hotstartaq polymerase Search Results


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  • 95
    Qiagen hotstartaq dna polymerase pcr kit
    CXCL8 hypersecretion is not associated with differences in <t>DNA</t> methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were <t>PCR</t> amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.
    Hotstartaq Dna Polymerase Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq dna polymerase pcr kit/product/Qiagen
    Average 95 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    hotstartaq dna polymerase pcr kit - by Bioz Stars, 2020-02
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    95
    New England Biolabs hotstartaq dna polymerase
    CXCL8 hypersecretion is not associated with differences in <t>DNA</t> methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were <t>PCR</t> amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.
    Hotstartaq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq dna polymerase/product/New England Biolabs
    Average 95 stars, based on 4 article reviews
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    hotstartaq dna polymerase - by Bioz Stars, 2020-02
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    95
    Qiagen hotstartaq plus dna polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Plus Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1131 article reviews
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    79
    TaKaRa hotstartaq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher hotstartaq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerase/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
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    hotstartaq polymerase - by Bioz Stars, 2020-02
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    79
    Promega hotstartaq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerase/product/Promega
    Average 79 stars, based on 1 article reviews
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    hotstartaq polymerase - by Bioz Stars, 2020-02
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    85
    TaKaRa hotstartaq polymerase kit
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Polymerase Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerase kit/product/TaKaRa
    Average 85 stars, based on 24 article reviews
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    79
    DIATHEVA hotstartaq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Polymerase, supplied by DIATHEVA, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerase/product/DIATHEVA
    Average 79 stars, based on 1 article reviews
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    hotstartaq polymerase - by Bioz Stars, 2020-02
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    90
    Hain Lifescience hotstartaq dna polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Dna Polymerase, supplied by Hain Lifescience, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq dna polymerase/product/Hain Lifescience
    Average 90 stars, based on 8 article reviews
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    92
    TaKaRa hotstartaq dna polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq dna polymerase/product/TaKaRa
    Average 92 stars, based on 12 article reviews
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    hotstartaq dna polymerase - by Bioz Stars, 2020-02
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    90
    Thermo Fisher hotstartaq dna polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq dna polymerase/product/Thermo Fisher
    Average 90 stars, based on 52 article reviews
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    hotstartaq dna polymerase - by Bioz Stars, 2020-02
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    77
    Qiagen hotstartaq polymerse
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Polymerse, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerse/product/Qiagen
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    93
    Qiagen hotstarttaq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstarttaq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstarttaq polymerase/product/Qiagen
    Average 93 stars, based on 77 article reviews
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    77
    Qiagen hotstattaq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstattaq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstattaq polymerase/product/Qiagen
    Average 77 stars, based on 4 article reviews
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    hotstattaq polymerase - by Bioz Stars, 2020-02
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    81
    TaKaRa hotstarttaq dna polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstarttaq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 81/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Qiagen hotstartag polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartag Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Kapa Biosystems kapa fast hotstarttaq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Kapa Fast Hotstarttaq Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 77 stars, based on 5 article reviews
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    77
    Qiagen hotstartap polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartap Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartap polymerase/product/Qiagen
    Average 77 stars, based on 5 article reviews
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    hotstartap polymerase - by Bioz Stars, 2020-02
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    89
    Qiagen hotstartaq polymerase kit
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Polymerase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 89/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq polymerase kit/product/Qiagen
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    Millipore hotstartaq dna polymerase kit
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
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    Qiagen 1x hotstartaq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic <t>DNA,</t> at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
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    Qiagen hotstartaq master mix kit
    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and <t>HotStarTaq</t> was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.
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    Promega hotstartaq dna polymerase
    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and <t>HotStarTaq</t> was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.
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    tiangen biotech co hotstartaq dna polymerase
    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and <t>HotStarTaq</t> was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.
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    Biomol GmbH hotstartaq dna polymerase
    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and <t>HotStarTaq</t> was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.
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    Qiagen hotsartaq dna polymerase
    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and <t>HotStarTaq</t> was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.
    Hotsartaq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen hotstartaq plus master mix
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Plus Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen taq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
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    Qiagen hotstarttaq dna polymerase kit
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstarttaq Dna Polymerase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mgcl2
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Mgcl2, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 39277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taq polymerase
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
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    Image Search Results


    CXCL8 hypersecretion is not associated with differences in DNA methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were PCR amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET

    doi: 10.1152/ajplung.00021.2015

    Figure Lengend Snippet: CXCL8 hypersecretion is not associated with differences in DNA methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were PCR amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.

    Article Snippet: PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C.

    Techniques: DNA Methylation Assay, Isolation, Polymerase Chain Reaction, Amplification, Sequencing, Methylation

    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Journal: PLoS ONE

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction

    doi: 10.1371/journal.pone.0073408

    Figure Lengend Snippet: Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Article Snippet: The AmpliTaq Gold DNA Polymerase in the AmpliTaq Gold 360 Master Mix, and the HotStarTaq Plus DNA Polymerase in the HotStarTaq Plus Master Mix (Qiagen) were recombinant forms modified from the Thermus aquaticus (Taq ) DNA polymerase.

    Techniques: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Marker

    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.

    Journal: Experimental Animals

    Article Title: Zygosity Determination in Hairless Mice by PCR Based onHrhr Gene Analysis

    doi: 10.1538/expanim.62.267

    Figure Lengend Snippet: Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.

    Article Snippet: The difference between homozygous (Hrx /Hrx ) and wild-type (Hr /Hr ) genomes was determined using multiple PCRs with HotStarTaq (#203443, Qiagen; regions 1, 8–13) or KOD FX neo (KFX-201, TOYOBO, Osaka, Japan; regions 2–7) DNA polymerases.

    Techniques: Mouse Assay, Sequencing, Mutagenesis, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis

    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Journal: PLoS ONE

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction

    doi: 10.1371/journal.pone.0073408

    Figure Lengend Snippet: Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Article Snippet: Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR.

    Techniques: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Marker