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  • 92
    Solis BioDyne hot firepol multiplex mix
    Hot Firepol Multiplex Mix, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot firepol multiplex mix/product/Solis BioDyne
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot firepol multiplex mix - by Bioz Stars, 2022-07
    92/100 stars
      Buy from Supplier

    92
    Solis BioDyne hot firepol multiplex mix ready
    Sensitivity of the newly described <t>multiplex</t> PCR method compared to the corresponding simplex PCR for each of the targeted bacterial taxon. Every reaction was performed with six samples of corresponding control bacteria at different concentrations. Lane 1: 1 ng/μl, lane 2: 0.5 ng/μl, lane 3: 0.1 ng/μl, lane 4: 0.05 ng/μl and lane 5: 0.01ng/μl, lane 6: water control. a-b: P . fuscovaginae strain UBP735, c-d: B . glumae strain NCPPB 3923, e-f: Sphingomonas strain V1-2, g-h: X . oryzae pv. oryzae strain BAI10, i-j: Pantoea strain ARC10. a, c, e, g, i: simplex PCR with only one primer pair in each case. b, d, f, h, j: multiplex PCR including the five primer pairs.
    Hot Firepol Multiplex Mix Ready, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot firepol multiplex mix ready/product/Solis BioDyne
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot firepol multiplex mix ready - by Bioz Stars, 2022-07
    92/100 stars
      Buy from Supplier

    94
    Solis BioDyne single qpcr plate
    Correct ECM structure in the median fin fold and regenerating caudal fins is hampered in fndc3a wue1/wue1 mutants. (A) F-actin in the median fin fold 22hpf was visualized by phalloidin staining, (B) localization of β-catenin during fin development was visualized by immunofluorescence of 24hpf embryos (n=6 for each group). Both colorations mark cell membranes and ECM structures. Cellular organization of ventral median fin fold cells and ECM matrix is symmetrically structured in control embryos and shows nuclear localization of active Wnt signals in cells at the fin fold tip (white arrowheads in B). fndc3a wue1/wue1 mutants depict cellular alterations and unstructured ECM assembly by showing irregular cell shapes (white arrows in A), cavities within the fin fold (white arrowheads in A2) and speckled accumulation of β-catenin between cells (white arrows in B). Nuclear localization of β-catenin in cells at the fin fold tip was maintained (grey arrowheads in B). (C and D; n=4 for each group) Fin regenerates of fndc3a wue1/wue1 mutants incubated at 32°C and stained for F-actin showed regenerate abnormalities (white arrows in C), irregular regenerate borders (white dashed lines in C) and cellular cavities (white arrowheads in D) (E and F; n=3 for each group) Fin regenerates of fndc3a wue1/wue1 mutants also depicted intracellular accumulation of β-catenin, divergent ECM assembly (white arrows) in addition to appearance of abnormal cells loosely attached to the regenerate (white arrowheads). Images either show maximum intensity projections (30 to 40 <t>single</t> z-slices; z-distance: 1.5µm) or a representative higher resolution single z slice.
    Single Qpcr Plate, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single qpcr plate/product/Solis BioDyne
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    single qpcr plate - by Bioz Stars, 2022-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Sensitivity of the newly described multiplex PCR method compared to the corresponding simplex PCR for each of the targeted bacterial taxon. Every reaction was performed with six samples of corresponding control bacteria at different concentrations. Lane 1: 1 ng/μl, lane 2: 0.5 ng/μl, lane 3: 0.1 ng/μl, lane 4: 0.05 ng/μl and lane 5: 0.01ng/μl, lane 6: water control. a-b: P . fuscovaginae strain UBP735, c-d: B . glumae strain NCPPB 3923, e-f: Sphingomonas strain V1-2, g-h: X . oryzae pv. oryzae strain BAI10, i-j: Pantoea strain ARC10. a, c, e, g, i: simplex PCR with only one primer pair in each case. b, d, f, h, j: multiplex PCR including the five primer pairs.

    Journal: PLoS ONE

    Article Title: Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

    doi: 10.1371/journal.pone.0232115

    Figure Lengend Snippet: Sensitivity of the newly described multiplex PCR method compared to the corresponding simplex PCR for each of the targeted bacterial taxon. Every reaction was performed with six samples of corresponding control bacteria at different concentrations. Lane 1: 1 ng/μl, lane 2: 0.5 ng/μl, lane 3: 0.1 ng/μl, lane 4: 0.05 ng/μl and lane 5: 0.01ng/μl, lane 6: water control. a-b: P . fuscovaginae strain UBP735, c-d: B . glumae strain NCPPB 3923, e-f: Sphingomonas strain V1-2, g-h: X . oryzae pv. oryzae strain BAI10, i-j: Pantoea strain ARC10. a, c, e, g, i: simplex PCR with only one primer pair in each case. b, d, f, h, j: multiplex PCR including the five primer pairs.

    Article Snippet: Following the optimization, the final conditions for the multiplex PCR protocol were: 2 μl of DNA added to 23 μl master mix comprising 5 μl Hot Firepol Multiplex Mix ready to load 5X (Solis BioDyne,Tartu, Estonia), 1.25 μl (NH4 )2 SO4 (160 mM), 0.2 μl of each primers specific of P . fuscovaginae at 5 μM, 0.2 μl of each primers specific of B . gluma e and B . gladioli at 100 μM, 0.3 μl of each primers specific of Pantoea spp. at 100 μM and 0.3 μl of each primers specific of X . oryzae at 10 μM and finally 0.1 μl of each primers specific of Sphingomonas spp. at 10 μM.

    Techniques: Multiplex Assay, Polymerase Chain Reaction

    Detection of the five bacterial taxa using the newly described multiplex PCR protocol. L: molecular size marker, 100 bp DNA ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.

    Journal: PLoS ONE

    Article Title: Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

    doi: 10.1371/journal.pone.0232115

    Figure Lengend Snippet: Detection of the five bacterial taxa using the newly described multiplex PCR protocol. L: molecular size marker, 100 bp DNA ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.

    Article Snippet: Following the optimization, the final conditions for the multiplex PCR protocol were: 2 μl of DNA added to 23 μl master mix comprising 5 μl Hot Firepol Multiplex Mix ready to load 5X (Solis BioDyne,Tartu, Estonia), 1.25 μl (NH4 )2 SO4 (160 mM), 0.2 μl of each primers specific of P . fuscovaginae at 5 μM, 0.2 μl of each primers specific of B . gluma e and B . gladioli at 100 μM, 0.3 μl of each primers specific of Pantoea spp. at 100 μM and 0.3 μl of each primers specific of X . oryzae at 10 μM and finally 0.1 μl of each primers specific of Sphingomonas spp. at 10 μM.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Marker

    Correct ECM structure in the median fin fold and regenerating caudal fins is hampered in fndc3a wue1/wue1 mutants. (A) F-actin in the median fin fold 22hpf was visualized by phalloidin staining, (B) localization of β-catenin during fin development was visualized by immunofluorescence of 24hpf embryos (n=6 for each group). Both colorations mark cell membranes and ECM structures. Cellular organization of ventral median fin fold cells and ECM matrix is symmetrically structured in control embryos and shows nuclear localization of active Wnt signals in cells at the fin fold tip (white arrowheads in B). fndc3a wue1/wue1 mutants depict cellular alterations and unstructured ECM assembly by showing irregular cell shapes (white arrows in A), cavities within the fin fold (white arrowheads in A2) and speckled accumulation of β-catenin between cells (white arrows in B). Nuclear localization of β-catenin in cells at the fin fold tip was maintained (grey arrowheads in B). (C and D; n=4 for each group) Fin regenerates of fndc3a wue1/wue1 mutants incubated at 32°C and stained for F-actin showed regenerate abnormalities (white arrows in C), irregular regenerate borders (white dashed lines in C) and cellular cavities (white arrowheads in D) (E and F; n=3 for each group) Fin regenerates of fndc3a wue1/wue1 mutants also depicted intracellular accumulation of β-catenin, divergent ECM assembly (white arrows) in addition to appearance of abnormal cells loosely attached to the regenerate (white arrowheads). Images either show maximum intensity projections (30 to 40 single z-slices; z-distance: 1.5µm) or a representative higher resolution single z slice.

    Journal: bioRxiv

    Article Title: Fndc3a (Fibronectin Domain Containing Protein 3A) influences median fin fold development and caudal fin regeneration in zebrafish by ECM alteration

    doi: 10.1101/386813

    Figure Lengend Snippet: Correct ECM structure in the median fin fold and regenerating caudal fins is hampered in fndc3a wue1/wue1 mutants. (A) F-actin in the median fin fold 22hpf was visualized by phalloidin staining, (B) localization of β-catenin during fin development was visualized by immunofluorescence of 24hpf embryos (n=6 for each group). Both colorations mark cell membranes and ECM structures. Cellular organization of ventral median fin fold cells and ECM matrix is symmetrically structured in control embryos and shows nuclear localization of active Wnt signals in cells at the fin fold tip (white arrowheads in B). fndc3a wue1/wue1 mutants depict cellular alterations and unstructured ECM assembly by showing irregular cell shapes (white arrows in A), cavities within the fin fold (white arrowheads in A2) and speckled accumulation of β-catenin between cells (white arrows in B). Nuclear localization of β-catenin in cells at the fin fold tip was maintained (grey arrowheads in B). (C and D; n=4 for each group) Fin regenerates of fndc3a wue1/wue1 mutants incubated at 32°C and stained for F-actin showed regenerate abnormalities (white arrows in C), irregular regenerate borders (white dashed lines in C) and cellular cavities (white arrowheads in D) (E and F; n=3 for each group) Fin regenerates of fndc3a wue1/wue1 mutants also depicted intracellular accumulation of β-catenin, divergent ECM assembly (white arrows) in addition to appearance of abnormal cells loosely attached to the regenerate (white arrowheads). Images either show maximum intensity projections (30 to 40 single z-slices; z-distance: 1.5µm) or a representative higher resolution single z slice.

    Article Snippet: Each group and primer sample was analyzed in triplicates on a single qPCR plate utilizing HOT FIREPol Eva Green Mix Plus (Solis Biodyne).

    Techniques: Staining, Immunofluorescence, Incubation

    Generation and phenotype of fndc3a wue1,wue1 zebrafish mutants. (A) The CRISPR/Cas9 system was used to target exon 13 in the zebrafish fndc3a gene coding for the third fibronectin type III domain (nucleotides marked in blue indicate sgRNA target sequence; nucleotides marked in red indicate the region of mutated sequence). (B-D) fndc3a wue1/wue1 mutants show straightened tail buds (n=19/40), kinked tails (n= 27/100), and actinotrichia fiber aggregation during the first days of embryonic development (n= 9/41; indicated by arrows). (E) A fraction of adult fndc3a wue1/wue1 mutants display weak (n= 15/71) to strong (n= 6/71) caudal fin phenotypes and tail malformations. (F) qPCR quantification of relative fndc3a expression levels in genotypic different groups of embryos indicated reduction of fndc3a transcripts in fndc3a wue1/+ and fndc3a Nue1/Nue1 (ΔΔCt calculation; comparison of biological triplicates for each genotype with twelve 24hpf embryos each; two independent fndc3a primer pairs; normalized against AB controls; gapdh and ef1a1l1 expression were used as endogenous/housekeeping controls; significance levels and p-values of a 2-sided paired student t-test are given). Scale bars for embryo overview: 250pm; scale bars for tail magnifications: 100µm.

    Journal: bioRxiv

    Article Title: Fndc3a (Fibronectin Domain Containing Protein 3A) influences median fin fold development and caudal fin regeneration in zebrafish by ECM alteration

    doi: 10.1101/386813

    Figure Lengend Snippet: Generation and phenotype of fndc3a wue1,wue1 zebrafish mutants. (A) The CRISPR/Cas9 system was used to target exon 13 in the zebrafish fndc3a gene coding for the third fibronectin type III domain (nucleotides marked in blue indicate sgRNA target sequence; nucleotides marked in red indicate the region of mutated sequence). (B-D) fndc3a wue1/wue1 mutants show straightened tail buds (n=19/40), kinked tails (n= 27/100), and actinotrichia fiber aggregation during the first days of embryonic development (n= 9/41; indicated by arrows). (E) A fraction of adult fndc3a wue1/wue1 mutants display weak (n= 15/71) to strong (n= 6/71) caudal fin phenotypes and tail malformations. (F) qPCR quantification of relative fndc3a expression levels in genotypic different groups of embryos indicated reduction of fndc3a transcripts in fndc3a wue1/+ and fndc3a Nue1/Nue1 (ΔΔCt calculation; comparison of biological triplicates for each genotype with twelve 24hpf embryos each; two independent fndc3a primer pairs; normalized against AB controls; gapdh and ef1a1l1 expression were used as endogenous/housekeeping controls; significance levels and p-values of a 2-sided paired student t-test are given). Scale bars for embryo overview: 250pm; scale bars for tail magnifications: 100µm.

    Article Snippet: Each group and primer sample was analyzed in triplicates on a single qPCR plate utilizing HOT FIREPol Eva Green Mix Plus (Solis Biodyne).

    Techniques: CRISPR, Sequencing, Real-time Polymerase Chain Reaction, Expressing