hspg2 libraries Search Results


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  • 99
    Thermo Fisher standard 96 well plates
    Standard 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    standard 96 well plates - by Bioz Stars, 2020-08
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    99
    Millipore perlecan gag peptide preparation
    Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested <t>perlecan</t> was applied onto a SAX-column and enriched for <t>GAG-glycopeptides.</t> The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.
    Perlecan Gag Peptide Preparation, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/perlecan gag peptide preparation/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    perlecan gag peptide preparation - by Bioz Stars, 2020-08
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    85
    Stratagene 129sv mouse genomic λ phage library
    Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested <t>perlecan</t> was applied onto a SAX-column and enriched for <t>GAG-glycopeptides.</t> The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.
    129sv Mouse Genomic λ Phage Library, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/129sv mouse genomic λ phage library/product/Stratagene
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    129sv mouse genomic λ phage library - by Bioz Stars, 2020-08
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    99
    TaKaRa long range pcr kit
    Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested <t>perlecan</t> was applied onto a SAX-column and enriched for <t>GAG-glycopeptides.</t> The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.
    Long Range Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range pcr kit/product/TaKaRa
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    long range pcr kit - by Bioz Stars, 2020-08
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    88
    TaKaRa long range polymerase chain reaction pcr
    Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested <t>perlecan</t> was applied onto a SAX-column and enriched for <t>GAG-glycopeptides.</t> The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.
    Long Range Polymerase Chain Reaction Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range polymerase chain reaction pcr/product/TaKaRa
    Average 88 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
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    92
    Stratagene poly a rna
    Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested <t>perlecan</t> was applied onto a SAX-column and enriched for <t>GAG-glycopeptides.</t> The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.
    Poly A Rna, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/poly a rna/product/Stratagene
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    poly a rna - by Bioz Stars, 2020-08
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    86
    Illumina Inc miseq 2x250bp run
    Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested <t>perlecan</t> was applied onto a SAX-column and enriched for <t>GAG-glycopeptides.</t> The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.
    Miseq 2x250bp Run, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miseq 2x250bp run/product/Illumina Inc
    Average 86 stars, based on 15 article reviews
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    miseq 2x250bp run - by Bioz Stars, 2020-08
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    99
    Thermo Fisher superscript first strand synthesis system
    Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested <t>perlecan</t> was applied onto a SAX-column and enriched for <t>GAG-glycopeptides.</t> The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.
    Superscript First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 42629 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript first strand synthesis system/product/Thermo Fisher
    Average 99 stars, based on 42629 article reviews
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    superscript first strand synthesis system - by Bioz Stars, 2020-08
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    Image Search Results


    Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested perlecan was applied onto a SAX-column and enriched for GAG-glycopeptides. The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.

    Journal: Scientific Reports

    Article Title: Site-specific identification of heparan and chondroitin sulfate glycosaminoglycans in hybrid proteoglycans

    doi: 10.1038/srep34537

    Figure Lengend Snippet: Site-specific analysis of heparan sulfate linkage region glycopeptides. ( a – d ) Trypsin-digested perlecan was applied onto a SAX-column and enriched for GAG-glycopeptides. The resulting preparation was digested with heparinase and analyzed with nLC-MS/MS. ( a ) An extracted-ion current chromatogram of MS2-spectra which have been filtered for the presence of the m/z 362.11 diagnostic ion, representing the dehydrated disaccharide ion [ΔHexAGlcNAc] + , revealed several 362-related peaks eluting at various positions ( b ) An HS-glycopeptide (1363.48; 4+) was identified at 43.6 min and the MS2-spectrum (NCE 20%) showed characteristic glycosidic fragments. ( c ) Enlarged view of the low mass range ( m/z 100–250) enabled the identification of several GlcNAc-derived oxonium ions. ( d ) MS2-fragmentation at higher energy (NCE 30%) provided several y-ions and one b-ion. The HS-glycopeptide is derived from the N-terminal domain of perlecan ( d , insert). All ions are [M + zH] + and their charges are shown as superscript when z > 1.

    Article Snippet: Perlecan GAG-peptide preparation Twenty microgram of the commercial perlecan sample, isolated from Engelbreth-Holm-Swarm mouse sarcoma, (H4777, Sigma-Aldrich) was trypsinized using an in-solution digestion protocol.

    Techniques: Mass Spectrometry, Diagnostic Assay, Derivative Assay

    Analysis of perlecan GAG-composition with SDS-PAGE. ( a ) Schematic illustration of perlecan. The proteoglycan may be substituted with GAGs at various positions, including three HS-chains at the N-terminal end as well as a CS-chain at the C-terminal end 18 . The number of disaccharides (n) and the degree of sulfation may vary at each GAG-position. ( b ) Trypsin-digested mouse perlecan was applied onto a SAX-column and enriched for GAG-glycopeptides (see Methods). The GAG-glycopeptides were eluted from the column with increasing salt concentrations (0.4 M, 0. 8 M and 1.6 M NaCl) and analyzed with SDS-PAGE / Alcian blue. Perlecan incubated with and without trypsin was loaded as control. ( c , d ) An identical set of SAX-enriched GAG-glycopeptides were treated with either chondroitinase ABC (CSase) ( c ) or heparinase (HSase) ( d ) prior to the SDS-PAGE / Alcian blue analysis.

    Journal: Scientific Reports

    Article Title: Site-specific identification of heparan and chondroitin sulfate glycosaminoglycans in hybrid proteoglycans

    doi: 10.1038/srep34537

    Figure Lengend Snippet: Analysis of perlecan GAG-composition with SDS-PAGE. ( a ) Schematic illustration of perlecan. The proteoglycan may be substituted with GAGs at various positions, including three HS-chains at the N-terminal end as well as a CS-chain at the C-terminal end 18 . The number of disaccharides (n) and the degree of sulfation may vary at each GAG-position. ( b ) Trypsin-digested mouse perlecan was applied onto a SAX-column and enriched for GAG-glycopeptides (see Methods). The GAG-glycopeptides were eluted from the column with increasing salt concentrations (0.4 M, 0. 8 M and 1.6 M NaCl) and analyzed with SDS-PAGE / Alcian blue. Perlecan incubated with and without trypsin was loaded as control. ( c , d ) An identical set of SAX-enriched GAG-glycopeptides were treated with either chondroitinase ABC (CSase) ( c ) or heparinase (HSase) ( d ) prior to the SDS-PAGE / Alcian blue analysis.

    Article Snippet: Perlecan GAG-peptide preparation Twenty microgram of the commercial perlecan sample, isolated from Engelbreth-Holm-Swarm mouse sarcoma, (H4777, Sigma-Aldrich) was trypsinized using an in-solution digestion protocol.

    Techniques: SDS Page, Incubation

    Biodistribution of 18 F-FP-OSP-1 and 18 F-FP-OSP-S (0.925 MBq per mouse) in 143B and UM-SCC1 tumor-bearing nude mice at 120 min after injection. Data are expressed as mean %ID/g ± SD (n=4 per group; **, P

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Phage Display Derived Peptides for Osteosarcoma Imaging

    doi: 10.1158/1078-0432.CCR-10-0968

    Figure Lengend Snippet: Biodistribution of 18 F-FP-OSP-1 and 18 F-FP-OSP-S (0.925 MBq per mouse) in 143B and UM-SCC1 tumor-bearing nude mice at 120 min after injection. Data are expressed as mean %ID/g ± SD (n=4 per group; **, P

    Article Snippet: To confirm that OSP-1 peptide binds to HSPG receptor, fixed 143B cells were blocked with 10% BSA for 30 min and then incubated with OSP-1 (10 μM) for 1 h at room temperature, followed by mouse anti-heparin/hepararan sulfate monoclonal antibody (1:300, Millipore, Temecula, CA) for 1 h at room temperature and then visualized with Cy3-conjugated donkey anti-mouse secondary antibody (1:300; Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques: Mouse Assay, Injection

    Scheme of the synthesis of 18 F-FP-OSP-1 (A) and 18 F-FP-OSP-S (B).

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Phage Display Derived Peptides for Osteosarcoma Imaging

    doi: 10.1158/1078-0432.CCR-10-0968

    Figure Lengend Snippet: Scheme of the synthesis of 18 F-FP-OSP-1 (A) and 18 F-FP-OSP-S (B).

    Article Snippet: To confirm that OSP-1 peptide binds to HSPG receptor, fixed 143B cells were blocked with 10% BSA for 30 min and then incubated with OSP-1 (10 μM) for 1 h at room temperature, followed by mouse anti-heparin/hepararan sulfate monoclonal antibody (1:300, Millipore, Temecula, CA) for 1 h at room temperature and then visualized with Cy3-conjugated donkey anti-mouse secondary antibody (1:300; Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques:

    Fluorescence staining to determine the binding affinity of OSP-1 and OSP-S in vitro and ex vivo . Scale bar equals 50 μm. A, fixed human osteosarcoma 143B cells and control 293T human embryonic kidney cells were stained using 100 nM FITC-OSP-1-phage.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Phage Display Derived Peptides for Osteosarcoma Imaging

    doi: 10.1158/1078-0432.CCR-10-0968

    Figure Lengend Snippet: Fluorescence staining to determine the binding affinity of OSP-1 and OSP-S in vitro and ex vivo . Scale bar equals 50 μm. A, fixed human osteosarcoma 143B cells and control 293T human embryonic kidney cells were stained using 100 nM FITC-OSP-1-phage.

    Article Snippet: To confirm that OSP-1 peptide binds to HSPG receptor, fixed 143B cells were blocked with 10% BSA for 30 min and then incubated with OSP-1 (10 μM) for 1 h at room temperature, followed by mouse anti-heparin/hepararan sulfate monoclonal antibody (1:300, Millipore, Temecula, CA) for 1 h at room temperature and then visualized with Cy3-conjugated donkey anti-mouse secondary antibody (1:300; Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques: Fluorescence, Staining, Binding Assay, In Vitro, Ex Vivo

    Immunofluorescence staining staining of heparan sulphate proteoglycan (HSPG) without and with OSP-1 blocking. Scale bar equals 50 μm. A, heparin/heparan sulfate monoclonal antibody staining of 143B and 293T cell lines. Magnification, 200 x. B,

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Phage Display Derived Peptides for Osteosarcoma Imaging

    doi: 10.1158/1078-0432.CCR-10-0968

    Figure Lengend Snippet: Immunofluorescence staining staining of heparan sulphate proteoglycan (HSPG) without and with OSP-1 blocking. Scale bar equals 50 μm. A, heparin/heparan sulfate monoclonal antibody staining of 143B and 293T cell lines. Magnification, 200 x. B,

    Article Snippet: To confirm that OSP-1 peptide binds to HSPG receptor, fixed 143B cells were blocked with 10% BSA for 30 min and then incubated with OSP-1 (10 μM) for 1 h at room temperature, followed by mouse anti-heparin/hepararan sulfate monoclonal antibody (1:300, Millipore, Temecula, CA) for 1 h at room temperature and then visualized with Cy3-conjugated donkey anti-mouse secondary antibody (1:300; Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques: Immunofluorescence, Staining, Blocking Assay

    A, Time dependent uptake of 18 F-FP-OSP-1 and 18 F-FP-OSP-S in 143B, UM-SCC1 and 293T cells (n=3, mean ± SD). B, Time dependent efflux of 18 F-FP-OSP-1 and 18 F-FP-OSP-S in 143B, UM-SCC1 and 293T cells (n=3, mean ± SD).

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Phage Display Derived Peptides for Osteosarcoma Imaging

    doi: 10.1158/1078-0432.CCR-10-0968

    Figure Lengend Snippet: A, Time dependent uptake of 18 F-FP-OSP-1 and 18 F-FP-OSP-S in 143B, UM-SCC1 and 293T cells (n=3, mean ± SD). B, Time dependent efflux of 18 F-FP-OSP-1 and 18 F-FP-OSP-S in 143B, UM-SCC1 and 293T cells (n=3, mean ± SD).

    Article Snippet: To confirm that OSP-1 peptide binds to HSPG receptor, fixed 143B cells were blocked with 10% BSA for 30 min and then incubated with OSP-1 (10 μM) for 1 h at room temperature, followed by mouse anti-heparin/hepararan sulfate monoclonal antibody (1:300, Millipore, Temecula, CA) for 1 h at room temperature and then visualized with Cy3-conjugated donkey anti-mouse secondary antibody (1:300; Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques:

    In vivo imaging of transplanted tumors by OSP-1 and OSP-S. A, Decay-corrected whole-body coronal microPET images of 143B and UM-SCC1 tumor-bearing mice at 30, 60 and 120 min after injection of 3.7MBq (100 μCi) of 18 F-FP-OSP-1 or 18 F-FP-OSP-S.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Phage Display Derived Peptides for Osteosarcoma Imaging

    doi: 10.1158/1078-0432.CCR-10-0968

    Figure Lengend Snippet: In vivo imaging of transplanted tumors by OSP-1 and OSP-S. A, Decay-corrected whole-body coronal microPET images of 143B and UM-SCC1 tumor-bearing mice at 30, 60 and 120 min after injection of 3.7MBq (100 μCi) of 18 F-FP-OSP-1 or 18 F-FP-OSP-S.

    Article Snippet: To confirm that OSP-1 peptide binds to HSPG receptor, fixed 143B cells were blocked with 10% BSA for 30 min and then incubated with OSP-1 (10 μM) for 1 h at room temperature, followed by mouse anti-heparin/hepararan sulfate monoclonal antibody (1:300, Millipore, Temecula, CA) for 1 h at room temperature and then visualized with Cy3-conjugated donkey anti-mouse secondary antibody (1:300; Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques: In Vivo Imaging, Mouse Assay, Injection