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  • 91
    R&D Systems human annexin a4 antibody
    Human Annexin A4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human annexin a4 antibody/product/R&D Systems
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human annexin a4 antibody - by Bioz Stars, 2020-08
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    93
    R&D Systems polyclonal goat antibody against annexin a4
    Induction of HC genes in GPA hFIBs grown for two weeks in RA EGF. (A) Induction of MYOSIN VIIA protein expression visualized by immunocytochemistry. (B) Relative mRNA expression of the endogenous genes MYOSIN VIIA , POU4F3 and ESPIN , as measured by qPCR analysis. (C) Induction of <t>ANNEXIN</t> A4 and ESPIN protein expression visualized by immunocytochemistry. Note that due to the very high increase in mRNA levels in GPA-infected samples the low levels of mRNAs detected in RA EGF control samples were barely visualized in the graph in (B). Asterisks: p-value
    Polyclonal Goat Antibody Against Annexin A4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal goat antibody against annexin a4/product/R&D Systems
    Average 93 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    polyclonal goat antibody against annexin a4 - by Bioz Stars, 2020-08
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    91
    Novus Biologicals annexin a4 antibody
    Induction of HC genes in GPA hFIBs grown for two weeks in RA EGF. (A) Induction of MYOSIN VIIA protein expression visualized by immunocytochemistry. (B) Relative mRNA expression of the endogenous genes MYOSIN VIIA , POU4F3 and ESPIN , as measured by qPCR analysis. (C) Induction of <t>ANNEXIN</t> A4 and ESPIN protein expression visualized by immunocytochemistry. Note that due to the very high increase in mRNA levels in GPA-infected samples the low levels of mRNAs detected in RA EGF control samples were barely visualized in the graph in (B). Asterisks: p-value
    Annexin A4 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin a4 antibody/product/Novus Biologicals
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    annexin a4 antibody - by Bioz Stars, 2020-08
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    92
    Proteintech polyclonal rabbit anti annexin iv ab
    <t>Anxa4</t> promotes airway epithelial progenitor cell migration. ( A ) Pregnant females carrying Shh CreER/+ ; Anxa4 F/+ ; Rosa26 -mTmG (control) and Shh CreER/+ ; Anxa4 F/F ; Rosa26 -mTmG ( Anxa4 cKO ) embryos were treated with tamoxifen (TAM) at E9.5 and lungs were imaged at E12.5. ( B ) Representative images of mosaic labeled control and Anxa4 cKO airway bud formation. One tip cells in control and Anxa4 cKO RCd.L1 buds are highlighted by blue and orange, respectively. Scale bar: 50 μm. ( C – F ) Cell track plots of tip cells in control lung (25 cells) and Anxa4 cKO lungs (16 cells) after aligning their starting positions ( C ), showing that Anxa4 cKO tip cells had shorter migration tracks and migration displacement than that of control tip cells ( C , D ); The migration velocity analysis showed that the migration velocity of Anxa4 cKO cells was decreased significantly ( E ) and the apical-to-basal cell length of Anxa4 cKO cells was similar to that of control cells ( F ). Data are presented as mean ± SEM; n = 3 samples per genotype; n.s., not significant; ***p
    Polyclonal Rabbit Anti Annexin Iv Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti annexin iv ab/product/Proteintech
    Average 92 stars, based on 3 article reviews
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    90
    Cusabio rabbit anti annexin a4
    <t>Anxa4</t> promotes airway epithelial progenitor cell migration. ( A ) Pregnant females carrying Shh CreER/+ ; Anxa4 F/+ ; Rosa26 -mTmG (control) and Shh CreER/+ ; Anxa4 F/F ; Rosa26 -mTmG ( Anxa4 cKO ) embryos were treated with tamoxifen (TAM) at E9.5 and lungs were imaged at E12.5. ( B ) Representative images of mosaic labeled control and Anxa4 cKO airway bud formation. One tip cells in control and Anxa4 cKO RCd.L1 buds are highlighted by blue and orange, respectively. Scale bar: 50 μm. ( C – F ) Cell track plots of tip cells in control lung (25 cells) and Anxa4 cKO lungs (16 cells) after aligning their starting positions ( C ), showing that Anxa4 cKO tip cells had shorter migration tracks and migration displacement than that of control tip cells ( C , D ); The migration velocity analysis showed that the migration velocity of Anxa4 cKO cells was decreased significantly ( E ) and the apical-to-basal cell length of Anxa4 cKO cells was similar to that of control cells ( F ). Data are presented as mean ± SEM; n = 3 samples per genotype; n.s., not significant; ***p
    Rabbit Anti Annexin A4, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti annexin a4/product/Cusabio
    Average 90 stars, based on 10 article reviews
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    rabbit anti annexin a4 - by Bioz Stars, 2020-08
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    Image Search Results


    Induction of HC genes in GPA hFIBs grown for two weeks in RA EGF. (A) Induction of MYOSIN VIIA protein expression visualized by immunocytochemistry. (B) Relative mRNA expression of the endogenous genes MYOSIN VIIA , POU4F3 and ESPIN , as measured by qPCR analysis. (C) Induction of ANNEXIN A4 and ESPIN protein expression visualized by immunocytochemistry. Note that due to the very high increase in mRNA levels in GPA-infected samples the low levels of mRNAs detected in RA EGF control samples were barely visualized in the graph in (B). Asterisks: p-value

    Journal: PLoS ONE

    Article Title: Transcription factor induced conversion of human fibroblasts towards the hair cell lineage

    doi: 10.1371/journal.pone.0200210

    Figure Lengend Snippet: Induction of HC genes in GPA hFIBs grown for two weeks in RA EGF. (A) Induction of MYOSIN VIIA protein expression visualized by immunocytochemistry. (B) Relative mRNA expression of the endogenous genes MYOSIN VIIA , POU4F3 and ESPIN , as measured by qPCR analysis. (C) Induction of ANNEXIN A4 and ESPIN protein expression visualized by immunocytochemistry. Note that due to the very high increase in mRNA levels in GPA-infected samples the low levels of mRNAs detected in RA EGF control samples were barely visualized in the graph in (B). Asterisks: p-value

    Article Snippet: Primary antibodies used in this work were: polyclonal rabbit antibody against MYOSIN VIIA (1:500; Proteus BioSciences, Inc., Ramona, CA, USA), mouse monoclonal antibody against POU4F3 (1:50; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, US), polyclonal goat antibody against GFI1 (1:50; R & D Systems, Minneapolis, MN, US), polyclonal goat antibody against ANNEXIN A4 (1:50; R & D Systems, Bio-Techne, Minneapolis, MN, USA) and polyclonal rabbit antibody against ESPIN (1:1000; kind gift from Professor James Hudspeth, New York, NY, USA).

    Techniques: Expressing, Immunocytochemistry, Real-time Polymerase Chain Reaction, Infection

    Anxa4 promotes airway epithelial progenitor cell migration. ( A ) Pregnant females carrying Shh CreER/+ ; Anxa4 F/+ ; Rosa26 -mTmG (control) and Shh CreER/+ ; Anxa4 F/F ; Rosa26 -mTmG ( Anxa4 cKO ) embryos were treated with tamoxifen (TAM) at E9.5 and lungs were imaged at E12.5. ( B ) Representative images of mosaic labeled control and Anxa4 cKO airway bud formation. One tip cells in control and Anxa4 cKO RCd.L1 buds are highlighted by blue and orange, respectively. Scale bar: 50 μm. ( C – F ) Cell track plots of tip cells in control lung (25 cells) and Anxa4 cKO lungs (16 cells) after aligning their starting positions ( C ), showing that Anxa4 cKO tip cells had shorter migration tracks and migration displacement than that of control tip cells ( C , D ); The migration velocity analysis showed that the migration velocity of Anxa4 cKO cells was decreased significantly ( E ) and the apical-to-basal cell length of Anxa4 cKO cells was similar to that of control cells ( F ). Data are presented as mean ± SEM; n = 3 samples per genotype; n.s., not significant; ***p

    Journal: Scientific Reports

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification

    doi: 10.1038/s41598-018-32494-z

    Figure Lengend Snippet: Anxa4 promotes airway epithelial progenitor cell migration. ( A ) Pregnant females carrying Shh CreER/+ ; Anxa4 F/+ ; Rosa26 -mTmG (control) and Shh CreER/+ ; Anxa4 F/F ; Rosa26 -mTmG ( Anxa4 cKO ) embryos were treated with tamoxifen (TAM) at E9.5 and lungs were imaged at E12.5. ( B ) Representative images of mosaic labeled control and Anxa4 cKO airway bud formation. One tip cells in control and Anxa4 cKO RCd.L1 buds are highlighted by blue and orange, respectively. Scale bar: 50 μm. ( C – F ) Cell track plots of tip cells in control lung (25 cells) and Anxa4 cKO lungs (16 cells) after aligning their starting positions ( C ), showing that Anxa4 cKO tip cells had shorter migration tracks and migration displacement than that of control tip cells ( C , D ); The migration velocity analysis showed that the migration velocity of Anxa4 cKO cells was decreased significantly ( E ) and the apical-to-basal cell length of Anxa4 cKO cells was similar to that of control cells ( F ). Data are presented as mean ± SEM; n = 3 samples per genotype; n.s., not significant; ***p

    Article Snippet: The primary antibodies and dilutions used were as follows: rabbit anti-p-ERK/12 (1:2500, #4370, CST); rabbit anti-ERK/12 (1:1000, #9102, CST); rabbit anti-Anxa4 (1:1000, 10087–1-AP, ProteinTech); mouse anti-b-Actin (1:2500, A2228, Sigma).

    Techniques: Migration, Labeling

    Anxa4 promotes distal airway epithelial cell fate commitment. ( A ) Pregnant females carrying Shh CreER/+ ; Anxa4 F/+ ; Rosa26 -mTmG (control) and Shh CreER/+ ; Anxa4 F/F ; Rosa26 -mTmG ( Anxa4 cKO ) embryos were treated with tamoxifen (TAM) at E9.5 and lungs were analyzed at different time point from E12.5 to E15.5. ( B , C ) Lungs at different time points from E12.5 to E15.5 were double stained for antibodies against GFP (green) and Sox9 (red), or GFP (green) and Sox2 (red) ( B ). Note that the proportion of GFP + cells in distal airways (Sox9 + ) of Anxa4 cKO lungs decreased over time as compared to that of control lungs, while the proportion of GFP + cells in stalk airways (Sox2 + ) of Anxa4 cKO lungs increased over time ( C ). Data are presented as mean ± S.E.M, n = 4. n.s., not significant; *p

    Journal: Scientific Reports

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification

    doi: 10.1038/s41598-018-32494-z

    Figure Lengend Snippet: Anxa4 promotes distal airway epithelial cell fate commitment. ( A ) Pregnant females carrying Shh CreER/+ ; Anxa4 F/+ ; Rosa26 -mTmG (control) and Shh CreER/+ ; Anxa4 F/F ; Rosa26 -mTmG ( Anxa4 cKO ) embryos were treated with tamoxifen (TAM) at E9.5 and lungs were analyzed at different time point from E12.5 to E15.5. ( B , C ) Lungs at different time points from E12.5 to E15.5 were double stained for antibodies against GFP (green) and Sox9 (red), or GFP (green) and Sox2 (red) ( B ). Note that the proportion of GFP + cells in distal airways (Sox9 + ) of Anxa4 cKO lungs decreased over time as compared to that of control lungs, while the proportion of GFP + cells in stalk airways (Sox2 + ) of Anxa4 cKO lungs increased over time ( C ). Data are presented as mean ± S.E.M, n = 4. n.s., not significant; *p

    Article Snippet: The primary antibodies and dilutions used were as follows: rabbit anti-p-ERK/12 (1:2500, #4370, CST); rabbit anti-ERK/12 (1:1000, #9102, CST); rabbit anti-Anxa4 (1:1000, 10087–1-AP, ProteinTech); mouse anti-b-Actin (1:2500, A2228, Sigma).

    Techniques: Staining

    Anxa4 functions redundantly with Anxa1 and Anxa6 in regulating endoderm budding process. ( A ) Relative expression level of Anxa1, Anxa2, Anxa3, Anxa5, Anxa6, Anxa7 and Anxa11 in control and Shh Cre/ + ; Anxa4 F/F lungs. ( B ) The relative expression level of Anxa1 , Anxa4 and Anxa6 at 48 h in cultured control lung endoderm treated with Scramble shRNA, or sh Anxa1 , or sh Anxa6 . The knockdown efficiency of sh Anxa1 or sh Anxa6 in cultured lung endoderm explants at 48 h was about 40%. Data are presented as mean ± S.E.M, n = 3. ***p

    Journal: Scientific Reports

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification

    doi: 10.1038/s41598-018-32494-z

    Figure Lengend Snippet: Anxa4 functions redundantly with Anxa1 and Anxa6 in regulating endoderm budding process. ( A ) Relative expression level of Anxa1, Anxa2, Anxa3, Anxa5, Anxa6, Anxa7 and Anxa11 in control and Shh Cre/ + ; Anxa4 F/F lungs. ( B ) The relative expression level of Anxa1 , Anxa4 and Anxa6 at 48 h in cultured control lung endoderm treated with Scramble shRNA, or sh Anxa1 , or sh Anxa6 . The knockdown efficiency of sh Anxa1 or sh Anxa6 in cultured lung endoderm explants at 48 h was about 40%. Data are presented as mean ± S.E.M, n = 3. ***p

    Article Snippet: The primary antibodies and dilutions used were as follows: rabbit anti-p-ERK/12 (1:2500, #4370, CST); rabbit anti-ERK/12 (1:1000, #9102, CST); rabbit anti-Anxa4 (1:1000, 10087–1-AP, ProteinTech); mouse anti-b-Actin (1:2500, A2228, Sigma).

    Techniques: Expressing, Cell Culture, shRNA

    ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant FGF10. ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p

    Journal: Scientific Reports

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification

    doi: 10.1038/s41598-018-32494-z

    Figure Lengend Snippet: ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant FGF10. ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p

    Article Snippet: The primary antibodies and dilutions used were as follows: rabbit anti-p-ERK/12 (1:2500, #4370, CST); rabbit anti-ERK/12 (1:1000, #9102, CST); rabbit anti-Anxa4 (1:1000, 10087–1-AP, ProteinTech); mouse anti-b-Actin (1:2500, A2228, Sigma).

    Techniques: Expressing, Cell Culture, Recombinant, Western Blot, Immunostaining, Staining, In Situ Hybridization