horseradish peroxidase-conjugated anti-mouse Search Results


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  • 99
    Millipore horseradish peroxidase conjugated anti mouse
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer horseradish peroxidase conjugated anti mouse
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare horseradish peroxidase conjugated anti mouse
    Chronic ethanol ingestion is fibrogenic in rat kidney. A) Fibrotic protein expression in kidney. Rats were fed the standard Lieber-deCarli liquid ethanol diet, or its isomaltose control, for 28 days before kidneys were excised, perfused with saline, fixed and sectioned as described in “Methods.” Sections were rehydrated and stained with <t>anti-TGF-ß1,</t> anti- α-smooth muscle actin, anti-collagen IV or anti-collagen 1 antibodies, and <t>SP-streptavidin-conjugated</t> secondary antibody for anti-TGF-ß1, or fluorescent secondary antibodies for the other sections as stated in “Methods.” TGF-ß sections were developed with <t>horseradish</t> <t>peroxidase</t> and DAB/metal chromogenic solution. B) mRNAs encoding fibrotic proteins were induced in kidneys of rats ingesting ethanol. Total RNA was isolated from ethanol and pair-fed kidneys from rats at the end of the feeding trial, and mRNA was quantified by SYBR Green one-step Reverse Transcription-PCR for the stated mRNAs and ribosomal 18S with the Bio-Rad MyiQ real-time PCR detection system. mRNA expression was normalized to 18S RNA content and 2 -ΔΔCT was used to calculate the fold changes. Data are expressed as mean±SEM (n = 4), and p
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad horseradish peroxidase conjugated anti mouse
    Chronic ethanol ingestion is fibrogenic in rat kidney. A) Fibrotic protein expression in kidney. Rats were fed the standard Lieber-deCarli liquid ethanol diet, or its isomaltose control, for 28 days before kidneys were excised, perfused with saline, fixed and sectioned as described in “Methods.” Sections were rehydrated and stained with <t>anti-TGF-ß1,</t> anti- α-smooth muscle actin, anti-collagen IV or anti-collagen 1 antibodies, and <t>SP-streptavidin-conjugated</t> secondary antibody for anti-TGF-ß1, or fluorescent secondary antibodies for the other sections as stated in “Methods.” TGF-ß sections were developed with <t>horseradish</t> <t>peroxidase</t> and DAB/metal chromogenic solution. B) mRNAs encoding fibrotic proteins were induced in kidneys of rats ingesting ethanol. Total RNA was isolated from ethanol and pair-fed kidneys from rats at the end of the feeding trial, and mRNA was quantified by SYBR Green one-step Reverse Transcription-PCR for the stated mRNAs and ribosomal 18S with the Bio-Rad MyiQ real-time PCR detection system. mRNA expression was normalized to 18S RNA content and 2 -ΔΔCT was used to calculate the fold changes. Data are expressed as mean±SEM (n = 4), and p
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies horseradish peroxidase conjugated anti mouse
    Chronic ethanol ingestion is fibrogenic in rat kidney. A) Fibrotic protein expression in kidney. Rats were fed the standard Lieber-deCarli liquid ethanol diet, or its isomaltose control, for 28 days before kidneys were excised, perfused with saline, fixed and sectioned as described in “Methods.” Sections were rehydrated and stained with <t>anti-TGF-ß1,</t> anti- α-smooth muscle actin, anti-collagen IV or anti-collagen 1 antibodies, and <t>SP-streptavidin-conjugated</t> secondary antibody for anti-TGF-ß1, or fluorescent secondary antibodies for the other sections as stated in “Methods.” TGF-ß sections were developed with <t>horseradish</t> <t>peroxidase</t> and DAB/metal chromogenic solution. B) mRNAs encoding fibrotic proteins were induced in kidneys of rats ingesting ethanol. Total RNA was isolated from ethanol and pair-fed kidneys from rats at the end of the feeding trial, and mRNA was quantified by SYBR Green one-step Reverse Transcription-PCR for the stated mRNAs and ribosomal 18S with the Bio-Rad MyiQ real-time PCR detection system. mRNA expression was normalized to 18S RNA content and 2 -ΔΔCT was used to calculate the fold changes. Data are expressed as mean±SEM (n = 4), and p
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology horseradish peroxidase conjugated anti mouse
    Chronic ethanol ingestion is fibrogenic in rat kidney. A) Fibrotic protein expression in kidney. Rats were fed the standard Lieber-deCarli liquid ethanol diet, or its isomaltose control, for 28 days before kidneys were excised, perfused with saline, fixed and sectioned as described in “Methods.” Sections were rehydrated and stained with <t>anti-TGF-ß1,</t> anti- α-smooth muscle actin, anti-collagen IV or anti-collagen 1 antibodies, and <t>SP-streptavidin-conjugated</t> secondary antibody for anti-TGF-ß1, or fluorescent secondary antibodies for the other sections as stated in “Methods.” TGF-ß sections were developed with <t>horseradish</t> <t>peroxidase</t> and DAB/metal chromogenic solution. B) mRNAs encoding fibrotic proteins were induced in kidneys of rats ingesting ethanol. Total RNA was isolated from ethanol and pair-fed kidneys from rats at the end of the feeding trial, and mRNA was quantified by SYBR Green one-step Reverse Transcription-PCR for the stated mRNAs and ribosomal 18S with the Bio-Rad MyiQ real-time PCR detection system. mRNA expression was normalized to 18S RNA content and 2 -ΔΔCT was used to calculate the fold changes. Data are expressed as mean±SEM (n = 4), and p
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti mouse horseradish peroxidase conjugate
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Anti Mouse Horseradish Peroxidase Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad anti mouse horseradish peroxidase conjugates
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Anti Mouse Horseradish Peroxidase Conjugates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti mouse horseradish peroxidase conjugates
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Anti Mouse Horseradish Peroxidase Conjugates, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare anti mouse horseradish peroxidase conjugate
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Anti Mouse Horseradish Peroxidase Conjugate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad anti mouse horseradish peroxidase conjugate
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Anti Mouse Horseradish Peroxidase Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti mouse horseradish peroxidase conjugate
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Anti Mouse Horseradish Peroxidase Conjugate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies anti mouse horseradish peroxidase conjugate
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Anti Mouse Horseradish Peroxidase Conjugate, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory horseradish peroxidase conjugated anti mouse
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson horseradish peroxidase conjugated anti mouse
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore horseradish peroxidase hrp conjugated anti mouse
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Horseradish Peroxidase Hrp Conjugated Anti Mouse, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam horseradish peroxidase conjugated anti mouse
    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a <t>horseradish</t> <t>peroxidase-conjugated</t> <t>anti-mouse</t> or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.
    Horseradish Peroxidase Conjugated Anti Mouse, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare anti mouse horseradish peroxidase conjugates
    Identification of 14-3-3 isoforms recognized by three mAbs generated from 14-3-3/G-box DNA-binding complexes using 13 recombinant Arabidopsis 14-3-3 proteins. A, GBC mAbs detect a subset of nondenatured recombinant Arabidopsis 14-3-3 proteins. Samples of each purified recombinant Arabidopsis 14-3-3 protein were transferred to nitrocellulose with a dot-blot apparatus. After blocking, the blot was probed with GBC mAb 2A3, 4B9, and 5D6. Cross-reactivity was detected with <t>anti-mouse</t> <t>horseradish</t> <t>peroxidase</t> conjugate and chemiluminescence. B, GBC mAbs detect a subset of denatured recombinant Arabidopsis 14-3-3 proteins. Aliquots of 10 μ g of each recombinant Arabidopsis 14-3-3 proteins were denatured in reducing SDS sample buffer prior to electrophoresis. After transferring electrophoresed proteins to nitrocellulose and blocking, the blot was probed with GBC mAb 2A3, 4B9, and 5D6. Immuno-cross-reactivity was detected with anti-mouse horseradish peroxidase conjugate and chemiluminescence.
    Anti Mouse Horseradish Peroxidase Conjugates, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Chronic ethanol ingestion is fibrogenic in rat kidney. A) Fibrotic protein expression in kidney. Rats were fed the standard Lieber-deCarli liquid ethanol diet, or its isomaltose control, for 28 days before kidneys were excised, perfused with saline, fixed and sectioned as described in “Methods.” Sections were rehydrated and stained with anti-TGF-ß1, anti- α-smooth muscle actin, anti-collagen IV or anti-collagen 1 antibodies, and SP-streptavidin-conjugated secondary antibody for anti-TGF-ß1, or fluorescent secondary antibodies for the other sections as stated in “Methods.” TGF-ß sections were developed with horseradish peroxidase and DAB/metal chromogenic solution. B) mRNAs encoding fibrotic proteins were induced in kidneys of rats ingesting ethanol. Total RNA was isolated from ethanol and pair-fed kidneys from rats at the end of the feeding trial, and mRNA was quantified by SYBR Green one-step Reverse Transcription-PCR for the stated mRNAs and ribosomal 18S with the Bio-Rad MyiQ real-time PCR detection system. mRNA expression was normalized to 18S RNA content and 2 -ΔΔCT was used to calculate the fold changes. Data are expressed as mean±SEM (n = 4), and p

    Journal: PLoS ONE

    Article Title: Inflammatory PAF Receptor Signaling Initiates Hedgehog Signaling and Kidney Fibrogenesis During Ethanol Consumption

    doi: 10.1371/journal.pone.0145691

    Figure Lengend Snippet: Chronic ethanol ingestion is fibrogenic in rat kidney. A) Fibrotic protein expression in kidney. Rats were fed the standard Lieber-deCarli liquid ethanol diet, or its isomaltose control, for 28 days before kidneys were excised, perfused with saline, fixed and sectioned as described in “Methods.” Sections were rehydrated and stained with anti-TGF-ß1, anti- α-smooth muscle actin, anti-collagen IV or anti-collagen 1 antibodies, and SP-streptavidin-conjugated secondary antibody for anti-TGF-ß1, or fluorescent secondary antibodies for the other sections as stated in “Methods.” TGF-ß sections were developed with horseradish peroxidase and DAB/metal chromogenic solution. B) mRNAs encoding fibrotic proteins were induced in kidneys of rats ingesting ethanol. Total RNA was isolated from ethanol and pair-fed kidneys from rats at the end of the feeding trial, and mRNA was quantified by SYBR Green one-step Reverse Transcription-PCR for the stated mRNAs and ribosomal 18S with the Bio-Rad MyiQ real-time PCR detection system. mRNA expression was normalized to 18S RNA content and 2 -ΔΔCT was used to calculate the fold changes. Data are expressed as mean±SEM (n = 4), and p

    Article Snippet: The conjugates were then ligated by horseradish peroxidase-conjugated anti-mouse (1:10000) or anti-rabbit (1:5000) antibody before detection with Amersham Biosciences ECL Prime.

    Techniques: Expressing, Staining, Isolation, SYBR Green Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a horseradish peroxidase-conjugated anti-mouse or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.

    Journal: Journal of Virology

    Article Title: Immobilized Cobalt Affinity Chromatography Provides a Novel, Efficient Method for Herpes Simplex Virus Type 1 Gene Vector Purification

    doi: 10.1128/JVI.78.17.8994-9006.2004

    Figure Lengend Snippet: Western blot analysis of HAT-tagged gB. Protein lysates from Vero cells infected with KgBHAT (lanes 2 and 4) and the control virus KgBpK − (lanes 1 and 3) were separated by using the NuPAGE system and were transferred to a polyvinylidene difluoride membrane. The membrane was then cut in half, and each part was probed separately either with a monoclonal antibody against HSV gB (lanes 1 and 2) or with a polyclonal antibody against the HAT peptide (lanes 3 and 4), followed by detection with a horseradish peroxidase-conjugated anti-mouse or anti-rabbit antibody, respectively. M, molecular size marker; Ab, antibody.

    Article Snippet: The membranes were then washed with PBS containing 0.05% Tween 20 (Sigma, St. Louis, Mo.) and incubated with either anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibodies (Sigma) in 2% milk-PBS for 1 h at 25°C.

    Techniques: Western Blot, HAT Assay, Infection, Marker

    Identification of 14-3-3 isoforms recognized by three mAbs generated from 14-3-3/G-box DNA-binding complexes using 13 recombinant Arabidopsis 14-3-3 proteins. A, GBC mAbs detect a subset of nondenatured recombinant Arabidopsis 14-3-3 proteins. Samples of each purified recombinant Arabidopsis 14-3-3 protein were transferred to nitrocellulose with a dot-blot apparatus. After blocking, the blot was probed with GBC mAb 2A3, 4B9, and 5D6. Cross-reactivity was detected with anti-mouse horseradish peroxidase conjugate and chemiluminescence. B, GBC mAbs detect a subset of denatured recombinant Arabidopsis 14-3-3 proteins. Aliquots of 10 μ g of each recombinant Arabidopsis 14-3-3 proteins were denatured in reducing SDS sample buffer prior to electrophoresis. After transferring electrophoresed proteins to nitrocellulose and blocking, the blot was probed with GBC mAb 2A3, 4B9, and 5D6. Immuno-cross-reactivity was detected with anti-mouse horseradish peroxidase conjugate and chemiluminescence.

    Journal: Plant Physiology

    Article Title: Exposed Loop Domains of Complexed 14-3-3 Proteins Contribute to Structural Diversity and Functional Specificity 1

    doi: 10.1104/pp.105.073916

    Figure Lengend Snippet: Identification of 14-3-3 isoforms recognized by three mAbs generated from 14-3-3/G-box DNA-binding complexes using 13 recombinant Arabidopsis 14-3-3 proteins. A, GBC mAbs detect a subset of nondenatured recombinant Arabidopsis 14-3-3 proteins. Samples of each purified recombinant Arabidopsis 14-3-3 protein were transferred to nitrocellulose with a dot-blot apparatus. After blocking, the blot was probed with GBC mAb 2A3, 4B9, and 5D6. Cross-reactivity was detected with anti-mouse horseradish peroxidase conjugate and chemiluminescence. B, GBC mAbs detect a subset of denatured recombinant Arabidopsis 14-3-3 proteins. Aliquots of 10 μ g of each recombinant Arabidopsis 14-3-3 proteins were denatured in reducing SDS sample buffer prior to electrophoresis. After transferring electrophoresed proteins to nitrocellulose and blocking, the blot was probed with GBC mAb 2A3, 4B9, and 5D6. Immuno-cross-reactivity was detected with anti-mouse horseradish peroxidase conjugate and chemiluminescence.

    Article Snippet: Anti-rabbit and anti-mouse horseradish peroxidase conjugates were purchased from Amersham (Amersham Biosciences).

    Techniques: Generated, Binding Assay, Recombinant, Purification, Dot Blot, Blocking Assay, Electrophoresis, Transferring