human cell line 293 Search Results


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    • human kidney fibroblast cell line 293  (ATCC)
    93
    ATCC human kidney fibroblast cell line 293
    Human Kidney Fibroblast Cell Line 293, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human kidney fibroblast cell line 293/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human kidney fibroblast cell line 293 - by Bioz Stars, 2023-09
    93/100 stars
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    hek293  (ATCC)
    94
    ATCC hek293
    ( A ) Interface between RET and GFRAL. An overall view of the complex structure is shown in the middle as a reference. ( B ) Interface between RET-CRD and GDF15. ( C ) Pull-down assays for the GDF15/GFRAL/RET ternary complex. Strep-tagged GFRAL bound to Streptavidin-conjugated beads was used to pull down GDF15 and RET. Streptavidin beads without Strep-GFRAL bound served as negative control. Mutations in either GFRAL or GDF15 abolish binding of RET. The results shown are representative of three biological repeats. ( D ) GDF15-induced ERK phosphorylation in <t>HEK293</t> cells expressing full-length myc-tagged RET and GFRAL. Cells were treated with GDF15 at 10 nM for 15 min. ERK phosphorylation levels (pERK) were assessed by western blot. Expression levels of RET and GFRAL were monitored by anti-myc western blot. The results shown are representative of three biological repeats.
    Hek293, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek293 - by Bioz Stars, 2023-09
    94/100 stars
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    control hek293 cell line  (ATCC)
    97
    ATCC control hek293 cell line
    ( A ) Interface between RET and GFRAL. An overall view of the complex structure is shown in the middle as a reference. ( B ) Interface between RET-CRD and GDF15. ( C ) Pull-down assays for the GDF15/GFRAL/RET ternary complex. Strep-tagged GFRAL bound to Streptavidin-conjugated beads was used to pull down GDF15 and RET. Streptavidin beads without Strep-GFRAL bound served as negative control. Mutations in either GFRAL or GDF15 abolish binding of RET. The results shown are representative of three biological repeats. ( D ) GDF15-induced ERK phosphorylation in <t>HEK293</t> cells expressing full-length myc-tagged RET and GFRAL. Cells were treated with GDF15 at 10 nM for 15 min. ERK phosphorylation levels (pERK) were assessed by western blot. Expression levels of RET and GFRAL were monitored by anti-myc western blot. The results shown are representative of three biological repeats.
    Control Hek293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control hek293 cell line/product/ATCC
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control hek293 cell line - by Bioz Stars, 2023-09
    97/100 stars
    		  Buy from Supplier
    kidney hek293 cells  (ATCC)
    99
    ATCC kidney hek293 cells
    ( A ) Interface between RET and GFRAL. An overall view of the complex structure is shown in the middle as a reference. ( B ) Interface between RET-CRD and GDF15. ( C ) Pull-down assays for the GDF15/GFRAL/RET ternary complex. Strep-tagged GFRAL bound to Streptavidin-conjugated beads was used to pull down GDF15 and RET. Streptavidin beads without Strep-GFRAL bound served as negative control. Mutations in either GFRAL or GDF15 abolish binding of RET. The results shown are representative of three biological repeats. ( D ) GDF15-induced ERK phosphorylation in <t>HEK293</t> cells expressing full-length myc-tagged RET and GFRAL. Cells were treated with GDF15 at 10 nM for 15 min. ERK phosphorylation levels (pERK) were assessed by western blot. Expression levels of RET and GFRAL were monitored by anti-myc western blot. The results shown are representative of three biological repeats.
    Kidney Hek293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kidney hek293 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kidney hek293 cells - by Bioz Stars, 2023-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Interface between RET and GFRAL. An overall view of the complex structure is shown in the middle as a reference. ( B ) Interface between RET-CRD and GDF15. ( C ) Pull-down assays for the GDF15/GFRAL/RET ternary complex. Strep-tagged GFRAL bound to Streptavidin-conjugated beads was used to pull down GDF15 and RET. Streptavidin beads without Strep-GFRAL bound served as negative control. Mutations in either GFRAL or GDF15 abolish binding of RET. The results shown are representative of three biological repeats. ( D ) GDF15-induced ERK phosphorylation in HEK293 cells expressing full-length myc-tagged RET and GFRAL. Cells were treated with GDF15 at 10 nM for 15 min. ERK phosphorylation levels (pERK) were assessed by western blot. Expression levels of RET and GFRAL were monitored by anti-myc western blot. The results shown are representative of three biological repeats.

    Journal: eLife

    Article Title: Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands

    doi: 10.7554/eLife.47650

    Figure Lengend Snippet: ( A ) Interface between RET and GFRAL. An overall view of the complex structure is shown in the middle as a reference. ( B ) Interface between RET-CRD and GDF15. ( C ) Pull-down assays for the GDF15/GFRAL/RET ternary complex. Strep-tagged GFRAL bound to Streptavidin-conjugated beads was used to pull down GDF15 and RET. Streptavidin beads without Strep-GFRAL bound served as negative control. Mutations in either GFRAL or GDF15 abolish binding of RET. The results shown are representative of three biological repeats. ( D ) GDF15-induced ERK phosphorylation in HEK293 cells expressing full-length myc-tagged RET and GFRAL. Cells were treated with GDF15 at 10 nM for 15 min. ERK phosphorylation levels (pERK) were assessed by western blot. Expression levels of RET and GFRAL were monitored by anti-myc western blot. The results shown are representative of three biological repeats.

    Article Snippet: Cell line ( Homo sapiens ) , HEK293 , ATCC , Cat# PTA-4488, RRID: CVCL_0045 , .

    Techniques: Negative Control, Binding Assay, Expressing, Western Blot

    Journal: eLife

    Article Title: Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands

    doi: 10.7554/eLife.47650

    Figure Lengend Snippet:

    Article Snippet: Cell line ( Homo sapiens ) , HEK293 , ATCC , Cat# PTA-4488, RRID: CVCL_0045 , .

    Techniques: Transfection, Construct, Plasmid Preparation, Expressing, Purification, Phosphorylation Assay, Software, Imaging