Journal: PLOS Neglected Tropical Diseases

Article Title: A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”

doi: 10.1371/journal.pntd.0010908

Figure Lengend Snippet: Serial 10-fold dilution from 6.4x10 6 U/mL to 6.4x10 0 U/mL of M . ulcerans DNA. The standard curve was generated by a linear regression of the threshold cycle (Ct) against the logarithm of M . ulcerans DNA concentration. Each 10-fold dilution was performed in duplicate for n = 3 experiments, one standard deviation (SD) is shown. (A) Primers/probe set 1: Brillant II ( y = −3.16 x + 41.37); Hot FirePol ( y = −3.307 x + 42.44). (B) Primers/probe set 2: Brillant II ( y = −3.217 x + 42.31); Hot FirePol ( y = −3.147 x + 41.84).

Article Snippet: The two master mix are comparable; however, we decided to select the HOT FIREPol Probe Universal qPCR Mix for BU-LABNET laboratories because it is guaranteed stable at room temperature for up to a month, ideally suitable for a shipment at room temperature from Europe to African countries.

Techniques: Generated, Concentration Assay, Standard Deviation