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    Cell Signaling Technology Inc hrp linked anti rabbit igg
    Interaction of AOPPs-HSA with RAGE: role on AOPPs-HSA-induced ROS generation. ( A ) Microplate wells were coated with 10 μ g/ml of AOPPs-HSA, CML-HSA, GA-HSA, or native HSA. After washing and blocking, the wells were incubated with indicated concentrations of sRAGE. The bound sRAGE was detected with a <t>HRP-conjugated</t> monoclonal α -RAGE <t>IgG.</t> ( B ) CML-HSA (10 μ g/ml) was immobilized onto the wells. sRAGE (4 μ g/ml) was pre-incubated with indicated concentrations of AOPPs-HSA, CML-HSA, GA-HSA, or native HSA, and then added to the wells. The binding of sRAGE with immobilized AGEs-HSA was detected as described in A . ( C ) HUVECs were pre-incubated with a polyclonal α -RAGE IgG, a nonimmune IgG, or excess sRAGE, and then stimulated with AOPPs-HSA. ROS generation was determined by measuring the DCF fluorescence. ( D ) AOPPs-HSA (200 μ g/ml) was pre-incubated with indicated concentrations of an α -AOPPs or a nonimmune IgG, and then interacted with HUVECs. ROS generation was determined as described above ( lower panel ). The α -AOPPs recognized AOPPs-HSA in Western blot ( upper panel, lane 1 ), but did not react with CML- ( lane 2 ), GA- ( lane 3 ), GC- ( lane 4 ), and RB-derived AGEs ( lane 5 ) or native HSA ( lane 6 ). ( E ) AOPPs-HSA was pre-incubated with indicated concentrations of 6D12 antibody and then interacted with HUVECs. ROS generation was measured as described above. ( F ) 6D12 antibody reacted with CML, GA- and GC-derived AGEs, and, to a lesser extent, with RB-derived protein. Data from three independent experiments are shown as mean ± SEM. ANOVA, p
    Hrp Linked Anti Rabbit Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 852 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp linked anti rabbit igg/product/Cell Signaling Technology Inc
    Average 99 stars, based on 852 article reviews
    Price from $9.99 to $1999.99
    hrp linked anti rabbit igg - by Bioz Stars, 2020-08
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    89
    Cell Signaling Technology Inc hrp linked anti rabbit igg antibody
    Interaction of AOPPs-HSA with RAGE: role on AOPPs-HSA-induced ROS generation. ( A ) Microplate wells were coated with 10 μ g/ml of AOPPs-HSA, CML-HSA, GA-HSA, or native HSA. After washing and blocking, the wells were incubated with indicated concentrations of sRAGE. The bound sRAGE was detected with a <t>HRP-conjugated</t> monoclonal α -RAGE <t>IgG.</t> ( B ) CML-HSA (10 μ g/ml) was immobilized onto the wells. sRAGE (4 μ g/ml) was pre-incubated with indicated concentrations of AOPPs-HSA, CML-HSA, GA-HSA, or native HSA, and then added to the wells. The binding of sRAGE with immobilized AGEs-HSA was detected as described in A . ( C ) HUVECs were pre-incubated with a polyclonal α -RAGE IgG, a nonimmune IgG, or excess sRAGE, and then stimulated with AOPPs-HSA. ROS generation was determined by measuring the DCF fluorescence. ( D ) AOPPs-HSA (200 μ g/ml) was pre-incubated with indicated concentrations of an α -AOPPs or a nonimmune IgG, and then interacted with HUVECs. ROS generation was determined as described above ( lower panel ). The α -AOPPs recognized AOPPs-HSA in Western blot ( upper panel, lane 1 ), but did not react with CML- ( lane 2 ), GA- ( lane 3 ), GC- ( lane 4 ), and RB-derived AGEs ( lane 5 ) or native HSA ( lane 6 ). ( E ) AOPPs-HSA was pre-incubated with indicated concentrations of 6D12 antibody and then interacted with HUVECs. ROS generation was measured as described above. ( F ) 6D12 antibody reacted with CML, GA- and GC-derived AGEs, and, to a lesser extent, with RB-derived protein. Data from three independent experiments are shown as mean ± SEM. ANOVA, p
    Hrp Linked Anti Rabbit Igg Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 89/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp linked anti rabbit igg antibody/product/Cell Signaling Technology Inc
    Average 89 stars, based on 177 article reviews
    Price from $9.99 to $1999.99
    hrp linked anti rabbit igg antibody - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

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    Interaction of AOPPs-HSA with RAGE: role on AOPPs-HSA-induced ROS generation. ( A ) Microplate wells were coated with 10 μ g/ml of AOPPs-HSA, CML-HSA, GA-HSA, or native HSA. After washing and blocking, the wells were incubated with indicated concentrations of sRAGE. The bound sRAGE was detected with a HRP-conjugated monoclonal α -RAGE IgG. ( B ) CML-HSA (10 μ g/ml) was immobilized onto the wells. sRAGE (4 μ g/ml) was pre-incubated with indicated concentrations of AOPPs-HSA, CML-HSA, GA-HSA, or native HSA, and then added to the wells. The binding of sRAGE with immobilized AGEs-HSA was detected as described in A . ( C ) HUVECs were pre-incubated with a polyclonal α -RAGE IgG, a nonimmune IgG, or excess sRAGE, and then stimulated with AOPPs-HSA. ROS generation was determined by measuring the DCF fluorescence. ( D ) AOPPs-HSA (200 μ g/ml) was pre-incubated with indicated concentrations of an α -AOPPs or a nonimmune IgG, and then interacted with HUVECs. ROS generation was determined as described above ( lower panel ). The α -AOPPs recognized AOPPs-HSA in Western blot ( upper panel, lane 1 ), but did not react with CML- ( lane 2 ), GA- ( lane 3 ), GC- ( lane 4 ), and RB-derived AGEs ( lane 5 ) or native HSA ( lane 6 ). ( E ) AOPPs-HSA was pre-incubated with indicated concentrations of 6D12 antibody and then interacted with HUVECs. ROS generation was measured as described above. ( F ) 6D12 antibody reacted with CML, GA- and GC-derived AGEs, and, to a lesser extent, with RB-derived protein. Data from three independent experiments are shown as mean ± SEM. ANOVA, p

    Journal: Antioxidants & Redox Signaling

    Article Title: Advanced Oxidation Protein Products Activate Vascular Endothelial Cells via a RAGE-Mediated Signaling Pathway

    doi: 10.1089/ars.2007.1999

    Figure Lengend Snippet: Interaction of AOPPs-HSA with RAGE: role on AOPPs-HSA-induced ROS generation. ( A ) Microplate wells were coated with 10 μ g/ml of AOPPs-HSA, CML-HSA, GA-HSA, or native HSA. After washing and blocking, the wells were incubated with indicated concentrations of sRAGE. The bound sRAGE was detected with a HRP-conjugated monoclonal α -RAGE IgG. ( B ) CML-HSA (10 μ g/ml) was immobilized onto the wells. sRAGE (4 μ g/ml) was pre-incubated with indicated concentrations of AOPPs-HSA, CML-HSA, GA-HSA, or native HSA, and then added to the wells. The binding of sRAGE with immobilized AGEs-HSA was detected as described in A . ( C ) HUVECs were pre-incubated with a polyclonal α -RAGE IgG, a nonimmune IgG, or excess sRAGE, and then stimulated with AOPPs-HSA. ROS generation was determined by measuring the DCF fluorescence. ( D ) AOPPs-HSA (200 μ g/ml) was pre-incubated with indicated concentrations of an α -AOPPs or a nonimmune IgG, and then interacted with HUVECs. ROS generation was determined as described above ( lower panel ). The α -AOPPs recognized AOPPs-HSA in Western blot ( upper panel, lane 1 ), but did not react with CML- ( lane 2 ), GA- ( lane 3 ), GC- ( lane 4 ), and RB-derived AGEs ( lane 5 ) or native HSA ( lane 6 ). ( E ) AOPPs-HSA was pre-incubated with indicated concentrations of 6D12 antibody and then interacted with HUVECs. ROS generation was measured as described above. ( F ) 6D12 antibody reacted with CML, GA- and GC-derived AGEs, and, to a lesser extent, with RB-derived protein. Data from three independent experiments are shown as mean ± SEM. ANOVA, p

    Article Snippet: Cell homogenates (40 μ g protein in each sample) were immunoblotted using the rabbit polyclonal antibodies against human pan- or phospho-ERK1/2, p38MAPK , and JNK1/2 as the primary antibodies, and a HRP-linked anti-rabbit IgG as the secondary antibody (all from Cell Signaling Technology).

    Techniques: Blocking Assay, Incubation, Binding Assay, Fluorescence, Western Blot, Derivative Assay