hrp conjugate Promega Search Results


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  • 99
    Promega anti rabbit igg h l hrp conjugate
    Anti Rabbit Igg H L Hrp Conjugate, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 984 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 984 article reviews
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    92
    Promega goat anti rabbit igg horse radish peroxidase hrp conjugate promega
    Immunological activity of the antibody preparations directed against human α-enolase (•―•) and β-enolase (о―о). ELISA plate wells were coated with 0.5 μg of purified α-, or β-enolase. The plates were washed, blocked with <t>TBST</t> and allowed to react with series of diluted, affinity chromatography purified, anti-human-α- and β-enolase antibody preparations. Goat <t>anti-rabbit-IgG-HRP</t> conjugate was used as the secondary antibody at 1:3000 dilution. Formation of the immunological complexes was tested by measuring HRP activity with o -phenylenediamine (OPD) as the HRP substrate. The progress of the HRP activity was monitored at 450 nm in a PerkinElmer microplate reader. Results were plotted and fitted into the linear regression equation. Slopes were calculated and compared. The data points are average of three experiments (n = 3) and average deviation from the mean for these data points was calculated at ± 0.015.
    Goat Anti Rabbit Igg Horse Radish Peroxidase Hrp Conjugate Promega, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg horse radish peroxidase hrp conjugate promega/product/Promega
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg horse radish peroxidase hrp conjugate promega - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    84
    Promega hrp conjugate promega
    Immunological activity of the antibody preparations directed against human α-enolase (•―•) and β-enolase (о―о). ELISA plate wells were coated with 0.5 μg of purified α-, or β-enolase. The plates were washed, blocked with <t>TBST</t> and allowed to react with series of diluted, affinity chromatography purified, anti-human-α- and β-enolase antibody preparations. Goat <t>anti-rabbit-IgG-HRP</t> conjugate was used as the secondary antibody at 1:3000 dilution. Formation of the immunological complexes was tested by measuring HRP activity with o -phenylenediamine (OPD) as the HRP substrate. The progress of the HRP activity was monitored at 450 nm in a PerkinElmer microplate reader. Results were plotted and fitted into the linear regression equation. Slopes were calculated and compared. The data points are average of three experiments (n = 3) and average deviation from the mean for these data points was calculated at ± 0.015.
    Hrp Conjugate Promega, supplied by Promega, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugate promega/product/Promega
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugate promega - by Bioz Stars, 2020-10
    84/100 stars
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    99
    Promega anti mouse igg h l hrp hrp horseradish peroxidase conjugate
    Immunological activity of the antibody preparations directed against human α-enolase (•―•) and β-enolase (о―о). ELISA plate wells were coated with 0.5 μg of purified α-, or β-enolase. The plates were washed, blocked with <t>TBST</t> and allowed to react with series of diluted, affinity chromatography purified, anti-human-α- and β-enolase antibody preparations. Goat <t>anti-rabbit-IgG-HRP</t> conjugate was used as the secondary antibody at 1:3000 dilution. Formation of the immunological complexes was tested by measuring HRP activity with o -phenylenediamine (OPD) as the HRP substrate. The progress of the HRP activity was monitored at 450 nm in a PerkinElmer microplate reader. Results were plotted and fitted into the linear regression equation. Slopes were calculated and compared. The data points are average of three experiments (n = 3) and average deviation from the mean for these data points was calculated at ± 0.015.
    Anti Mouse Igg H L Hrp Hrp Horseradish Peroxidase Conjugate, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse igg h l hrp hrp horseradish peroxidase conjugate/product/Promega
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti mouse igg h l hrp hrp horseradish peroxidase conjugate - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Immunological activity of the antibody preparations directed against human α-enolase (•―•) and β-enolase (о―о). ELISA plate wells were coated with 0.5 μg of purified α-, or β-enolase. The plates were washed, blocked with TBST and allowed to react with series of diluted, affinity chromatography purified, anti-human-α- and β-enolase antibody preparations. Goat anti-rabbit-IgG-HRP conjugate was used as the secondary antibody at 1:3000 dilution. Formation of the immunological complexes was tested by measuring HRP activity with o -phenylenediamine (OPD) as the HRP substrate. The progress of the HRP activity was monitored at 450 nm in a PerkinElmer microplate reader. Results were plotted and fitted into the linear regression equation. Slopes were calculated and compared. The data points are average of three experiments (n = 3) and average deviation from the mean for these data points was calculated at ± 0.015.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Experimental and bioinformatic approach to identifying antigenic epitopes in human α- and β-enolases

    doi: 10.1016/j.bbrep.2018.05.008

    Figure Lengend Snippet: Immunological activity of the antibody preparations directed against human α-enolase (•―•) and β-enolase (о―о). ELISA plate wells were coated with 0.5 μg of purified α-, or β-enolase. The plates were washed, blocked with TBST and allowed to react with series of diluted, affinity chromatography purified, anti-human-α- and β-enolase antibody preparations. Goat anti-rabbit-IgG-HRP conjugate was used as the secondary antibody at 1:3000 dilution. Formation of the immunological complexes was tested by measuring HRP activity with o -phenylenediamine (OPD) as the HRP substrate. The progress of the HRP activity was monitored at 450 nm in a PerkinElmer microplate reader. Results were plotted and fitted into the linear regression equation. Slopes were calculated and compared. The data points are average of three experiments (n = 3) and average deviation from the mean for these data points was calculated at ± 0.015.

    Article Snippet: Unoccupied protein binding sites on the polystyrene plate were blocked with 3% fat-free skimmed milk in TBS (20 mM Tris, 150 mM NaCl, pH 7.4) at 37 °C for 1 h. After washing with TBST, antibodies against human α-enolase or β-enolase were added in series of dilutions ranging from 0.003 μg/ml to 25 μg/ml and incubated at 37 °C for 2 h. The primary antibody was removed and after washing with TBST, the secondary antibody, goat anti-rabbit IgG-Horse Radish Peroxidase (HRP) conjugate (Promega) at 1:3000 dilution was added and incubated for 2 h. at 37 °C.

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Purification, Affinity Chromatography