hiseq 2000 Search Results


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  • 88
    Macrogen hiseq 2000
    Hiseq 2000, supplied by Macrogen, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000/product/Macrogen
    Average 88 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 - by Bioz Stars, 2020-02
    88/100 stars
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    99
    Illumina Inc hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 12322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000/product/Illumina Inc
    Average 99 stars, based on 12322 article reviews
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    hiseq 2000 - by Bioz Stars, 2020-02
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    94
    LC Sciences hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000, supplied by LC Sciences, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hiseq 2000 - by Bioz Stars, 2020-02
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    96
    BGI Shenzhen hiseq 2000 platform
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Platform, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 96/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 90 article reviews
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    hiseq 2000 platform - by Bioz Stars, 2020-02
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    97
    PerkinElmer illumina hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Illumina Hiseq 2000, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 97/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GATC Biotech hiseq 2000 platform
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Platform, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc hiseq 2000 flowcell
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Flowcell, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc hiseq 2000 analyzer
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 Analyzer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Illumina Inc hiseq 2000 1x100
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Hiseq 2000 1x100, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 83/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    LGC Genomics GmbH illumina hiseq 2000
    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.
    Illumina Hiseq 2000, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc hiseq 2000 machine
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Machine, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Novogene hiseq 2000 machine
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Machine, supplied by Novogene, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Integragen illumina hiseq 2000
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Illumina Hiseq 2000, supplied by Integragen, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GATC Biotech hiseq 2000 sequencer
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Sequencer, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    BGI Shenzhen illumina hiseq 2000
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Illumina Hiseq 2000, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 89/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Novogene hiseq 2000 platform
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Platform, supplied by Novogene, used in various techniques. Bioz Stars score: 96/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Illumina Inc hiseq 2000 100pe
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 100pe, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    LC Sciences hiseq 2000 platform
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Platform, supplied by LC Sciences, used in various techniques. Bioz Stars score: 96/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc hiseq 2000 2500
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 70 article reviews
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    89
    Eurofins illumina hiseq 2000
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Illumina Hiseq 2000, supplied by Eurofins, used in various techniques. Bioz Stars score: 89/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 23 article reviews
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    88
    Macrogen hiseq 2000 sequencer
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Sequencer, supplied by Macrogen, used in various techniques. Bioz Stars score: 88/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 19 article reviews
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    88
    BGI Shenzhen hiseq 2000 sequencer
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Sequencer, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 88/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 41 article reviews
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    97
    Vazyme Biotech Co illumina hiseq 2000
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Illumina Hiseq 2000, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 24 article reviews
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    87
    BGI Shenzhen hiseq 2000 machine
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Machine, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Illumina Inc illumin hiseq 2000
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Illumin Hiseq 2000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Biomarker Technologies Co illumina hiseq 2000
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Illumina Hiseq 2000, supplied by Biomarker Technologies Co, used in various techniques. Bioz Stars score: 89/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 49 article reviews
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    88
    SciGenom hiseq 2000 sequencer
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Sequencer, supplied by SciGenom, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Fasteris Life illumina hiseq 2000
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Illumina Hiseq 2000, supplied by Fasteris Life, used in various techniques. Bioz Stars score: 89/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc hiseq 2000 apparatus
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Apparatus, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Illumina Inc hiseq 2000 guidelines
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Guidelines, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 82/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Illumina Inc hiseq 2000 ilumina
    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina <t>HiSeq</t> 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
    Hiseq 2000 Ilumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Frequency distribution of the contig sizes from two  Gynostemma  species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.

    Journal: Molecules

    Article Title: Characterization of Global Transcriptome Using Illumina Paired-End Sequencing and Development of EST-SSR Markers in Two Species of Gynostemma (Cucurbitaceae)

    doi: 10.3390/molecules201219758

    Figure Lengend Snippet: Frequency distribution of the contig sizes from two Gynostemma species. The frequency distribution of contig sizes resulting from Illumina HiSeq™ 2000 sequencing, as assembled using Trinity.

    Article Snippet: Purified RNA was used to construct a directional cDNA library using the cDNA Synthesis Kit (Illumina), and then the cDNA library was sequenced using a HiSeq 2000 (Illumina) to obtain short sequences.

    Techniques: Sequencing

    Sampling and major sources of variation . Strains CT43 and ATCC10792 grown in two medium lots #1091744 and 7220443 in water taken from building 1520 and 1610. Bacteria were cultured on four different dates and four biological replicates were grown to mid-log phase for each date, harvested and then RNA-seq data were generated using an Illumina Hiseq 2000 instrument.

    Journal: Frontiers in Microbiology

    Article Title: Replicates, Read Numbers, and Other Important Experimental Design Considerations for Microbial RNA-seq Identified Using Bacillus thuringiensis Datasets

    doi: 10.3389/fmicb.2016.00794

    Figure Lengend Snippet: Sampling and major sources of variation . Strains CT43 and ATCC10792 grown in two medium lots #1091744 and 7220443 in water taken from building 1520 and 1610. Bacteria were cultured on four different dates and four biological replicates were grown to mid-log phase for each date, harvested and then RNA-seq data were generated using an Illumina Hiseq 2000 instrument.

    Article Snippet: Samples were diluted according to manufacturer's recommendations using the Illumina dilution calculator, and sequence data was generated via two runs using an Illumina HiSeq 2000 instrument with SR50 sequencing kits (50 bp single end reads) and using phiX control DNA (Illumina, Inc., San Diego, CA, USA), as previously described (Wilson et al., ).

    Techniques: Sampling, Cell Culture, RNA Sequencing Assay, Generated

    Representative terpenoid biosynthesis pathway with cognate heat maps for transcript levels of genes from S. guaranitica transcriptome data with substrates and products, coloured arrows connect substrates to their corresponding products. Green/red colour-coded heat maps represent relative transcript levels of different terpenoid genes determined by Illumina HiSeq 2000 sequencing; red, up-regulated; green, down-regulated. Transcript levels data represent by FPKM: fragments per kilobase of transcripts per million mapped fragments. MeV, multi-experiment Viewer software was used to depict transcript levels. DXS , 1-deoxy- d -xylulose-5-phosphate synthase; DXR , 1-deoxy- d -xylulose-5-phosphate reductoisomerase; MCT , 2-C-methyl- d -erythritol 4-phosphate cytidylyltransferase; ISPF , 2-C-methyl- d -erythritol 2, 4-cyclodiphos-phate synthase; HDS , (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase; HDR , 4-hydroxy-3-methylbut-2-enyl diphosphate reductases; IDI , isopentenyl-diphosphate delta isomerase; AACT , acetyl-CoA C-acetyl transferase; HMGS , hydroxyl methyl glutaryl-CoA synthase; HMGR , hydroxymethyl glutaryl-CoA reductase (NADPH); MVK , mevalonate kinase; PMK , phospho-mevalonate kinase; GPPS , geranyl diphosphate synthase; FPPS , farnesyl pyrophosphate synthase; GGPS , geranylgeranyl diphosphate synthase, type II; CINS , 1,8-cineole synthase; MYS , myrcene/ocimene synthase; LINS , (3S)-linalool synthase; NEOM , (+)-neomenthol dehydrogenase; SABI , (+)-sabinene synthase; TPS6 , (-)-germacrene d synthase; AMS , beta-amyrin synthase; FARNESOL , farnesol dehydrogenase; SEQ , squalene monooxygenase; HUMS , α-humulene/β-caryophyllene synthase; FAR , farnesyl-diphosphate farnesyltransferase; GA2 , gibberellin 2-oxidase; GA20 , gibberellin 20-oxidase; E-KS , ent-kaurene synthase; MAS , momilactone-A synthase; GA3 , gibberellin 3-beta-dioxygenase; E-KIA , ent-iso-kaurene C2-hydroxylase; E-KIH , ent-kaurenoic acid hydroxylase; E-CDS , ent-copalyl diphosphate synthase.

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.)

    doi: 10.1093/dnares/dsy028

    Figure Lengend Snippet: Representative terpenoid biosynthesis pathway with cognate heat maps for transcript levels of genes from S. guaranitica transcriptome data with substrates and products, coloured arrows connect substrates to their corresponding products. Green/red colour-coded heat maps represent relative transcript levels of different terpenoid genes determined by Illumina HiSeq 2000 sequencing; red, up-regulated; green, down-regulated. Transcript levels data represent by FPKM: fragments per kilobase of transcripts per million mapped fragments. MeV, multi-experiment Viewer software was used to depict transcript levels. DXS , 1-deoxy- d -xylulose-5-phosphate synthase; DXR , 1-deoxy- d -xylulose-5-phosphate reductoisomerase; MCT , 2-C-methyl- d -erythritol 4-phosphate cytidylyltransferase; ISPF , 2-C-methyl- d -erythritol 2, 4-cyclodiphos-phate synthase; HDS , (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase; HDR , 4-hydroxy-3-methylbut-2-enyl diphosphate reductases; IDI , isopentenyl-diphosphate delta isomerase; AACT , acetyl-CoA C-acetyl transferase; HMGS , hydroxyl methyl glutaryl-CoA synthase; HMGR , hydroxymethyl glutaryl-CoA reductase (NADPH); MVK , mevalonate kinase; PMK , phospho-mevalonate kinase; GPPS , geranyl diphosphate synthase; FPPS , farnesyl pyrophosphate synthase; GGPS , geranylgeranyl diphosphate synthase, type II; CINS , 1,8-cineole synthase; MYS , myrcene/ocimene synthase; LINS , (3S)-linalool synthase; NEOM , (+)-neomenthol dehydrogenase; SABI , (+)-sabinene synthase; TPS6 , (-)-germacrene d synthase; AMS , beta-amyrin synthase; FARNESOL , farnesol dehydrogenase; SEQ , squalene monooxygenase; HUMS , α-humulene/β-caryophyllene synthase; FAR , farnesyl-diphosphate farnesyltransferase; GA2 , gibberellin 2-oxidase; GA20 , gibberellin 20-oxidase; E-KS , ent-kaurene synthase; MAS , momilactone-A synthase; GA3 , gibberellin 3-beta-dioxygenase; E-KIA , ent-iso-kaurene C2-hydroxylase; E-KIH , ent-kaurenoic acid hydroxylase; E-CDS , ent-copalyl diphosphate synthase.

    Article Snippet: Illumina sequencing and the de novo assembly of the S. guaranitica leaf transcriptome In the past few years, the Illumina sequencing platform has become a powerful method for analysing and discovering the genomes of non-model plants., In this context, to generate transcriptome sequences, complementary DNA (cDNA) libraries were prepared from leaf tissues of S. guaranitica , and cDNA was then sequenced using PE reads sequencing using an Illumina HiSeq 2000 platform.

    Techniques: Sequencing, Software, Affinity Magnetic Separation

    Depth and Breadth of On-Target Coverage of the 100 Lung Cancer Samples. Shown for each tumor specimen is the percentage of targeted bases covered at given coverage depth (1x, 20x, 50x, 100x) and sequenced under different lane settings in the HiSeq 2000 instrument (2, 4, and 8 DNA libraries per lane, Lib/Ln).

    Journal: PLoS ONE

    Article Title: Combined Targeted DNA Sequencing in Non-Small Cell Lung Cancer (NSCLC) Using UNCseq and NGScopy, and RNA Sequencing Using UNCqeR for the Detection of Genetic Aberrations in NSCLC

    doi: 10.1371/journal.pone.0129280

    Figure Lengend Snippet: Depth and Breadth of On-Target Coverage of the 100 Lung Cancer Samples. Shown for each tumor specimen is the percentage of targeted bases covered at given coverage depth (1x, 20x, 50x, 100x) and sequenced under different lane settings in the HiSeq 2000 instrument (2, 4, and 8 DNA libraries per lane, Lib/Ln).

    Article Snippet: Clusters were then generated using TruSeq SR Cluster Kit v.2 and were loaded into the HiSeq 2000 sequencer (Illumina Inc., San Diego, CA).

    Techniques:

    Distribution of frequency (absolute numbers) over the length. The constructed unigenes from de-novo assembly of Cephalotaxus hainanensis short reads were generated by Illumina Hiseq 2000 sequencing. The total number of assembled unigenes is 39,416 unigene.

    Journal: PLoS ONE

    Article Title: De Novo Characterization of a Cephalotaxus hainanensis Transcriptome and Genes Related to Paclitaxel Biosynthesis

    doi: 10.1371/journal.pone.0106900

    Figure Lengend Snippet: Distribution of frequency (absolute numbers) over the length. The constructed unigenes from de-novo assembly of Cephalotaxus hainanensis short reads were generated by Illumina Hiseq 2000 sequencing. The total number of assembled unigenes is 39,416 unigene.

    Article Snippet: Subsequently, the Illumina Paired End Sample Prep kit (Illumina, USA) was used to construct a RNA-seq library which then was sequenced by Illumina HiSeq 2000 (Illumina, San Diego, CA).

    Techniques: Construct, Generated, Sequencing

    Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.

    Journal: PLoS ONE

    Article Title: Familial episodic ataxia in lambs is potentially associated with a mutation in the fibroblast growth factor 14 (FGF14) gene

    doi: 10.1371/journal.pone.0190030

    Figure Lengend Snippet: Protein sequence alignment of fibroblast growth factor 14. Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.

    Article Snippet: Two lanes of paired-end reads (2 x 100 bp) were then obtained using an Illumina HiSeq 2000 machine (Illumina Inc., San Diego, CA, USA).

    Techniques: Sequencing, Mutagenesis, Variant Assay

    Chromatogram showing the location of the mutation Y: C/T in heterozygous, affected, and unrelated normal sheep. The c.46C > T variant was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.

    Journal: PLoS ONE

    Article Title: Familial episodic ataxia in lambs is potentially associated with a mutation in the fibroblast growth factor 14 (FGF14) gene

    doi: 10.1371/journal.pone.0190030

    Figure Lengend Snippet: Chromatogram showing the location of the mutation Y: C/T in heterozygous, affected, and unrelated normal sheep. The c.46C > T variant was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.

    Article Snippet: Two lanes of paired-end reads (2 x 100 bp) were then obtained using an Illumina HiSeq 2000 machine (Illumina Inc., San Diego, CA, USA).

    Techniques: Mutagenesis, Variant Assay, Sequencing