high-performance liquid chromatography hplc condition Search Results


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  • 99
    Thermo Fisher ultimate 3000 hplc system
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    Agilent technologies 1200 hplc system
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    Agilent technologies hplc condition
    The <t>HPLC</t> method for <t>ENR</t> quantification in plasma . The representative HPLC chromatograms of plasma were shown: (A) blank plasma sample; (B) plasma sample at the LLOQ of 0.05 μg/ml; (C) plasma sample after oral administration of EEG at the point of 1 h; ENR, ENR at the peak time of 13.5 min.
    Hplc Condition, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 5567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 1100 hplc system
    The <t>HPLC</t> method for <t>ENR</t> quantification in plasma . The representative HPLC chromatograms of plasma were shown: (A) blank plasma sample; (B) plasma sample at the LLOQ of 0.05 μg/ml; (C) plasma sample after oral administration of EEG at the point of 1 h; ENR, ENR at the peak time of 13.5 min.
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    Agilent technologies 1260 hplc system
    The <t>HPLC</t> method for <t>ENR</t> quantification in plasma . The representative HPLC chromatograms of plasma were shown: (A) blank plasma sample; (B) plasma sample at the LLOQ of 0.05 μg/ml; (C) plasma sample after oral administration of EEG at the point of 1 h; ENR, ENR at the peak time of 13.5 min.
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    Waters Corporation 2695 hplc system
    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters <t>2695</t> <t>HPLC</t> system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).
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    Waters Corporation acquity uplc hss t3 c18
    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters <t>2695</t> <t>HPLC</t> system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).
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    Waters Corporation hplc analysis
    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters <t>2695</t> <t>HPLC</t> system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).
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    Agilent technologies chromatographic conditions hplc analysis
    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters <t>2695</t> <t>HPLC</t> system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).
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    Waters Corporation 2695 alliance hplc system
    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters <t>2695</t> <t>HPLC</t> system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).
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    JASCO Inc hplc system
    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters <t>2695</t> <t>HPLC</t> system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).
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    Agilent technologies 1260 infinity hplc system
    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters <t>2695</t> <t>HPLC</t> system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).
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    Waters Corporation chromatographic conditions hplc
    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters <t>2695</t> <t>HPLC</t> system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).
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    HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus <t>C18</t> column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.
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    Image Search Results


    The HPLC method for ENR quantification in plasma . The representative HPLC chromatograms of plasma were shown: (A) blank plasma sample; (B) plasma sample at the LLOQ of 0.05 μg/ml; (C) plasma sample after oral administration of EEG at the point of 1 h; ENR, ENR at the peak time of 13.5 min.

    Journal: Frontiers in Pharmacology

    Article Title: Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

    doi: 10.3389/fphar.2017.00294

    Figure Lengend Snippet: The HPLC method for ENR quantification in plasma . The representative HPLC chromatograms of plasma were shown: (A) blank plasma sample; (B) plasma sample at the LLOQ of 0.05 μg/ml; (C) plasma sample after oral administration of EEG at the point of 1 h; ENR, ENR at the peak time of 13.5 min.

    Article Snippet: HPLC condition of ENR and CP and pharmacokinetic analysis A C18 reverse-phase column (250 × 4.6 mm, i.d., 5 μm, Agilent, USA) was used for HPLC, which was performed with a 278 nm detection wavelength at 30°C.

    Techniques: High Performance Liquid Chromatography

    The HPLC methods for ENR quantification in tissues . The representative HPLC chromatograms of tissues were shown: (A,C,E,G) represent the blank samples in muscle, fat, liver, and kidney, respectively. (B,D,F,H) represent the LLOQ of 0.05 μg/ml in muscle, fat, liver, and kidney, respectively.

    Journal: Frontiers in Pharmacology

    Article Title: Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs

    doi: 10.3389/fphar.2017.00294

    Figure Lengend Snippet: The HPLC methods for ENR quantification in tissues . The representative HPLC chromatograms of tissues were shown: (A,C,E,G) represent the blank samples in muscle, fat, liver, and kidney, respectively. (B,D,F,H) represent the LLOQ of 0.05 μg/ml in muscle, fat, liver, and kidney, respectively.

    Article Snippet: HPLC condition of ENR and CP and pharmacokinetic analysis A C18 reverse-phase column (250 × 4.6 mm, i.d., 5 μm, Agilent, USA) was used for HPLC, which was performed with a 278 nm detection wavelength at 30°C.

    Techniques: High Performance Liquid Chromatography

    Diclofenac concentrations in the serum (black columns) and the synovial washing fluid (gray columns) measured by HPLC (Series 1). Notes: Diclofenac sodium gel was applied topically above the knee joint (topical), or EP was applied for 8 min over the knee joint (EP) after the diclofenac gel dispersed. Samples of blood and synovial washing fluid were collected 10 min after application. Data are presented as means ± SD. # p

    Journal: Drug Design, Development and Therapy

    Article Title: Electroporation-enhanced transdermal diclofenac sodium delivery into the knee joint in a rat model of acute arthritis

    doi: 10.2147/DDDT.S161703

    Figure Lengend Snippet: Diclofenac concentrations in the serum (black columns) and the synovial washing fluid (gray columns) measured by HPLC (Series 1). Notes: Diclofenac sodium gel was applied topically above the knee joint (topical), or EP was applied for 8 min over the knee joint (EP) after the diclofenac gel dispersed. Samples of blood and synovial washing fluid were collected 10 min after application. Data are presented as means ± SD. # p

    Article Snippet: HPLC system and conditions The HPLC analysis of diclofenac sodium was conducted using an Agilent HPLC system (Agilent Technologies, Palo Alto, CA, USA) equipped with an automated solvent delivery system, which has an integrated degasser (1260 Infinity Quaternary Pump, Agilent Technologies), an Agilent 1260 Infinity autosampler, and a 1024-element diode array detector (1260 Infinity Diode Array Detector, Agilent Technologies).

    Techniques: High Performance Liquid Chromatography

    Time sequence of interventions. Notes: In the first series, the diclofenac concentration in the serum and the synovial washing fluid was measured by HPLC. In the second experimental series, tests for secondary mechanical touch sensitivity and heat-provoked paw withdrawal were performed 24 h after arthritis induction with C/K and for knee joint swelling measurements 48 h after the challenge. In the third series, IVM examinations of the synovial membrane were performed 6 h after the challenge. Abbreviations: C/K, carrageenan/kaolin; D, diclofenac treatment; HPLC, high-performance liquid chromatography; IVM, intravital videomicroscopy; S, sample taken.

    Journal: Drug Design, Development and Therapy

    Article Title: Electroporation-enhanced transdermal diclofenac sodium delivery into the knee joint in a rat model of acute arthritis

    doi: 10.2147/DDDT.S161703

    Figure Lengend Snippet: Time sequence of interventions. Notes: In the first series, the diclofenac concentration in the serum and the synovial washing fluid was measured by HPLC. In the second experimental series, tests for secondary mechanical touch sensitivity and heat-provoked paw withdrawal were performed 24 h after arthritis induction with C/K and for knee joint swelling measurements 48 h after the challenge. In the third series, IVM examinations of the synovial membrane were performed 6 h after the challenge. Abbreviations: C/K, carrageenan/kaolin; D, diclofenac treatment; HPLC, high-performance liquid chromatography; IVM, intravital videomicroscopy; S, sample taken.

    Article Snippet: HPLC system and conditions The HPLC analysis of diclofenac sodium was conducted using an Agilent HPLC system (Agilent Technologies, Palo Alto, CA, USA) equipped with an automated solvent delivery system, which has an integrated degasser (1260 Infinity Quaternary Pump, Agilent Technologies), an Agilent 1260 Infinity autosampler, and a 1024-element diode array detector (1260 Infinity Diode Array Detector, Agilent Technologies).

    Techniques: Sequencing, Concentration Assay, High Performance Liquid Chromatography

    Chromatograms of DPDFT in plasma sample. Separation was performed using Waters 2695 HPLC system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).

    Journal: Bioinorganic Chemistry and Applications

    Article Title: Pharmacokinetic Study of Di-Phenyl-Di-(2,4-Difluobenzohydroxamato)Tin(IV): Novel Metal-Based Complex with Promising Antitumor Potential

    doi: 10.1155/2012/210682

    Figure Lengend Snippet: Chromatograms of DPDFT in plasma sample. Separation was performed using Waters 2695 HPLC system. The mobile phase consisted of phosphoric acid in water (solvent A)/methanol (solvent B) (30 : 70, V/V, pH 3.0) using Diamonsil C 18 column at 25°C with a flow rate of 0.8 mL·min −1 . (a) Blank plasma; (b) blank plasma spiked with DPDFT (3 μ g·mL −1 ) and the I.S. (8 μ g·mL −1 ); (c) blood sample containing DPDFT (2.4 μ g·mL −1 ) collected at 3 min after administration of DPDFT (15 mg·kg −1 , i.v.).

    Article Snippet: Instrumentation and Chromatographic Conditions HPLC analysis was carried out using a Waters 2695 HPLC system (Waters Associates, Milford, MA) which consisted of a photodiode array detector, an autosampler, and a degasser.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry

    HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.

    Journal: Journal of labelled compounds & radiopharmaceuticals

    Article Title: Fast Indirect Fluorine-18 Labeling of Protein/Peptide using the useful 6-Fluoronicotinic acid-2,3,5,6-Tetrafluorophenyl prosthetic group: A Method Comparable to direct Fluorination

    doi: 10.1002/jlcr.3487

    Figure Lengend Snippet: HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.

    Article Snippet: HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm).

    Techniques: High Performance Liquid Chromatography, Purification, Injection, Flow Cytometry