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  • 98
    Millipore hptlc plate
    <t>HPTLC</t> analysis of gangliosides extracted from cultured Jurkat cells Jurkat cells were cultured alone or with 1–6 . (A) The HPTLC plate was stained for sialic acid using resorcinol. Jurkat cells produce a variety of gangliosides: GM3, GM2, <t>GM1a,</t> and GD1a. The pattern of gangliosides changed upon introduction of unnatural sialic acid precursors Ac 4 ManNDAz(2me) and Ac 4 ManNAz, but not other compounds. (B) The HPTLC plate was probed with CTxB-488, which recognizes ganglioside GM1a. Naturally occurring GM1a molecules are present in all ganglioside extracts. Additional species with CTxB-488 reactivity (s✯) appeared when cells were cultured with Ac 4 ManNDAz(2me), Ac 4 ManNDAz(3me), or Ac 4 ManNAz.
    Hptlc Plate, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Hichrom Limited hptlc plates
    <t>HPTLC</t> analysis of gangliosides extracted from cultured Jurkat cells Jurkat cells were cultured alone or with 1–6 . (A) The HPTLC plate was stained for sialic acid using resorcinol. Jurkat cells produce a variety of gangliosides: GM3, GM2, <t>GM1a,</t> and GD1a. The pattern of gangliosides changed upon introduction of unnatural sialic acid precursors Ac 4 ManNDAz(2me) and Ac 4 ManNAz, but not other compounds. (B) The HPTLC plate was probed with CTxB-488, which recognizes ganglioside GM1a. Naturally occurring GM1a molecules are present in all ganglioside extracts. Additional species with CTxB-488 reactivity (s✯) appeared when cells were cultured with Ac 4 ManNDAz(2me), Ac 4 ManNDAz(3me), or Ac 4 ManNAz.
    Hptlc Plates, supplied by Hichrom Limited, used in various techniques. Bioz Stars score: 91/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck & Co hptlc plates
    (A) Orcinol stain and <t>HPTLC</t> overlay of the glycolipids (upper phase) from midgut tissues of H. armigera with Cry1Ac and recombinant <t>LEC-8.</t> Two arrows indicated the glycolipids of longer chain oligosaccharides after staining with orcinol. It showed there was no difference for LEC-8 and Cry1Ac binding to the glycolipids. Sd, neutral glycolipid standard: 1, cerebroside (Gal-ceramide); 2, Lactosylceramide; 3, Globotriaosyl-ceramide; 4, globoside. Arrow head indicate the initial loading lane. (B) Pre-incubation of glycolipid with LEC-8 (L) reduced the binding of Cry1Ac (C) to glycolipid.
    Hptlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA hptlc plates
    F1394 increases free cholesterol/cholesterol ester ratio in LDs. <t>Huh7</t> cells were cultured for 24 h in the absence (Control) or presence of 10 μg/ml F1394 or 10 μg/ml 58-035 or treated with MβCD/cholesterol in LPDS (Chol) or MβCD/cholesterol plus 58-035 in LPDS (Chol + 58-035) as described in Materials and Methods . LD fractions were prepared and lipids analyzed by <t>HPTLC</t> as described in Materials and Methods . Results indicate the mean ± SD ( n = 3). * p = 0.05, ** p
    Hptlc Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    GE Healthcare hptlc plates
    F1394 increases free cholesterol/cholesterol ester ratio in LDs. <t>Huh7</t> cells were cultured for 24 h in the absence (Control) or presence of 10 μg/ml F1394 or 10 μg/ml 58-035 or treated with MβCD/cholesterol in LPDS (Chol) or MβCD/cholesterol plus 58-035 in LPDS (Chol + 58-035) as described in Materials and Methods . LD fractions were prepared and lipids analyzed by <t>HPTLC</t> as described in Materials and Methods . Results indicate the mean ± SD ( n = 3). * p = 0.05, ** p
    Hptlc Plates, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Camag hptlc plate
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Hptlc Plate, supplied by Camag, used in various techniques. Bioz Stars score: 90/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck & Co hptlc 60 plate
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Hptlc 60 Plate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck & Co multiple hptlc plates
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Multiple Hptlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck & Co 254 hptlc plates
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    254 Hptlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Merck KGaA silica60 hptlc plate
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Silica60 Hptlc Plate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck & Co si60 hptlc plate
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Si60 Hptlc Plate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck & Co precoated hptlc plates
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Precoated Hptlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Merck KGaA hptlc silicagel 60 plates
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Hptlc Silicagel 60 Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA hptlc rp 18 plates
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Hptlc Rp 18 Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck & Co 254 precoated hptlc plate
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    254 Precoated Hptlc Plate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA hptlc plates rp 8
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Hptlc Plates Rp 8, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Merck & Co glass backed hptlc plates
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Glass Backed Hptlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA 254 hptlc glass plates
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    254 Hptlc Glass Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MACHEREY NAGEL hptlc plates
    The <t>HPTLC</t> plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by <t>TLC–MS</t> interface and analyzed by ESI–MS (B) under positive mode.
    Hptlc Plates, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    HPTLC analysis of gangliosides extracted from cultured Jurkat cells Jurkat cells were cultured alone or with 1–6 . (A) The HPTLC plate was stained for sialic acid using resorcinol. Jurkat cells produce a variety of gangliosides: GM3, GM2, GM1a, and GD1a. The pattern of gangliosides changed upon introduction of unnatural sialic acid precursors Ac 4 ManNDAz(2me) and Ac 4 ManNAz, but not other compounds. (B) The HPTLC plate was probed with CTxB-488, which recognizes ganglioside GM1a. Naturally occurring GM1a molecules are present in all ganglioside extracts. Additional species with CTxB-488 reactivity (s✯) appeared when cells were cultured with Ac 4 ManNDAz(2me), Ac 4 ManNDAz(3me), or Ac 4 ManNAz.

    Journal: Bioconjugate chemistry

    Article Title: Metabolism of diazirine-modified N-acetylmannosamine analogs to photocrosslinking sialosides

    doi: 10.1021/bc2002117

    Figure Lengend Snippet: HPTLC analysis of gangliosides extracted from cultured Jurkat cells Jurkat cells were cultured alone or with 1–6 . (A) The HPTLC plate was stained for sialic acid using resorcinol. Jurkat cells produce a variety of gangliosides: GM3, GM2, GM1a, and GD1a. The pattern of gangliosides changed upon introduction of unnatural sialic acid precursors Ac 4 ManNDAz(2me) and Ac 4 ManNAz, but not other compounds. (B) The HPTLC plate was probed with CTxB-488, which recognizes ganglioside GM1a. Naturally occurring GM1a molecules are present in all ganglioside extracts. Additional species with CTxB-488 reactivity (s✯) appeared when cells were cultured with Ac 4 ManNDAz(2me), Ac 4 ManNDAz(3me), or Ac 4 ManNAz.

    Article Snippet: The HPTLC plate was then run in the chamber followed by drying under vacuum for 45 min. To assess the presence of GM1a, the HPTLC plate was immersed in 0.5% polyisobutylmethacrylate in hexanes (diluted from a stock solution of 2% in chloroform) for 2 min to fix the gangliosides to the TLC plate.

    Techniques: High Performance Thin Layer Chromatography, Cell Culture, Staining

    GD3S expression modifies GSL profiles in breast cancer cell lines ( A ) Upper-phase GSLs were separated on HPTLC plates with solvent system chloroform/ methanol/ 0.2% CaCl 2 in H 2 O (50:40:10, v/v/v), and visualized by orcinol spray (upper panel). GSL extract from each cell line was immune-stained with anti-GD2 (middle panel) or anti-GD3 (lower panel) mAb on TLC plate. Intact GSLs from MDA-MB231 ( B ), MDA-MB468 ( C ), and MCF7 ( D ) were permethylated and analyzed by MALDI-TOF MS. Parental untransfected wildtype control (WT), RFP/vector control, and GD3S-overexpressing transfectants are shown in parallel. Values shown are m/z of sodium adducted [M + Na] + molecular ions. Annotations of GSLs on spectra were assigned based on m/z values typical for ceramide moiety-associated fatty acyl heterogeneity. GSLs having the same glycan moiety but different fatty acyl components are bracketed as a group. Red triangles (▲): upregulated expression of GSLs. Blue inverted triangles (▼): downregulated expression of GSLs. GD2 and GD3 expression for each cell line was analyzed by flow cytometry using specific mAbs. Gray histograms: cells treated with isotype control. Bold black lines: fluorescence-positive cells. Percentages of number of cells having fluorescence values > 95% of isotype control value are shown.

    Journal: Oncotarget

    Article Title: Interaction of glycosphingolipids GD3 and GD2 with growth factor receptors maintains breast cancer stem cell phenotype

    doi: 10.18632/oncotarget.17665

    Figure Lengend Snippet: GD3S expression modifies GSL profiles in breast cancer cell lines ( A ) Upper-phase GSLs were separated on HPTLC plates with solvent system chloroform/ methanol/ 0.2% CaCl 2 in H 2 O (50:40:10, v/v/v), and visualized by orcinol spray (upper panel). GSL extract from each cell line was immune-stained with anti-GD2 (middle panel) or anti-GD3 (lower panel) mAb on TLC plate. Intact GSLs from MDA-MB231 ( B ), MDA-MB468 ( C ), and MCF7 ( D ) were permethylated and analyzed by MALDI-TOF MS. Parental untransfected wildtype control (WT), RFP/vector control, and GD3S-overexpressing transfectants are shown in parallel. Values shown are m/z of sodium adducted [M + Na] + molecular ions. Annotations of GSLs on spectra were assigned based on m/z values typical for ceramide moiety-associated fatty acyl heterogeneity. GSLs having the same glycan moiety but different fatty acyl components are bracketed as a group. Red triangles (▲): upregulated expression of GSLs. Blue inverted triangles (▼): downregulated expression of GSLs. GD2 and GD3 expression for each cell line was analyzed by flow cytometry using specific mAbs. Gray histograms: cells treated with isotype control. Bold black lines: fluorescence-positive cells. Percentages of number of cells having fluorescence values > 95% of isotype control value are shown.

    Article Snippet: GSLs were analyzed using HPTLC plates (EMD Bioscience) and developed in a solvent system of CM/0.5% aqueous CaCl2 (50:40:10).

    Techniques: Expressing, High Performance Thin Layer Chromatography, Staining, Thin Layer Chromatography, Multiple Displacement Amplification, Mass Spectrometry, Plasmid Preparation, Flow Cytometry, Cytometry, Fluorescence

    (A) Orcinol stain and HPTLC overlay of the glycolipids (upper phase) from midgut tissues of H. armigera with Cry1Ac and recombinant LEC-8. Two arrows indicated the glycolipids of longer chain oligosaccharides after staining with orcinol. It showed there was no difference for LEC-8 and Cry1Ac binding to the glycolipids. Sd, neutral glycolipid standard: 1, cerebroside (Gal-ceramide); 2, Lactosylceramide; 3, Globotriaosyl-ceramide; 4, globoside. Arrow head indicate the initial loading lane. (B) Pre-incubation of glycolipid with LEC-8 (L) reduced the binding of Cry1Ac (C) to glycolipid.

    Journal: Results in Immunology

    Article Title: Pre-feeding of a glycolipid binding protein LEC-8 from Caenorhabditis elegans revealed enhanced tolerance to Cry1Ac toxin in Helicoverpa armigera

    doi: 10.1016/j.rinim.2012.05.002

    Figure Lengend Snippet: (A) Orcinol stain and HPTLC overlay of the glycolipids (upper phase) from midgut tissues of H. armigera with Cry1Ac and recombinant LEC-8. Two arrows indicated the glycolipids of longer chain oligosaccharides after staining with orcinol. It showed there was no difference for LEC-8 and Cry1Ac binding to the glycolipids. Sd, neutral glycolipid standard: 1, cerebroside (Gal-ceramide); 2, Lactosylceramide; 3, Globotriaosyl-ceramide; 4, globoside. Arrow head indicate the initial loading lane. (B) Pre-incubation of glycolipid with LEC-8 (L) reduced the binding of Cry1Ac (C) to glycolipid.

    Article Snippet: To further test the effect of LEC-8 on the Cry1Ac binding to glycolipid, the HPTLC plate was pre incubated with LEC-8, then overlaid with Cry1Ac.

    Techniques: Staining, High Performance Thin Layer Chromatography, Recombinant, Binding Assay, Incubation

    F1394 increases free cholesterol/cholesterol ester ratio in LDs. Huh7 cells were cultured for 24 h in the absence (Control) or presence of 10 μg/ml F1394 or 10 μg/ml 58-035 or treated with MβCD/cholesterol in LPDS (Chol) or MβCD/cholesterol plus 58-035 in LPDS (Chol + 58-035) as described in Materials and Methods . LD fractions were prepared and lipids analyzed by HPTLC as described in Materials and Methods . Results indicate the mean ± SD ( n = 3). * p = 0.05, ** p

    Journal: Molecular Biology of the Cell

    Article Title: Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes

    doi: 10.1091/mbc.E15-10-0730

    Figure Lengend Snippet: F1394 increases free cholesterol/cholesterol ester ratio in LDs. Huh7 cells were cultured for 24 h in the absence (Control) or presence of 10 μg/ml F1394 or 10 μg/ml 58-035 or treated with MβCD/cholesterol in LPDS (Chol) or MβCD/cholesterol plus 58-035 in LPDS (Chol + 58-035) as described in Materials and Methods . LD fractions were prepared and lipids analyzed by HPTLC as described in Materials and Methods . Results indicate the mean ± SD ( n = 3). * p = 0.05, ** p

    Article Snippet: Thin-layer chromatography Lipids were extracted ( ) from Huh7 cells and applied to HPTLC plates (Merck, Darmstadt, Germany).

    Techniques: Cell Culture, High Performance Thin Layer Chromatography

    The HPTLC plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by TLC–MS interface and analyzed by ESI–MS (B) under positive mode.

    Journal: Frontiers in Microbiology

    Article Title: Production, Characterization, and Application of Bacillus licheniformis W16 Biosurfactant in Enhancing Oil Recovery

    doi: 10.3389/fmicb.2016.01853

    Figure Lengend Snippet: The HPTLC plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by TLC–MS interface and analyzed by ESI–MS (B) under positive mode.

    Article Snippet: Twenty microliter of biosurfactant (0.1 g l−1 ) sample was spotted onto a HPTLC plate (10 cm × 10 cm), Silica gel 60 – F254 (Merck, Germany).

    Techniques: High Performance Thin Layer Chromatography, Thin Layer Chromatography, Mass Spectrometry

    The HPTLC plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by TLC–MS interface and analyzed by ESI–MS (B) under positive mode.

    Journal: Frontiers in Microbiology

    Article Title: Production, Characterization, and Application of Bacillus licheniformis W16 Biosurfactant in Enhancing Oil Recovery

    doi: 10.3389/fmicb.2016.01853

    Figure Lengend Snippet: The HPTLC plate developed with four different solvent systems, showing separated biosurfactant under UV 254 nm (A) , and the mass spectra of two bands directly extracted by TLC–MS interface and analyzed by ESI–MS (B) under positive mode.

    Article Snippet: The HPTLC plates were evaluated by TLC visualizer (CAMAG) under direct Ultra Violet (UV 254 nm) light, and images were captured.

    Techniques: High Performance Thin Layer Chromatography, Thin Layer Chromatography, Mass Spectrometry