Journal: Scientific Reports

Article Title: Globular-shaped variable lymphocyte receptors B antibody multimerized by a hydrophobic clustering in hagfish

doi: 10.1038/s41598-018-29197-w

Figure Lengend Snippet: Characterization of multimerized VLRB by hydrophobic clustering in hagfish plasma. ( A ) Total hagfish plasma proteins (3.5 μg/lane) were separated by 8% native-PAGE (left) or 12% SDS-PAGE (right) under non-reducing conditions, followed by Western blot analysis with 11G5. ( B ) Western blot analysis of VLRB in total hagfish plasma proteins treated with SDS (0, 0.003, 0.01, 0.03, 0.1 or 0.3%, v/v) at room temperature for 10 min. ( C ) Hagfish plasma samples were incubated with NP-40 (0.01, 0.03, or 0.1%, v/v), Triton X-100 (0.005, 0.015, or 0.03%, v/v), or SDS (0.003, 0.01, or 0.03%, v/v) at room temperature for 10 min. The samples were resolved by native-PAGE (8%), and VLRBs were detected with 11G5 followed by goat anti-mouse IgG-HRP.

Article Snippet: The blots were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Thermo Scientific), and the results were visualized using a SuperSignal West Pico Chemiluminescent Substrate kit (#34080, Thermo Scientific).

Techniques: Clear Native PAGE, SDS Page, Western Blot, Incubation