hiseq 2000 platform Illumina Inc Search Results


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  • 99
    Illumina Inc hiseq 2000 platform
    Validation of RNA-Seq data by quantitative real-time PCR (qRT-PCR).  (A)  Comparison of the expressions profile of eight DEGs determined by Illumina HiSeq™ 2000 sequencing platform and qRT-PCR at 48 h (pi) using ribosomal protein subunit S4 ( rps4 ) as housekeeping gene. Data shown are the mean of triplicates ± SD.  (B)  Correlation of data between RNA-Seq and qRT-PCR techniques.
    Hiseq 2000 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 20605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 20605 article reviews
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    99
    Illumina Inc hiseq 2000 sequencing platform
    Effect of the integration procedures on TCGA LUSC datasets. Two datasets comprising of Illumina <t>HiSeq-2000</t> and Affymetrix HG-U133A data were employed to evaluate the effect of the integration on two different platforms. After applying the integration procedure, DEGs were computed varying the p-value threshold. Receiver-Operating Characteristic (ROC) curves were computed using known DEGs, and the performances were assessed by means of Area Under the ROC Curve (AUC)
    Hiseq 2000 Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1626 article reviews
    Price from $9.99 to $1999.99
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    96
    Illumina Inc hiseq 2000 2500 platform
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 2500 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 429 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 429 article reviews
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    86
    Illumina Inc hiseq 2000 paired end platform
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 Paired End Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc hiseq sequencing platform
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 859 article reviews
    Price from $9.99 to $1999.99
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    86
    Illumina Inc hiseq 2000 sequencer platform
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 Sequencer Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Illumina Inc hiseq 2000 genome analyzer platform
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 Genome Analyzer Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Illumina Inc ss hs20
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Ss Hs20, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Illumina Inc gpl11154 platform
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Gpl11154 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Illumina Inc hiseq 2000 microrna sequencing platforms
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 Microrna Sequencing Platforms, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Illumina Inc hiseq 2000 2500 platforms
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 2500 Platforms, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 21 article reviews
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    91
    Illumina Inc hiseq 2000 4000 platforms
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 4000 Platforms, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Illumina Inc hiseq 2000 miseq sequencing platform
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 Miseq Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 miseq sequencing platform/product/Illumina Inc
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    Image Search Results


    Validation of RNA-Seq data by quantitative real-time PCR (qRT-PCR).  (A)  Comparison of the expressions profile of eight DEGs determined by Illumina HiSeq™ 2000 sequencing platform and qRT-PCR at 48 h (pi) using ribosomal protein subunit S4 ( rps4 ) as housekeeping gene. Data shown are the mean of triplicates ± SD.  (B)  Correlation of data between RNA-Seq and qRT-PCR techniques.

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic Profiles of Senegalese Sole Infected With Nervous Necrosis Virus Reassortants Presenting Different Degree of Virulence

    doi: 10.3389/fimmu.2018.01626

    Figure Lengend Snippet: Validation of RNA-Seq data by quantitative real-time PCR (qRT-PCR). (A) Comparison of the expressions profile of eight DEGs determined by Illumina HiSeq™ 2000 sequencing platform and qRT-PCR at 48 h (pi) using ribosomal protein subunit S4 ( rps4 ) as housekeeping gene. Data shown are the mean of triplicates ± SD. (B) Correlation of data between RNA-Seq and qRT-PCR techniques.

    Article Snippet: Therefore, samples from 48 h p.i. were selected for RNA-Seq analysis (threefold replicated), being sequenced using Illumina HiSeq™ 2000 platform.

    Techniques: RNA Sequencing Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Sequencing

    Comparison of relative species abundance between BGISEQ-500 and HiSeq 2000. Averaged microbial abundance calculated with Metaphlan2 across BGI replicates plotted against microbial abundance for the corresponding Illumina replicates for all samples. Species are colored by GC content.

    Journal: GigaScience

    Article Title: Assessment of the cPAS-based BGISEQ-500 platform for metagenomic sequencing

    doi: 10.1093/gigascience/gix133

    Figure Lengend Snippet: Comparison of relative species abundance between BGISEQ-500 and HiSeq 2000. Averaged microbial abundance calculated with Metaphlan2 across BGI replicates plotted against microbial abundance for the corresponding Illumina replicates for all samples. Species are colored by GC content.

    Article Snippet: To further document the quantitative consistency and performance regarding GC content observed for the BGISEQ-500 and HiSeq 2000 platforms, the same 20 DNA samples were processed to construct libraries and sequenced on an Illumina HiSeq 4000 platform using the HiSeq 3000/4000SBS Kit (300 cycles) for 100 bp of paired-end reads.

    Techniques:

    Evaluation of inter-platform consistency. For 19 cross-platform replicates at 99% CI, 91.89% genes in the BGISEQ-500 datasets showed the expected mapped read count fluctuations using HiSeq 2000 (A). The Spearman correlation analyses revealed high agreement within 19 pair of platform replicates between BGISEQ-500 and HiSeq 2000 (B) (an average Spearman's rho of 0.724 at gene level [top] and 0.948 at species level [bottom]) and between BGISEQ-500 and HiSeq 4000 (C) (an average Spearman's rho of 0.859 at gene level [top] and 0.965 at species level [bottom]).

    Journal: GigaScience

    Article Title: Assessment of the cPAS-based BGISEQ-500 platform for metagenomic sequencing

    doi: 10.1093/gigascience/gix133

    Figure Lengend Snippet: Evaluation of inter-platform consistency. For 19 cross-platform replicates at 99% CI, 91.89% genes in the BGISEQ-500 datasets showed the expected mapped read count fluctuations using HiSeq 2000 (A). The Spearman correlation analyses revealed high agreement within 19 pair of platform replicates between BGISEQ-500 and HiSeq 2000 (B) (an average Spearman's rho of 0.724 at gene level [top] and 0.948 at species level [bottom]) and between BGISEQ-500 and HiSeq 4000 (C) (an average Spearman's rho of 0.859 at gene level [top] and 0.965 at species level [bottom]).

    Article Snippet: To further document the quantitative consistency and performance regarding GC content observed for the BGISEQ-500 and HiSeq 2000 platforms, the same 20 DNA samples were processed to construct libraries and sequenced on an Illumina HiSeq 4000 platform using the HiSeq 3000/4000SBS Kit (300 cycles) for 100 bp of paired-end reads.

    Techniques:

    A, GC content distributions of genes that differed significantly in abundance between platforms. Density curves showing a comparison of GC content distributions of the total 9.9 million IGC genes (blue), all 349 479 highly reproducible (HR) genes (green), and all 11 350 genes that differed significantly in abundance between the 2 platforms (red line). B, Two-dimensional plot showing the GC content distribution of genes that differed significantly in abundance between the BGISEQ-500 and HiSeq 2000 platforms. The x-axis indicates the GC content of genes, the y-axis indicates fold-changes of gene relative abundance (RA), which is calculated by log10 transformed mean RA in the HiSeq 2000 datasets/mean RA in the BGISEQ-500 datasets. C, D, Density histograms showing the coefficients of a robust linear model for relative abundance of genes from the top 20 species and their GC content for genes that differed significantly in abundance between the 2 platforms (C) and for all HR genes (D). D, E, Density curves (E) and 2-dimensional plot (F) showing the GC content distributions of HR genes that differed significantly in abundance between the BGISEQ-500 and Hiseq 4000 platforms.

    Journal: GigaScience

    Article Title: Assessment of the cPAS-based BGISEQ-500 platform for metagenomic sequencing

    doi: 10.1093/gigascience/gix133

    Figure Lengend Snippet: A, GC content distributions of genes that differed significantly in abundance between platforms. Density curves showing a comparison of GC content distributions of the total 9.9 million IGC genes (blue), all 349 479 highly reproducible (HR) genes (green), and all 11 350 genes that differed significantly in abundance between the 2 platforms (red line). B, Two-dimensional plot showing the GC content distribution of genes that differed significantly in abundance between the BGISEQ-500 and HiSeq 2000 platforms. The x-axis indicates the GC content of genes, the y-axis indicates fold-changes of gene relative abundance (RA), which is calculated by log10 transformed mean RA in the HiSeq 2000 datasets/mean RA in the BGISEQ-500 datasets. C, D, Density histograms showing the coefficients of a robust linear model for relative abundance of genes from the top 20 species and their GC content for genes that differed significantly in abundance between the 2 platforms (C) and for all HR genes (D). D, E, Density curves (E) and 2-dimensional plot (F) showing the GC content distributions of HR genes that differed significantly in abundance between the BGISEQ-500 and Hiseq 4000 platforms.

    Article Snippet: To further document the quantitative consistency and performance regarding GC content observed for the BGISEQ-500 and HiSeq 2000 platforms, the same 20 DNA samples were processed to construct libraries and sequenced on an Illumina HiSeq 4000 platform using the HiSeq 3000/4000SBS Kit (300 cycles) for 100 bp of paired-end reads.

    Techniques: Transformation Assay

    Schematic model summarizing the study design and analysis strategy. Schematic diagram depicting the process of data generation, including collection of fecal samples and extraction of DNA from 20 healthy subjects, library preparation, and sequencing strategy for BGISEQ-500 and HiSeq 2000. Each circle indicates 1 independent subject, with subject ID shown in the circle. For BGISEQ-500, each sample was sheared and tagged with a unique barcode to prepare libraries, then equal amounts of DNA fragments from 8 samples were pooled together for DNB formation, loading, and sequencing. In total, 20 samples were sequenced in 3 lanes (F0, G0, and H0). Of them, DNA from 8 subjects (S01-S08) was utilized to perform library construction and sequencing twice; the corresponding 8 paired datasets from lane I0 (green) and lane F0 (blue) were considered library replicates. DNBs from the same 8 subjects were loaded and sequenced twice to generate 8 paired sequencing replicates (lane F0 and lane F1). Twenty datasets from HiSeq 2000 were also generated in this study. The detailed assessment and comparison analyses of metagenomic datasets between intra- and inter-platforms are shown below.

    Journal: GigaScience

    Article Title: Assessment of the cPAS-based BGISEQ-500 platform for metagenomic sequencing

    doi: 10.1093/gigascience/gix133

    Figure Lengend Snippet: Schematic model summarizing the study design and analysis strategy. Schematic diagram depicting the process of data generation, including collection of fecal samples and extraction of DNA from 20 healthy subjects, library preparation, and sequencing strategy for BGISEQ-500 and HiSeq 2000. Each circle indicates 1 independent subject, with subject ID shown in the circle. For BGISEQ-500, each sample was sheared and tagged with a unique barcode to prepare libraries, then equal amounts of DNA fragments from 8 samples were pooled together for DNB formation, loading, and sequencing. In total, 20 samples were sequenced in 3 lanes (F0, G0, and H0). Of them, DNA from 8 subjects (S01-S08) was utilized to perform library construction and sequencing twice; the corresponding 8 paired datasets from lane I0 (green) and lane F0 (blue) were considered library replicates. DNBs from the same 8 subjects were loaded and sequenced twice to generate 8 paired sequencing replicates (lane F0 and lane F1). Twenty datasets from HiSeq 2000 were also generated in this study. The detailed assessment and comparison analyses of metagenomic datasets between intra- and inter-platforms are shown below.

    Article Snippet: To further document the quantitative consistency and performance regarding GC content observed for the BGISEQ-500 and HiSeq 2000 platforms, the same 20 DNA samples were processed to construct libraries and sequenced on an Illumina HiSeq 4000 platform using the HiSeq 3000/4000SBS Kit (300 cycles) for 100 bp of paired-end reads.

    Techniques: Sequencing, Generated

    Effect of the integration procedures on TCGA LUSC datasets. Two datasets comprising of Illumina HiSeq-2000 and Affymetrix HG-U133A data were employed to evaluate the effect of the integration on two different platforms. After applying the integration procedure, DEGs were computed varying the p-value threshold. Receiver-Operating Characteristic (ROC) curves were computed using known DEGs, and the performances were assessed by means of Area Under the ROC Curve (AUC)

    Journal: BMC Bioinformatics

    Article Title: TACITuS: transcriptomic data collector, integrator, and selector on big data platform

    doi: 10.1186/s12859-019-2912-4

    Figure Lengend Snippet: Effect of the integration procedures on TCGA LUSC datasets. Two datasets comprising of Illumina HiSeq-2000 and Affymetrix HG-U133A data were employed to evaluate the effect of the integration on two different platforms. After applying the integration procedure, DEGs were computed varying the p-value threshold. Receiver-Operating Characteristic (ROC) curves were computed using known DEGs, and the performances were assessed by means of Area Under the ROC Curve (AUC)

    Article Snippet: The dataset comprises of 552 RNA-seq samples (501 cases and 51 controls) obtained using the Illumina HiSeq-2000 sequencing platform.

    Techniques:

    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.

    Journal: Molecular Systems Biology

    Article Title: Predominant contribution of cis-regulatory divergence in the evolution of mouse alternative splicing

    doi: 10.15252/msb.20145970

    Figure Lengend Snippet: Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.

    Article Snippet: Divergence in alternative splicing between C57BL/6J and SPRET/EiJ To characterize the divergence of alternative splicing between C57BL/6J and SPRET/EiJ, we derived fibroblast cell lines from the two mouse strains and sequenced three biological replicates of polyA RNAs isolated from them on an Illumina HiSeq 2000/2500 platform (Fig , Materials and Methods).

    Techniques: Isolation, Mouse Assay, Cell Culture