hrp-conjugated goat anti-mouse igg Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Bio-Rad hrp conjugated goat anti mouse igg
    LysGH15 induced specific <t>IgG</t> antibodies. Serum samples from immunized mice and control mice were collected every week for 10 weeks, and the titres ( A ) and concentrations of IgG, IgM and IgE isotypes ( B ) were measured using ELISA. ( C ) Western blotting assay of LysGH15. Mouse serum at 3 weeks post-immunization was used as the primary antibody (dilution 1:500), and <t>HRP-labeled</t> anti-mouse IgG antibody was used as the secondary antibody. The left lane shows the results for the LysGH15-injected mouse. The right lane shows the LysGH15 buffer-injected mouse. (n = 6 mice per group per experiment). *P
    Hrp Conjugated Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 741 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti mouse igg/product/Bio-Rad
    Average 99 stars, based on 741 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti mouse igg - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad hrp conjugated anti mouse igg
    LysGH15 induced specific <t>IgG</t> antibodies. Serum samples from immunized mice and control mice were collected every week for 10 weeks, and the titres ( A ) and concentrations of IgG, IgM and IgE isotypes ( B ) were measured using ELISA. ( C ) Western blotting assay of LysGH15. Mouse serum at 3 weeks post-immunization was used as the primary antibody (dilution 1:500), and <t>HRP-labeled</t> anti-mouse IgG antibody was used as the secondary antibody. The left lane shows the results for the LysGH15-injected mouse. The right lane shows the LysGH15 buffer-injected mouse. (n = 6 mice per group per experiment). *P
    Hrp Conjugated Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated anti mouse igg/product/Bio-Rad
    Average 99 stars, based on 394 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated anti mouse igg - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad hrp conjugated goat anti mouse
    Murine and rat <t>IGSF1-2</t> proteins do not traffic to the plasma membrane of transfected cells. A) HEK293 cells were transfected with expression plasmids for wild-type murine (M.) or rat (R.) IGSF1-2, murine BMP type IA receptor (BMPR1A), or empty vector (pcDNA4). Note, IGSF1-2 proteins were expressed with Myc/His tags at their C-termini, whereas BMPR1A had the Myc tag alone. Cell surface proteins were labeled with biotin prior to collection of whole cell lysates. Lysates were immunoprecipitated (IP) with Myc-beads and then subjected to SDS-PAGE and transferred to nitrocellulose membranes. Total proteins were immunoblotted with anti-Myc (bottom), whereas biotinylated proteins were identified with <t>streptavidin-HRP</t> (top). B) HEK293 cells were cultured on coverslips and transiently transfected with wild-type murine IGSF1-2-Myc/His. Cells were then fixed and subjected to immunofluorescence with a Myc antibody (green) under non-permeabilizing (top) and permeabilizing conditions (bottom). C) Cells were transfected as in panel B, premeabilized, and processed for double-label immunofluorescence with the Myc antibody (green) and an antibody against GRP-78/BiP (red). The overlay is shown in yellow. In B and C, nuclei were stained with DAPI. Images were captured by confocal microscopy. Scale bar, 10 μm.
    Hrp Conjugated Goat Anti Mouse, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti mouse/product/Bio-Rad
    Average 99 stars, based on 502 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti mouse - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad hrp conjugated goat antimouse
    Murine and rat <t>IGSF1-2</t> proteins do not traffic to the plasma membrane of transfected cells. A) HEK293 cells were transfected with expression plasmids for wild-type murine (M.) or rat (R.) IGSF1-2, murine BMP type IA receptor (BMPR1A), or empty vector (pcDNA4). Note, IGSF1-2 proteins were expressed with Myc/His tags at their C-termini, whereas BMPR1A had the Myc tag alone. Cell surface proteins were labeled with biotin prior to collection of whole cell lysates. Lysates were immunoprecipitated (IP) with Myc-beads and then subjected to SDS-PAGE and transferred to nitrocellulose membranes. Total proteins were immunoblotted with anti-Myc (bottom), whereas biotinylated proteins were identified with <t>streptavidin-HRP</t> (top). B) HEK293 cells were cultured on coverslips and transiently transfected with wild-type murine IGSF1-2-Myc/His. Cells were then fixed and subjected to immunofluorescence with a Myc antibody (green) under non-permeabilizing (top) and permeabilizing conditions (bottom). C) Cells were transfected as in panel B, premeabilized, and processed for double-label immunofluorescence with the Myc antibody (green) and an antibody against GRP-78/BiP (red). The overlay is shown in yellow. In B and C, nuclei were stained with DAPI. Images were captured by confocal microscopy. Scale bar, 10 μm.
    Hrp Conjugated Goat Antimouse, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat antimouse/product/Bio-Rad
    Average 99 stars, based on 128 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat antimouse - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    97
    R&D Systems hrp conjugated goat anti mouse igg
    Murine and rat <t>IGSF1-2</t> proteins do not traffic to the plasma membrane of transfected cells. A) HEK293 cells were transfected with expression plasmids for wild-type murine (M.) or rat (R.) IGSF1-2, murine BMP type IA receptor (BMPR1A), or empty vector (pcDNA4). Note, IGSF1-2 proteins were expressed with Myc/His tags at their C-termini, whereas BMPR1A had the Myc tag alone. Cell surface proteins were labeled with biotin prior to collection of whole cell lysates. Lysates were immunoprecipitated (IP) with Myc-beads and then subjected to SDS-PAGE and transferred to nitrocellulose membranes. Total proteins were immunoblotted with anti-Myc (bottom), whereas biotinylated proteins were identified with <t>streptavidin-HRP</t> (top). B) HEK293 cells were cultured on coverslips and transiently transfected with wild-type murine IGSF1-2-Myc/His. Cells were then fixed and subjected to immunofluorescence with a Myc antibody (green) under non-permeabilizing (top) and permeabilizing conditions (bottom). C) Cells were transfected as in panel B, premeabilized, and processed for double-label immunofluorescence with the Myc antibody (green) and an antibody against GRP-78/BiP (red). The overlay is shown in yellow. In B and C, nuclei were stained with DAPI. Images were captured by confocal microscopy. Scale bar, 10 μm.
    Hrp Conjugated Goat Anti Mouse Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti mouse igg/product/R&D Systems
    Average 97 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti mouse igg - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    97
    Bio-Rad goat anti mouse igg
    Murine and rat <t>IGSF1-2</t> proteins do not traffic to the plasma membrane of transfected cells. A) HEK293 cells were transfected with expression plasmids for wild-type murine (M.) or rat (R.) IGSF1-2, murine BMP type IA receptor (BMPR1A), or empty vector (pcDNA4). Note, IGSF1-2 proteins were expressed with Myc/His tags at their C-termini, whereas BMPR1A had the Myc tag alone. Cell surface proteins were labeled with biotin prior to collection of whole cell lysates. Lysates were immunoprecipitated (IP) with Myc-beads and then subjected to SDS-PAGE and transferred to nitrocellulose membranes. Total proteins were immunoblotted with anti-Myc (bottom), whereas biotinylated proteins were identified with <t>streptavidin-HRP</t> (top). B) HEK293 cells were cultured on coverslips and transiently transfected with wild-type murine IGSF1-2-Myc/His. Cells were then fixed and subjected to immunofluorescence with a Myc antibody (green) under non-permeabilizing (top) and permeabilizing conditions (bottom). C) Cells were transfected as in panel B, premeabilized, and processed for double-label immunofluorescence with the Myc antibody (green) and an antibody against GRP-78/BiP (red). The overlay is shown in yellow. In B and C, nuclei were stained with DAPI. Images were captured by confocal microscopy. Scale bar, 10 μm.
    Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg/product/Bio-Rad
    Average 97 stars, based on 1229 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    LysGH15 induced specific IgG antibodies. Serum samples from immunized mice and control mice were collected every week for 10 weeks, and the titres ( A ) and concentrations of IgG, IgM and IgE isotypes ( B ) were measured using ELISA. ( C ) Western blotting assay of LysGH15. Mouse serum at 3 weeks post-immunization was used as the primary antibody (dilution 1:500), and HRP-labeled anti-mouse IgG antibody was used as the secondary antibody. The left lane shows the results for the LysGH15-injected mouse. The right lane shows the LysGH15 buffer-injected mouse. (n = 6 mice per group per experiment). *P

    Journal: Scientific Reports

    Article Title: LysGH15 kills Staphylococcus aureus without being affected by the humoral immune response or inducing inflammation

    doi: 10.1038/srep29344

    Figure Lengend Snippet: LysGH15 induced specific IgG antibodies. Serum samples from immunized mice and control mice were collected every week for 10 weeks, and the titres ( A ) and concentrations of IgG, IgM and IgE isotypes ( B ) were measured using ELISA. ( C ) Western blotting assay of LysGH15. Mouse serum at 3 weeks post-immunization was used as the primary antibody (dilution 1:500), and HRP-labeled anti-mouse IgG antibody was used as the secondary antibody. The left lane shows the results for the LysGH15-injected mouse. The right lane shows the LysGH15 buffer-injected mouse. (n = 6 mice per group per experiment). *P

    Article Snippet: The membrane was subsequently incubated with HRP-conjugated goat anti-mouse IgG (Bio-Rad Laboratories, CA, US) diluted 1:5,000 in PBST.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Labeling, Injection

    Murine and rat IGSF1-2 proteins do not traffic to the plasma membrane of transfected cells. A) HEK293 cells were transfected with expression plasmids for wild-type murine (M.) or rat (R.) IGSF1-2, murine BMP type IA receptor (BMPR1A), or empty vector (pcDNA4). Note, IGSF1-2 proteins were expressed with Myc/His tags at their C-termini, whereas BMPR1A had the Myc tag alone. Cell surface proteins were labeled with biotin prior to collection of whole cell lysates. Lysates were immunoprecipitated (IP) with Myc-beads and then subjected to SDS-PAGE and transferred to nitrocellulose membranes. Total proteins were immunoblotted with anti-Myc (bottom), whereas biotinylated proteins were identified with streptavidin-HRP (top). B) HEK293 cells were cultured on coverslips and transiently transfected with wild-type murine IGSF1-2-Myc/His. Cells were then fixed and subjected to immunofluorescence with a Myc antibody (green) under non-permeabilizing (top) and permeabilizing conditions (bottom). C) Cells were transfected as in panel B, premeabilized, and processed for double-label immunofluorescence with the Myc antibody (green) and an antibody against GRP-78/BiP (red). The overlay is shown in yellow. In B and C, nuclei were stained with DAPI. Images were captured by confocal microscopy. Scale bar, 10 μm.

    Journal: PLoS ONE

    Article Title: The short mRNA isoform of the immunoglobulin superfamily, member 1 gene encodes an intracellular glycoprotein

    doi: 10.1371/journal.pone.0180731

    Figure Lengend Snippet: Murine and rat IGSF1-2 proteins do not traffic to the plasma membrane of transfected cells. A) HEK293 cells were transfected with expression plasmids for wild-type murine (M.) or rat (R.) IGSF1-2, murine BMP type IA receptor (BMPR1A), or empty vector (pcDNA4). Note, IGSF1-2 proteins were expressed with Myc/His tags at their C-termini, whereas BMPR1A had the Myc tag alone. Cell surface proteins were labeled with biotin prior to collection of whole cell lysates. Lysates were immunoprecipitated (IP) with Myc-beads and then subjected to SDS-PAGE and transferred to nitrocellulose membranes. Total proteins were immunoblotted with anti-Myc (bottom), whereas biotinylated proteins were identified with streptavidin-HRP (top). B) HEK293 cells were cultured on coverslips and transiently transfected with wild-type murine IGSF1-2-Myc/His. Cells were then fixed and subjected to immunofluorescence with a Myc antibody (green) under non-permeabilizing (top) and permeabilizing conditions (bottom). C) Cells were transfected as in panel B, premeabilized, and processed for double-label immunofluorescence with the Myc antibody (green) and an antibody against GRP-78/BiP (red). The overlay is shown in yellow. In B and C, nuclei were stained with DAPI. Images were captured by confocal microscopy. Scale bar, 10 μm.

    Article Snippet: The following antibodies were used at the indicated concentrations: monoclonal anti-mouse c-Myc (1:500, M5546, Sigma-Aldrich, St. Louis, MO), polyclonal rabbit anti-mouse IGSF1 (1:1000; previously described [ ]), and HRP-conjugated goat anti-mouse (1:5000, BioRad, Mississauga, Ontario).

    Techniques: Transfection, Expressing, IA, Plasmid Preparation, Labeling, Immunoprecipitation, SDS Page, Cell Culture, Immunofluorescence, Staining, Confocal Microscopy