Journal: Nature Communications
Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
Figure Lengend Snippet: Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.
Article Snippet: The monoclonal anti-MBP (Ambmart) or anti-HIS (CW Biotech) antibody was used for western blotting analysis (The information of antibodies in and Uncropped versions of blots in ).
Techniques: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay