hilic Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher acclaim hilic 10
    HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim <t>HILIC-10,</t> 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.
    Acclaim Hilic 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acclaim hilic 10/product/Thermo Fisher
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    acclaim hilic 10 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    88
    Millipore hilic
    HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim <t>HILIC-10,</t> 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.
    Hilic, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic/product/Millipore
    Average 88 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    hilic - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    92
    Merck & Co hilic column
    A , Venn diagrams of the number of Meth-R sites identified by <t>HILIC,</t> IEF, and SCX individually and combined ( left ) or unique in HILIC or 7E6 mAb or found by both methods ( right ) in <t>Jurkat</t> cells and primary human CD4+ lymphocytes combined. B , Venn diagram showing the number of Meth-R sites found only in this study, the Meth-R sites annotated in UniProtKB database and those in common. C , Venn diagram of the number of arginine methylated proteins identified in this study, those previously reported in the UniProtKB database and those in common. Circles are approximately scaled relative to number of sites.
    Hilic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/Merck & Co
    Average 92 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    91
    Waters Corporation hilic column
    A , Venn diagrams of the number of Meth-R sites identified by <t>HILIC,</t> IEF, and SCX individually and combined ( left ) or unique in HILIC or 7E6 mAb or found by both methods ( right ) in <t>Jurkat</t> cells and primary human CD4+ lymphocytes combined. B , Venn diagram showing the number of Meth-R sites found only in this study, the Meth-R sites annotated in UniProtKB database and those in common. C , Venn diagram of the number of arginine methylated proteins identified in this study, those previously reported in the UniProtKB database and those in common. Circles are approximately scaled relative to number of sites.
    Hilic Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/Waters Corporation
    Average 91 stars, based on 412 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    93
    Bonna-Agela Technologies venusil hilic
    A , Venn diagrams of the number of Meth-R sites identified by <t>HILIC,</t> IEF, and SCX individually and combined ( left ) or unique in HILIC or 7E6 mAb or found by both methods ( right ) in <t>Jurkat</t> cells and primary human CD4+ lymphocytes combined. B , Venn diagram showing the number of Meth-R sites found only in this study, the Meth-R sites annotated in UniProtKB database and those in common. C , Venn diagram of the number of arginine methylated proteins identified in this study, those previously reported in the UniProtKB database and those in common. Circles are approximately scaled relative to number of sites.
    Venusil Hilic, supplied by Bonna-Agela Technologies, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/venusil hilic/product/Bonna-Agela Technologies
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    venusil hilic - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    MACHEREY NAGEL chromabondr hilic
    A , Venn diagrams of the number of Meth-R sites identified by <t>HILIC,</t> IEF, and SCX individually and combined ( left ) or unique in HILIC or 7E6 mAb or found by both methods ( right ) in <t>Jurkat</t> cells and primary human CD4+ lymphocytes combined. B , Venn diagram showing the number of Meth-R sites found only in this study, the Meth-R sites annotated in UniProtKB database and those in common. C , Venn diagram of the number of arginine methylated proteins identified in this study, those previously reported in the UniProtKB database and those in common. Circles are approximately scaled relative to number of sites.
    Chromabondr Hilic, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chromabondr hilic/product/MACHEREY NAGEL
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    chromabondr hilic - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    85
    Nacalai cosmosil hilic
    A , Venn diagrams of the number of Meth-R sites identified by <t>HILIC,</t> IEF, and SCX individually and combined ( left ) or unique in HILIC or 7E6 mAb or found by both methods ( right ) in <t>Jurkat</t> cells and primary human CD4+ lymphocytes combined. B , Venn diagram showing the number of Meth-R sites found only in this study, the Meth-R sites annotated in UniProtKB database and those in common. C , Venn diagram of the number of arginine methylated proteins identified in this study, those previously reported in the UniProtKB database and those in common. Circles are approximately scaled relative to number of sites.
    Cosmosil Hilic, supplied by Nacalai, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cosmosil hilic/product/Nacalai
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cosmosil hilic - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    93
    PolyLC INC hilic beads
    The Venn diagram analysis of intact N-glycopeptides (A) and N-glycosylation sites (B) enriched by <t>HILIC</t> and MLA methods. (C) The comparison of the percentage of <t>sialylated</t> N-glycopeptides enriched by HILIC and MLA respectively.
    Hilic Beads, supplied by PolyLC INC, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic beads/product/PolyLC INC
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    hilic beads - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    91
    Phenomenex hilic column
    Extracted ion chromatogram (XIC) of acylPG in corn roots obtained from ( a ) <t>HILIC</t> and ( b ) <t>C30-RPLC,</t> ( c ) HILIC-MS of total acylPG. Corresponding HRMS/MS spectrum of m/z 1029.76 obtained from ( d ) HILIC and ( e , f ) C30-RPLC. Structures of acylPG are inserted in the MS/MS spectra.
    Hilic Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/Phenomenex
    Average 91 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    86
    Phenomenex hilic kinetex
    Extracted ion chromatogram (XIC) of acylPG in corn roots obtained from ( a ) <t>HILIC</t> and ( b ) <t>C30-RPLC,</t> ( c ) HILIC-MS of total acylPG. Corresponding HRMS/MS spectrum of m/z 1029.76 obtained from ( d ) HILIC and ( e , f ) C30-RPLC. Structures of acylPG are inserted in the MS/MS spectra.
    Hilic Kinetex, supplied by Phenomenex, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic kinetex/product/Phenomenex
    Average 86 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    hilic kinetex - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    84
    PolyLC INC hilic toptip
    Extracted ion chromatogram (XIC) of acylPG in corn roots obtained from ( a ) <t>HILIC</t> and ( b ) <t>C30-RPLC,</t> ( c ) HILIC-MS of total acylPG. Corresponding HRMS/MS spectrum of m/z 1029.76 obtained from ( d ) HILIC and ( e , f ) C30-RPLC. Structures of acylPG are inserted in the MS/MS spectra.
    Hilic Toptip, supplied by PolyLC INC, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic toptip/product/PolyLC INC
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hilic toptip - by Bioz Stars, 2020-05
    84/100 stars
      Buy from Supplier

    88
    Merck KGaA zic hilic
    Experimental design for in-depth mapping of the N-glycoproteome in the mouse brain Mouse brain proteins were digested with seven sets of proteases. The efficiencies of the proteases were compared while <t>ZIC-HILIC</t> was used as the enrichment technique (black arrow). Then, four enrichment techniques were evaluated on tryptic peptides (black arrow). Finally, the influence of fractionation before (with SCX) or after (with bRP) enrichment with ZIC-HILIC was assessed. For all of the above experiments, N-glycans were detached with PNGase F before online RPLC-MS/MS detection.
    Zic Hilic, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic hilic/product/Merck KGaA
    Average 88 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    zic hilic - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    92
    The Nest Group zic hilic
    Experimental design for in-depth mapping of the N-glycoproteome in the mouse brain Mouse brain proteins were digested with seven sets of proteases. The efficiencies of the proteases were compared while <t>ZIC-HILIC</t> was used as the enrichment technique (black arrow). Then, four enrichment techniques were evaluated on tryptic peptides (black arrow). Finally, the influence of fractionation before (with SCX) or after (with bRP) enrichment with ZIC-HILIC was assessed. For all of the above experiments, N-glycans were detached with PNGase F before online RPLC-MS/MS detection.
    Zic Hilic, supplied by The Nest Group, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic hilic/product/The Nest Group
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    zic hilic - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    91
    The Nest Group hilic column
    Experimental design for in-depth mapping of the N-glycoproteome in the mouse brain Mouse brain proteins were digested with seven sets of proteases. The efficiencies of the proteases were compared while <t>ZIC-HILIC</t> was used as the enrichment technique (black arrow). Then, four enrichment techniques were evaluated on tryptic peptides (black arrow). Finally, the influence of fractionation before (with SCX) or after (with bRP) enrichment with ZIC-HILIC was assessed. For all of the above experiments, N-glycans were detached with PNGase F before online RPLC-MS/MS detection.
    Hilic Column, supplied by The Nest Group, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/The Nest Group
    Average 91 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    91
    Tosoh Corporation hilic column
    <t>HILIC</t> HPLC chromatogram of <t>cPOS</t> (Top) and cPOS-t (Bottom) at 235 nm. 6,7-dihydromonotropein ( a ), deacetylasperulsidic acid ( b ) and monotropein ( c ).
    Hilic Column, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 91/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/Tosoh Corporation
    Average 91 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    93
    Merck & Co zic hilic
    <t>HILIC</t> HPLC chromatogram of <t>cPOS</t> (Top) and cPOS-t (Bottom) at 235 nm. 6,7-dihydromonotropein ( a ), deacetylasperulsidic acid ( b ) and monotropein ( c ).
    Zic Hilic, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic hilic/product/Merck & Co
    Average 93 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    zic hilic - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    91
    Waters Corporation atlantis hilic
    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and <t>SFC</t> Waters <t>HILIC</t> columns.
    Atlantis Hilic, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atlantis hilic/product/Waters Corporation
    Average 91 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    atlantis hilic - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    93
    Thermo Fisher hilic column
    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and <t>SFC</t> Waters <t>HILIC</t> columns.
    Hilic Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/Thermo Fisher
    Average 93 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    Agilent technologies hilic columns
    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and <t>SFC</t> Waters <t>HILIC</t> columns.
    Hilic Columns, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic columns/product/Agilent technologies
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    hilic columns - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    91
    Waters Corporation 186006963 hilic qc
    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and <t>SFC</t> Waters <t>HILIC</t> columns.
    186006963 Hilic Qc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/186006963 hilic qc/product/Waters Corporation
    Average 91 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    186006963 hilic qc - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    93
    Waters Corporation amide hilic column
    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and <t>SFC</t> Waters <t>HILIC</t> columns.
    Amide Hilic Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amide hilic column/product/Waters Corporation
    Average 93 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    amide hilic column - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    94
    Waters Corporation atlantis hilic column
    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and <t>SFC</t> Waters <t>HILIC</t> columns.
    Atlantis Hilic Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atlantis hilic column/product/Waters Corporation
    Average 94 stars, based on 294 article reviews
    Price from $9.99 to $1999.99
    atlantis hilic column - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    90
    Waters Corporation hilic beh amide
    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and <t>SFC</t> Waters <t>HILIC</t> columns.
    Hilic Beh Amide, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic beh amide/product/Waters Corporation
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hilic beh amide - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    92
    The Nest Group ispe hilic cartridges
    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and <t>SFC</t> Waters <t>HILIC</t> columns.
    Ispe Hilic Cartridges, supplied by The Nest Group, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ispe hilic cartridges/product/The Nest Group
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    ispe hilic cartridges - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim HILIC-10, 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Chemical, computational and functional insights into the chemical stability of the Hedgehog pathway inhibitor GANT61

    doi: 10.1080/14756366.2017.1419221

    Figure Lengend Snippet: HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim HILIC-10, 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.

    Article Snippet: The column used was the Acclaim HILIC-10, 3 µm (150 × 4.6 mm I.D.), purchased from Thermo Scientific (Waltham, MA).

    Techniques: High Performance Liquid Chromatography, Hydrophilic Interaction Liquid Chromatography, Flow Cytometry, Mass Spectrometry

    A , Venn diagrams of the number of Meth-R sites identified by HILIC, IEF, and SCX individually and combined ( left ) or unique in HILIC or 7E6 mAb or found by both methods ( right ) in Jurkat cells and primary human CD4+ lymphocytes combined. B , Venn diagram showing the number of Meth-R sites found only in this study, the Meth-R sites annotated in UniProtKB database and those in common. C , Venn diagram of the number of arginine methylated proteins identified in this study, those previously reported in the UniProtKB database and those in common. Circles are approximately scaled relative to number of sites.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: A Method for Large-scale Identification of Protein Arginine Methylation *

    doi: 10.1074/mcp.M112.020743

    Figure Lengend Snippet: A , Venn diagrams of the number of Meth-R sites identified by HILIC, IEF, and SCX individually and combined ( left ) or unique in HILIC or 7E6 mAb or found by both methods ( right ) in Jurkat cells and primary human CD4+ lymphocytes combined. B , Venn diagram showing the number of Meth-R sites found only in this study, the Meth-R sites annotated in UniProtKB database and those in common. C , Venn diagram of the number of arginine methylated proteins identified in this study, those previously reported in the UniProtKB database and those in common. Circles are approximately scaled relative to number of sites.

    Article Snippet: Trypsin digests of Methyl-SILAC Jurkat or primary CD4+ lymphocytes were loaded onto a HILIC column in 90% acetonitrile.

    Techniques: Hydrophilic Interaction Liquid Chromatography, Electrofocusing, Methylation

    A , Schematic representation of our workflow. Cells were labeled with light or heavy methionine, lysed and combined in a 1:1 protein ratio. Proteins were digested with trypsin or, in some experiments, with chymotrypsin. Arginine methylated peptides (depicted as red lines ) were enriched through the use of SCX, IEF (in an OFFGEL apparatus) or HILIC and analyzed by mass spectrometry. MS spectra for putative arginine methylated peptides were manually verified for the presence of a 1:1 methyl-SILAC pair. An example methyl-SILAC pair is shown. For each separation method, the total number of Meth-R sites identified as well as the novel ones is indicated; B , Meth-R-containing tryptic peptides have distinct physico-chemical properties. The Table indicates maximum, minimum and median values for isoelectric points (pI) and GRAVY scores of Meth-R peptides identified with the indicated enrichment methods. The number of unique peptides (n) from each method used to calculate values is shown. C , 3D scatter plot of 33000 non-methylated tryptic peptides ( gray circles ) identified from pH3–11 IPG strip and 128 arginine methylated tryptic peptides ( yellow-red circles ) identified from anti-DMA-IP, HILIC, SCX and IEF. Three different views of the same plot are shown. The peptides are plotted according to pI, GRAVY score and charge state at pH 2.7, representing the different selection criteria exploited by the enrichment methods. Yellow represents a low pI and red a high pI.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: A Method for Large-scale Identification of Protein Arginine Methylation *

    doi: 10.1074/mcp.M112.020743

    Figure Lengend Snippet: A , Schematic representation of our workflow. Cells were labeled with light or heavy methionine, lysed and combined in a 1:1 protein ratio. Proteins were digested with trypsin or, in some experiments, with chymotrypsin. Arginine methylated peptides (depicted as red lines ) were enriched through the use of SCX, IEF (in an OFFGEL apparatus) or HILIC and analyzed by mass spectrometry. MS spectra for putative arginine methylated peptides were manually verified for the presence of a 1:1 methyl-SILAC pair. An example methyl-SILAC pair is shown. For each separation method, the total number of Meth-R sites identified as well as the novel ones is indicated; B , Meth-R-containing tryptic peptides have distinct physico-chemical properties. The Table indicates maximum, minimum and median values for isoelectric points (pI) and GRAVY scores of Meth-R peptides identified with the indicated enrichment methods. The number of unique peptides (n) from each method used to calculate values is shown. C , 3D scatter plot of 33000 non-methylated tryptic peptides ( gray circles ) identified from pH3–11 IPG strip and 128 arginine methylated tryptic peptides ( yellow-red circles ) identified from anti-DMA-IP, HILIC, SCX and IEF. Three different views of the same plot are shown. The peptides are plotted according to pI, GRAVY score and charge state at pH 2.7, representing the different selection criteria exploited by the enrichment methods. Yellow represents a low pI and red a high pI.

    Article Snippet: Trypsin digests of Methyl-SILAC Jurkat or primary CD4+ lymphocytes were loaded onto a HILIC column in 90% acetonitrile.

    Techniques: Labeling, Methylation, Electrofocusing, Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry, Stripping Membranes, Selection

    The Venn diagram analysis of intact N-glycopeptides (A) and N-glycosylation sites (B) enriched by HILIC and MLA methods. (C) The comparison of the percentage of sialylated N-glycopeptides enriched by HILIC and MLA respectively.

    Journal: The Analyst

    Article Title: Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD)

    doi: 10.1039/c8an00216a

    Figure Lengend Snippet: The Venn diagram analysis of intact N-glycopeptides (A) and N-glycosylation sites (B) enriched by HILIC and MLA methods. (C) The comparison of the percentage of sialylated N-glycopeptides enriched by HILIC and MLA respectively.

    Article Snippet: This is probably because sialic acid increases the hydrophilic interactions between the N-glycopeptides and HILIC beads, resulting in preferential enrichment of sialylated N-glycopeptides.

    Techniques: Hydrophilic Interaction Liquid Chromatography

    Extracted ion chromatogram (XIC) of acylPG in corn roots obtained from ( a ) HILIC and ( b ) C30-RPLC, ( c ) HILIC-MS of total acylPG. Corresponding HRMS/MS spectrum of m/z 1029.76 obtained from ( d ) HILIC and ( e , f ) C30-RPLC. Structures of acylPG are inserted in the MS/MS spectra.

    Journal: Scientific Reports

    Article Title: Targeting Modified Lipids during Routine Lipidomics Analysis using HILIC and C30 Reverse Phase Liquid Chromatography coupled to Mass Spectrometry

    doi: 10.1038/s41598-019-41556-9

    Figure Lengend Snippet: Extracted ion chromatogram (XIC) of acylPG in corn roots obtained from ( a ) HILIC and ( b ) C30-RPLC, ( c ) HILIC-MS of total acylPG. Corresponding HRMS/MS spectrum of m/z 1029.76 obtained from ( d ) HILIC and ( e , f ) C30-RPLC. Structures of acylPG are inserted in the MS/MS spectra.

    Article Snippet: Analysis of the lipid mixture using HILIC or C30-RPLC The HILIC column (Luna 3 µm, 100 × 2 mm I.D., particle size: 3 µm, pore diameter: 200 Å) was purchased from Phenomenex (Torrance, CA, USA).

    Techniques: Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry

    UHPLC-MS chromatogram in negative ion mode of the standard lipid mixture obtained after ( a ) HILIC and ( b ) C30-RPLC. ( c ) HILIC-MS spectrum of PI eluted at 22.54 min, ( d ) C30-RPLC-MS spectrum showing PI, PG and DMPE eluted at 19.85 min. ( e , f ) MS/MS spectra of PI 18:0/20:4 from HILIC and C30RPLC, respectively.

    Journal: Scientific Reports

    Article Title: Targeting Modified Lipids during Routine Lipidomics Analysis using HILIC and C30 Reverse Phase Liquid Chromatography coupled to Mass Spectrometry

    doi: 10.1038/s41598-019-41556-9

    Figure Lengend Snippet: UHPLC-MS chromatogram in negative ion mode of the standard lipid mixture obtained after ( a ) HILIC and ( b ) C30-RPLC. ( c ) HILIC-MS spectrum of PI eluted at 22.54 min, ( d ) C30-RPLC-MS spectrum showing PI, PG and DMPE eluted at 19.85 min. ( e , f ) MS/MS spectra of PI 18:0/20:4 from HILIC and C30RPLC, respectively.

    Article Snippet: Analysis of the lipid mixture using HILIC or C30-RPLC The HILIC column (Luna 3 µm, 100 × 2 mm I.D., particle size: 3 µm, pore diameter: 200 Å) was purchased from Phenomenex (Torrance, CA, USA).

    Techniques: Mass Spectrometry, Hydrophilic Interaction Liquid Chromatography

    Experimental design for in-depth mapping of the N-glycoproteome in the mouse brain Mouse brain proteins were digested with seven sets of proteases. The efficiencies of the proteases were compared while ZIC-HILIC was used as the enrichment technique (black arrow). Then, four enrichment techniques were evaluated on tryptic peptides (black arrow). Finally, the influence of fractionation before (with SCX) or after (with bRP) enrichment with ZIC-HILIC was assessed. For all of the above experiments, N-glycans were detached with PNGase F before online RPLC-MS/MS detection.

    Journal: Oncotarget

    Article Title: In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins

    doi: 10.18632/oncotarget.9737

    Figure Lengend Snippet: Experimental design for in-depth mapping of the N-glycoproteome in the mouse brain Mouse brain proteins were digested with seven sets of proteases. The efficiencies of the proteases were compared while ZIC-HILIC was used as the enrichment technique (black arrow). Then, four enrichment techniques were evaluated on tryptic peptides (black arrow). Finally, the influence of fractionation before (with SCX) or after (with bRP) enrichment with ZIC-HILIC was assessed. For all of the above experiments, N-glycans were detached with PNGase F before online RPLC-MS/MS detection.

    Article Snippet: ZIC-HILIC: Digested peptides (1 mg) dissolved in a loading buffer (80% CH3 CN and 1% CF3 COOH) were loaded onto a micro-column containing 30 mg ZIC-HILIC medium (5 μm, Merck Millipore, Darmstadt, Germany).

    Techniques: Hydrophilic Interaction Liquid Chromatography, Fractionation, Mass Spectrometry

    HILIC HPLC chromatogram of cPOS (Top) and cPOS-t (Bottom) at 235 nm. 6,7-dihydromonotropein ( a ), deacetylasperulsidic acid ( b ) and monotropein ( c ).

    Journal: Scientific Reports

    Article Title: Pectic Oligosaccharides from Cranberry Prevent Quiescence and Persistence in the Uropathogenic Escherichia coli CFT073

    doi: 10.1038/s41598-019-56005-w

    Figure Lengend Snippet: HILIC HPLC chromatogram of cPOS (Top) and cPOS-t (Bottom) at 235 nm. 6,7-dihydromonotropein ( a ), deacetylasperulsidic acid ( b ) and monotropein ( c ).

    Article Snippet: The 10% ACN/H2 O/0.1% TFA PGC eluent (cPOS, 15% w/w, dry) was then separated using a TSKgel Amide-80 HR HILIC column (4.6 × 250 mm, 5 μm, TOSOH Bioscience, Tokyo, Japan) at 1 mL/min and 35 °C.

    Techniques: Hydrophilic Interaction Liquid Chromatography, High Performance Liquid Chromatography

    Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and SFC Waters HILIC columns.

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Fast Reversed-Phase Liquid Chromatography to Reduce Back Exchange and Increase Throughput in H/D Exchange Monitored by FT-ICR Mass Spectrometry

    doi: 10.1016/j.jasms.2008.11.010

    Figure Lengend Snippet: Deuterium uptake for myoglobin peptides after exchange for 240 s and 900 s and separation with the Jupiter™ C 5 and ProZap™ C 18 columns with 1.5 min short gradient and SFC Waters HILIC columns.

    Article Snippet: SFC was conducted with a 4.6 mm × 50 mm Atlantis™ HILIC 5-μm silica column (Waters Corporation, Milford, MA), with a 4 min gradient from 80% to 40% CO2 at 3.5 mL/min.

    Techniques: Hydrophilic Interaction Liquid Chromatography

    Elution profiles for myoglobin fragment peptides through an SFC Waters HILIC column (top), HPLC ProZap™ C 18 column (middle) and HPLC Jupiter™ C 5 column (bottom), with respective retention times of ∼0.5-1.2 min, ∼0.7-1.5

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Fast Reversed-Phase Liquid Chromatography to Reduce Back Exchange and Increase Throughput in H/D Exchange Monitored by FT-ICR Mass Spectrometry

    doi: 10.1016/j.jasms.2008.11.010

    Figure Lengend Snippet: Elution profiles for myoglobin fragment peptides through an SFC Waters HILIC column (top), HPLC ProZap™ C 18 column (middle) and HPLC Jupiter™ C 5 column (bottom), with respective retention times of ∼0.5-1.2 min, ∼0.7-1.5

    Article Snippet: SFC was conducted with a 4.6 mm × 50 mm Atlantis™ HILIC 5-μm silica column (Waters Corporation, Milford, MA), with a 4 min gradient from 80% to 40% CO2 at 3.5 mL/min.

    Techniques: Hydrophilic Interaction Liquid Chromatography, High Performance Liquid Chromatography