hot-start taq polymerase Search Results


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  • 99
    New England Biolabs hotstart taq dna polymerase
    Hotstart Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher maxima hotstart taq dna polymerase
    Maxima Hotstart Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa hotstart taq polymerase
    Hotstart Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    New England Biolabs epimark hotstart taq dna polymerase
    Epimark Hotstart Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs longamp hotstart taq dna polymerase
    Longamp Hotstart Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher truestart hotstart taq dna polymerase
    Truestart Hotstart Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa hotstart la taq polymerase
    Hotstart La Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs hot start taq dna polymerase
    An overview of the write-store-read process. Data is encoded, with error correction, into <t>DNA</t> bases, which are synthesized into physical DNA molecules and stored. When a user wishes to read the data, the stored DNA is read by a DNA sequencer into bases and the decoding software corrects any errors retrieving the original data. ( a ) The logical flow from bits to bases to DNA and back. ( b ) A block diagram representation of the system hardware’s three modules: synthesis, storage, and sequencing. ( c ) A photograph showing the completed system. Highlighted are the storage vessel and the nanopore loading fixture. The majority of the remaining hardware is responsible for synthesis. ( d ) Overview of enzymatic preparation for DNA sequencing. An arbitrary 1 kilobase “extension segment” of DNA is PCR-amplified with <t>TAQ</t> polymerase, and a Bsa-I restriction site is added by the primer, leaving an A-tail and a TCGC sticky end after digestion. The extension segment is then T/A ligated to the standard Oxford Nanopore Technology (ONT) LSK-108 kit sequencing adapter, creating the “extended adapter,” which ensures that sufficient bases are read for successful base calling. For sequencing, the payload hairpin and extended adapter are ligated, forming a sequence-ready construct that does not require purification.
    Hot Start Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start taq dna polymerase/product/New England Biolabs
    Average 99 stars, based on 158 article reviews
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    93
    Thermo Fisher hot start taq dna polymerase
    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start <t>Taq</t> <t>DNA</t> polymerase at same dilutions; lane M 100 bp marker
    Hot Start Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SibEnzyme hot start taq dna polymerase
    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start <t>Taq</t> <t>DNA</t> polymerase at same dilutions; lane M 100 bp marker
    Hot Start Taq Dna Polymerase, supplied by SibEnzyme, used in various techniques. Bioz Stars score: 93/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega hot start taq polymerase
    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start <t>Taq</t> <t>DNA</t> polymerase at same dilutions; lane M 100 bp marker
    Hot Start Taq Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bangalore Genei hot start taq polymerase
    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start <t>Taq</t> <t>DNA</t> polymerase at same dilutions; lane M 100 bp marker
    Hot Start Taq Polymerase, supplied by Bangalore Genei, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    TaKaRa hot start taq dna polymerase
    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start <t>Taq</t> <t>DNA</t> polymerase at same dilutions; lane M 100 bp marker
    Hot Start Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa hot start hs taq polymerase
    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start <t>Taq</t> <t>DNA</t> polymerase at same dilutions; lane M 100 bp marker
    Hot Start Hs Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    An overview of the write-store-read process. Data is encoded, with error correction, into DNA bases, which are synthesized into physical DNA molecules and stored. When a user wishes to read the data, the stored DNA is read by a DNA sequencer into bases and the decoding software corrects any errors retrieving the original data. ( a ) The logical flow from bits to bases to DNA and back. ( b ) A block diagram representation of the system hardware’s three modules: synthesis, storage, and sequencing. ( c ) A photograph showing the completed system. Highlighted are the storage vessel and the nanopore loading fixture. The majority of the remaining hardware is responsible for synthesis. ( d ) Overview of enzymatic preparation for DNA sequencing. An arbitrary 1 kilobase “extension segment” of DNA is PCR-amplified with TAQ polymerase, and a Bsa-I restriction site is added by the primer, leaving an A-tail and a TCGC sticky end after digestion. The extension segment is then T/A ligated to the standard Oxford Nanopore Technology (ONT) LSK-108 kit sequencing adapter, creating the “extended adapter,” which ensures that sufficient bases are read for successful base calling. For sequencing, the payload hairpin and extended adapter are ligated, forming a sequence-ready construct that does not require purification.

    Journal: Scientific Reports

    Article Title: Demonstration of End-to-End Automation of DNA Data Storage

    doi: 10.1038/s41598-019-41228-8

    Figure Lengend Snippet: An overview of the write-store-read process. Data is encoded, with error correction, into DNA bases, which are synthesized into physical DNA molecules and stored. When a user wishes to read the data, the stored DNA is read by a DNA sequencer into bases and the decoding software corrects any errors retrieving the original data. ( a ) The logical flow from bits to bases to DNA and back. ( b ) A block diagram representation of the system hardware’s three modules: synthesis, storage, and sequencing. ( c ) A photograph showing the completed system. Highlighted are the storage vessel and the nanopore loading fixture. The majority of the remaining hardware is responsible for synthesis. ( d ) Overview of enzymatic preparation for DNA sequencing. An arbitrary 1 kilobase “extension segment” of DNA is PCR-amplified with TAQ polymerase, and a Bsa-I restriction site is added by the primer, leaving an A-tail and a TCGC sticky end after digestion. The extension segment is then T/A ligated to the standard Oxford Nanopore Technology (ONT) LSK-108 kit sequencing adapter, creating the “extended adapter,” which ensures that sufficient bases are read for successful base calling. For sequencing, the payload hairpin and extended adapter are ligated, forming a sequence-ready construct that does not require purification.

    Article Snippet: Sequencing preparation The extended adapter was constructed from a 1 kilobase fragment that was PCR-amplified from the lambda genome using hot start TAQ DNA polymerase (NEB M0496) with a Bsa-I restriction site added by the forward primer.

    Techniques: Synthesized, Software, Flow Cytometry, Blocking Assay, Sequencing, DNA Sequencing, Polymerase Chain Reaction, Amplification, Construct, Purification

    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start Taq DNA polymerase at same dilutions; lane M 100 bp marker

    Journal: Indian Journal of Microbiology

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid

    doi: 10.1007/s12088-013-0431-y

    Figure Lengend Snippet: Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start Taq DNA polymerase at same dilutions; lane M 100 bp marker

    Article Snippet: Same reaction composition was carried out with Hot Start Taq DNA polymerase (Fermentas, Vilnius, Lithuania).

    Techniques: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Amplification, Multiplexing, Marker