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  • 85
    Hitachi Ltd high pressure liquid chromatography hplc separation
    High Pressure Liquid Chromatography Hplc Separation, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation high pressure liquid chromatography hplc separation
    High Pressure Liquid Chromatography Hplc Separation, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high pressure liquid chromatography hplc separations
    High Pressure Liquid Chromatography Hplc Separations, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co high pressure liquid chromatography hplc separation
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    Thermo Fisher high pressure liquid chromatography hplc
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    Agilent technologies high pressure liquid chromatograph
    High Pressure Liquid Chromatograph, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ultimate nanoscale rapid separation lc high pressure liquid chromatography nanorslc hplc system
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    Waters Corporation ultra high pressure liquid chromatography
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    Waters Corporation high pressure liquid chromatography hplc
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    High Pressure Liquid Chromatography Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sepax high pressure liquid chromatography hplc column
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    High Pressure Liquid Chromatography Hplc Column, supplied by Sepax, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer high pressure liquid chromatography system
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    High Pressure Liquid Chromatography System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hewlett-Packard reversed phase high pressure liquid chromatography
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    Reversed Phase High Pressure Liquid Chromatography, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 89/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 3000 uhp nano rapid separation liquid chromatography series high pressure liquid chromatography system
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    3000 Uhp Nano Rapid Separation Liquid Chromatography Series High Pressure Liquid Chromatography System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reversed phase high pressure liquid chromatography
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    Reversed Phase High Pressure Liquid Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation reverse phase high pressure liquid chromatography
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    Reverse Phase High Pressure Liquid Chromatography, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shimadzu Corporation high pressure liquid chromatography separation
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    High Pressure Liquid Chromatography Separation, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 1100 high pressure liquid chromatography system
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    1100 High Pressure Liquid Chromatography System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high pressure liquid chromatography
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    High Pressure Liquid Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    JASCO Inc high pressure liquid chromatography
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    High Pressure Liquid Chromatography, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nanoacquity ultra high pressure liquid chromatography system
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    Nanoacquity Ultra High Pressure Liquid Chromatography System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation high pressure liquid chromatograph hplc
    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with <t>AccQ-Fluor,</t> and fractionated over a hydrolysate amino acid analysis <t>HPLC</t> column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.
    High Pressure Liquid Chromatograph Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare high pressure liquid chromatography hplc
    <t>HPLC</t> analysis of phosphorylated [ 3 <t>H]GCV</t> products from TK- and PT-expressing cells. (A) 293T cells transfected with the vector control, ORF 21, or ORF 36-expressing plasmids were incubated with 8 μM [ 3 H]GCV (specific activity, 1,000 dpm/pmol) for 30 h. Phosphorylated nucleosides were extracted and analyzed by HPLC (expressed in picomoles of GCV/10 6 cells [determined for each sample]). Histograms show GCV MP, GCV DP, and GCV TP levels in the vector, TK, and PT transfectants relative to untransfected 293T control cells. Data shown are the averages of triplicate measurements in single experiments. Each experiment was repeated three times, and results were consistent. Error bars indicate standard deviations. (B) Western blot analysis was performed on protein extracts from TK and PT transfectants by using an anti-HA antibody specific for HA fusion tags at the N terminus of expressed TK and PT proteins. Efficiencies of transfection for each sample were corrected with an internal β-galactosidase control. The HA antibody shows cross-reactivity to a 90- to 100-kDa protein present in all 293T cells tested.
    High Pressure Liquid Chromatography Hplc, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation acquity ultra high pressure liquid chromatography system
    <t>HPLC</t> analysis of phosphorylated [ 3 <t>H]GCV</t> products from TK- and PT-expressing cells. (A) 293T cells transfected with the vector control, ORF 21, or ORF 36-expressing plasmids were incubated with 8 μM [ 3 H]GCV (specific activity, 1,000 dpm/pmol) for 30 h. Phosphorylated nucleosides were extracted and analyzed by HPLC (expressed in picomoles of GCV/10 6 cells [determined for each sample]). Histograms show GCV MP, GCV DP, and GCV TP levels in the vector, TK, and PT transfectants relative to untransfected 293T control cells. Data shown are the averages of triplicate measurements in single experiments. Each experiment was repeated three times, and results were consistent. Error bars indicate standard deviations. (B) Western blot analysis was performed on protein extracts from TK and PT transfectants by using an anti-HA antibody specific for HA fusion tags at the N terminus of expressed TK and PT proteins. Efficiencies of transfection for each sample were corrected with an internal β-galactosidase control. The HA antibody shows cross-reactivity to a 90- to 100-kDa protein present in all 293T cells tested.
    Acquity Ultra High Pressure Liquid Chromatography System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high pressure liquid chromatography
    <t>HPLC</t> analysis of phosphorylated [ 3 <t>H]GCV</t> products from TK- and PT-expressing cells. (A) 293T cells transfected with the vector control, ORF 21, or ORF 36-expressing plasmids were incubated with 8 μM [ 3 H]GCV (specific activity, 1,000 dpm/pmol) for 30 h. Phosphorylated nucleosides were extracted and analyzed by HPLC (expressed in picomoles of GCV/10 6 cells [determined for each sample]). Histograms show GCV MP, GCV DP, and GCV TP levels in the vector, TK, and PT transfectants relative to untransfected 293T control cells. Data shown are the averages of triplicate measurements in single experiments. Each experiment was repeated three times, and results were consistent. Error bars indicate standard deviations. (B) Western blot analysis was performed on protein extracts from TK and PT transfectants by using an anti-HA antibody specific for HA fusion tags at the N terminus of expressed TK and PT proteins. Efficiencies of transfection for each sample were corrected with an internal β-galactosidase control. The HA antibody shows cross-reactivity to a 90- to 100-kDa protein present in all 293T cells tested.
    High Pressure Liquid Chromatography, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with AccQ-Fluor, and fractionated over a hydrolysate amino acid analysis HPLC column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.

    Journal: Journal of Bacteriology

    Article Title: Spermidine Inversely Influences Surface Interactions and Planktonic Growth in Agrobacterium tumefaciens

    doi: 10.1128/JB.00265-16

    Figure Lengend Snippet: Polyamine profiles from A. tumefaciens mutants for CASDH and homospermidine synthase ( hss ). Polyamines were prepared from whole-cell extracts, labeled with AccQ-Fluor, and fractionated over a hydrolysate amino acid analysis HPLC column with an acetonitrile gradient. Polyamine and ornithine peaks were detected using an in-line fluorescence detector (excitation, 248 nm; emission, 398 nm). Put, putrescine; Fl, unconjugated AccQ-Fluor; Spd, spermidine; Hspd, homospermidine.

    Article Snippet: Polyamines labeled with the AccQ-Fluor reagent were separated by high-pressure liquid chromatography (HPLC) with a hydrolysate amino acid analysis column (AccQ-Tag column; 60 Å, 4 μm, 3.9 by 150 mm; Waters) on a Beckman Coulter System Gold with fluorescence detection (excitation, 248 nm; emission, 398 nm [ProStar]).

    Techniques: Labeling, High Performance Liquid Chromatography, Fluorescence

    HPLC analysis of phosphorylated [ 3 H]GCV products from TK- and PT-expressing cells. (A) 293T cells transfected with the vector control, ORF 21, or ORF 36-expressing plasmids were incubated with 8 μM [ 3 H]GCV (specific activity, 1,000 dpm/pmol) for 30 h. Phosphorylated nucleosides were extracted and analyzed by HPLC (expressed in picomoles of GCV/10 6 cells [determined for each sample]). Histograms show GCV MP, GCV DP, and GCV TP levels in the vector, TK, and PT transfectants relative to untransfected 293T control cells. Data shown are the averages of triplicate measurements in single experiments. Each experiment was repeated three times, and results were consistent. Error bars indicate standard deviations. (B) Western blot analysis was performed on protein extracts from TK and PT transfectants by using an anti-HA antibody specific for HA fusion tags at the N terminus of expressed TK and PT proteins. Efficiencies of transfection for each sample were corrected with an internal β-galactosidase control. The HA antibody shows cross-reactivity to a 90- to 100-kDa protein present in all 293T cells tested.

    Journal: Journal of Virology

    Article Title: Human Herpesvirus 8-Encoded Thymidine Kinase and Phosphotransferase Homologues Confer Sensitivity to Ganciclovir

    doi:

    Figure Lengend Snippet: HPLC analysis of phosphorylated [ 3 H]GCV products from TK- and PT-expressing cells. (A) 293T cells transfected with the vector control, ORF 21, or ORF 36-expressing plasmids were incubated with 8 μM [ 3 H]GCV (specific activity, 1,000 dpm/pmol) for 30 h. Phosphorylated nucleosides were extracted and analyzed by HPLC (expressed in picomoles of GCV/10 6 cells [determined for each sample]). Histograms show GCV MP, GCV DP, and GCV TP levels in the vector, TK, and PT transfectants relative to untransfected 293T control cells. Data shown are the averages of triplicate measurements in single experiments. Each experiment was repeated three times, and results were consistent. Error bars indicate standard deviations. (B) Western blot analysis was performed on protein extracts from TK and PT transfectants by using an anti-HA antibody specific for HA fusion tags at the N terminus of expressed TK and PT proteins. Efficiencies of transfection for each sample were corrected with an internal β-galactosidase control. The HA antibody shows cross-reactivity to a 90- to 100-kDa protein present in all 293T cells tested.

    Article Snippet: Phosphorylated forms of GCV were separated by high-pressure liquid chromatography (HPLC) with a strong anion-exchange column (Whatman Partisil 10-SAX) according to a previously described procedure with minor modifications ( , ).

    Techniques: High Performance Liquid Chromatography, Expressing, Transfection, Plasmid Preparation, Incubation, Activity Assay, Western Blot