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  • 99
    New England Biolabs restriction sites hindiii
    Restriction Sites Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction sites hindiii/product/New England Biolabs
    Average 99 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    restriction sites hindiii - by Bioz Stars, 2020-07
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    99
    Thermo Fisher hindiii
    The NDR is modified along S phase. (A) , ARS603 is represented by a red rectangle and flanking genes, YFL034W and YFL033C, by blue ones. Vertical bars indicate sites for digestion with restriction enzymes PvuII and <t>HindIII.</t> Green rectangle represents the end-terminal probe. The nucleosomal profile from the G1 sample is presented below. (B) , Naked DNA and chromatin from wild-type cells collected at 20, 40 60 and 80 min after α-factor arrest and release into YPD were digested with increasing amounts of MNase (indicated with triangles) and subsequently digested with PvuII and HindIII. Samples were electrophoresed, transferred onto a membrane and hybridized. A ladder of polynucleosomes is observed with a prominent region of hypersensitivity to MNase corresponding to the NDR (indicated in brackets in A , B ). The black arrow and asterisk point to the changes in chromatin organization observed in the NDR at later time points.
    Hindiii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hindiii/product/Thermo Fisher
    Average 99 stars, based on 9246 article reviews
    Price from $9.99 to $1999.99
    hindiii - by Bioz Stars, 2020-07
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    99
    Thermo Fisher fermentas hindiii
    Control digestions of recombinant plasmid DNA using <t>HindIII</t> (Fermentas) enzyme. (A) pIL253:PptcB:MOG35-55, (B) pIL253:PptcB:MBP85-97, (C) pIL253:PptcB:PLP139-151. Expected DNA fragments after restriction analysis for correct constructs: 3897 bp, 845 bp (marked by red arrows), 1kb DNA ladder (M) 1kb DNA Ladder (Fermentas) used as DNA size reference marker.
    Fermentas Hindiii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fermentas hindiii/product/Thermo Fisher
    Average 99 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    fermentas hindiii - by Bioz Stars, 2020-07
    99/100 stars
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    Image Search Results


    The NDR is modified along S phase. (A) , ARS603 is represented by a red rectangle and flanking genes, YFL034W and YFL033C, by blue ones. Vertical bars indicate sites for digestion with restriction enzymes PvuII and HindIII. Green rectangle represents the end-terminal probe. The nucleosomal profile from the G1 sample is presented below. (B) , Naked DNA and chromatin from wild-type cells collected at 20, 40 60 and 80 min after α-factor arrest and release into YPD were digested with increasing amounts of MNase (indicated with triangles) and subsequently digested with PvuII and HindIII. Samples were electrophoresed, transferred onto a membrane and hybridized. A ladder of polynucleosomes is observed with a prominent region of hypersensitivity to MNase corresponding to the NDR (indicated in brackets in A , B ). The black arrow and asterisk point to the changes in chromatin organization observed in the NDR at later time points.

    Journal: BMC Genomics

    Article Title: Different nucleosomal architectures at early and late replicating origins in Saccharomyces cerevisiae

    doi: 10.1186/1471-2164-15-791

    Figure Lengend Snippet: The NDR is modified along S phase. (A) , ARS603 is represented by a red rectangle and flanking genes, YFL034W and YFL033C, by blue ones. Vertical bars indicate sites for digestion with restriction enzymes PvuII and HindIII. Green rectangle represents the end-terminal probe. The nucleosomal profile from the G1 sample is presented below. (B) , Naked DNA and chromatin from wild-type cells collected at 20, 40 60 and 80 min after α-factor arrest and release into YPD were digested with increasing amounts of MNase (indicated with triangles) and subsequently digested with PvuII and HindIII. Samples were electrophoresed, transferred onto a membrane and hybridized. A ladder of polynucleosomes is observed with a prominent region of hypersensitivity to MNase corresponding to the NDR (indicated in brackets in A , B ). The black arrow and asterisk point to the changes in chromatin organization observed in the NDR at later time points.

    Article Snippet: For each sample, 2.5 μg of DNA were digested with HindIII and PvuII enzymes (Fermentas), separated by electrophoresis in a 1.5% agarose gel, blotted and hybridized to an specific end-terminal probe.

    Techniques: Modification

    The pAVOIAF{#1–#2–#3–#4} vector. ( A ) Vector map of pAVOIAF{#1–#2–#3–#4}. The vector is based on the pUC57-Kan vector, from which only the kanamycin resistance cassette and the origin of replication remain. The four-slot cloning site together with the 3’ and 5’ piggyBac terminal repeats is located between the AatII and PciI sites. The light gray band on the inside indicates the transgene. ( B ) Scheme of the four-slot cloning site. Each slot consists of an 18 bp spacer that translates into the amino acids Phe-Arg-Glu-Asp-Asp-Tyr and thus was termed FREDDY spacer. The slots can be accessed individually by unique restriction enzyme site pairs (XmaI/SpeI for #1, HindIII/XbaI for #2, XhoI/NheI for #3 and AflII/AvrII for #4). They are embedded into five PmeI restriction enzyme sites that allow a simple one-enzyme control digestion to determine the size of the sequences that were inserted into the slots. For convenience, the downstream restriction enzyme sites for each slot (SpeI for #1, XbaI for #2, NheI for #3 and AvrII #4) result in identical sticky ends, facilitating cloning procedures that cannot utilize the suggested restriction enzyme site pairs. Extends of the genetic elements are not to scale. ORF, open-reading frame.

    Journal: eLife

    Article Title: A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines

    doi: 10.7554/eLife.31677

    Figure Lengend Snippet: The pAVOIAF{#1–#2–#3–#4} vector. ( A ) Vector map of pAVOIAF{#1–#2–#3–#4}. The vector is based on the pUC57-Kan vector, from which only the kanamycin resistance cassette and the origin of replication remain. The four-slot cloning site together with the 3’ and 5’ piggyBac terminal repeats is located between the AatII and PciI sites. The light gray band on the inside indicates the transgene. ( B ) Scheme of the four-slot cloning site. Each slot consists of an 18 bp spacer that translates into the amino acids Phe-Arg-Glu-Asp-Asp-Tyr and thus was termed FREDDY spacer. The slots can be accessed individually by unique restriction enzyme site pairs (XmaI/SpeI for #1, HindIII/XbaI for #2, XhoI/NheI for #3 and AflII/AvrII for #4). They are embedded into five PmeI restriction enzyme sites that allow a simple one-enzyme control digestion to determine the size of the sequences that were inserted into the slots. For convenience, the downstream restriction enzyme sites for each slot (SpeI for #1, XbaI for #2, NheI for #3 and AvrII #4) result in identical sticky ends, facilitating cloning procedures that cannot utilize the suggested restriction enzyme site pairs. Extends of the genetic elements are not to scale. ORF, open-reading frame.

    Article Snippet: Molecular biology: the pGS[#P’#O(LA)-mEmerald] and pAGOC{#P’#O(LA)-mEmerald} vectors A hybrid sequence, consisting of (i) a HindIII site, (ii) the modular fluorescent protein expression cassette as described above and (iii) a XbaI site, was de novo synthetized and inserted into the unique SfiI site of pMK-RQ (Thermo Fisher Scientific).

    Techniques: Plasmid Preparation, Clone Assay

    Strategy to construct a self-excisable HSV-1(F) BAC clone. (A) Lines 1 and 2, schematic diagrams of E. coli plasmid pYEbac102 and pYEbac102Cre, respectively, contained in GS1783; line 3, schematic diagrams of recombinant virus YK312 (ΔBAC). (B) Schematic diagram of the genome structures of wild-type HSV-1(F) and relevant domains of the recombinant viruses. Line 1, sequence arrangement of the HSV-1(F) genome. Line 2, location of the EcoRV-HindIII fragment used as a labeled probe in panel C. Line 3, an enlarged portion of the EcoRI J and D fragments of HSV-1(F). Lines 4 and 6, double-headed arrow represents the fragment region. Lines 5 and 7, summary of DNA fragments from recombinant viruses with or without the BAC sequence and the poly(A) signals. (C) Southern blot analysis of EcoRI-digested DNA isolated from YK312 (ΔBAC)- or YK304 (BAC)-infected cells using the EcoRV-HindIII fragment of pRB442 as a probe. The letters on the right refer to the designations of the DNA fragments generated by restriction endonuclease EcoRI cleavage.

    Journal: Journal of Virology

    Article Title: Roles of the Phosphorylation of Herpes Simplex Virus 1 UL51 at a Specific Site in Viral Replication and Pathogenicity

    doi: 10.1128/JVI.01035-18

    Figure Lengend Snippet: Strategy to construct a self-excisable HSV-1(F) BAC clone. (A) Lines 1 and 2, schematic diagrams of E. coli plasmid pYEbac102 and pYEbac102Cre, respectively, contained in GS1783; line 3, schematic diagrams of recombinant virus YK312 (ΔBAC). (B) Schematic diagram of the genome structures of wild-type HSV-1(F) and relevant domains of the recombinant viruses. Line 1, sequence arrangement of the HSV-1(F) genome. Line 2, location of the EcoRV-HindIII fragment used as a labeled probe in panel C. Line 3, an enlarged portion of the EcoRI J and D fragments of HSV-1(F). Lines 4 and 6, double-headed arrow represents the fragment region. Lines 5 and 7, summary of DNA fragments from recombinant viruses with or without the BAC sequence and the poly(A) signals. (C) Southern blot analysis of EcoRI-digested DNA isolated from YK312 (ΔBAC)- or YK304 (BAC)-infected cells using the EcoRV-HindIII fragment of pRB442 as a probe. The letters on the right refer to the designations of the DNA fragments generated by restriction endonuclease EcoRI cleavage.

    Article Snippet: This was then cloned into the EcoRI site of pcDNA3.1(+) (Thermo Fisher Scientific). pcDNA-FEM-KanS was constructed by PCR amplification of the domain containing the FEM tag, I-SceI site, and kanamycin resistance gene from pBS-FEM-KanS and cloning it into the EcoRI and HindIII sites of pcDNA3.1/myc-His(−)-A (Thermo Fisher Scientific). pcDNA-FEM was constructed by digesting pcDNA-FEM-KanS with NheI and self-ligating to remove the I-SceI site and kanamycin resistance gene. pcDNA-FEM-UL51 was constructed by amplifying the entire UL51 open reading frame (ORF) from pYEbac102 by PCR and cloning it into pcDNA-FEM in frame with FEM. pBS1007, used to generate the recombinant virus YK639 (ΔUL51-repair), was described previously ( ). pRB442, used to generate the probe for Southern blotting, contains a 6.3-kbp KpnI-HindIII fragment of HSV-1(F), which encompasses the left end of the UL region, including the region between 0.0415 and 0.084 map units, as described previously ( ).

    Techniques: Construct, BAC Assay, Plasmid Preparation, Recombinant, Sequencing, Labeling, Southern Blot, Isolation, Infection, Generated

    Control digestions of recombinant plasmid DNA using HindIII (Fermentas) enzyme. (A) pIL253:PptcB:MOG35-55, (B) pIL253:PptcB:MBP85-97, (C) pIL253:PptcB:PLP139-151. Expected DNA fragments after restriction analysis for correct constructs: 3897 bp, 845 bp (marked by red arrows), 1kb DNA ladder (M) 1kb DNA Ladder (Fermentas) used as DNA size reference marker.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Oral Administration of Lactococcus lactis Expressing Synthetic Genes of Myelin Antigens in Decreasing Experimental Autoimmune Encephalomyelitis in Rats

    doi: 10.12659/MSM.892764

    Figure Lengend Snippet: Control digestions of recombinant plasmid DNA using HindIII (Fermentas) enzyme. (A) pIL253:PptcB:MOG35-55, (B) pIL253:PptcB:MBP85-97, (C) pIL253:PptcB:PLP139-151. Expected DNA fragments after restriction analysis for correct constructs: 3897 bp, 845 bp (marked by red arrows), 1kb DNA ladder (M) 1kb DNA Ladder (Fermentas) used as DNA size reference marker.

    Article Snippet: From confirmed proper recombinant cells, plasmid DNA was isolated using the Plasmid Mini Kit (A & A Biotechnology) and subjected to restriction analysis with HindIII enzyme (Fermentas).

    Techniques: Recombinant, Plasmid Preparation, Construct, Marker

    Confirmation of genomic composition of 3 independent recombinant VV-ΔTk viruses. ( A ) An ethidium bromide stained DNA gel of genomic HindIII restriction digests of viral DNA isolated from parental VV (Wyeth Strain), 3 clones of VV-ΔTk- yfp-gpt and 3 clones of VV-ΔTk'. Arrows indicate the Tk insertion site (VV-Wyeth), and the unique bands that result from insertion of the yfp-gpt cassette (VV-ΔTk- yfp-gpt ). ( B ) Southern hybridization of the DNA gel in A identifying the yfp insert present in the genome of the VV-ΔTk- yfp-gpt clones, but not in parental VV-Wyeth or the VV-ΔTk' clones. ( C ) DNAStar sequence alignment at the yfp-gpt insertion site of DNA isolated from the 3 VV-ΔTk' clones post Cre passage.

    Journal: PLoS ONE

    Article Title: A Selectable and Excisable Marker System for the Rapid Creation of Recombinant Poxviruses

    doi: 10.1371/journal.pone.0024643

    Figure Lengend Snippet: Confirmation of genomic composition of 3 independent recombinant VV-ΔTk viruses. ( A ) An ethidium bromide stained DNA gel of genomic HindIII restriction digests of viral DNA isolated from parental VV (Wyeth Strain), 3 clones of VV-ΔTk- yfp-gpt and 3 clones of VV-ΔTk'. Arrows indicate the Tk insertion site (VV-Wyeth), and the unique bands that result from insertion of the yfp-gpt cassette (VV-ΔTk- yfp-gpt ). ( B ) Southern hybridization of the DNA gel in A identifying the yfp insert present in the genome of the VV-ΔTk- yfp-gpt clones, but not in parental VV-Wyeth or the VV-ΔTk' clones. ( C ) DNAStar sequence alignment at the yfp-gpt insertion site of DNA isolated from the 3 VV-ΔTk' clones post Cre passage.

    Article Snippet: Briefly, 7 µg of DNA was digested overnight with HindIII restriction enzyme (Invitrogen) at 37°C.

    Techniques: Recombinant, Staining, Isolation, Clone Assay, Hybridization, Sequencing