Journal: Protein Expression and Purification

Article Title: Bacterial expression of human kynurenine 3-monooxygenase: Solubility, activity, purification

doi: 10.1016/j.pep.2013.11.015

Figure Lengend Snippet: Purified trKMO. (A) Chromatogram produced during NiNTA affinity purification of truncate KMO. (B) SDS-PAGE protein gel stained with simplyblue showing HisTrap purification of truncated human KMO protein, (a) Cell-free extract prior to affinity chromatography, (b) flowthrough proteins which did not bind to the column, (c) first column wash, (d) second column wash, (e) third column wash, (f) pure truncated human KMO protein (44 kDa) from the fractions under the chromatogram. (C) Cartoon illustrating the trKMO construct. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The soluble supernatant fraction was purified on a 5 ml HisTrap ff crude column (GE Healthcare, 17-5286-01) using the AKTA protein purification system (GE Healthcare).

Techniques: Purification, Produced, Affinity Purification, SDS Page, Staining, Affinity Chromatography, Construct

Journal: The Journal of Biological Chemistry

Article Title: Biochemical Characterization and Substrate Specificity of Autophagin-2 from the Parasite Trypanosoma cruzi *

doi: 10.1074/jbc.M115.687764

Figure Lengend Snippet: A , expression and purification of recombinant proteins. Proteins were expressed in E. coli BL21(DE3) strain. All proteins were expressed in fusion with the His 6 tag. Expressed recombinant proteins were purified using HisTrap FF crude columns (GE Healthcare).

Article Snippet: All proteins were expressed as fusion protein with a His6 tag and were allowed to bind to HisTrap FF crude columns (GE Healthcare) and eluted with buffer containing 250 m m imidazole.

Techniques: Expressing, Purification, Recombinant