hmis12 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Millipore hmis12
    ). (B and C) <t>FLAG-hMis12</t> was immunoprecipitated
    Hmis12, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmis12/product/Millipore
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    hmis12 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    85
    Horizon Discovery hmis12
    The <t>hMis12</t> complex is required for kinetochore fiber formation and kinetochore assembly. (A) After the indicated perturbations, HeLa cells were incubated in ice-cold media for 10 min and stained for tubulin (green), centromeres (ACA, yellow), DNA, and hDsn1. (B) Interkinetochore distances measured in transfected HeLas. Error bars represent SD. (C) After siRNA transfection, HeLa cells were treated with nocodazole and stained for hDsn1 and CENP-E. Insets show boxed sister kinetochore pairs at higher magnification. (D) After the indicated perturbations, HeLa cells were stained for Ndc80, centromeres (ACA), DNA, and hDsn1 (not depicted). (E and F) After the depletion of hDsn1, HeLa cells stably expressing YFP–CENP-A (E) or GFP–CENP-H (F) were stained for hDsn1 and YFP. (D–F) The mean kinetochore fluorescence intensities, which are expressed as percentages relative to controls, are indicated in the bottom right of panels. Bars, 10 μm; (insets), 1 μm.
    Hmis12, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmis12/product/Horizon Discovery
    Average 85 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    hmis12 - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    85
    Abcam rabbit anti hmis12
    The <t>hMis12</t> complex is required for kinetochore fiber formation and kinetochore assembly. (A) After the indicated perturbations, HeLa cells were incubated in ice-cold media for 10 min and stained for tubulin (green), centromeres (ACA, yellow), DNA, and hDsn1. (B) Interkinetochore distances measured in transfected HeLas. Error bars represent SD. (C) After siRNA transfection, HeLa cells were treated with nocodazole and stained for hDsn1 and CENP-E. Insets show boxed sister kinetochore pairs at higher magnification. (D) After the indicated perturbations, HeLa cells were stained for Ndc80, centromeres (ACA), DNA, and hDsn1 (not depicted). (E and F) After the depletion of hDsn1, HeLa cells stably expressing YFP–CENP-A (E) or GFP–CENP-H (F) were stained for hDsn1 and YFP. (D–F) The mean kinetochore fluorescence intensities, which are expressed as percentages relative to controls, are indicated in the bottom right of panels. Bars, 10 μm; (insets), 1 μm.
    Rabbit Anti Hmis12, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti hmis12/product/Abcam
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit anti hmis12 - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    85
    Bethyl rabbit anti mis12 antibody affinity purified
    The <t>hMis12</t> complex is required for kinetochore fiber formation and kinetochore assembly. (A) After the indicated perturbations, HeLa cells were incubated in ice-cold media for 10 min and stained for tubulin (green), centromeres (ACA, yellow), DNA, and hDsn1. (B) Interkinetochore distances measured in transfected HeLas. Error bars represent SD. (C) After siRNA transfection, HeLa cells were treated with nocodazole and stained for hDsn1 and CENP-E. Insets show boxed sister kinetochore pairs at higher magnification. (D) After the indicated perturbations, HeLa cells were stained for Ndc80, centromeres (ACA), DNA, and hDsn1 (not depicted). (E and F) After the depletion of hDsn1, HeLa cells stably expressing YFP–CENP-A (E) or GFP–CENP-H (F) were stained for hDsn1 and YFP. (D–F) The mean kinetochore fluorescence intensities, which are expressed as percentages relative to controls, are indicated in the bottom right of panels. Bars, 10 μm; (insets), 1 μm.
    Rabbit Anti Mis12 Antibody Affinity Purified, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mis12 antibody affinity purified/product/Bethyl
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mis12 antibody affinity purified - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    Image Search Results


    ). (B and C) FLAG-hMis12 was immunoprecipitated

    Journal: Molecular and Cellular Biology

    Article Title: Protein Interaction Domain Mapping of Human Kinetochore Protein Blinkin Reveals a Consensus Motif for Binding of Spindle Assembly Checkpoint Proteins Bub1 and BubR1 ▿

    doi: 10.1128/MCB.00815-10

    Figure Lengend Snippet: ). (B and C) FLAG-hMis12 was immunoprecipitated

    Article Snippet: Immunoblots and immunofluorescence experiments were performed using the following antibodies: hMis12 (anti-rabbit Ig, 1:30) , hMis13 (anti-rabbit Ig, 1:1,000) , hMis14 (anti-mouse Ig, 1:20) , hNnf1 (anti-mouse Ig, 1:20; A2), HP1α (anti-mouse Ig, 1:500; Millipore), CENP-A (mouse, 1:100), CENP-C (anti-guinea pig Ig, 1:1,000), blinkin (anti-mouse 31F2, 1:20) ( ) and blinkin(M) (anti-rabbit C5, 1:1,000), Zwint-1 (anti-mouse Ig, 1:20) , Bub1 (anti-sheep Ig, 1:1,000), BubR1 (anti-sheep Ig, 1:1,000) , anti-histone H3S10ph (anti-rabbit Ig, 1:1,000, catalog number ab5176; Abcam), GFP (anti-mouse Ig, 1:500; Roche), and tubulin (anti-mouse Ig, 1:500, DM1A; Sigma).

    Techniques: Immunoprecipitation

    The hMis12 complex is required for kinetochore fiber formation and kinetochore assembly. (A) After the indicated perturbations, HeLa cells were incubated in ice-cold media for 10 min and stained for tubulin (green), centromeres (ACA, yellow), DNA, and hDsn1. (B) Interkinetochore distances measured in transfected HeLas. Error bars represent SD. (C) After siRNA transfection, HeLa cells were treated with nocodazole and stained for hDsn1 and CENP-E. Insets show boxed sister kinetochore pairs at higher magnification. (D) After the indicated perturbations, HeLa cells were stained for Ndc80, centromeres (ACA), DNA, and hDsn1 (not depicted). (E and F) After the depletion of hDsn1, HeLa cells stably expressing YFP–CENP-A (E) or GFP–CENP-H (F) were stained for hDsn1 and YFP. (D–F) The mean kinetochore fluorescence intensities, which are expressed as percentages relative to controls, are indicated in the bottom right of panels. Bars, 10 μm; (insets), 1 μm.

    Journal: The Journal of Cell Biology

    Article Title: The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation

    doi: 10.1083/jcb.200509158

    Figure Lengend Snippet: The hMis12 complex is required for kinetochore fiber formation and kinetochore assembly. (A) After the indicated perturbations, HeLa cells were incubated in ice-cold media for 10 min and stained for tubulin (green), centromeres (ACA, yellow), DNA, and hDsn1. (B) Interkinetochore distances measured in transfected HeLas. Error bars represent SD. (C) After siRNA transfection, HeLa cells were treated with nocodazole and stained for hDsn1 and CENP-E. Insets show boxed sister kinetochore pairs at higher magnification. (D) After the indicated perturbations, HeLa cells were stained for Ndc80, centromeres (ACA), DNA, and hDsn1 (not depicted). (E and F) After the depletion of hDsn1, HeLa cells stably expressing YFP–CENP-A (E) or GFP–CENP-H (F) were stained for hDsn1 and YFP. (D–F) The mean kinetochore fluorescence intensities, which are expressed as percentages relative to controls, are indicated in the bottom right of panels. Bars, 10 μm; (insets), 1 μm.

    Article Snippet: Predesigned siRNAs targeting hNsl1DC31 (Ambion), hDsn1Q9H410 , hNnf1PMF1 , hMis12 (Dharmacon), hNuf2 (a gift from J. DeLuca, University of North Carolina, Chapel Hill, NC; ), or nonspecific control siRNAs (Dharmacon) were transfected according to manufacturer's directions using Oligofectamine and serum-free OptiMEM (Invitrogen).

    Techniques: Incubation, Staining, Transfection, Stable Transfection, Expressing, Fluorescence

    The hMis12 complex is required for chromosome biorientation. (A) HeLa cells stably expressing YFP–CENP-A were imaged after transfection of the indicated siRNAs; time is given in minutes. Paired sister kinetochores were pseudocolored to illustrate their dynamics. (B) Distance of pseudocolored sister kinetochores in A to the metaphase plate over time for the control cell (left) and hMis12-depleted cell (right). Arrowheads on the x axis indicate the time of the panels in A. Bars, 5 μm.

    Journal: The Journal of Cell Biology

    Article Title: The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation

    doi: 10.1083/jcb.200509158

    Figure Lengend Snippet: The hMis12 complex is required for chromosome biorientation. (A) HeLa cells stably expressing YFP–CENP-A were imaged after transfection of the indicated siRNAs; time is given in minutes. Paired sister kinetochores were pseudocolored to illustrate their dynamics. (B) Distance of pseudocolored sister kinetochores in A to the metaphase plate over time for the control cell (left) and hMis12-depleted cell (right). Arrowheads on the x axis indicate the time of the panels in A. Bars, 5 μm.

    Article Snippet: Predesigned siRNAs targeting hNsl1DC31 (Ambion), hDsn1Q9H410 , hNnf1PMF1 , hMis12 (Dharmacon), hNuf2 (a gift from J. DeLuca, University of North Carolina, Chapel Hill, NC; ), or nonspecific control siRNAs (Dharmacon) were transfected according to manufacturer's directions using Oligofectamine and serum-free OptiMEM (Invitrogen).

    Techniques: Stable Transfection, Expressing, Transfection

    The hMis12 complex is required for chromosome alignment and segregation. (A) After transfection of the indicated siRNAs, HeLa cells stably expressing YFP–CENP-A were stained for tubulin (green), DNA (blue), and CENP-A. (B) Chicken DT40 cells were stained for tubulin (green) and DNA (blue) at 0 (control) or 30 h (knockdown) after tet addition. (C) HeLa cells stably expressing YFP-histone H2B were imaged by time-lapse fluorescence microscopy after transfection of the indicated siRNAs; time is given in hours/minutes. (D) Summary of live imaging analysis in HeLa cells. (E) After siRNA transfection, HeLa cells were treated with nocodazole (NOC) and stained for hDsn1 and BubR1. Values indicating the mean kinetochore fluorescence intensities are given relative to controls. Bars, 10 μm.

    Journal: The Journal of Cell Biology

    Article Title: The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation

    doi: 10.1083/jcb.200509158

    Figure Lengend Snippet: The hMis12 complex is required for chromosome alignment and segregation. (A) After transfection of the indicated siRNAs, HeLa cells stably expressing YFP–CENP-A were stained for tubulin (green), DNA (blue), and CENP-A. (B) Chicken DT40 cells were stained for tubulin (green) and DNA (blue) at 0 (control) or 30 h (knockdown) after tet addition. (C) HeLa cells stably expressing YFP-histone H2B were imaged by time-lapse fluorescence microscopy after transfection of the indicated siRNAs; time is given in hours/minutes. (D) Summary of live imaging analysis in HeLa cells. (E) After siRNA transfection, HeLa cells were treated with nocodazole (NOC) and stained for hDsn1 and BubR1. Values indicating the mean kinetochore fluorescence intensities are given relative to controls. Bars, 10 μm.

    Article Snippet: Predesigned siRNAs targeting hNsl1DC31 (Ambion), hDsn1Q9H410 , hNnf1PMF1 , hMis12 (Dharmacon), hNuf2 (a gift from J. DeLuca, University of North Carolina, Chapel Hill, NC; ), or nonspecific control siRNAs (Dharmacon) were transfected according to manufacturer's directions using Oligofectamine and serum-free OptiMEM (Invitrogen).

    Techniques: Transfection, Stable Transfection, Expressing, Staining, Fluorescence, Microscopy, Imaging

    hMis12, hDsn1, hNnf1, and hNsl1 form a discrete complex. (A) Immunoblots of mitotic HeLa extracts fractionated on a Superose 6 gel filtration column at 100 or 600 mM KCl. Stokes radii of standards: thyroglobulin (8.5 nm), ferritin (6.1 nm), catalase (5.2 nm), aldolase (4.8 nm), and ovalbumin (3.1 nm). (B) Coexpression of hMis12, PMF1, DC31, and Q9H410-6xHis using a polycistronic system in bacteria. Coomassie-stained peak fractions are shown. (C) Migration of the recombinant complex on a Superose 6 gel filtration column. (D) Comigration of the purified recombinant complex and the endogenous complex on a 5–20% sucrose gradient. PMF1 blots are shown for brevity. Standards are BSA (4.3 S), aldolase (7.35 S), and catalase (11.3 S).

    Journal: The Journal of Cell Biology

    Article Title: The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation

    doi: 10.1083/jcb.200509158

    Figure Lengend Snippet: hMis12, hDsn1, hNnf1, and hNsl1 form a discrete complex. (A) Immunoblots of mitotic HeLa extracts fractionated on a Superose 6 gel filtration column at 100 or 600 mM KCl. Stokes radii of standards: thyroglobulin (8.5 nm), ferritin (6.1 nm), catalase (5.2 nm), aldolase (4.8 nm), and ovalbumin (3.1 nm). (B) Coexpression of hMis12, PMF1, DC31, and Q9H410-6xHis using a polycistronic system in bacteria. Coomassie-stained peak fractions are shown. (C) Migration of the recombinant complex on a Superose 6 gel filtration column. (D) Comigration of the purified recombinant complex and the endogenous complex on a 5–20% sucrose gradient. PMF1 blots are shown for brevity. Standards are BSA (4.3 S), aldolase (7.35 S), and catalase (11.3 S).

    Article Snippet: Predesigned siRNAs targeting hNsl1DC31 (Ambion), hDsn1Q9H410 , hNnf1PMF1 , hMis12 (Dharmacon), hNuf2 (a gift from J. DeLuca, University of North Carolina, Chapel Hill, NC; ), or nonspecific control siRNAs (Dharmacon) were transfected according to manufacturer's directions using Oligofectamine and serum-free OptiMEM (Invitrogen).

    Techniques: Western Blot, Filtration, Staining, Migration, Recombinant, Purification

    Kinetochore localization of the hMis12 complex is interdependent. (A) HeLa cells stained for CENP-A (red), hDsn1 (green), and Ndc80 (blue). Insets show higher magnification of the boxed pair of sister kinetochores. (B) Corresponding fluorescence intensity profiles in a line scan. (C) HeLa cells were transfected with the indicated siRNAs and stained for DNA, centromeres (ACA), and the indicated proteins. (D) HeLa cells were harvested after the transfection of siRNAs, and serial dilutions of control and depleted extracts were immunoblotted for hDsn1 and hNnf1. α-Tubulin was used as a loading control. (E) Chicken Mis12 loss-of-function mutant cells stably expressing Dsn1-GFP, Nsl1-GFP, and Nnf1-GFP constructs were fixed and analyzed at 0 (control) and 30 h (knockdown) after the addition of tet. Bars (A), 1 μm; (C and E), 10 μm.

    Journal: The Journal of Cell Biology

    Article Title: The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation

    doi: 10.1083/jcb.200509158

    Figure Lengend Snippet: Kinetochore localization of the hMis12 complex is interdependent. (A) HeLa cells stained for CENP-A (red), hDsn1 (green), and Ndc80 (blue). Insets show higher magnification of the boxed pair of sister kinetochores. (B) Corresponding fluorescence intensity profiles in a line scan. (C) HeLa cells were transfected with the indicated siRNAs and stained for DNA, centromeres (ACA), and the indicated proteins. (D) HeLa cells were harvested after the transfection of siRNAs, and serial dilutions of control and depleted extracts were immunoblotted for hDsn1 and hNnf1. α-Tubulin was used as a loading control. (E) Chicken Mis12 loss-of-function mutant cells stably expressing Dsn1-GFP, Nsl1-GFP, and Nnf1-GFP constructs were fixed and analyzed at 0 (control) and 30 h (knockdown) after the addition of tet. Bars (A), 1 μm; (C and E), 10 μm.

    Article Snippet: Predesigned siRNAs targeting hNsl1DC31 (Ambion), hDsn1Q9H410 , hNnf1PMF1 , hMis12 (Dharmacon), hNuf2 (a gift from J. DeLuca, University of North Carolina, Chapel Hill, NC; ), or nonspecific control siRNAs (Dharmacon) were transfected according to manufacturer's directions using Oligofectamine and serum-free OptiMEM (Invitrogen).

    Techniques: Staining, Fluorescence, Transfection, Mutagenesis, Stable Transfection, Expressing, Construct