hogg1 New England Biolabs Search Results


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  • 95
    New England Biolabs hogg1
    (A) Variance of the initial velocity in response to various concentrations of the Cy3-labeled molecular beacon substrate. The concentration of <t>hOGG1</t> is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . (B) Variance of the initial velocity in response to various concentrations of the Cy5-labeled molecular beacon substrate. The concentration of hAAG is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . The error bars represent the standard deviations of the three experiments.
    Hogg1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hogg1/product/New England Biolabs
    Average 95 stars, based on 132 article reviews
    Price from $9.99 to $1999.99
    hogg1 - by Bioz Stars, 2020-07
    95/100 stars
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    99
    New England Biolabs repair endonuclease hogg1
    (A) Variance of the initial velocity in response to various concentrations of the Cy3-labeled molecular beacon substrate. The concentration of <t>hOGG1</t> is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . (B) Variance of the initial velocity in response to various concentrations of the Cy5-labeled molecular beacon substrate. The concentration of hAAG is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . The error bars represent the standard deviations of the three experiments.
    Repair Endonuclease Hogg1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/repair endonuclease hogg1/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    repair endonuclease hogg1 - by Bioz Stars, 2020-07
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    94
    New England Biolabs human ogg1 hogg1 treatment
    (A) Variance of the initial velocity in response to various concentrations of the Cy3-labeled molecular beacon substrate. The concentration of <t>hOGG1</t> is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . (B) Variance of the initial velocity in response to various concentrations of the Cy5-labeled molecular beacon substrate. The concentration of hAAG is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . The error bars represent the standard deviations of the three experiments.
    Human Ogg1 Hogg1 Treatment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human ogg1 hogg1 treatment/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    95
    New England Biolabs human 8 oxoguanine dna glycosylase
    The level of <t>DNA</t> pyrimidine and purine oxidation in HUVECs (analysis by alkaline comet assay using endonuclease III (Nth)) or <t>human</t> 8 <t>oxoguanine</t> DNA <t>glycosylase</t> (hOOG1) was induced by 25-hydroxycholesterol (10 µg/mL) ( A ). The repair of DNA damage in cells was induced by ( B ) dabigatran (100 ng/mL and 500 ng/mL) and ( C ) rivaroxaban (100 ng/mL and 500 ng/mL). Each experiment included a positive control (PC) which concerned the cells incubated with hydrogen peroxide at 20 µM for 15 min on ice and subsequently treated with the enzymes. The value of DNA in the figure captions for comet tail in the presence of either enzyme for all groups was reduced by the value obtained in the comet assay without any enzyme and the value for enzyme buffer only. The number of cells scored from each slide was 100. The mean value for 100 cells analyzed in each treatment in four independent experiments (400 total cells) was recorded. Mean ± SEM was calculated from four individual experiments (400 comets) and was significantly different from negative controls at * p
    Human 8 Oxoguanine Dna Glycosylase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human 8 oxoguanine dna glycosylase/product/New England Biolabs
    Average 95 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    human 8 oxoguanine dna glycosylase - by Bioz Stars, 2020-07
    95/100 stars
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    Image Search Results


    (A) Variance of the initial velocity in response to various concentrations of the Cy3-labeled molecular beacon substrate. The concentration of hOGG1 is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . (B) Variance of the initial velocity in response to various concentrations of the Cy5-labeled molecular beacon substrate. The concentration of hAAG is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . The error bars represent the standard deviations of the three experiments.

    Journal: Chemical Science

    Article Title: Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level †Electronic supplementary information (ESI) available: Molecular mechanism of the DNA glycosylase-mediated cleavage of molecular beacons and optimization of the experimental conditions. See DOI: 10.1039/c7sc04296e

    doi: 10.1039/c7sc04296e

    Figure Lengend Snippet: (A) Variance of the initial velocity in response to various concentrations of the Cy3-labeled molecular beacon substrate. The concentration of hOGG1 is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . (B) Variance of the initial velocity in response to various concentrations of the Cy5-labeled molecular beacon substrate. The concentration of hAAG is 0.1 U μL –1 and the concentration of APE1 is 0.1 U μL –1 . The error bars represent the standard deviations of the three experiments.

    Article Snippet: Then, 1 μL of the products of the molecular beacon (the final concentration of each substrate is 0.3 μM) were added into 20 μL of the reaction solution containing a varied concentration of hOGG1 and/or hAAG, 1× NEBuffer 2, 100 μg mL–1 BSA, 1× NEBuffer 4, 1× ThermoPol buffer and 0.1 U μL–1 APE1, followed by incubation at 37 °C for 1.5 h. The reaction was terminated by heating at 80 °C for 20 min.

    Techniques: Labeling, Concentration Assay

    Variance of the relative catalytic activity with different concentrations of Cd 2+ for hOGG1 alone (black line), hOGG1 + APE1 (red line) and hAAG + APE1 (blue line). The Cy3-labeled molecular beacon (0.3 μM), the Cy5-labeled molecular beacon (0.3 μM), hOGG1 (0.1 U μL –1 ), hAAG (0.1 U μL –1 ) and APE1 (0.1 U μL –1 ) were used in this research. The error bars represent the standard deviations of the three experiments.

    Journal: Chemical Science

    Article Title: Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level †Electronic supplementary information (ESI) available: Molecular mechanism of the DNA glycosylase-mediated cleavage of molecular beacons and optimization of the experimental conditions. See DOI: 10.1039/c7sc04296e

    doi: 10.1039/c7sc04296e

    Figure Lengend Snippet: Variance of the relative catalytic activity with different concentrations of Cd 2+ for hOGG1 alone (black line), hOGG1 + APE1 (red line) and hAAG + APE1 (blue line). The Cy3-labeled molecular beacon (0.3 μM), the Cy5-labeled molecular beacon (0.3 μM), hOGG1 (0.1 U μL –1 ), hAAG (0.1 U μL –1 ) and APE1 (0.1 U μL –1 ) were used in this research. The error bars represent the standard deviations of the three experiments.

    Article Snippet: Then, 1 μL of the products of the molecular beacon (the final concentration of each substrate is 0.3 μM) were added into 20 μL of the reaction solution containing a varied concentration of hOGG1 and/or hAAG, 1× NEBuffer 2, 100 μg mL–1 BSA, 1× NEBuffer 4, 1× ThermoPol buffer and 0.1 U μL–1 APE1, followed by incubation at 37 °C for 1.5 h. The reaction was terminated by heating at 80 °C for 20 min.

    Techniques: Activity Assay, Labeling

    (A) PAGE analysis of the hOGG1-mediated cleavage of the Cy3-labeled molecular beacon (lanes 1–4) and the hAAG-mediated cleavage of the Cy5-labeled molecular beacon (lanes 5–8) with SYBR Gold as the indicator. (B) PAGE analysis of the hOGG1-mediated cleavage of the Cy3-labeled molecular beacon and the hAAG-mediated cleavage of the Cy5-labeled molecular beacon by excitation of Cy3 and Cy5. The green color indicates the Cy3-labeled DNA fragment in the presence of hOGG1 (lane 2) and the red color indicates the Cy5-labeled DNA fragment in the presence of hAAG (lane 4). (C) Fluorescence measurements of the hOGG1-mediated cleavage of the Cy3-labeled molecular beacon in the absence (black line) and presence (green line) of hOGG1. (D) Fluorescence measurements of the hAAG-mediated cleavage of the Cy5-labeled molecular beacon in the absence (blue line) and presence (red line) of hAAG. The hOGG1 concentration is 0.1 U μL –1 and the hAAG concentration is 0.1 U μL –1 , and the APE1 concentration is 0.1 U μL –1 .

    Journal: Chemical Science

    Article Title: Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level †Electronic supplementary information (ESI) available: Molecular mechanism of the DNA glycosylase-mediated cleavage of molecular beacons and optimization of the experimental conditions. See DOI: 10.1039/c7sc04296e

    doi: 10.1039/c7sc04296e

    Figure Lengend Snippet: (A) PAGE analysis of the hOGG1-mediated cleavage of the Cy3-labeled molecular beacon (lanes 1–4) and the hAAG-mediated cleavage of the Cy5-labeled molecular beacon (lanes 5–8) with SYBR Gold as the indicator. (B) PAGE analysis of the hOGG1-mediated cleavage of the Cy3-labeled molecular beacon and the hAAG-mediated cleavage of the Cy5-labeled molecular beacon by excitation of Cy3 and Cy5. The green color indicates the Cy3-labeled DNA fragment in the presence of hOGG1 (lane 2) and the red color indicates the Cy5-labeled DNA fragment in the presence of hAAG (lane 4). (C) Fluorescence measurements of the hOGG1-mediated cleavage of the Cy3-labeled molecular beacon in the absence (black line) and presence (green line) of hOGG1. (D) Fluorescence measurements of the hAAG-mediated cleavage of the Cy5-labeled molecular beacon in the absence (blue line) and presence (red line) of hAAG. The hOGG1 concentration is 0.1 U μL –1 and the hAAG concentration is 0.1 U μL –1 , and the APE1 concentration is 0.1 U μL –1 .

    Article Snippet: Then, 1 μL of the products of the molecular beacon (the final concentration of each substrate is 0.3 μM) were added into 20 μL of the reaction solution containing a varied concentration of hOGG1 and/or hAAG, 1× NEBuffer 2, 100 μg mL–1 BSA, 1× NEBuffer 4, 1× ThermoPol buffer and 0.1 U μL–1 APE1, followed by incubation at 37 °C for 1.5 h. The reaction was terminated by heating at 80 °C for 20 min.

    Techniques: Polyacrylamide Gel Electrophoresis, Labeling, Fluorescence, Concentration Assay

    Simultaneous detection of multiple DNA glycosylases by TIRF-based single-molecule imaging in the absence (A and E) and presence of hOGG1 (B and F), hAAG (C and G) and both hOGG1 and hAAG (D and H). The Cy3 fluorescence signals are shown in green, and the Cy5 fluorescence signals are shown in red. The Cy3-labeled molecular beacon (0.3 μM), the Cy5-labeled molecular beacon (0.3 μM), hOGG1 (0.1 U μL –1 ), hAAG (0.1 U μL –1 ) and APE1 (0.1 U μL –1 ) were used in this research. The scale bar is 5 μm.

    Journal: Chemical Science

    Article Title: Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level †Electronic supplementary information (ESI) available: Molecular mechanism of the DNA glycosylase-mediated cleavage of molecular beacons and optimization of the experimental conditions. See DOI: 10.1039/c7sc04296e

    doi: 10.1039/c7sc04296e

    Figure Lengend Snippet: Simultaneous detection of multiple DNA glycosylases by TIRF-based single-molecule imaging in the absence (A and E) and presence of hOGG1 (B and F), hAAG (C and G) and both hOGG1 and hAAG (D and H). The Cy3 fluorescence signals are shown in green, and the Cy5 fluorescence signals are shown in red. The Cy3-labeled molecular beacon (0.3 μM), the Cy5-labeled molecular beacon (0.3 μM), hOGG1 (0.1 U μL –1 ), hAAG (0.1 U μL –1 ) and APE1 (0.1 U μL –1 ) were used in this research. The scale bar is 5 μm.

    Article Snippet: Then, 1 μL of the products of the molecular beacon (the final concentration of each substrate is 0.3 μM) were added into 20 μL of the reaction solution containing a varied concentration of hOGG1 and/or hAAG, 1× NEBuffer 2, 100 μg mL–1 BSA, 1× NEBuffer 4, 1× ThermoPol buffer and 0.1 U μL–1 APE1, followed by incubation at 37 °C for 1.5 h. The reaction was terminated by heating at 80 °C for 20 min.

    Techniques: Imaging, Fluorescence, Labeling

    Measurement of the Cy3 counts and Cy5 counts in response to the reaction buffer (control), 0.1 g L –1 BSA, 0.1 U μL –1 UDG, 0.1 U μL –1 TDG, 0.1 U μL –1 hOGG1, 0.1 U μL –1 hAAG and 0.1 U μL –1 hOGG1 + 0.1 U μL –1 hAAG. The Cy3-labeled molecular beacon (0.3 μM), Cy5-labeled molecular beacon (0.3 μM) and APE1 (0.1 U μL –1 ) were used in this research. The error bars represent the standard deviations of the three experiments.

    Journal: Chemical Science

    Article Title: Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level †Electronic supplementary information (ESI) available: Molecular mechanism of the DNA glycosylase-mediated cleavage of molecular beacons and optimization of the experimental conditions. See DOI: 10.1039/c7sc04296e

    doi: 10.1039/c7sc04296e

    Figure Lengend Snippet: Measurement of the Cy3 counts and Cy5 counts in response to the reaction buffer (control), 0.1 g L –1 BSA, 0.1 U μL –1 UDG, 0.1 U μL –1 TDG, 0.1 U μL –1 hOGG1, 0.1 U μL –1 hAAG and 0.1 U μL –1 hOGG1 + 0.1 U μL –1 hAAG. The Cy3-labeled molecular beacon (0.3 μM), Cy5-labeled molecular beacon (0.3 μM) and APE1 (0.1 U μL –1 ) were used in this research. The error bars represent the standard deviations of the three experiments.

    Article Snippet: Then, 1 μL of the products of the molecular beacon (the final concentration of each substrate is 0.3 μM) were added into 20 μL of the reaction solution containing a varied concentration of hOGG1 and/or hAAG, 1× NEBuffer 2, 100 μg mL–1 BSA, 1× NEBuffer 4, 1× ThermoPol buffer and 0.1 U μL–1 APE1, followed by incubation at 37 °C for 1.5 h. The reaction was terminated by heating at 80 °C for 20 min.

    Techniques: Labeling

    (A) Measurement of the Cy3 counts generated by different concentrations of hOGG1. The inset shows the linear relationship between the Cy3 counts and the logarithm of the hOGG1 concentration. The Cy3-labeled molecular beacon (0.3 μM) and APE1 (0.1 U μL –1 ) were used in this research. (B) Measurement of the Cy5 counts generated by different concentrations of hAAG. The inset shows the linear relationship between the Cy5 counts and the logarithm of the hAAG concentration. The Cy5-labeled molecular beacon (0.3 μM) and APE1 (0.1 U μL –1 ) were used in this research. The error bars represent the standard deviations of the three experiments.

    Journal: Chemical Science

    Article Title: Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level †Electronic supplementary information (ESI) available: Molecular mechanism of the DNA glycosylase-mediated cleavage of molecular beacons and optimization of the experimental conditions. See DOI: 10.1039/c7sc04296e

    doi: 10.1039/c7sc04296e

    Figure Lengend Snippet: (A) Measurement of the Cy3 counts generated by different concentrations of hOGG1. The inset shows the linear relationship between the Cy3 counts and the logarithm of the hOGG1 concentration. The Cy3-labeled molecular beacon (0.3 μM) and APE1 (0.1 U μL –1 ) were used in this research. (B) Measurement of the Cy5 counts generated by different concentrations of hAAG. The inset shows the linear relationship between the Cy5 counts and the logarithm of the hAAG concentration. The Cy5-labeled molecular beacon (0.3 μM) and APE1 (0.1 U μL –1 ) were used in this research. The error bars represent the standard deviations of the three experiments.

    Article Snippet: Then, 1 μL of the products of the molecular beacon (the final concentration of each substrate is 0.3 μM) were added into 20 μL of the reaction solution containing a varied concentration of hOGG1 and/or hAAG, 1× NEBuffer 2, 100 μg mL–1 BSA, 1× NEBuffer 4, 1× ThermoPol buffer and 0.1 U μL–1 APE1, followed by incubation at 37 °C for 1.5 h. The reaction was terminated by heating at 80 °C for 20 min.

    Techniques: Generated, Concentration Assay, Labeling

    The level of DNA pyrimidine and purine oxidation in HUVECs (analysis by alkaline comet assay using endonuclease III (Nth)) or human 8 oxoguanine DNA glycosylase (hOOG1) was induced by 25-hydroxycholesterol (10 µg/mL) ( A ). The repair of DNA damage in cells was induced by ( B ) dabigatran (100 ng/mL and 500 ng/mL) and ( C ) rivaroxaban (100 ng/mL and 500 ng/mL). Each experiment included a positive control (PC) which concerned the cells incubated with hydrogen peroxide at 20 µM for 15 min on ice and subsequently treated with the enzymes. The value of DNA in the figure captions for comet tail in the presence of either enzyme for all groups was reduced by the value obtained in the comet assay without any enzyme and the value for enzyme buffer only. The number of cells scored from each slide was 100. The mean value for 100 cells analyzed in each treatment in four independent experiments (400 total cells) was recorded. Mean ± SEM was calculated from four individual experiments (400 comets) and was significantly different from negative controls at * p

    Journal: International Journal of Molecular Sciences

    Article Title: The Protective Effect of Dabigatran and Rivaroxaban on DNA Oxidative Changes in a Model of Vascular Endothelial Damage with Oxidized Cholesterol

    doi: 10.3390/ijms21061953

    Figure Lengend Snippet: The level of DNA pyrimidine and purine oxidation in HUVECs (analysis by alkaline comet assay using endonuclease III (Nth)) or human 8 oxoguanine DNA glycosylase (hOOG1) was induced by 25-hydroxycholesterol (10 µg/mL) ( A ). The repair of DNA damage in cells was induced by ( B ) dabigatran (100 ng/mL and 500 ng/mL) and ( C ) rivaroxaban (100 ng/mL and 500 ng/mL). Each experiment included a positive control (PC) which concerned the cells incubated with hydrogen peroxide at 20 µM for 15 min on ice and subsequently treated with the enzymes. The value of DNA in the figure captions for comet tail in the presence of either enzyme for all groups was reduced by the value obtained in the comet assay without any enzyme and the value for enzyme buffer only. The number of cells scored from each slide was 100. The mean value for 100 cells analyzed in each treatment in four independent experiments (400 total cells) was recorded. Mean ± SEM was calculated from four individual experiments (400 comets) and was significantly different from negative controls at * p

    Article Snippet: Endonuclease III (Nth) and human 8-oxoguanine DNA glycosylase (hOGG1) were acquired in New England Biolabs (Ipswich, MA, USA).

    Techniques: Alkaline Single Cell Gel Electrophoresis, Positive Control, Incubation, Single Cell Gel Electrophoresis