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  • 99
    Qiagen hotstartaq dna polymerase
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); <t>HotStarTaq</t> = HotStarTaq <t>DNA</t> Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Hotstartaq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 42693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq dna polymerase/product/Qiagen
    Average 99 stars, based on 42693 article reviews
    Price from $9.99 to $1999.99
    hotstartaq dna polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen hotstartaq polymerase
    Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with <t>HotStarTaq</t> ( open blue circles ) and KAPA HiFi DNA polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73
    Hotstartaq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2741 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerase/product/Qiagen
    Average 99 stars, based on 2741 article reviews
    Price from $9.99 to $1999.99
    hotstartaq polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen hotstarttaq dna polymerase
    Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with <t>HotStarTaq</t> ( open blue circles ) and KAPA HiFi DNA polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73
    Hotstarttaq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstarttaq dna polymerase/product/Qiagen
    Average 99 stars, based on 341 article reviews
    Price from $9.99 to $1999.99
    hotstarttaq dna polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen hotstartaq polymerase kit
    Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with <t>HotStarTaq</t> ( open blue circles ) and KAPA HiFi DNA polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73
    Hotstartaq Polymerase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerase kit/product/Qiagen
    Average 99 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    hotstartaq polymerase kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Journal: Scientific Reports

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    doi: 10.1038/s41598-019-40035-5

    Figure Lengend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Article Snippet: In order to find a balance, the performance of six different master mixes was tested (Fig. ), including the previously utilized HotStarTaq DNA Polymerase – and the upgraded AmpliTaq Gold 360 Master Mix , .

    Techniques: Polymerase Chain Reaction, Hot Start PCR

    Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with HotStarTaq ( open blue circles ) and KAPA HiFi DNA polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73

    Journal: BMC Microbiology

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems

    doi: 10.1186/s12866-017-0987-5

    Figure Lengend Snippet: Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with HotStarTaq ( open blue circles ) and KAPA HiFi DNA polymerases ( red open circles ). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R 2 ) of 0.73

    Article Snippet: The target-specific PCR mix comprised 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.4 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq Polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer and 1 ng of environmental DNA.

    Techniques: Sequencing, Amplification

    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Journal: PLoS ONE

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction

    doi: 10.1371/journal.pone.0073408

    Figure Lengend Snippet: Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Article Snippet: The AmpliTaq Gold DNA Polymerase in the AmpliTaq Gold 360 Master Mix, and the HotStarTaq Plus DNA Polymerase in the HotStarTaq Plus Master Mix (Qiagen) were recombinant forms modified from the Thermus aquaticus (Taq ) DNA polymerase.

    Techniques: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Marker