human bone marrow derived mscs Search Results


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  • 96
    Lonza hbm mscs
    Distribution of Cq values. (A) Adipo-, (B) chondro-, (C) osteogenesis. Left, values of all donors/passages in control and differentiation culture. Right, data split up into control (grey) and differentiation (black) cultures. (D) All lineages together. (E) RPL13a values of freshly isolated <t>(hBM-MSCs),</t> commercial bone marrow- (Lonza MSCs) and placenta-derived MSCs (hPD-MSCs) as well as HBCs and MG-63. Cq, quantification cycle values; EF1α, eukaryotic translational elongation factor 1 alpha; B2M, β2-microglobulin; ACTB, beta actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; hBM-MSCs, human bone marrow-derived MSCs; hPD-MSCs, human placenta-derived MSCs; HBCs, human bone cells; MG-63, human osteosarcoma cell line.
    Hbm Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbm mscs/product/Lonza
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbm mscs - by Bioz Stars, 2021-06
    96/100 stars
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    98
    Lonza human mesenchymal stem cells hmscs
    Distribution of Cq values. (A) Adipo-, (B) chondro-, (C) osteogenesis. Left, values of all donors/passages in control and differentiation culture. Right, data split up into control (grey) and differentiation (black) cultures. (D) All lineages together. (E) RPL13a values of freshly isolated <t>(hBM-MSCs),</t> commercial bone marrow- (Lonza MSCs) and placenta-derived MSCs (hPD-MSCs) as well as HBCs and MG-63. Cq, quantification cycle values; EF1α, eukaryotic translational elongation factor 1 alpha; B2M, β2-microglobulin; ACTB, beta actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; hBM-MSCs, human bone marrow-derived MSCs; hPD-MSCs, human placenta-derived MSCs; HBCs, human bone cells; MG-63, human osteosarcoma cell line.
    Human Mesenchymal Stem Cells Hmscs, supplied by Lonza, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mesenchymal stem cells hmscs/product/Lonza
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human mesenchymal stem cells hmscs - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

    97
    PromoCell hbm mscs
    Biocompatibility of PCL and bioactive scaffold for <t>hBM-MSCs</t> after 7 days of culture. HE staining ( A , B ), LIVE/DEAD viability test ( C , D ) and SEM observation ( E , F ). PCL and BMP-2 were not toxic to hBM-MSCs.
    Hbm Mscs, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbm mscs/product/PromoCell
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbm mscs - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

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    Human Bone Marrow Derived Stem Cell Frozen Vial and Plated cells are available Cells are only guaranteed with purchase of AcceGen Biotech Media and AcceGen Biotech Extra Cellular Matrix for
      Buy from Supplier

    N/A
    Human Mesenchymal Bone Marrow Adult Derived Stem Cells Frozen Vial and Plated cells are available Cells are only guaranteed with purchase of AcceGen Biotech Media and AcceGen Biotech Extra Cellular
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    GFP Expressing Human Bone Marrow Derived Mesenchymal Stem Cells are selected from puromycin resistant Human Bone Marrow Derived Mesenchymal Stem Cells after transfected with GFP expressing lentiviral particles
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    Image Search Results


    Distribution of Cq values. (A) Adipo-, (B) chondro-, (C) osteogenesis. Left, values of all donors/passages in control and differentiation culture. Right, data split up into control (grey) and differentiation (black) cultures. (D) All lineages together. (E) RPL13a values of freshly isolated (hBM-MSCs), commercial bone marrow- (Lonza MSCs) and placenta-derived MSCs (hPD-MSCs) as well as HBCs and MG-63. Cq, quantification cycle values; EF1α, eukaryotic translational elongation factor 1 alpha; B2M, β2-microglobulin; ACTB, beta actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; hBM-MSCs, human bone marrow-derived MSCs; hPD-MSCs, human placenta-derived MSCs; HBCs, human bone cells; MG-63, human osteosarcoma cell line.

    Journal: Tissue Engineering. Part C, Methods

    Article Title: Ribosomal Protein L13a as a Reference Gene for Human Bone Marrow-Derived Mesenchymal Stromal Cells During Expansion, Adipo-, Chondro-, and Osteogenesis

    doi: 10.1089/ten.tec.2012.0081

    Figure Lengend Snippet: Distribution of Cq values. (A) Adipo-, (B) chondro-, (C) osteogenesis. Left, values of all donors/passages in control and differentiation culture. Right, data split up into control (grey) and differentiation (black) cultures. (D) All lineages together. (E) RPL13a values of freshly isolated (hBM-MSCs), commercial bone marrow- (Lonza MSCs) and placenta-derived MSCs (hPD-MSCs) as well as HBCs and MG-63. Cq, quantification cycle values; EF1α, eukaryotic translational elongation factor 1 alpha; B2M, β2-microglobulin; ACTB, beta actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; hBM-MSCs, human bone marrow-derived MSCs; hPD-MSCs, human placenta-derived MSCs; HBCs, human bone cells; MG-63, human osteosarcoma cell line.

    Article Snippet: Once RPL13a was selected as the RG of choice for hBM-MSCs, we further tested its expression stability on commercially available hBM-MSCs (Lonza MSCs) as well as hPD-MSCs during adipo-, chondro-, and osteogenesis.

    Techniques: Isolation, Derivative Assay, Activation Assay

    Biocompatibility of PCL and bioactive scaffold for hBM-MSCs after 7 days of culture. HE staining ( A , B ), LIVE/DEAD viability test ( C , D ) and SEM observation ( E , F ). PCL and BMP-2 were not toxic to hBM-MSCs.

    Journal: Nanomaterials

    Article Title: A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

    doi: 10.3390/nano10091774

    Figure Lengend Snippet: Biocompatibility of PCL and bioactive scaffold for hBM-MSCs after 7 days of culture. HE staining ( A , B ), LIVE/DEAD viability test ( C , D ) and SEM observation ( E , F ). PCL and BMP-2 were not toxic to hBM-MSCs.

    Article Snippet: Cell Culture The hBM-MSCs (Promocell, Heidelberg, Germany) ranging from passage 4 to 6 were grown at 37 °C in a humidified atmosphere of 5% CO2 in ready-to-use Mesenchymal Stem Cell Growth Medium 2 (Promocell, Heidelberg, Germany).

    Techniques: Staining

    Observation of bone regeneration of the maxillary bone after 90 days of implantation of a PCL membrane functionalized with BMP-2 ( A – C , E ) and non-functionalized ( A , B , D , F ) with hBM-MSCs. A 3D reconstruction ( A ) of frontal ( B ) and sagittal ( C , D ) sections of X-ray microtomography and Gomori trichrome staining ( E , F ).

    Journal: Nanomaterials

    Article Title: A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

    doi: 10.3390/nano10091774

    Figure Lengend Snippet: Observation of bone regeneration of the maxillary bone after 90 days of implantation of a PCL membrane functionalized with BMP-2 ( A – C , E ) and non-functionalized ( A , B , D , F ) with hBM-MSCs. A 3D reconstruction ( A ) of frontal ( B ) and sagittal ( C , D ) sections of X-ray microtomography and Gomori trichrome staining ( E , F ).

    Article Snippet: Cell Culture The hBM-MSCs (Promocell, Heidelberg, Germany) ranging from passage 4 to 6 were grown at 37 °C in a humidified atmosphere of 5% CO2 in ready-to-use Mesenchymal Stem Cell Growth Medium 2 (Promocell, Heidelberg, Germany).

    Techniques: Staining

    ( A ) Colony forming unit fibroblasts test (CFU-F): staining with HE of CFU formed after 14 days in culture. Arrows indicate 2 colonies. ( B ) Osteogenic differentiation after 21 days in the osteogenic medium; the differentiated cells produced nodules of mineralization stained with Alizarin Red S (arrows). ( C ) Adipocyte differentiation after 21 days in an adipogenic medium. Differentiated cells produced lipid vacuoles stained with Oil Red O. ( D ) Chondrocyte differentiation of the micromasses of hBM-MSCs after 21 days. Alcian Blue visualized the glycosaminoglycans (GAGs) specifically present in the cartilage.

    Journal: Nanomaterials

    Article Title: A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

    doi: 10.3390/nano10091774

    Figure Lengend Snippet: ( A ) Colony forming unit fibroblasts test (CFU-F): staining with HE of CFU formed after 14 days in culture. Arrows indicate 2 colonies. ( B ) Osteogenic differentiation after 21 days in the osteogenic medium; the differentiated cells produced nodules of mineralization stained with Alizarin Red S (arrows). ( C ) Adipocyte differentiation after 21 days in an adipogenic medium. Differentiated cells produced lipid vacuoles stained with Oil Red O. ( D ) Chondrocyte differentiation of the micromasses of hBM-MSCs after 21 days. Alcian Blue visualized the glycosaminoglycans (GAGs) specifically present in the cartilage.

    Article Snippet: Cell Culture The hBM-MSCs (Promocell, Heidelberg, Germany) ranging from passage 4 to 6 were grown at 37 °C in a humidified atmosphere of 5% CO2 in ready-to-use Mesenchymal Stem Cell Growth Medium 2 (Promocell, Heidelberg, Germany).

    Techniques: Staining, Produced

    Observation of bone regeneration of the maxillary bone 150 days after implantation of a PCL membrane functionalized with BMP-2 ( A , B , D ) and non-functionalized ( A , C , E ) with hBM-MSCs. A 3D reconstruction ( A ) of frontal and sagittal sections of X-ray microtomography and after microtome cutting and Gomori trichrome staining ( B – E ).

    Journal: Nanomaterials

    Article Title: A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

    doi: 10.3390/nano10091774

    Figure Lengend Snippet: Observation of bone regeneration of the maxillary bone 150 days after implantation of a PCL membrane functionalized with BMP-2 ( A , B , D ) and non-functionalized ( A , C , E ) with hBM-MSCs. A 3D reconstruction ( A ) of frontal and sagittal sections of X-ray microtomography and after microtome cutting and Gomori trichrome staining ( B – E ).

    Article Snippet: Cell Culture The hBM-MSCs (Promocell, Heidelberg, Germany) ranging from passage 4 to 6 were grown at 37 °C in a humidified atmosphere of 5% CO2 in ready-to-use Mesenchymal Stem Cell Growth Medium 2 (Promocell, Heidelberg, Germany).

    Techniques: Staining

    Observation of bone regeneration of the maxillary bone after 120 days of implantation of a PCL membrane functionalized with BMP-2 ( A – C , E ) and non-functionalized ( A , B , D , F ) with hBM-MSCs. A 3D reconstruction ( A ) of frontal ( B ) and sagittal ( C , D ) sections of X-ray microtomography and Gomori trichrome staining ( E , F ).

    Journal: Nanomaterials

    Article Title: A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

    doi: 10.3390/nano10091774

    Figure Lengend Snippet: Observation of bone regeneration of the maxillary bone after 120 days of implantation of a PCL membrane functionalized with BMP-2 ( A – C , E ) and non-functionalized ( A , B , D , F ) with hBM-MSCs. A 3D reconstruction ( A ) of frontal ( B ) and sagittal ( C , D ) sections of X-ray microtomography and Gomori trichrome staining ( E , F ).

    Article Snippet: Cell Culture The hBM-MSCs (Promocell, Heidelberg, Germany) ranging from passage 4 to 6 were grown at 37 °C in a humidified atmosphere of 5% CO2 in ready-to-use Mesenchymal Stem Cell Growth Medium 2 (Promocell, Heidelberg, Germany).

    Techniques: Staining