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  • 99
    Millipore pressure liquid chromatography hplc
    Pressure Liquid Chromatography Hplc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Varian Medical high pressure liquid chromatography hplc
    High Pressure Liquid Chromatography Hplc, supplied by Varian Medical, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Hitachi Ltd high pressure liquid chromatography hplc
    Representative <t>HPLC</t> chromatograms of phenolic <t>bioactive</t> compounds in walnut phenolic extract WPE. WPE was prepared from whole walnuts and its phenolic bioactive compounds, including gallic acid, (+)-catechin, chlorogenic acid, and ellagic, acid were detected by HPLC. WPE; walnut phenolic extract.
    High Pressure Liquid Chromatography Hplc, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shimadzu Corporation high pressure liquid chromatography hplc
    <t>HPLC</t> and UV spectrophotometric studies of the products of BacA and <t>BacB</t> enzymes. a , HPLC elution profiles of the BacA product with prephenate at different time points (1, 5, 10, and 60 min; 220 nm, 280 nm). b , HPLC elution profile of prephenate incubated with BacA alone, prephenate with BacB alone and prephenate with BacA and BacB. c , UV spectrophotometric time course measurement of the catalytic activity of BacA on prephenate. These spectra were recorded at intervals of 30 s. d , UV spectrophotometric time course measurement of the catalytic activity of BacB. These spectra were recorded at intervals of 30 s.
    High Pressure Liquid Chromatography Hplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shiseido high pressure liquid chromatography hplc
    <t>HPLC</t> and UV spectrophotometric studies of the products of BacA and <t>BacB</t> enzymes. a , HPLC elution profiles of the BacA product with prephenate at different time points (1, 5, 10, and 60 min; 220 nm, 280 nm). b , HPLC elution profile of prephenate incubated with BacA alone, prephenate with BacB alone and prephenate with BacA and BacB. c , UV spectrophotometric time course measurement of the catalytic activity of BacA on prephenate. These spectra were recorded at intervals of 30 s. d , UV spectrophotometric time course measurement of the catalytic activity of BacB. These spectra were recorded at intervals of 30 s.
    High Pressure Liquid Chromatography Hplc, supplied by Shiseido, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Waters Corporation high pressure liquid chromatography hplc
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies high pressure liquid chromatographer hplc
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatographer Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer high pressure liquid chromatography hplc
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography Hplc, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    scharlau high pressure liquid chromatography hplc
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography Hplc, supplied by scharlau, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high pressure liquid chromatography hplc
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography Hplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad high pressure liquid chromatography hplc
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography Hplc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation high pressure liquid chromatograph hplc n02104
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatograph Hplc N02104, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies 1100 high pressure liquid chromatography hplc
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    1100 High Pressure Liquid Chromatography Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific high pressure liquid chromatography hplc grade
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography Hplc Grade, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co high pressure liquid chromatography hplc solvents
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography Hplc Solvents, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Goebel Instrumentelle high pressure liquid chromatography hplc system
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography Hplc System, supplied by Goebel Instrumentelle, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Waters Corporation high pressure liquid chromatograph
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatograph, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore high pressure liquid chromatography
    The schematic diagram of the reaction and <t>HPLC</t> chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, <t>TSK-GEL</t> amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)
    High Pressure Liquid Chromatography, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies high pressure liquid chromatography hplc
    Analysis of products of <t>nPHA</t> granule hydrolysis and the enzyme activity of BdhA. (A) <t>HPLC</t> analysis of the product of nPHA granule hydrolysis by purified PhaZh1 after different reaction times. The curves from 1 to 4.5 h were translated to the vertical direction for convenient observation. The retention time of the 3-hydroxybutyrate (3HB) monomer was 13.7 min. (B) Turbidimetric assay (OD 650 ) of nPHA granule (isolated from the haloarchaeon H. mediterranei EPSΔphaZh1) hydrolysis by purified PhaZh1 (◼). The product (3HB) (●) released in the solution was identified via HPLC assay (A) using sodium 3-hydroxybutyrate (Sigma) as a standard. (C) SDS-PAGE analysis of BdhA purified from the haloarchaeon H. volcanii H1424 harboring the pTA05-bdhA plasmid. Lane 1, soluble crude extract; lane 2, His 6 -tagged BdhA purified with a Ni-agarose column; lane M, protein markers. (D) Properties of BdhA with different molarities of acetoacetate (0 to 2 mM) added to the reaction solution. The absorption at 340 nm of NADH produced in the solution was monitored spectrophotometrically.
    High Pressure Liquid Chromatography Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative HPLC chromatograms of phenolic bioactive compounds in walnut phenolic extract WPE. WPE was prepared from whole walnuts and its phenolic bioactive compounds, including gallic acid, (+)-catechin, chlorogenic acid, and ellagic, acid were detected by HPLC. WPE; walnut phenolic extract.

    Journal: Nutrients

    Article Title: Walnut Phenolic Extract and Its Bioactive Compounds Suppress Colon Cancer Cell Growth by Regulating Colon Cancer Stemness

    doi: 10.3390/nu8070439

    Figure Lengend Snippet: Representative HPLC chromatograms of phenolic bioactive compounds in walnut phenolic extract WPE. WPE was prepared from whole walnuts and its phenolic bioactive compounds, including gallic acid, (+)-catechin, chlorogenic acid, and ellagic, acid were detected by HPLC. WPE; walnut phenolic extract.

    Article Snippet: Analysis of Phenolic Bioactive Compounds by High-Pressure Liquid Chromatography (HPLC) The polyphenolic bioactive compounds present in the prepared WPE were analyzed by HPLC-photodiode array (PDA) analysis using a Hitachi Primaide 1430 HPLC system (Hitachi, Milford, MA, USA) with a Waters Symmetry® C18 column (3.9 × 150 mm, 5 μm inner diameter) (Waters, Milford, MA, USA).

    Techniques: High Performance Liquid Chromatography

    HPLC and UV spectrophotometric studies of the products of BacA and BacB enzymes. a , HPLC elution profiles of the BacA product with prephenate at different time points (1, 5, 10, and 60 min; 220 nm, 280 nm). b , HPLC elution profile of prephenate incubated with BacA alone, prephenate with BacB alone and prephenate with BacA and BacB. c , UV spectrophotometric time course measurement of the catalytic activity of BacA on prephenate. These spectra were recorded at intervals of 30 s. d , UV spectrophotometric time course measurement of the catalytic activity of BacB. These spectra were recorded at intervals of 30 s.

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Bacillus subtilis BacB in the Synthesis of Bacilysin *

    doi: 10.1074/jbc.M109.014522

    Figure Lengend Snippet: HPLC and UV spectrophotometric studies of the products of BacA and BacB enzymes. a , HPLC elution profiles of the BacA product with prephenate at different time points (1, 5, 10, and 60 min; 220 nm, 280 nm). b , HPLC elution profile of prephenate incubated with BacA alone, prephenate with BacB alone and prephenate with BacA and BacB. c , UV spectrophotometric time course measurement of the catalytic activity of BacA on prephenate. These spectra were recorded at intervals of 30 s. d , UV spectrophotometric time course measurement of the catalytic activity of BacB. These spectra were recorded at intervals of 30 s.

    Article Snippet: Characterization of the Reaction Products of BacA and BacB The reaction products of BacA and BacB were characterized using high pressure liquid chromatography (HPLC) using a Shimadzu SPD-20A instrument.

    Techniques: High Performance Liquid Chromatography, Incubation, Activity Assay

    The schematic diagram of the reaction and HPLC chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, TSK-GEL amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)

    Journal: AMB Express

    Article Title: Transglycosylation of gallic acid by using Leuconostoc glucansucrase and its characterization as a functional cosmetic agent

    doi: 10.1186/s13568-017-0523-x

    Figure Lengend Snippet: The schematic diagram of the reaction and HPLC chromatogram of gallic acid glucoside after preparative HPLC. Gallic acid standard (1 mg/mL) in ethanol ( a ), dextransucrase reaction digest for 6 h ( b ), purified gallic acid glucoside ( c ), MALDI-TOF MS spectrum of gallic acid glucoside ( d ), and the schematic diagram of the reaction ( e ). S gallic acid, R enzyme reaction mixture of gallic acid, and P final product. Column, TSK-GEL amide-80, 5 μm (Waters, Milford, MA, USA); mobile phase, acetonitrile/water = 80:20 (v/v); flow rate, 1.0 mL/min; room temperature; detection, RID-10A RI detector (Shimadzu)

    Article Snippet: The compound was purified by high-pressure liquid chromatography (HPLC) under the following conditions: column TSK-GEL amide-80, 5 μm (Waters, Milford, MA, USA); 80% (v/v) acetonitrile in water mobile phase; 1.0 mL/min flow rate; RID-10A RI detector (Shimadzu, Tokyo, Japan).

    Techniques: High Performance Liquid Chromatography, Purification, Mass Spectrometry, Flow Cytometry

    Analysis of products of nPHA granule hydrolysis and the enzyme activity of BdhA. (A) HPLC analysis of the product of nPHA granule hydrolysis by purified PhaZh1 after different reaction times. The curves from 1 to 4.5 h were translated to the vertical direction for convenient observation. The retention time of the 3-hydroxybutyrate (3HB) monomer was 13.7 min. (B) Turbidimetric assay (OD 650 ) of nPHA granule (isolated from the haloarchaeon H. mediterranei EPSΔphaZh1) hydrolysis by purified PhaZh1 (◼). The product (3HB) (●) released in the solution was identified via HPLC assay (A) using sodium 3-hydroxybutyrate (Sigma) as a standard. (C) SDS-PAGE analysis of BdhA purified from the haloarchaeon H. volcanii H1424 harboring the pTA05-bdhA plasmid. Lane 1, soluble crude extract; lane 2, His 6 -tagged BdhA purified with a Ni-agarose column; lane M, protein markers. (D) Properties of BdhA with different molarities of acetoacetate (0 to 2 mM) added to the reaction solution. The absorption at 340 nm of NADH produced in the solution was monitored spectrophotometrically.

    Journal: Applied and Environmental Microbiology

    Article Title: A Patatin-Like Protein Associated with the Polyhydroxyalkanoate (PHA) Granules of Haloferax mediterranei Acts as an Efficient Depolymerase in the Degradation of Native PHA

    doi: 10.1128/AEM.04269-14

    Figure Lengend Snippet: Analysis of products of nPHA granule hydrolysis and the enzyme activity of BdhA. (A) HPLC analysis of the product of nPHA granule hydrolysis by purified PhaZh1 after different reaction times. The curves from 1 to 4.5 h were translated to the vertical direction for convenient observation. The retention time of the 3-hydroxybutyrate (3HB) monomer was 13.7 min. (B) Turbidimetric assay (OD 650 ) of nPHA granule (isolated from the haloarchaeon H. mediterranei EPSΔphaZh1) hydrolysis by purified PhaZh1 (◼). The product (3HB) (●) released in the solution was identified via HPLC assay (A) using sodium 3-hydroxybutyrate (Sigma) as a standard. (C) SDS-PAGE analysis of BdhA purified from the haloarchaeon H. volcanii H1424 harboring the pTA05-bdhA plasmid. Lane 1, soluble crude extract; lane 2, His 6 -tagged BdhA purified with a Ni-agarose column; lane M, protein markers. (D) Properties of BdhA with different molarities of acetoacetate (0 to 2 mM) added to the reaction solution. The absorption at 340 nm of NADH produced in the solution was monitored spectrophotometrically.

    Article Snippet: The nPHA granule degradation product was identified via high-pressure liquid chromatography (HPLC) (HPLC-1220; Agilent, USA) as described previously ( ) using sodium 3-hydroxybutyrate (Sigma) as the standard.

    Techniques: Activity Assay, High Performance Liquid Chromatography, Purification, Turbidimetric Assay, Isolation, SDS Page, Plasmid Preparation, Produced