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  • 99
    New England Biolabs hinf i
    Identification of the Abe GH5-1 gene by Southern blotting. Genomic <t>DNA</t> isolated from the five Aphelenchoides besseyi isolates were digested with <t>Hinf</t> I , and it was hybridized with an AbeFm-GH5-specific DNA probe.
    Hinf I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 464 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher hinf i
    Nuclear HRGβ2 negatively regulates telomere length in breast cancer cells A. Left. Representative immunoblot showing shRNA-mediated knockdown of HRGβ 2 in MDA-MB-231 cells. Right. Flow-FISH analysis of cells in the G 0 /G 1 phase was performed using a telomere-specific probe as described in the“Materials and methods” section. (−) and (+) indicate Flow-FISH analysis in the absence or presence, respectively, of the telomeric probe. Bivariate cytograms indicate the telomere length in each sub-compartment of the cell cycle. The histograms show the telomere length within the G 0 /G 1 cellsubpopulation, where the cells have only one copy of the genome. Telomere length is expressed in relative fluorescence units (mean ± SD) of four independent experiments. B. MCF-7 cells were transiently transfected with GFP-HRGβ 2 or GFP-HRGβ 2 ΔNLS, and telomere length was analyzed by Flow-FISH as described above (n = 3). C. MCF-7 cells were stably transduced with either pBABE retroviral vector alone or pBABE containing cDNA for HRGβ 2 ΔNLS (a structural mutant of HRGβ 2 that lacks the putative NLS), HRGβ 2 ΔEGF (a structural mutant of HRGβ 2 that cannot bind HER3 or HER4 receptors), or for full-length HRGβ 2 as specified. Telomere length was assessed by Southern blot analysis of Hinf I/ Rsa I–digestedgenomic <t>DNA</t> as described in the “Materials and methods” section. Molecular weight (MW) markers (black bars, in kb) and mean terminal restriction fragment lengths (TRFL; red bars) are indicated.
    Hinf I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    TaKaRa hinfi
    tsFT20 at 38°C showed an extended G-tail before telomeric <t>DNA</t> elongated. A. G-tail signals. DNA was prepared from FM3A or tsFT20 cells that were cultured at 33°C or 38°C for 1 or 9 weeks, treated or untreated with Exo I, and <t>HinfI</t>
    Hinfi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega hinf i
    Agarose gel with different groups of restriction. Line 1 and 11, MW ladder (100 bp); line 2, ARDRA group 1 ( L. plantarum ); line 3, ARDRA group 2 ( P. acidilactici ); line 4, ARDRA group 3 ( W. paramesenteroides ); line 5, ARDRA group 4 ( L. salivarius ); line 6, ARDRA group 5 ( L. ruminis ); line 7, ARDRA group 6 ( L. curvatus ); line 8, ARDRA group 7 ( L. farciminis ); line 9, ARDRA group 8 ( L. mucosae ); line 10, ARDRA group 9 ( E. hirae ). Restriction fragments obtained with each enzyme: (a) Hae <t>III,</t> (b) Msp I, (c) Hinf I.
    Hinf I, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher hinf i restriction endonuclease
    Patterns generated by mitochondrial DNA-RFLP with <t>Hinf</t> I restriction endonuclease of indigenous Saccharomyces cerevisiae and commercial strains. Lanes: 1, 1 Kb plus DNA Ladder; 2, commercial yeast S. cerevisiae A, profile P6; 3, commercial S. cerevisiae B, profile P7; 4, commercial S. cerevisiae C, profile P8; 5, commercial S. cerevisiae D, profile P5; 6, commercial S. bayanus F, profile P8; 7, commercial S. cerevisiae E, profile P5; 8, strain LMA-V68, profile P1; 9, strain LMA-V65, profile P5; 10, strain LMA-V80, profile P2.
    Hinf I Restriction Endonuclease, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher hinf i enzyme
    Gel image of PCR products from 17 nematode populations restricted with <t>Hinf</t> I enzyme. Lanes showing two fragments of 1.0 and 0.7 kb were related M. javanica whereas lanes with three fragments of 1.0, 0.4 and 0.3 kb were related to M. incognita . The products were resolved on 1.5% agarose gel and stained with ethidium bromide. M lanes were loaded with 1 kb ladder.
    Hinf I Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega restriction enzyme hinf i
    Gel image of PCR products from 17 nematode populations restricted with <t>Hinf</t> I enzyme. Lanes showing two fragments of 1.0 and 0.7 kb were related M. javanica whereas lanes with three fragments of 1.0, 0.4 and 0.3 kb were related to M. incognita . The products were resolved on 1.5% agarose gel and stained with ethidium bromide. M lanes were loaded with 1 kb ladder.
    Restriction Enzyme Hinf I, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher hinf 1 enzyme
    Gel image of PCR products from 17 nematode populations restricted with <t>Hinf</t> I enzyme. Lanes showing two fragments of 1.0 and 0.7 kb were related M. javanica whereas lanes with three fragments of 1.0, 0.4 and 0.3 kb were related to M. incognita . The products were resolved on 1.5% agarose gel and stained with ethidium bromide. M lanes were loaded with 1 kb ladder.
    Hinf 1 Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs hinf i restriction endonuclease
    Gel image of PCR products from 17 nematode populations restricted with <t>Hinf</t> I enzyme. Lanes showing two fragments of 1.0 and 0.7 kb were related M. javanica whereas lanes with three fragments of 1.0, 0.4 and 0.3 kb were related to M. incognita . The products were resolved on 1.5% agarose gel and stained with ethidium bromide. M lanes were loaded with 1 kb ladder.
    Hinf I Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sangon Biotech hinf i digested fingerprint pattern
    Gel image of PCR products from 17 nematode populations restricted with <t>Hinf</t> I enzyme. Lanes showing two fragments of 1.0 and 0.7 kb were related M. javanica whereas lanes with three fragments of 1.0, 0.4 and 0.3 kb were related to M. incognita . The products were resolved on 1.5% agarose gel and stained with ethidium bromide. M lanes were loaded with 1 kb ladder.
    Hinf I Digested Fingerprint Pattern, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bangalore Genei hinf i restriction enzymes
    Combined dendrogram of genetic relatedness generated by NTSYSpc ver. 2.0 from the PCR-restriction fragment length polymorphism banding pattern analysis of the PCR amplified internal transcribed spacer, small subunit rDNA and β-tubulin gene products with the <t>Msp</t> I, Alu I, Nde II, Hae III, and <t>Hinf</t> I restriction enzymes. The neighbour-joining algorithm and Jaccard's similarity coefficient were used to generate the trees.
    Hinf I Restriction Enzymes, supplied by Bangalore Genei, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Boehringer Mannheim hinf i
    Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with <t>Hinf</t> I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 <t>DNA</t> cut with Bgl I and Hinf I restriction enzymes.
    Hinf I, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SibEnzyme hinf i
    Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with <t>Hinf</t> I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 <t>DNA</t> cut with Bgl I and Hinf I restriction enzymes.
    Hinf I, supplied by SibEnzyme, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore hinfi
    Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with <t>Hinf</t> I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 <t>DNA</t> cut with Bgl I and Hinf I restriction enzymes.
    Hinfi, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher hinfi
    Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with <t>Hinf</t> I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 <t>DNA</t> cut with Bgl I and Hinf I restriction enzymes.
    Hinfi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher 407 bp hinf i
    Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with <t>Hinf</t> I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 <t>DNA</t> cut with Bgl I and Hinf I restriction enzymes.
    407 Bp Hinf I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega dna hinf i marker
    Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with <t>Hinf</t> I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 <t>DNA</t> cut with Bgl I and Hinf I restriction enzymes.
    Dna Hinf I Marker, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    GE Healthcare restriction endonuclease hinf i
    Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with <t>Hinf</t> I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 <t>DNA</t> cut with Bgl I and Hinf I restriction enzymes.
    Restriction Endonuclease Hinf I, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Identification of the Abe GH5-1 gene by Southern blotting. Genomic DNA isolated from the five Aphelenchoides besseyi isolates were digested with Hinf I , and it was hybridized with an AbeFm-GH5-specific DNA probe.

    Journal: PLoS ONE

    Article Title: Glycoside Hydrolase (GH) 45 and 5 Candidate Cellulases in Aphelenchoides besseyi Isolated from Bird’s-Nest Fern

    doi: 10.1371/journal.pone.0158663

    Figure Lengend Snippet: Identification of the Abe GH5-1 gene by Southern blotting. Genomic DNA isolated from the five Aphelenchoides besseyi isolates were digested with Hinf I , and it was hybridized with an AbeFm-GH5-specific DNA probe.

    Article Snippet: Southern blotting and in situ hybridization of the Abe GH5-1 gene Approximately 10 to 20 μg of genomic DNA from the 5 different A . besseyi isolates was digested with Hinf I (NEB, California, USA) overnight at 37°C.

    Techniques: Southern Blot, Isolation

    Nuclear HRGβ2 negatively regulates telomere length in breast cancer cells A. Left. Representative immunoblot showing shRNA-mediated knockdown of HRGβ 2 in MDA-MB-231 cells. Right. Flow-FISH analysis of cells in the G 0 /G 1 phase was performed using a telomere-specific probe as described in the“Materials and methods” section. (−) and (+) indicate Flow-FISH analysis in the absence or presence, respectively, of the telomeric probe. Bivariate cytograms indicate the telomere length in each sub-compartment of the cell cycle. The histograms show the telomere length within the G 0 /G 1 cellsubpopulation, where the cells have only one copy of the genome. Telomere length is expressed in relative fluorescence units (mean ± SD) of four independent experiments. B. MCF-7 cells were transiently transfected with GFP-HRGβ 2 or GFP-HRGβ 2 ΔNLS, and telomere length was analyzed by Flow-FISH as described above (n = 3). C. MCF-7 cells were stably transduced with either pBABE retroviral vector alone or pBABE containing cDNA for HRGβ 2 ΔNLS (a structural mutant of HRGβ 2 that lacks the putative NLS), HRGβ 2 ΔEGF (a structural mutant of HRGβ 2 that cannot bind HER3 or HER4 receptors), or for full-length HRGβ 2 as specified. Telomere length was assessed by Southern blot analysis of Hinf I/ Rsa I–digestedgenomic DNA as described in the “Materials and methods” section. Molecular weight (MW) markers (black bars, in kb) and mean terminal restriction fragment lengths (TRFL; red bars) are indicated.

    Journal: Oncotarget

    Article Title: Heregulin, a new interactor of the telosome/shelterin complex in human telomeres

    doi:

    Figure Lengend Snippet: Nuclear HRGβ2 negatively regulates telomere length in breast cancer cells A. Left. Representative immunoblot showing shRNA-mediated knockdown of HRGβ 2 in MDA-MB-231 cells. Right. Flow-FISH analysis of cells in the G 0 /G 1 phase was performed using a telomere-specific probe as described in the“Materials and methods” section. (−) and (+) indicate Flow-FISH analysis in the absence or presence, respectively, of the telomeric probe. Bivariate cytograms indicate the telomere length in each sub-compartment of the cell cycle. The histograms show the telomere length within the G 0 /G 1 cellsubpopulation, where the cells have only one copy of the genome. Telomere length is expressed in relative fluorescence units (mean ± SD) of four independent experiments. B. MCF-7 cells were transiently transfected with GFP-HRGβ 2 or GFP-HRGβ 2 ΔNLS, and telomere length was analyzed by Flow-FISH as described above (n = 3). C. MCF-7 cells were stably transduced with either pBABE retroviral vector alone or pBABE containing cDNA for HRGβ 2 ΔNLS (a structural mutant of HRGβ 2 that lacks the putative NLS), HRGβ 2 ΔEGF (a structural mutant of HRGβ 2 that cannot bind HER3 or HER4 receptors), or for full-length HRGβ 2 as specified. Telomere length was assessed by Southern blot analysis of Hinf I/ Rsa I–digestedgenomic DNA as described in the “Materials and methods” section. Molecular weight (MW) markers (black bars, in kb) and mean terminal restriction fragment lengths (TRFL; red bars) are indicated.

    Article Snippet: Protein-free DNA was cleaved with Hinf I and Rsa Irestriction enzymes (Gibco/BRL), extracted once with phenol/chloroform/isoamyl alcohol, ethanol precipitated, and resuspendedin Tris-EDTA to generate the TRF.

    Techniques: shRNA, Multiple Displacement Amplification, Flow Cytometry, Fluorescence In Situ Hybridization, Fluorescence, Transfection, Stable Transfection, Transduction, Plasmid Preparation, Mutagenesis, Southern Blot, Molecular Weight

    tsFT20 at 38°C showed an extended G-tail before telomeric DNA elongated. A. G-tail signals. DNA was prepared from FM3A or tsFT20 cells that were cultured at 33°C or 38°C for 1 or 9 weeks, treated or untreated with Exo I, and HinfI

    Journal: Molecular and Cellular Biology

    Article Title: Alterations of DNA and Chromatin Structures at Telomeres and Genetic Instability in Mouse Cells Defective in DNA Polymerase ? †

    doi: 10.1128/MCB.25.24.11073-11088.2005

    Figure Lengend Snippet: tsFT20 at 38°C showed an extended G-tail before telomeric DNA elongated. A. G-tail signals. DNA was prepared from FM3A or tsFT20 cells that were cultured at 33°C or 38°C for 1 or 9 weeks, treated or untreated with Exo I, and HinfI

    Article Snippet: Genomic DNA was digested with HinfI (0.3 U/μl; TaKaRa) overnight at 37°C.

    Techniques: Cell Culture

    PCR-RFLP analysis of mtDNA cox1 for molecular identification of anisakid larvae. Each PCR product was digested with Hinf I and analyzed on agarose gel. Lanes 1-3, A. pegreffii (each from S. schlegeli , C. myriaster , and H. otakii , respectively); Lanes 4-6, A. simplex s. str. (each from G. macrocephalus , O. masou masou , and O. keta , respectively); Lane M, 100 bp ladder.

    Journal: The Korean Journal of Parasitology

    Article Title: Molecular Analysis of Anisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea

    doi: 10.3347/kjp.2014.52.4.383

    Figure Lengend Snippet: PCR-RFLP analysis of mtDNA cox1 for molecular identification of anisakid larvae. Each PCR product was digested with Hinf I and analyzed on agarose gel. Lanes 1-3, A. pegreffii (each from S. schlegeli , C. myriaster , and H. otakii , respectively); Lanes 4-6, A. simplex s. str. (each from G. macrocephalus , O. masou masou , and O. keta , respectively); Lane M, 100 bp ladder.

    Article Snippet: RFLP analysis Each PCR product was digested with 10 units of each restriction endonuclease, Hinf I (Takara) or Hha I (Takara), respectively, in a volume of 20 µl at 37℃ for 3 hr [ ].

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    PCR-RFLP analysis of rDNA ITS for molecular identification of anisakid larvae. Each PCR product was digested with Hinf I or Hha I and analyzed on agarose gel. (A) Hinf I digestion. (B) Hha I digestion. Lanes 1-2, A. simplex s. str. (each from G. macrocephalus and O. keta , respectively); Lanes 3-9, A. pegreffii (each from L. japonicus , S. thompson , C. myriaster , H. otakii , E. japonica , G. macrocephalus , and P. olivaceus , respectively); Lane M, 100 bp ladder.

    Journal: The Korean Journal of Parasitology

    Article Title: Molecular Analysis of Anisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea

    doi: 10.3347/kjp.2014.52.4.383

    Figure Lengend Snippet: PCR-RFLP analysis of rDNA ITS for molecular identification of anisakid larvae. Each PCR product was digested with Hinf I or Hha I and analyzed on agarose gel. (A) Hinf I digestion. (B) Hha I digestion. Lanes 1-2, A. simplex s. str. (each from G. macrocephalus and O. keta , respectively); Lanes 3-9, A. pegreffii (each from L. japonicus , S. thompson , C. myriaster , H. otakii , E. japonica , G. macrocephalus , and P. olivaceus , respectively); Lane M, 100 bp ladder.

    Article Snippet: RFLP analysis Each PCR product was digested with 10 units of each restriction endonuclease, Hinf I (Takara) or Hha I (Takara), respectively, in a volume of 20 µl at 37℃ for 3 hr [ ].

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Agarose gel with different groups of restriction. Line 1 and 11, MW ladder (100 bp); line 2, ARDRA group 1 ( L. plantarum ); line 3, ARDRA group 2 ( P. acidilactici ); line 4, ARDRA group 3 ( W. paramesenteroides ); line 5, ARDRA group 4 ( L. salivarius ); line 6, ARDRA group 5 ( L. ruminis ); line 7, ARDRA group 6 ( L. curvatus ); line 8, ARDRA group 7 ( L. farciminis ); line 9, ARDRA group 8 ( L. mucosae ); line 10, ARDRA group 9 ( E. hirae ). Restriction fragments obtained with each enzyme: (a) Hae III, (b) Msp I, (c) Hinf I.

    Journal: Veterinary Medicine International

    Article Title: Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use

    doi: 10.4061/2010/274987

    Figure Lengend Snippet: Agarose gel with different groups of restriction. Line 1 and 11, MW ladder (100 bp); line 2, ARDRA group 1 ( L. plantarum ); line 3, ARDRA group 2 ( P. acidilactici ); line 4, ARDRA group 3 ( W. paramesenteroides ); line 5, ARDRA group 4 ( L. salivarius ); line 6, ARDRA group 5 ( L. ruminis ); line 7, ARDRA group 6 ( L. curvatus ); line 8, ARDRA group 7 ( L. farciminis ); line 9, ARDRA group 8 ( L. mucosae ); line 10, ARDRA group 9 ( E. hirae ). Restriction fragments obtained with each enzyme: (a) Hae III, (b) Msp I, (c) Hinf I.

    Article Snippet: Three restriction enzymes were used: Hae III, Msp I and Hinf I (Promega).

    Techniques: Agarose Gel Electrophoresis

    Patterns generated by mitochondrial DNA-RFLP with Hinf I restriction endonuclease of indigenous Saccharomyces cerevisiae and commercial strains. Lanes: 1, 1 Kb plus DNA Ladder; 2, commercial yeast S. cerevisiae A, profile P6; 3, commercial S. cerevisiae B, profile P7; 4, commercial S. cerevisiae C, profile P8; 5, commercial S. cerevisiae D, profile P5; 6, commercial S. bayanus F, profile P8; 7, commercial S. cerevisiae E, profile P5; 8, strain LMA-V68, profile P1; 9, strain LMA-V65, profile P5; 10, strain LMA-V80, profile P2.

    Journal: Brazilian Journal of Microbiology

    Article Title: Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley

    doi:

    Figure Lengend Snippet: Patterns generated by mitochondrial DNA-RFLP with Hinf I restriction endonuclease of indigenous Saccharomyces cerevisiae and commercial strains. Lanes: 1, 1 Kb plus DNA Ladder; 2, commercial yeast S. cerevisiae A, profile P6; 3, commercial S. cerevisiae B, profile P7; 4, commercial S. cerevisiae C, profile P8; 5, commercial S. cerevisiae D, profile P5; 6, commercial S. bayanus F, profile P8; 7, commercial S. cerevisiae E, profile P5; 8, strain LMA-V68, profile P1; 9, strain LMA-V65, profile P5; 10, strain LMA-V80, profile P2.

    Article Snippet: The mtDNA of isolates was purified as described by , and digested with Hinf I restriction endonuclease (Invitrogen, USA).

    Techniques: Generated

    Patterns generated by mitochondrial DNA-RFLP with Hinf I restriction endonuclease of indigenous Saccharomyces cerevisiae isolates from fermented grape must ( Vitis vinifera L.). Lanes: 1, 1 Kb plus DNA Ladder; 2, profile P5 (strain LMA-V65); 3, profile P5 (commercial S. cerevisiae E); 4, profile P2 (strain LMA-V80); 5, profile P3 (strain LMA-V148); 6, profile P1 (strain LMA-V68); 7, profile P1 (strain LMA-V132); 8, profile P4 (strain LMA-V152).

    Journal: Brazilian Journal of Microbiology

    Article Title: Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley

    doi:

    Figure Lengend Snippet: Patterns generated by mitochondrial DNA-RFLP with Hinf I restriction endonuclease of indigenous Saccharomyces cerevisiae isolates from fermented grape must ( Vitis vinifera L.). Lanes: 1, 1 Kb plus DNA Ladder; 2, profile P5 (strain LMA-V65); 3, profile P5 (commercial S. cerevisiae E); 4, profile P2 (strain LMA-V80); 5, profile P3 (strain LMA-V148); 6, profile P1 (strain LMA-V68); 7, profile P1 (strain LMA-V132); 8, profile P4 (strain LMA-V152).

    Article Snippet: The mtDNA of isolates was purified as described by , and digested with Hinf I restriction endonuclease (Invitrogen, USA).

    Techniques: Generated

    Number of isolates of Saccharomyces cerevisiae yeasts for each pattern of mitochondrial DNA-RFLP with Hinf I restriction endonuclease obtained from different grape varieties studied in the São Francisco Valley region, northeastern Brazil.

    Journal: Brazilian Journal of Microbiology

    Article Title: Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley

    doi:

    Figure Lengend Snippet: Number of isolates of Saccharomyces cerevisiae yeasts for each pattern of mitochondrial DNA-RFLP with Hinf I restriction endonuclease obtained from different grape varieties studied in the São Francisco Valley region, northeastern Brazil.

    Article Snippet: The mtDNA of isolates was purified as described by , and digested with Hinf I restriction endonuclease (Invitrogen, USA).

    Techniques:

    Gel image of PCR products from 17 nematode populations restricted with Hinf I enzyme. Lanes showing two fragments of 1.0 and 0.7 kb were related M. javanica whereas lanes with three fragments of 1.0, 0.4 and 0.3 kb were related to M. incognita . The products were resolved on 1.5% agarose gel and stained with ethidium bromide. M lanes were loaded with 1 kb ladder.

    Journal: The Plant Pathology Journal

    Article Title: Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

    doi: 10.5423/PPJ.OA.11.2017.0253

    Figure Lengend Snippet: Gel image of PCR products from 17 nematode populations restricted with Hinf I enzyme. Lanes showing two fragments of 1.0 and 0.7 kb were related M. javanica whereas lanes with three fragments of 1.0, 0.4 and 0.3 kb were related to M. incognita . The products were resolved on 1.5% agarose gel and stained with ethidium bromide. M lanes were loaded with 1 kb ladder.

    Article Snippet: A 20 μl restriction reaction was assembled using 10 μl of amplified product, 7 μl of nucleases free water, 2 μl of 10× buffer R [10 mM Tris-HCl (pH 8.5), 10 mM MgCl2 , 100 mM KCl, 0.1 mg/ml BSA] and 1 μl (10 units) of Hinf I enzyme (Fermentas, USA).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Combined dendrogram of genetic relatedness generated by NTSYSpc ver. 2.0 from the PCR-restriction fragment length polymorphism banding pattern analysis of the PCR amplified internal transcribed spacer, small subunit rDNA and β-tubulin gene products with the Msp I, Alu I, Nde II, Hae III, and Hinf I restriction enzymes. The neighbour-joining algorithm and Jaccard's similarity coefficient were used to generate the trees.

    Journal: Mycobiology

    Article Title: Multi-loci Molecular Characterisation of Endophytic Fungi Isolated from Five Medicinal Plants of Meghalaya, India

    doi: 10.4489/MYCO.2011.39.2.071

    Figure Lengend Snippet: Combined dendrogram of genetic relatedness generated by NTSYSpc ver. 2.0 from the PCR-restriction fragment length polymorphism banding pattern analysis of the PCR amplified internal transcribed spacer, small subunit rDNA and β-tubulin gene products with the Msp I, Alu I, Nde II, Hae III, and Hinf I restriction enzymes. The neighbour-joining algorithm and Jaccard's similarity coefficient were used to generate the trees.

    Article Snippet: PCR-RFLP RFLP patterns of the PCR amplified ITS, small subunit (SSU) rDNA and β-tubulin gene products were generated with the Msp I, Alu I, Nde II, Hae III and Hinf I restriction enzymes (Bangalore Genei).

    Techniques: Generated, Polymerase Chain Reaction, Amplification

    Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with Hinf I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 DNA cut with Bgl I and Hinf I restriction enzymes.

    Journal: Clinical and Experimental Immunology

    Article Title: Differential expression of cytokine transcripts in human epithelial ovarian carcinoma by solid tumour specimens, peritoneal exudate cells containing tumour, tumour-infiltrating lymphocyte (TIL)-derived T cell lines and established tumour cell lines

    doi: 10.1046/j.1365-2249.1998.00576.x

    Figure Lengend Snippet: Restriction enzyme digestion analysis of IL-2 polymerase chain reaction (PCR) products. IL-2 primer-specific PCR products (100 μl reaction for each digestion) from a solid tumour specimen and a CD4 + cell line were digested with Hinf I or Xha I. The restriction digests were displayed on a 5% polyacrylamide/0.1 × TBE gel. Molecular markers (M) were pBR 328 DNA cut with Bgl I and Hinf I restriction enzymes.

    Article Snippet: Molecular weight markers consisted of a commercially prepared 100-bp ladder (G ibco -BRL) or pBR328 plasmid DNA cut with restriction enzymes Bgl I and Hinf I (Boehringer Mannheim).

    Techniques: Polymerase Chain Reaction