Journal: Nucleic Acids Research
Article Title: Single molecule identification of homology-dependent interactions between long ssRNA and dsDNA
Figure Lengend Snippet: The effect of long ssRNA on dsDNA extension at low force (e.g. Figure 2B ) is dependent on concentration, homology, and length. Box-and-whisker plots (see Materials and Methods) show the slopes of the low force–extension curves (Figure 2B ) of the indicated samples. ( A ) DNA:RNA molar ratio was varied from 1:1 to 1:10 5 . Statistical analysis (asterisks) compared 1:1 versus all other ratios. ( B ) The effects of non-homologous ssRNA controls. Fluc is a 1766-nt control transcript provided in the T7 Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA. Y-RNA is the total RNA of S. cerevisiae strain. For 6KM13, bacteriophage M13mp18 circular ssDNA ∼7200 nt was amplified as the template for in vitro transcription. The resulting ssRNA is 6001 nt and presents 44% identity with the genome of λ dsDNA (region 22 893–31 255). 6L and 6H are ∼6k nt species homologous to adjacent regions of λ dsDNA (Figure 1B ). DNA:RNA molar ratio = 1:1000 in all samples; statistical analysis was performed for the ratio of λ dsDNA alone versus all other ratios. ( C ) The effects of various lengths of ssRNA (described in Figure 1B ), DNA:RNA molar ratio = 1:1000. (a, b, c) n = 10, * P
Article Snippet: Fluc is a 1766-nt control transcript provided in the T7 Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA.
Techniques: Concentration Assay, Whisker Assay, Amplification, In Vitro