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Journal: Molecular Human Reproduction
Article Title: Differential tissue-specific damage caused by bacterial epididymo-orchitis in the mouse
doi: 10.1093/molehr/gaaa011
Figure Lengend Snippet: RT-qPCR analysis and comparison of modulation of antimicrobial factors in caput and cauda epididymidis and Lypd8 assessment in mouse and in vitro assay. ( A ) beta-defensins, ( B ) UPEC-associated antimicrobial factors. Unpaired t test ( * ) or Mann–Whitney U test (#) for each organ, respectively, comparing sham to UPEC-infected mice ( **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05). Fold change calculated via 2 (−ddCT) method. Data presented as box-and-whisker with median and min/max. cap = caput, cau = cauda. Sham 10 days, caput n = 4, cauda n = 4; UPEC 10 days, caput n = 4, cauda n = 6. ( C ) Laser-assisted microdissection of caput epithelium and caput interstitium from wild-type epididymis, n = 2. Relative expression calculated via 2 (−dCT) method. Data presented as mean ± SD. ( D ) Bacterial adherence to MEPC5 cells under the presence (+) or absence (−) of 1 μg/ml human recombinant LYPD8 (hLYPD8). CFU/ml were calculated after incubation of agar plates at 37°C for 24 h. Summary of three independent experiments with each time n = 2–3 per group. Unpaired t test comparing the number of MEPC5-adherent UPEC in the presence or absence of LYPD8 ( **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05).
Article Snippet: To assess the effects of LYPD8 on bacterial adherence to epididymal epithelial cells in vitro , UPEC were pre-incubated for 1 h on ice with or without 1 μg/ml
Techniques: Quantitative RT-PCR, Comparison, In Vitro, MANN-WHITNEY, Infection, Whisker Assay, Laser Capture Microdissection, Expressing, Recombinant, Incubation