human actionable solid tumor panel Search Results


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  • 99
    New England Biolabs direct cancer hotspot panel
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    Qiagen human actionable solid tumor panel kit
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    OriGene tissuescan oncology survey panel
    BCL family members are expressed in human solid tumor samples and in leukemia/lymphoma cell lines. ( a ) Expression of BCL genes in human solid tumor samples. <t>TissueScan</t> oncology survey cDNA panels were obtained from OriGene (Rockville, MD, USA) and quantitative PCR analysis was performed (Sybr Green, Applied Biosystems, Foster City, CA, USA) using two independent oligo pairs for each BCL gene. The average level of mRNA in normal tissue samples (light bars) was used for each tissue type to determine the fold-change in expression of each sample (dark bars). The average of the fold-change between the two pairs of gene specific oligos was determined and the standard error of the mean is depicted. The horizontal line indicating a three-fold increase in relative expression serves as a reference. ( b ) Antibodies specific for each BCL family member were used for western blot analysis of whole cell lysates (1% CHAPS, 150 mM NaCl, 10 mM Hepes) from multiple leukemia/lymphoma cell lines.. An antibody against MYC was used to determine the levels of MYC protein, antibodies against beta-actin and tubulin were used as a loading control.
    Tissuescan Oncology Survey Panel, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MRC-Holland salsa ms mlpa me042 c1 cimp kit
    BCL family members are expressed in human solid tumor samples and in leukemia/lymphoma cell lines. ( a ) Expression of BCL genes in human solid tumor samples. <t>TissueScan</t> oncology survey cDNA panels were obtained from OriGene (Rockville, MD, USA) and quantitative PCR analysis was performed (Sybr Green, Applied Biosystems, Foster City, CA, USA) using two independent oligo pairs for each BCL gene. The average level of mRNA in normal tissue samples (light bars) was used for each tissue type to determine the fold-change in expression of each sample (dark bars). The average of the fold-change between the two pairs of gene specific oligos was determined and the standard error of the mean is depicted. The horizontal line indicating a three-fold increase in relative expression serves as a reference. ( b ) Antibodies specific for each BCL family member were used for western blot analysis of whole cell lysates (1% CHAPS, 150 mM NaCl, 10 mM Hepes) from multiple leukemia/lymphoma cell lines.. An antibody against MYC was used to determine the levels of MYC protein, antibodies against beta-actin and tubulin were used as a loading control.
    Salsa Ms Mlpa Me042 C1 Cimp Kit, supplied by MRC-Holland, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BCL family members are expressed in human solid tumor samples and in leukemia/lymphoma cell lines. ( a ) Expression of BCL genes in human solid tumor samples. TissueScan oncology survey cDNA panels were obtained from OriGene (Rockville, MD, USA) and quantitative PCR analysis was performed (Sybr Green, Applied Biosystems, Foster City, CA, USA) using two independent oligo pairs for each BCL gene. The average level of mRNA in normal tissue samples (light bars) was used for each tissue type to determine the fold-change in expression of each sample (dark bars). The average of the fold-change between the two pairs of gene specific oligos was determined and the standard error of the mean is depicted. The horizontal line indicating a three-fold increase in relative expression serves as a reference. ( b ) Antibodies specific for each BCL family member were used for western blot analysis of whole cell lysates (1% CHAPS, 150 mM NaCl, 10 mM Hepes) from multiple leukemia/lymphoma cell lines.. An antibody against MYC was used to determine the levels of MYC protein, antibodies against beta-actin and tubulin were used as a loading control.

    Journal: Oncogene

    Article Title: MYC-induced myeloid leukemogenesis is accelerated by all six members of the anti-apoptotic BCL family

    doi: 10.1038/onc.2008.466

    Figure Lengend Snippet: BCL family members are expressed in human solid tumor samples and in leukemia/lymphoma cell lines. ( a ) Expression of BCL genes in human solid tumor samples. TissueScan oncology survey cDNA panels were obtained from OriGene (Rockville, MD, USA) and quantitative PCR analysis was performed (Sybr Green, Applied Biosystems, Foster City, CA, USA) using two independent oligo pairs for each BCL gene. The average level of mRNA in normal tissue samples (light bars) was used for each tissue type to determine the fold-change in expression of each sample (dark bars). The average of the fold-change between the two pairs of gene specific oligos was determined and the standard error of the mean is depicted. The horizontal line indicating a three-fold increase in relative expression serves as a reference. ( b ) Antibodies specific for each BCL family member were used for western blot analysis of whole cell lysates (1% CHAPS, 150 mM NaCl, 10 mM Hepes) from multiple leukemia/lymphoma cell lines.. An antibody against MYC was used to determine the levels of MYC protein, antibodies against beta-actin and tubulin were used as a loading control.

    Article Snippet: Using the “TissueScan oncology survey panel” from Origene, we performed quantitative real time PCR analysis (qPCR) on cDNA acquired from 60 different tissues and tumor samples for all six BCL genes, plus ACTIN as a control ( ).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, SYBR Green Assay, Western Blot