Journal: PLoS ONE
Article Title: Transcription factor induced conversion of human fibroblasts towards the hair cell lineage
Figure Lengend Snippet: Induction of HC genes in GPA hFIBs grown for two weeks in RA EGF. (A) Induction of MYOSIN VIIA protein expression visualized by immunocytochemistry. (B) Relative mRNA expression of the endogenous genes MYOSIN VIIA , POU4F3 and ESPIN , as measured by qPCR analysis. (C) Induction of ANNEXIN A4 and ESPIN protein expression visualized by immunocytochemistry. Note that due to the very high increase in mRNA levels in GPA-infected samples the low levels of mRNAs detected in RA EGF control samples were barely visualized in the graph in (B). Asterisks: p-value
Article Snippet: Primary antibodies used in this work were: polyclonal rabbit antibody against MYOSIN VIIA (1:500; Proteus BioSciences, Inc., Ramona, CA, USA), mouse monoclonal antibody against POU4F3 (1:50; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, US), polyclonal goat antibody against GFI1 (1:50; R & D Systems, Minneapolis, MN, US), polyclonal goat antibody against ANNEXIN A4 (1:50; R & D Systems, Bio-Techne, Minneapolis, MN, USA) and polyclonal rabbit antibody against ESPIN (1:1000; kind gift from Professor James Hudspeth, New York, NY, USA).
Techniques: Expressing, Immunocytochemistry, Real-time Polymerase Chain Reaction, Infection