bars represent the 95% confidence interval (n = 45 signals).

    Article Snippet: The 45% formamide and solvent buffer consisted of: 45% v/v formamide or solvent; 10% v/v dextran sulfate; 0.1 µg/µL Human Cot-1 (15279-011, Invitrogen); 300 mM NaCl; 5 mM phosphate buffer; pH 7.5.

    Techniques: Hybridization, Fluorescence In Situ Hybridization, Formalin-fixed Paraffin-Embedded, Blocking Assay, Concentration Assay, Staining, Fluorescence

    FISH stains using same buffer component concentrations for EC and formamide buffers. BCL2 in: A: 15% formamide buffer, B: 15% EC buffer and C: 45% formamide buffer with Cot-1 blocking, with different denaturation conditions on FFPE breast carcinoma tissue sections. Merge of green BCL2 DNA, red BCL2 DNA split probe signals and of blue DAPI staining. Denatured at 67°C for 10 minutes or 82°C for 5 minutes and hybridized at 45°C for 60 minutes. The images are taken with identical exposure times. Scale bar, 10 µm.

    Journal: PLoS ONE

    Article Title: Fast and Non-Toxic In Situ Hybridization without Blocking of Repetitive Sequences

    doi: 10.1371/journal.pone.0040675

    Figure Lengend Snippet: FISH stains using same buffer component concentrations for EC and formamide buffers. BCL2 in: A: 15% formamide buffer, B: 15% EC buffer and C: 45% formamide buffer with Cot-1 blocking, with different denaturation conditions on FFPE breast carcinoma tissue sections. Merge of green BCL2 DNA, red BCL2 DNA split probe signals and of blue DAPI staining. Denatured at 67°C for 10 minutes or 82°C for 5 minutes and hybridized at 45°C for 60 minutes. The images are taken with identical exposure times. Scale bar, 10 µm.

    Article Snippet: The 45% formamide and solvent buffer consisted of: 45% v/v formamide or solvent; 10% v/v dextran sulfate; 0.1 µg/µL Human Cot-1 (15279-011, Invitrogen); 300 mM NaCl; 5 mM phosphate buffer; pH 7.5.

    Techniques: Fluorescence In Situ Hybridization, Blocking Assay, Formalin-fixed Paraffin-Embedded, Staining

    Examples of alternative solvents for FISH hybridization buffer. A: sulfolane. B: γ-butyrolactone. C: Ethylene carbonate (EC). D: 2-pyrrolidone. E: δ-valerolactam. F: EC. The images in A-C are merged micrographs of Texas Red-labeled BCL2 DNA and green fluorescein-labeled BCL2 DNA split probes, and in D-F are of Texas Red-labeled HER2 DNA and green fluorescein-labeled CEN-17 PNA solid tumor FISH probes on FFPE breast carcinoma tissue sections. The sections were denatured at 67°C for 10 minutes and hybridized at 45°C for 60 minutes with FISH buffers containing 15% solvent. Cot-1 blocking was omitted. All images are taken with same exposure times. Blue, DAPI stain. Scale bar, 10 µm.

    Journal: PLoS ONE

    Article Title: Fast and Non-Toxic In Situ Hybridization without Blocking of Repetitive Sequences

    doi: 10.1371/journal.pone.0040675

    Figure Lengend Snippet: Examples of alternative solvents for FISH hybridization buffer. A: sulfolane. B: γ-butyrolactone. C: Ethylene carbonate (EC). D: 2-pyrrolidone. E: δ-valerolactam. F: EC. The images in A-C are merged micrographs of Texas Red-labeled BCL2 DNA and green fluorescein-labeled BCL2 DNA split probes, and in D-F are of Texas Red-labeled HER2 DNA and green fluorescein-labeled CEN-17 PNA solid tumor FISH probes on FFPE breast carcinoma tissue sections. The sections were denatured at 67°C for 10 minutes and hybridized at 45°C for 60 minutes with FISH buffers containing 15% solvent. Cot-1 blocking was omitted. All images are taken with same exposure times. Blue, DAPI stain. Scale bar, 10 µm.

    Article Snippet: The 45% formamide and solvent buffer consisted of: 45% v/v formamide or solvent; 10% v/v dextran sulfate; 0.1 µg/µL Human Cot-1 (15279-011, Invitrogen); 300 mM NaCl; 5 mM phosphate buffer; pH 7.5.

    Techniques: Fluorescence In Situ Hybridization, Hybridization, Labeling, Formalin-fixed Paraffin-Embedded, Blocking Assay, Staining

    AR FISH on normal female metaphase spread slides. Slides were hybridized with TAMRA labeled AR probe with and without repeats and hybridized with and without the presence of C 0 t -1 in the hybmix. ( A ) RF AR probe hybridized with 25× excess C 0 t -1 DNA. ( B ) RC AR probe hybridized with 25× excess C 0 t -1 DNA. ( C ) RF AR probe hybridized without blocking DNA. ( D ) RC AR probe hybridized without blocking DNA. The two histograms show the line intensity profiles of the lines in the pictures. The top histogram shows the profiles of the lines from image A and B to which C 0 t -1 DNA was added and the bottom histograms show the profiles of the lines in image C and D to which no C 0 t -1 DNA was added.

    Journal: Nucleic Acids Research

    Article Title: Construction of repeat-free fluorescence in situ hybridization probes

    doi: 10.1093/nar/gkr1123

    Figure Lengend Snippet: AR FISH on normal female metaphase spread slides. Slides were hybridized with TAMRA labeled AR probe with and without repeats and hybridized with and without the presence of C 0 t -1 in the hybmix. ( A ) RF AR probe hybridized with 25× excess C 0 t -1 DNA. ( B ) RC AR probe hybridized with 25× excess C 0 t -1 DNA. ( C ) RF AR probe hybridized without blocking DNA. ( D ) RC AR probe hybridized without blocking DNA. The two histograms show the line intensity profiles of the lines in the pictures. The top histogram shows the profiles of the lines from image A and B to which C 0 t -1 DNA was added and the bottom histograms show the profiles of the lines in image C and D to which no C 0 t -1 DNA was added.

    Article Snippet: Fifty nanogram of this material was added to a 40× excess of C 0 t -1 DNA (Invitrogen, Carlsbad, CA, USA, cat. 15279-011) in 300 mM NaCl in a total volume of 10 µl and denatured for 10 min at 95°C.

    Techniques: Fluorescence In Situ Hybridization, Labeling, Blocking Assay

    AR FISH on paraffin-embedded MCF-7 cells. Five micrometer thick fixed paraffin-embedded MCF7 cells were hybridized with TAMRA labeled AR probe with and without repeats and hybridized with and without the presence of C 0 t -1 in the hybmix. ( A ) RF AR probe hybridized with 25× excess C 0 t -1 DNA. ( B ) RC AR probe hybridized with 25× excess C 0 t -1 DNA. ( C ) RF AR probe hybridized without blocking DNA. ( D ) RC AR probe hybridized without blocking DNA. The two histograms show the line intensity profiles of the lines in the pictures. The top histogram shows the profiles of the lines from image A and B to which C 0 t -1 DNA was added and the bottom histograms show the profiles of the lines in image C and D to which no C 0 t -1 DNA was added.

    Journal: Nucleic Acids Research

    Article Title: Construction of repeat-free fluorescence in situ hybridization probes

    doi: 10.1093/nar/gkr1123

    Figure Lengend Snippet: AR FISH on paraffin-embedded MCF-7 cells. Five micrometer thick fixed paraffin-embedded MCF7 cells were hybridized with TAMRA labeled AR probe with and without repeats and hybridized with and without the presence of C 0 t -1 in the hybmix. ( A ) RF AR probe hybridized with 25× excess C 0 t -1 DNA. ( B ) RC AR probe hybridized with 25× excess C 0 t -1 DNA. ( C ) RF AR probe hybridized without blocking DNA. ( D ) RC AR probe hybridized without blocking DNA. The two histograms show the line intensity profiles of the lines in the pictures. The top histogram shows the profiles of the lines from image A and B to which C 0 t -1 DNA was added and the bottom histograms show the profiles of the lines in image C and D to which no C 0 t -1 DNA was added.

    Article Snippet: Fifty nanogram of this material was added to a 40× excess of C 0 t -1 DNA (Invitrogen, Carlsbad, CA, USA, cat. 15279-011) in 300 mM NaCl in a total volume of 10 µl and denatured for 10 min at 95°C.

    Techniques: Fluorescence In Situ Hybridization, Labeling, Blocking Assay