hiseq 2000 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Illumina Inc hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 12131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000/product/Illumina Inc
    Average 92 stars, based on 12131 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Macrogen hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000, supplied by Macrogen, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000/product/Macrogen
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    LC Sciences hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000, supplied by LC Sciences, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000/product/LC Sciences
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Agilent technologies hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000/product/Agilent technologies
    Average 92 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    BGI Inc hiseq 2000 system
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 System, supplied by BGI Inc, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 system/product/BGI Inc
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 system - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Macrogen hiseq 2000 platform
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Platform, supplied by Macrogen, used in various techniques. Bioz Stars score: 92/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 platform/product/Macrogen
    Average 92 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 platform - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    93
    GATC Biotech hiseq 2000 platform
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Platform, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 platform/product/GATC Biotech
    Average 93 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 platform - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    Agilent technologies illumina hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Illumina Hiseq 2000, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Agilent technologies
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2000 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    PerkinElmer illumina hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Illumina Hiseq 2000, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/PerkinElmer
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2000 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    94
    Illumina Inc hiseq 2000 analyzer
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Analyzer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 analyzer/product/Illumina Inc
    Average 94 stars, based on 185 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 analyzer - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    88
    Illumina Inc hiseq tm 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq Tm 2000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq tm 2000/product/Illumina Inc
    Average 88 stars, based on 98 article reviews
    Price from $9.99 to $1999.99
    hiseq tm 2000 - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    88
    Illumina Inc hiseq 2000 v4lane
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 V4lane, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 v4lane/product/Illumina Inc
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 v4lane - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    92
    Fasteris Life illumina hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Illumina Hiseq 2000, supplied by Fasteris Life, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Fasteris Life
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2000 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    91
    Theragen Etex hiseq 2000 platform
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Platform, supplied by Theragen Etex, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 platform/product/Theragen Etex
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 platform - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    92
    BGI Shenzhen hiseq 2000 platform
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Platform, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 92/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 platform/product/BGI Shenzhen
    Average 92 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 platform - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    BGI Shenzhen hiseq 2000 sequencer
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Sequencer, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 92/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 sequencer/product/BGI Shenzhen
    Average 92 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 sequencer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    89
    Illumina Inc hiseq 2000 apparatus
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Apparatus, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 apparatus/product/Illumina Inc
    Average 89 stars, based on 120 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 apparatus - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    99
    Illumina Inc hiseq 2000 machine
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Machine, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 machine/product/Illumina Inc
    Average 99 stars, based on 1245 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 machine - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.

    Journal: Molecules

    Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)

    doi: 10.3390/molecules201219758

    Figure Lengend Snippet: Frequency distribution of the contig sizes from two Gynostemma species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.

    Article Snippet: Purified RNA was used to construct a directional cDNA library using the cDNA Synthesis Kit (Illumina), and then the cDNA library was sequenced using a HiSeq 2000 (Illumina) to obtain short sequences.

    Techniques: Sequencing

    Validation of RNA-Seq data by quantitative real-time PCR (qRT-PCR).  (A)  Comparison of the expressions profile of eight DEGs determined by Illumina HiSeq™ 2000 sequencing platform and qRT-PCR at 48 h (pi) using ribosomal protein subunit S4 ( rps4 ) as housekeeping gene. Data shown are the mean of triplicates ± SD.  (B)  Correlation of data between RNA-Seq and qRT-PCR techniques.

    Journal: Frontiers in Immunology

    Article Title: Transcriptomic Profiles of Senegalese Sole Infected With Nervous Necrosis Virus Reassortants Presenting Different Degree of Virulence

    doi: 10.3389/fimmu.2018.01626

    Figure Lengend Snippet: Validation of RNA-Seq data by quantitative real-time PCR (qRT-PCR). (A) Comparison of the expressions profile of eight DEGs determined by Illumina HiSeq™ 2000 sequencing platform and qRT-PCR at 48 h (pi) using ribosomal protein subunit S4 ( rps4 ) as housekeeping gene. Data shown are the mean of triplicates ± SD. (B) Correlation of data between RNA-Seq and qRT-PCR techniques.

    Article Snippet: Therefore, samples from 48 h p.i. were selected for RNA-Seq analysis (threefold replicated), being sequenced using Illumina HiSeq™ 2000 platform.

    Techniques: RNA Sequencing Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Sequencing

    Representative terpenoid biosynthesis pathway with cognate heat maps for transcript levels of genes from S. guaranitica transcriptome data with substrates and products, coloured arrows connect substrates to their corresponding products. Green/red colour-coded heat maps represent relative transcript levels of different terpenoid genes determined by Illumina HiSeq 2000 sequencing; red, up-regulated; green, down-regulated. Transcript levels data represent by FPKM: fragments per kilobase of transcripts per million mapped fragments. MeV, multi-experiment Viewer software was used to depict transcript levels. DXS , 1-deoxy- d -xylulose-5-phosphate synthase; DXR , 1-deoxy- d -xylulose-5-phosphate reductoisomerase; MCT , 2-C-methyl- d -erythritol 4-phosphate cytidylyltransferase; ISPF , 2-C-methyl- d -erythritol 2, 4-cyclodiphos-phate synthase; HDS , (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase; HDR , 4-hydroxy-3-methylbut-2-enyl diphosphate reductases; IDI , isopentenyl-diphosphate delta isomerase; AACT , acetyl-CoA C-acetyl transferase; HMGS , hydroxyl methyl glutaryl-CoA synthase; HMGR , hydroxymethyl glutaryl-CoA reductase (NADPH); MVK , mevalonate kinase; PMK , phospho-mevalonate kinase; GPPS , geranyl diphosphate synthase; FPPS , farnesyl pyrophosphate synthase; GGPS , geranylgeranyl diphosphate synthase, type II; CINS , 1,8-cineole synthase; MYS , myrcene/ocimene synthase; LINS , (3S)-linalool synthase; NEOM , (+)-neomenthol dehydrogenase; SABI , (+)-sabinene synthase; TPS6 , (-)-germacrene d synthase; AMS , beta-amyrin synthase; FARNESOL , farnesol dehydrogenase; SEQ , squalene monooxygenase; HUMS , α-humulene/β-caryophyllene synthase; FAR , farnesyl-diphosphate farnesyltransferase; GA2 , gibberellin 2-oxidase; GA20 , gibberellin 20-oxidase; E-KS , ent-kaurene synthase; MAS , momilactone-A synthase; GA3 , gibberellin 3-beta-dioxygenase; E-KIA , ent-iso-kaurene C2-hydroxylase; E-KIH , ent-kaurenoic acid hydroxylase; E-CDS , ent-copalyl diphosphate synthase.

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.)

    doi: 10.1093/dnares/dsy028

    Figure Lengend Snippet: Representative terpenoid biosynthesis pathway with cognate heat maps for transcript levels of genes from S. guaranitica transcriptome data with substrates and products, coloured arrows connect substrates to their corresponding products. Green/red colour-coded heat maps represent relative transcript levels of different terpenoid genes determined by Illumina HiSeq 2000 sequencing; red, up-regulated; green, down-regulated. Transcript levels data represent by FPKM: fragments per kilobase of transcripts per million mapped fragments. MeV, multi-experiment Viewer software was used to depict transcript levels. DXS , 1-deoxy- d -xylulose-5-phosphate synthase; DXR , 1-deoxy- d -xylulose-5-phosphate reductoisomerase; MCT , 2-C-methyl- d -erythritol 4-phosphate cytidylyltransferase; ISPF , 2-C-methyl- d -erythritol 2, 4-cyclodiphos-phate synthase; HDS , (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase; HDR , 4-hydroxy-3-methylbut-2-enyl diphosphate reductases; IDI , isopentenyl-diphosphate delta isomerase; AACT , acetyl-CoA C-acetyl transferase; HMGS , hydroxyl methyl glutaryl-CoA synthase; HMGR , hydroxymethyl glutaryl-CoA reductase (NADPH); MVK , mevalonate kinase; PMK , phospho-mevalonate kinase; GPPS , geranyl diphosphate synthase; FPPS , farnesyl pyrophosphate synthase; GGPS , geranylgeranyl diphosphate synthase, type II; CINS , 1,8-cineole synthase; MYS , myrcene/ocimene synthase; LINS , (3S)-linalool synthase; NEOM , (+)-neomenthol dehydrogenase; SABI , (+)-sabinene synthase; TPS6 , (-)-germacrene d synthase; AMS , beta-amyrin synthase; FARNESOL , farnesol dehydrogenase; SEQ , squalene monooxygenase; HUMS , α-humulene/β-caryophyllene synthase; FAR , farnesyl-diphosphate farnesyltransferase; GA2 , gibberellin 2-oxidase; GA20 , gibberellin 20-oxidase; E-KS , ent-kaurene synthase; MAS , momilactone-A synthase; GA3 , gibberellin 3-beta-dioxygenase; E-KIA , ent-iso-kaurene C2-hydroxylase; E-KIH , ent-kaurenoic acid hydroxylase; E-CDS , ent-copalyl diphosphate synthase.

    Article Snippet: SSR discovery and analysis The Illumina HiSeq 2000 system offers the opportunity to analyse molecular markers such as SSRs that are related to terpenoid pathway genes.

    Techniques: Sequencing, Software, Affinity Magnetic Separation

    Sampling and major sources of variation . Strains CT43 and ATCC10792 grown in two medium lots #1091744 and 7220443 in water taken from building 1520 and 1610. Bacteria were cultured on four different dates and four biological replicates were grown to mid-log phase for each date, harvested and then RNA-seq data were generated using an Illumina Hiseq 2000 instrument.

    Journal: Frontiers in Microbiology

    Article Title: Replicates, Read Numbers, and Other Important Experimental Design Considerations for Microbial RNA-seq Identified Using Bacillus thuringiensis Datasets

    doi: 10.3389/fmicb.2016.00794

    Figure Lengend Snippet: Sampling and major sources of variation . Strains CT43 and ATCC10792 grown in two medium lots #1091744 and 7220443 in water taken from building 1520 and 1610. Bacteria were cultured on four different dates and four biological replicates were grown to mid-log phase for each date, harvested and then RNA-seq data were generated using an Illumina Hiseq 2000 instrument.

    Article Snippet: Samples were diluted according to manufacturer's recommendations using the Illumina dilution calculator, and sequence data was generated via two runs using an Illumina HiSeq 2000 instrument with SR50 sequencing kits (50 bp single end reads) and using phiX control DNA (Illumina, Inc., San Diego, CA, USA), as previously described (Wilson et al., ).

    Techniques: Sampling, Cell Culture, RNA Sequencing Assay, Generated

    Performance summary of mRRBS . Ninety-six samples were processed using mRRBS and sequenced with eight lanes of Illumina HiSeq 2000 using 12 barcoded adapters per lane. (a) The total number of reads for each sample is shown 84 samples with > 5 million total reads were included in the subsequent comparisons. (b) Quartile plots of summary coverage depth from these samples. The minimum and maximum values are bounded by the light blue area in (b-d), while the darker blue area represents the interquartile range. The dark blue line indicates the median. (c,d) MspI in silico digestion of the hg19 genome produced a total of 1,124,739 fragments. (c) The percentage of fragments of each fragment size that were covered by at least one read. (d) The average coverage depth for fragments of each length. Genomic MspI-digested fragments longer than 300 bp were not included in the sequence alignment target, which partly contributes to the sharp drop in coverage at 300 bp in (c,d).

    Journal: Genome Biology

    Article Title: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling

    doi: 10.1186/gb-2012-13-10-r92

    Figure Lengend Snippet: Performance summary of mRRBS . Ninety-six samples were processed using mRRBS and sequenced with eight lanes of Illumina HiSeq 2000 using 12 barcoded adapters per lane. (a) The total number of reads for each sample is shown 84 samples with > 5 million total reads were included in the subsequent comparisons. (b) Quartile plots of summary coverage depth from these samples. The minimum and maximum values are bounded by the light blue area in (b-d), while the darker blue area represents the interquartile range. The dark blue line indicates the median. (c,d) MspI in silico digestion of the hg19 genome produced a total of 1,124,739 fragments. (c) The percentage of fragments of each fragment size that were covered by at least one read. (d) The average coverage depth for fragments of each length. Genomic MspI-digested fragments longer than 300 bp were not included in the sequence alignment target, which partly contributes to the sharp drop in coverage at 300 bp in (c,d).

    Article Snippet: To evaluate the performance of the mRRBS protocol, we sequenced the 96 libraries using 8 lanes of an Illumina HiSeq 2000 sequencer with 12 libraries per lane, which produced a median of 11.3 million reads per library (Table and Figure ; Additional file ).

    Techniques: In Silico, Produced, Sequencing

    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using Illumina Hiseq 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.

    Journal: PLoS ONE

    Article Title: Systematic Analysis of Small RNAs Associated with Human Mitochondria by Deep Sequencing: Detailed Analysis of Mitochondrial Associated miRNA

    doi: 10.1371/journal.pone.0044873

    Figure Lengend Snippet: Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using Illumina Hiseq 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.

    Article Snippet: Bioinformatics Analysis of sRNA Libraries from Mitochondria All the 50 nt sequence tags generated from Illumina HiSeq 2000 went through data cleaning process which included getting rid of low quality tags.

    Techniques: Generated, Sequencing

    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.

    Journal: PLoS ONE

    Article Title: Familial episodic ataxia in lambs is potentially associated with a mutation in the fibroblast growth factor 14 (FGF14) gene

    doi: 10.1371/journal.pone.0190030

    Figure Lengend Snippet: Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.

    Article Snippet: Two lanes of paired-end reads (2 x 100 bp) were then obtained using an Illumina HiSeq 2000 machine (Illumina Inc., San Diego, CA, USA).

    Techniques: Sequencing, Mutagenesis, Variant Assay

    Chromatogram showing the location of the mutation Y: C/T in heterozygous, affected, and unrelated normal sheep. The c.46C > T variant was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.

    Journal: PLoS ONE

    Article Title: Familial episodic ataxia in lambs is potentially associated with a mutation in the fibroblast growth factor 14 (FGF14) gene

    doi: 10.1371/journal.pone.0190030

    Figure Lengend Snippet: Chromatogram showing the location of the mutation Y: C/T in heterozygous, affected, and unrelated normal sheep. The c.46C > T variant was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.

    Article Snippet: Two lanes of paired-end reads (2 x 100 bp) were then obtained using an Illumina HiSeq 2000 machine (Illumina Inc., San Diego, CA, USA).

    Techniques: Mutagenesis, Variant Assay, Sequencing