hot-start taq polymerase Search Results


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  • 90
    Thermo Fisher truestart hot start taq dna polymerase
    Truestart Hot Start Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truestart hot start taq dna polymerase/product/Thermo Fisher
    Average 90 stars, based on 65 article reviews
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    97
    Thermo Fisher maxma hot start taq dna polymerase
    Maxma Hot Start Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxma hot start taq dna polymerase/product/Thermo Fisher
    Average 97 stars, based on 30 article reviews
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    99
    New England Biolabs hot start taq dna polymerase
    An overview of the write-store-read process. Data is encoded, with error correction, into <t>DNA</t> bases, which are synthesized into physical DNA molecules and stored. When a user wishes to read the data, the stored DNA is read by a DNA sequencer into bases and the decoding software corrects any errors retrieving the original data. ( a ) The logical flow from bits to bases to DNA and back. ( b ) A block diagram representation of the system hardware’s three modules: synthesis, storage, and sequencing. ( c ) A photograph showing the completed system. Highlighted are the storage vessel and the nanopore loading fixture. The majority of the remaining hardware is responsible for synthesis. ( d ) Overview of enzymatic preparation for DNA sequencing. An arbitrary 1 kilobase “extension segment” of DNA is PCR-amplified with <t>TAQ</t> polymerase, and a Bsa-I restriction site is added by the primer, leaving an A-tail and a TCGC sticky end after digestion. The extension segment is then T/A ligated to the standard Oxford Nanopore Technology (ONT) LSK-108 kit sequencing adapter, creating the “extended adapter,” which ensures that sufficient bases are read for successful base calling. For sequencing, the payload hairpin and extended adapter are ligated, forming a sequence-ready construct that does not require purification.
    Hot Start Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start taq dna polymerase/product/New England Biolabs
    Average 99 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    hot start taq dna polymerase - by Bioz Stars, 2019-12
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    99
    Thermo Fisher maxima hot start taq dna polymerase
    Neq SSB-like dsDNA and mRNA binding properties. A Binding to 2.5 pmol of 100 bp PCR product. Lanes 1–6 contain 0, 10, 20, 40, 80 and 160 pmoles of Neq SSB-like, respectively. B Binding to 0.132 pmol of Escherichia coli genomic <t>DNA.</t> Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. C Binding to 0.2 pmol of pDONR201 plasmid DNA (4470 bp). Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. D Binding to 0.1 pmol of pDONR201 plasmid DNA + 0.05 pmol of linearized pDONR201 plasmid DNA. Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. E Control binding reaction with 0.2 pmol of pDONR201 plasmid DNA. Lanes 1–4 contain 0, 10, 20 and 40 pmoles of <t>Taq</t> SSB, respectively. F Binding to 980 ng of mRNA. Lanes 1–5 contain 0, 10, 20, 40, 80 pmoles of Neq SSB-like, respectively.
    Maxima Hot Start Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxima hot start taq dna polymerase/product/Thermo Fisher
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    94
    TaKaRa ex taq dna polymerase hot start version
    Sequencing electropherogram of (CA)7 . PCR of the mtDNA D-loop (CA)7 was performed using EX <t>Taq</t> <t>DNA</t> polymerase and F7/R5 primer pairs (A) or F5/R5 primer pairs (B). The sequencing electropherogram using the F5 primer is the same as (data not shown). (C) PCR was performed using a different proofreading DNA polymerase KOD and F7/R5 primer pairs. (D) PCR of genomic (CA)7 of the NAALAD2 gene was performed using EX Taq DNA polymerase. An arrow indicates a representative position used to evaluate a variant allele. PCR, polymerase chain reaction; mtDNA, mitochondrial DNA; NB, normal breast epithelia; NLN, normal lymph node; BC, breast cancer; NL16, normal liver; F, forward; R, reverse. NL16 is a different patient with a (CA)4 repeat.
    Ex Taq Dna Polymerase Hot Start Version, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Tonbo Biosciences hot start taq dna polymerase master mix
    Sequencing electropherogram of (CA)7 . PCR of the mtDNA D-loop (CA)7 was performed using EX <t>Taq</t> <t>DNA</t> polymerase and F7/R5 primer pairs (A) or F5/R5 primer pairs (B). The sequencing electropherogram using the F5 primer is the same as (data not shown). (C) PCR was performed using a different proofreading DNA polymerase KOD and F7/R5 primer pairs. (D) PCR of genomic (CA)7 of the NAALAD2 gene was performed using EX Taq DNA polymerase. An arrow indicates a representative position used to evaluate a variant allele. PCR, polymerase chain reaction; mtDNA, mitochondrial DNA; NB, normal breast epithelia; NLN, normal lymph node; BC, breast cancer; NL16, normal liver; F, forward; R, reverse. NL16 is a different patient with a (CA)4 repeat.
    Hot Start Taq Dna Polymerase Master Mix, supplied by Tonbo Biosciences, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start taq dna polymerase master mix/product/Tonbo Biosciences
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    81
    Qiagen hot start taq polymerase pcr master mix 2×
    Sequencing electropherogram of (CA)7 . PCR of the mtDNA D-loop (CA)7 was performed using EX <t>Taq</t> <t>DNA</t> polymerase and F7/R5 primer pairs (A) or F5/R5 primer pairs (B). The sequencing electropherogram using the F5 primer is the same as (data not shown). (C) PCR was performed using a different proofreading DNA polymerase KOD and F7/R5 primer pairs. (D) PCR of genomic (CA)7 of the NAALAD2 gene was performed using EX Taq DNA polymerase. An arrow indicates a representative position used to evaluate a variant allele. PCR, polymerase chain reaction; mtDNA, mitochondrial DNA; NB, normal breast epithelia; NLN, normal lymph node; BC, breast cancer; NL16, normal liver; F, forward; R, reverse. NL16 is a different patient with a (CA)4 repeat.
    Hot Start Taq Polymerase Pcr Master Mix 2×, supplied by Qiagen, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start taq polymerase pcr master mix 2×/product/Qiagen
    Average 81 stars, based on 4 article reviews
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    75
    Kapa Biosystems fast hot start taq dna polymerase
    HotStart and <t>low-DNA</t> <t>Taq</t> DNA polymerases are not sufficiently pure for sensitive and specific broad-range amplification of bacterial DNA. The genomic DNA (100 fg) of S. aureus was amplified by HotStart or low-DNA Taq DNA polymerases (Taq #1: Hot Start Taq DNA polymerase, Protech Inc.; Taq #2: Fast Hot Start Taq DNA polymerase, KAPA Biosystems; Taq #3: Taq DNA polymerase, TakaRa Inc.; Taq #4: ULTRATOOLS Taq DNA polymerase, Biotools Inc.) using the primer set p201 and p1370 (lanes 1, 3, 5, and 7). Significant amount of PCR product was present in the no template control reactions (lanes 2, 4, 6, and 8).
    Fast Hot Start Taq Dna Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast hot start taq dna polymerase/product/Kapa Biosystems
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    Image Search Results


    An overview of the write-store-read process. Data is encoded, with error correction, into DNA bases, which are synthesized into physical DNA molecules and stored. When a user wishes to read the data, the stored DNA is read by a DNA sequencer into bases and the decoding software corrects any errors retrieving the original data. ( a ) The logical flow from bits to bases to DNA and back. ( b ) A block diagram representation of the system hardware’s three modules: synthesis, storage, and sequencing. ( c ) A photograph showing the completed system. Highlighted are the storage vessel and the nanopore loading fixture. The majority of the remaining hardware is responsible for synthesis. ( d ) Overview of enzymatic preparation for DNA sequencing. An arbitrary 1 kilobase “extension segment” of DNA is PCR-amplified with TAQ polymerase, and a Bsa-I restriction site is added by the primer, leaving an A-tail and a TCGC sticky end after digestion. The extension segment is then T/A ligated to the standard Oxford Nanopore Technology (ONT) LSK-108 kit sequencing adapter, creating the “extended adapter,” which ensures that sufficient bases are read for successful base calling. For sequencing, the payload hairpin and extended adapter are ligated, forming a sequence-ready construct that does not require purification.

    Journal: Scientific Reports

    Article Title: Demonstration of End-to-End Automation of DNA Data Storage

    doi: 10.1038/s41598-019-41228-8

    Figure Lengend Snippet: An overview of the write-store-read process. Data is encoded, with error correction, into DNA bases, which are synthesized into physical DNA molecules and stored. When a user wishes to read the data, the stored DNA is read by a DNA sequencer into bases and the decoding software corrects any errors retrieving the original data. ( a ) The logical flow from bits to bases to DNA and back. ( b ) A block diagram representation of the system hardware’s three modules: synthesis, storage, and sequencing. ( c ) A photograph showing the completed system. Highlighted are the storage vessel and the nanopore loading fixture. The majority of the remaining hardware is responsible for synthesis. ( d ) Overview of enzymatic preparation for DNA sequencing. An arbitrary 1 kilobase “extension segment” of DNA is PCR-amplified with TAQ polymerase, and a Bsa-I restriction site is added by the primer, leaving an A-tail and a TCGC sticky end after digestion. The extension segment is then T/A ligated to the standard Oxford Nanopore Technology (ONT) LSK-108 kit sequencing adapter, creating the “extended adapter,” which ensures that sufficient bases are read for successful base calling. For sequencing, the payload hairpin and extended adapter are ligated, forming a sequence-ready construct that does not require purification.

    Article Snippet: The extended adapter was constructed from a 1 kilobase fragment that was PCR-amplified from the lambda genome using hot start TAQ DNA polymerase (NEB M0496) with a Bsa-I restriction site added by the forward primer.

    Techniques: Synthesized, Software, Flow Cytometry, Blocking Assay, Sequencing, DNA Sequencing, Polymerase Chain Reaction, Amplification, Construct, Purification

    Neq SSB-like dsDNA and mRNA binding properties. A Binding to 2.5 pmol of 100 bp PCR product. Lanes 1–6 contain 0, 10, 20, 40, 80 and 160 pmoles of Neq SSB-like, respectively. B Binding to 0.132 pmol of Escherichia coli genomic DNA. Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. C Binding to 0.2 pmol of pDONR201 plasmid DNA (4470 bp). Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. D Binding to 0.1 pmol of pDONR201 plasmid DNA + 0.05 pmol of linearized pDONR201 plasmid DNA. Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. E Control binding reaction with 0.2 pmol of pDONR201 plasmid DNA. Lanes 1–4 contain 0, 10, 20 and 40 pmoles of Taq SSB, respectively. F Binding to 980 ng of mRNA. Lanes 1–5 contain 0, 10, 20, 40, 80 pmoles of Neq SSB-like, respectively.

    Journal: PLoS ONE

    Article Title: Characterization of a Single-Stranded DNA-Binding-Like Protein from Nanoarchaeum equitans—A Nucleic Acid Binding Protein with Broad Substrate Specificity

    doi: 10.1371/journal.pone.0126563

    Figure Lengend Snippet: Neq SSB-like dsDNA and mRNA binding properties. A Binding to 2.5 pmol of 100 bp PCR product. Lanes 1–6 contain 0, 10, 20, 40, 80 and 160 pmoles of Neq SSB-like, respectively. B Binding to 0.132 pmol of Escherichia coli genomic DNA. Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. C Binding to 0.2 pmol of pDONR201 plasmid DNA (4470 bp). Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. D Binding to 0.1 pmol of pDONR201 plasmid DNA + 0.05 pmol of linearized pDONR201 plasmid DNA. Lanes 1–7 contain 0, 10, 20, 40, 80, 160 and 320 pmoles of Neq SSB-like, respectively. E Control binding reaction with 0.2 pmol of pDONR201 plasmid DNA. Lanes 1–4 contain 0, 10, 20 and 40 pmoles of Taq SSB, respectively. F Binding to 980 ng of mRNA. Lanes 1–5 contain 0, 10, 20, 40, 80 pmoles of Neq SSB-like, respectively.

    Article Snippet: The PCR reaction solution consisted of 0.2 μg of Nanoarchaeum equitans Kin4-M genome DNA, 1 μl (10 μM) of each primer, 2.5 μl (10 mM) dNTPs, 2 μl (25 mM) MgCl2 , 2.5 μl of 10 x Hot Start Buffer (200 mM Tris-HCl pH 8.3, 200 mM KCl, 50 mM (NH4 )2 SO4 ), and 2 U of Maxima Hot Start Taq DNA Polymerase (Fermentas, Lithuania).

    Techniques: Binding Assay, Polymerase Chain Reaction, Plasmid Preparation

    Comparison of different concentrations of Taq -polymerase in PCR with Amplifluor-like SNP markers for ‘trouble-shooting’ of allele discrimination. The identicial PCR protocol was applied with the same DNA samples from a wheat collection from Kazakhstan and the same marker W58, using 0.5 units ( a ) or 0.1 units ( b ) of Maxima Hot-start Taq -polymerase (ThermoFisher, USA). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Journal: BMC Plant Biology

    Article Title: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping

    doi: 10.1186/s12870-017-1197-x

    Figure Lengend Snippet: Comparison of different concentrations of Taq -polymerase in PCR with Amplifluor-like SNP markers for ‘trouble-shooting’ of allele discrimination. The identicial PCR protocol was applied with the same DNA samples from a wheat collection from Kazakhstan and the same marker W58, using 0.5 units ( a ) or 0.1 units ( b ) of Maxima Hot-start Taq -polymerase (ThermoFisher, USA). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Article Snippet: The PCR cocktail contained 2 x Master-mix with the following reagents in final concentrations: 1 x PCR Buffer, 1.8 mM MgCl2 , 0.2 mM each of dNTPs, 0.25 μM each fluorescent label probe, 0.15 μM of each forward primer, 0.78 μM of reverse primer and 0.5 units of Taq DNA polymerase (GenLab, Astana, Kazakhstan) or 0.1 units of Maxima Hot-Start Taq -polymerase (ThermoFisher, USA).

    Techniques: Polymerase Chain Reaction, Marker, Amplification, Fluorescence, Real-time Polymerase Chain Reaction

    Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electropho resis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana (reviewed in [ 38 ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Journal: Epigenetics & Chromatin

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia

    doi: 10.1186/s13072-018-0201-5

    Figure Lengend Snippet: Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electropho resis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana (reviewed in [ 38 ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Article Snippet: End-point PCR was carried out on a VWR Doppio PCR device using Maxima Hot Start Taq Polymerase (ThermoFisher) or Pfu DNA Polymerase (ThermoFisher).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Amplification, Sequencing, Purification

    Sequencing electropherogram of (CA)7 . PCR of the mtDNA D-loop (CA)7 was performed using EX Taq DNA polymerase and F7/R5 primer pairs (A) or F5/R5 primer pairs (B). The sequencing electropherogram using the F5 primer is the same as (data not shown). (C) PCR was performed using a different proofreading DNA polymerase KOD and F7/R5 primer pairs. (D) PCR of genomic (CA)7 of the NAALAD2 gene was performed using EX Taq DNA polymerase. An arrow indicates a representative position used to evaluate a variant allele. PCR, polymerase chain reaction; mtDNA, mitochondrial DNA; NB, normal breast epithelia; NLN, normal lymph node; BC, breast cancer; NL16, normal liver; F, forward; R, reverse. NL16 is a different patient with a (CA)4 repeat.

    Journal: Oncology Letters

    Article Title: Caution for simple sequence repeat number variation in the mitochondrial DNA D-loop to determine cancer-specific variants

    doi: 10.3892/ol.2018.9809

    Figure Lengend Snippet: Sequencing electropherogram of (CA)7 . PCR of the mtDNA D-loop (CA)7 was performed using EX Taq DNA polymerase and F7/R5 primer pairs (A) or F5/R5 primer pairs (B). The sequencing electropherogram using the F5 primer is the same as (data not shown). (C) PCR was performed using a different proofreading DNA polymerase KOD and F7/R5 primer pairs. (D) PCR of genomic (CA)7 of the NAALAD2 gene was performed using EX Taq DNA polymerase. An arrow indicates a representative position used to evaluate a variant allele. PCR, polymerase chain reaction; mtDNA, mitochondrial DNA; NB, normal breast epithelia; NLN, normal lymph node; BC, breast cancer; NL16, normal liver; F, forward; R, reverse. NL16 is a different patient with a (CA)4 repeat.

    Article Snippet: PCR was performed in 20-µl reactions using Takara EX Taq DNA polymerase Hot Start Version (Takara Bio Inc., Shiga, Japan) and KOD-Plus-Neo (Toyobo, Osaka, Japan) according to the manufacturer's instructions.

    Techniques: Sequencing, Polymerase Chain Reaction, Variant Assay

    HotStart and low-DNA Taq DNA polymerases are not sufficiently pure for sensitive and specific broad-range amplification of bacterial DNA. The genomic DNA (100 fg) of S. aureus was amplified by HotStart or low-DNA Taq DNA polymerases (Taq #1: Hot Start Taq DNA polymerase, Protech Inc.; Taq #2: Fast Hot Start Taq DNA polymerase, KAPA Biosystems; Taq #3: Taq DNA polymerase, TakaRa Inc.; Taq #4: ULTRATOOLS Taq DNA polymerase, Biotools Inc.) using the primer set p201 and p1370 (lanes 1, 3, 5, and 7). Significant amount of PCR product was present in the no template control reactions (lanes 2, 4, 6, and 8).

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Broad-Range Detection of Low Abundance Bacteria without DNA Decontamination of PCR Reagents

    doi: 10.1371/journal.pone.0020303

    Figure Lengend Snippet: HotStart and low-DNA Taq DNA polymerases are not sufficiently pure for sensitive and specific broad-range amplification of bacterial DNA. The genomic DNA (100 fg) of S. aureus was amplified by HotStart or low-DNA Taq DNA polymerases (Taq #1: Hot Start Taq DNA polymerase, Protech Inc.; Taq #2: Fast Hot Start Taq DNA polymerase, KAPA Biosystems; Taq #3: Taq DNA polymerase, TakaRa Inc.; Taq #4: ULTRATOOLS Taq DNA polymerase, Biotools Inc.) using the primer set p201 and p1370 (lanes 1, 3, 5, and 7). Significant amount of PCR product was present in the no template control reactions (lanes 2, 4, 6, and 8).

    Article Snippet: The Fast Hot Start Taq DNA polymerase was purchased from KAPA Biosystems (Woburn, MA).

    Techniques: Amplification, Polymerase Chain Reaction