hiseq 2500 Search Results


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  • 94
    Macrogen hiseq 2500
    Hiseq 2500, supplied by Macrogen, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500/product/Macrogen
    Average 94 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 - by Bioz Stars, 2020-07
    94/100 stars
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    92
    Solexa hiseq 2500
    Hiseq 2500, supplied by Solexa, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500/product/Solexa
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 - by Bioz Stars, 2020-07
    92/100 stars
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    99
    Illumina Inc hiseq 2500
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11761 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500/product/Illumina Inc
    Average 99 stars, based on 11761 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 - by Bioz Stars, 2020-07
    99/100 stars
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    93
    Novogene hiseq 2500 pe250
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 Pe250, supplied by Novogene, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 pe250/product/Novogene
    Average 93 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 pe250 - by Bioz Stars, 2020-07
    93/100 stars
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    92
    GATC Biotech hiseq 2500 system
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 System, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 system/product/GATC Biotech
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 system - by Bioz Stars, 2020-07
    92/100 stars
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    92
    Elim Bio hiseq 2500 instrument
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 Instrument, supplied by Elim Bio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 instrument/product/Elim Bio
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 instrument - by Bioz Stars, 2020-07
    92/100 stars
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    93
    Biomarker Technologies Co illumina hiseq 2500
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Illumina Hiseq 2500, supplied by Biomarker Technologies Co, used in various techniques. Bioz Stars score: 93/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2500/product/Biomarker Technologies Co
    Average 93 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2500 - by Bioz Stars, 2020-07
    93/100 stars
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    88
    Illumina Inc hiseq 2000 2500
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2000 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 2500/product/Illumina Inc
    Average 88 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 2500 - by Bioz Stars, 2020-07
    88/100 stars
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    92
    Novogene hiseq 2500 platform
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 Platform, supplied by Novogene, used in various techniques. Bioz Stars score: 92/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 platform/product/Novogene
    Average 92 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 platform - by Bioz Stars, 2020-07
    92/100 stars
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    91
    GATC Biotech illumina hiseq 2500
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Illumina Hiseq 2500, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2500/product/GATC Biotech
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2500 - by Bioz Stars, 2020-07
    91/100 stars
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    92
    Biomarker Technologies Co hiseq 2500 system
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 System, supplied by Biomarker Technologies Co, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 system/product/Biomarker Technologies Co
    Average 92 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 system - by Bioz Stars, 2020-07
    92/100 stars
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    93
    Fasteris Life hiseq 2500 platform
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 Platform, supplied by Fasteris Life, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 platform/product/Fasteris Life
    Average 93 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 platform - by Bioz Stars, 2020-07
    93/100 stars
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    92
    ChunLab hiseq 2500 platform
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 Platform, supplied by ChunLab, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 platform/product/ChunLab
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 platform - by Bioz Stars, 2020-07
    92/100 stars
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    92
    Bionova hiseq 2500 system
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 System, supplied by Bionova, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 system/product/Bionova
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 system - by Bioz Stars, 2020-07
    92/100 stars
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    91
    Bionova hiseq 2500 platform
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 Platform, supplied by Bionova, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 platform/product/Bionova
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 platform - by Bioz Stars, 2020-07
    91/100 stars
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    93
    Capital Biosciences hiseq 2500 platform
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 Platform, supplied by Capital Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 platform/product/Capital Biosciences
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 platform - by Bioz Stars, 2020-07
    93/100 stars
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    90
    Illumina Inc hisequation 2500
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hisequation 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hisequation 2500/product/Illumina Inc
    Average 90 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    hisequation 2500 - by Bioz Stars, 2020-07
    90/100 stars
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    93
    Genewiz hiseq 2500 system
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    Hiseq 2500 System, supplied by Genewiz, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 system/product/Genewiz
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 system - by Bioz Stars, 2020-07
    93/100 stars
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    93
    Illumina Inc 50se hiseq 2500
    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.
    50se Hiseq 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/50se hiseq 2500/product/Illumina Inc
    Average 93 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    50se hiseq 2500 - by Bioz Stars, 2020-07
    93/100 stars
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    90
    Pacific Biosciences illumina hiseq 2500
    Effect of the GC content and ERCC length on the estimation of the ERCC cDNA abundance with the ONT MinION platform. The figures present deviations of the ERCC expression level estimates with the ONT MinION platform from the Ambion RNA molecular counts ( A,C ) or from the <t>Illumina</t> HiSeq 2500/MiSeq estimated cDNA abundance ( B,D ) as a function of the GC content ( A,B ) and the ERCC length ( C,D ). We plot the log2 ratio of observed (ONT MinION) to expected (Ambion, Illumina) read counts for the ERCC spike-ins (y-axis, log) for each of the samples relative to their length or GC content (x-axis). Due to the variable sequencing depth from each ERCC MinION experiment, each point is the average value from different MinION flow cell runs if at least 5 reads have been detected for this point in the corresponding MinION runs. The points are colored differently based on the number of flow cell runs in which they were detected (red, green, cyan, purple correspond to values derived from one, two, three or four flow cell runs respectively). The standard deviation is also presented for points with values from two or more MinION runs.
    Illumina Hiseq 2500, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2500/product/Pacific Biosciences
    Average 90 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2500 - by Bioz Stars, 2020-07
    90/100 stars
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    91
    Eurofins illumina hiseq 2500
    Effect of the GC content and ERCC length on the estimation of the ERCC cDNA abundance with the ONT MinION platform. The figures present deviations of the ERCC expression level estimates with the ONT MinION platform from the Ambion RNA molecular counts ( A,C ) or from the <t>Illumina</t> HiSeq 2500/MiSeq estimated cDNA abundance ( B,D ) as a function of the GC content ( A,B ) and the ERCC length ( C,D ). We plot the log2 ratio of observed (ONT MinION) to expected (Ambion, Illumina) read counts for the ERCC spike-ins (y-axis, log) for each of the samples relative to their length or GC content (x-axis). Due to the variable sequencing depth from each ERCC MinION experiment, each point is the average value from different MinION flow cell runs if at least 5 reads have been detected for this point in the corresponding MinION runs. The points are colored differently based on the number of flow cell runs in which they were detected (red, green, cyan, purple correspond to values derived from one, two, three or four flow cell runs respectively). The standard deviation is also presented for points with values from two or more MinION runs.
    Illumina Hiseq 2500, supplied by Eurofins, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2500/product/Eurofins
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2500 - by Bioz Stars, 2020-07
    91/100 stars
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    Image Search Results


    (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that  LacZ  was a clear cluster that was included in all fluorescent liposomes and with no other common factors.

    Journal: Scientific Reports

    Article Title: Integrating reductive and synthetic approaches in biology using man-made cell-like compartments

    doi: 10.1038/srep04722

    Figure Lengend Snippet: (a) Hierarchical cluster analysis for genes included in non-fluorescent liposomes. Ten non-fluorescent liposomes that did not show β-galactoside hydrolysis activity were isolated, and the genes included in these liposomes were determined by multiplex next-generation sequencing using HiSeq 2500. In the hierarchical cluster analysis for these genes, there was no commonality among the genes included in each liposome, which indicated a random pattern. Each row represents a separate gene, each column represents a separate liposome and genes found in each liposome are shown in red. (b) Hierarchical cluster analysis for the genes included in fluorescent liposomes. Hierarchical cluster analysis revealed that LacZ was a clear cluster that was included in all fluorescent liposomes and with no other common factors.

    Article Snippet: Next, the liposome-derived DNA fragments and the E. coli ORF library were pre-treated for HiSeq 2500 according to the Nextera XT DNA preparation kit protocol (Illumina).

    Techniques: Activity Assay, Isolation, Multiplex Assay, Next-Generation Sequencing

    Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the Nextera XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.

    Journal: Jala (Charlottesville, Va.)

    Article Title: Miniaturization Technologies for Efficient Single-Cell Library Preparation for Next-Generation Sequencing

    doi: 10.1177/2211068216630741

    Figure Lengend Snippet: Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the Nextera XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.

    Article Snippet: Sequencing The 48 pooled libraries were sequenced on one lane of an Illumina HiSeq 2500 at an average total read depth of 5.6 million reads per sample (ST3).

    Techniques: Polymerase Chain Reaction, Amplification, In Vitro, Concentration Assay

    Effect of the GC content and ERCC length on the estimation of the ERCC cDNA abundance with the ONT MinION platform. The figures present deviations of the ERCC expression level estimates with the ONT MinION platform from the Ambion RNA molecular counts ( A,C ) or from the Illumina HiSeq 2500/MiSeq estimated cDNA abundance ( B,D ) as a function of the GC content ( A,B ) and the ERCC length ( C,D ). We plot the log2 ratio of observed (ONT MinION) to expected (Ambion, Illumina) read counts for the ERCC spike-ins (y-axis, log) for each of the samples relative to their length or GC content (x-axis). Due to the variable sequencing depth from each ERCC MinION experiment, each point is the average value from different MinION flow cell runs if at least 5 reads have been detected for this point in the corresponding MinION runs. The points are colored differently based on the number of flow cell runs in which they were detected (red, green, cyan, purple correspond to values derived from one, two, three or four flow cell runs respectively). The standard deviation is also presented for points with values from two or more MinION runs.

    Journal: Scientific Reports

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

    doi: 10.1038/srep31602

    Figure Lengend Snippet: Effect of the GC content and ERCC length on the estimation of the ERCC cDNA abundance with the ONT MinION platform. The figures present deviations of the ERCC expression level estimates with the ONT MinION platform from the Ambion RNA molecular counts ( A,C ) or from the Illumina HiSeq 2500/MiSeq estimated cDNA abundance ( B,D ) as a function of the GC content ( A,B ) and the ERCC length ( C,D ). We plot the log2 ratio of observed (ONT MinION) to expected (Ambion, Illumina) read counts for the ERCC spike-ins (y-axis, log) for each of the samples relative to their length or GC content (x-axis). Due to the variable sequencing depth from each ERCC MinION experiment, each point is the average value from different MinION flow cell runs if at least 5 reads have been detected for this point in the corresponding MinION runs. The points are colored differently based on the number of flow cell runs in which they were detected (red, green, cyan, purple correspond to values derived from one, two, three or four flow cell runs respectively). The standard deviation is also presented for points with values from two or more MinION runs.

    Article Snippet: Additionally, a complex cDNA population derived from a human HEK-293 cell line was sequenced on an Illumina HiSeq 2500, PacBio RS II and ONT MinION platforms.

    Techniques: Expressing, Sequencing, Flow Cytometry, Derivative Assay, Standard Deviation

    Estimation of the ERCC cDNA abundance with the ONT MinION platform. We compared the ERCC cDNA abundance estimated from the ONT MinION ( A ) and the Illumina HiSeq 2500 or MiSeq platforms ( B ) against the expected number of RNA molecules as provided from the manufacturer (Ambion). The template reads from the ERCC MinION experiments number 1, 2, 3, 4 were pooled together and used for ( A ). Similarly, the corresponding Illumina data were pooled together for ( B ). The Illumina molecular counts data were derived using 5′ molecular tags at the RT step as described in material and methods. The total number of molecules presented on the x-axis corresponds to 3.5 pgs of ERCC RNA. In both axes the log10 transformation of the original count number is used.

    Journal: Scientific Reports

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

    doi: 10.1038/srep31602

    Figure Lengend Snippet: Estimation of the ERCC cDNA abundance with the ONT MinION platform. We compared the ERCC cDNA abundance estimated from the ONT MinION ( A ) and the Illumina HiSeq 2500 or MiSeq platforms ( B ) against the expected number of RNA molecules as provided from the manufacturer (Ambion). The template reads from the ERCC MinION experiments number 1, 2, 3, 4 were pooled together and used for ( A ). Similarly, the corresponding Illumina data were pooled together for ( B ). The Illumina molecular counts data were derived using 5′ molecular tags at the RT step as described in material and methods. The total number of molecules presented on the x-axis corresponds to 3.5 pgs of ERCC RNA. In both axes the log10 transformation of the original count number is used.

    Article Snippet: Additionally, a complex cDNA population derived from a human HEK-293 cell line was sequenced on an Illumina HiSeq 2500, PacBio RS II and ONT MinION platforms.

    Techniques: Derivative Assay, Transformation Assay

    Detection of different cDNA species for the RPL41 gene from either the Illumina HiSeq 2500 platform, the ONT MinION platform or the PacBio RS II platform. ONT MinION reads that were sequenced as full length, as defined by the presence of both the 5′ and 3′ RT adaptors, are presented. For the PacBio RS II example the corresponding “Circular Consensus Sequencing” reads are presented. These reads correspond to fully sequenced molecules. mRNA molecules from GenBank for the RPL41 gene are also shown. For the Illumina data a pileup of the sequenced paired-end fragments is presented.

    Journal: Scientific Reports

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

    doi: 10.1038/srep31602

    Figure Lengend Snippet: Detection of different cDNA species for the RPL41 gene from either the Illumina HiSeq 2500 platform, the ONT MinION platform or the PacBio RS II platform. ONT MinION reads that were sequenced as full length, as defined by the presence of both the 5′ and 3′ RT adaptors, are presented. For the PacBio RS II example the corresponding “Circular Consensus Sequencing” reads are presented. These reads correspond to fully sequenced molecules. mRNA molecules from GenBank for the RPL41 gene are also shown. For the Illumina data a pileup of the sequenced paired-end fragments is presented.

    Article Snippet: Additionally, a complex cDNA population derived from a human HEK-293 cell line was sequenced on an Illumina HiSeq 2500, PacBio RS II and ONT MinION platforms.

    Techniques: Sequencing

    Estimation of the HEK-293 cDNA isoform abundance with three sequencing platforms. The comparison between the cDNA isoform abundance estimated from the Illumina HiSeq 2500 platform and from the PacBio RS II platform is presented in ( A ). The expression level of the HEK-293 isoforms estimated with the ONT MinION platform is compared with the one calculated from either the PacBio RS II ( B ) or the Illumina HiSeq 2500 platform ( C ). For the Illumina HiSeq 2500 platform the expression level, presented as TPM, was estimated with the Sailfish 21 software. For the PacBio RS II or the ONT MinION platform the counts of sequenced molecules per isoform are presented.

    Journal: Scientific Reports

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

    doi: 10.1038/srep31602

    Figure Lengend Snippet: Estimation of the HEK-293 cDNA isoform abundance with three sequencing platforms. The comparison between the cDNA isoform abundance estimated from the Illumina HiSeq 2500 platform and from the PacBio RS II platform is presented in ( A ). The expression level of the HEK-293 isoforms estimated with the ONT MinION platform is compared with the one calculated from either the PacBio RS II ( B ) or the Illumina HiSeq 2500 platform ( C ). For the Illumina HiSeq 2500 platform the expression level, presented as TPM, was estimated with the Sailfish 21 software. For the PacBio RS II or the ONT MinION platform the counts of sequenced molecules per isoform are presented.

    Article Snippet: Additionally, a complex cDNA population derived from a human HEK-293 cell line was sequenced on an Illumina HiSeq 2500, PacBio RS II and ONT MinION platforms.

    Techniques: Sequencing, Expressing, Software