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  • 99
    Millipore pressure liquid chromatography hplc
    <t>HPLC</t> chromatographs of <t>mCP.</t> (a) mCP direct from vendor (Aldrich). Absorbances versus elution time were obtained using a broadband light source. The small inset panels show the UV–vis spectra (200 to 600 nm) of indicator components eluted at different times. (b) Purified mCP.
    Pressure Liquid Chromatography Hplc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pressure liquid chromatography hplc/product/Millipore
    Average 99 stars, based on 160 article reviews
    Price from $9.99 to $1999.99
    pressure liquid chromatography hplc - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore high pressure liquid chromatography
    <t>HPLC</t> chromatographs of <t>mCP.</t> (a) mCP direct from vendor (Aldrich). Absorbances versus elution time were obtained using a broadband light source. The small inset panels show the UV–vis spectra (200 to 600 nm) of indicator components eluted at different times. (b) Purified mCP.
    High Pressure Liquid Chromatography, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high pressure liquid chromatography/product/Millipore
    Average 99 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    high pressure liquid chromatography - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Tosoh Corporation high performance pressure liquid chromatography
    <t>HPLC</t> chromatographs of <t>mCP.</t> (a) mCP direct from vendor (Aldrich). Absorbances versus elution time were obtained using a broadband light source. The small inset panels show the UV–vis spectra (200 to 600 nm) of indicator components eluted at different times. (b) Purified mCP.
    High Performance Pressure Liquid Chromatography, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance pressure liquid chromatography/product/Tosoh Corporation
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance pressure liquid chromatography - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    HPLC chromatographs of mCP. (a) mCP direct from vendor (Aldrich). Absorbances versus elution time were obtained using a broadband light source. The small inset panels show the UV–vis spectra (200 to 600 nm) of indicator components eluted at different times. (b) Purified mCP.

    Journal: Environmental Science & Technology

    Article Title: Purification and Characterization of meta-Cresol Purple for Spectrophotometric Seawater pH Measurements

    doi: 10.1021/es200665d

    Figure Lengend Snippet: HPLC chromatographs of mCP. (a) mCP direct from vendor (Aldrich). Absorbances versus elution time were obtained using a broadband light source. The small inset panels show the UV–vis spectra (200 to 600 nm) of indicator components eluted at different times. (b) Purified mCP.

    Article Snippet: Figure a shows an HPLC chromatograph of an unpurified Aldrich mCP sample on a Primesep B2 column.

    Techniques: High Performance Liquid Chromatography, Purification

    Comparison of insulin B chain cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.

    Journal: PLoS ONE

    Article Title: Active Site Mutations Change the Cleavage Specificity of Neprilysin

    doi: 10.1371/journal.pone.0032343

    Figure Lengend Snippet: Comparison of insulin B chain cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.

    Article Snippet: HPLC assays Cleavage of physiological peptides was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of the purified NEP or its mutants with 15 µM insulin B chain (Sigma Aldrich), 24 µM Aß1–40 (Anaspec), or 64 µM leu-ENK (Sigma) in 100 µL of 20 mM MES, pH 6.5, at 37°C.

    Techniques: High Performance Liquid Chromatography, Mutagenesis

    Comparison of Aß 1–40 cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC cleavage profile of Aß 1–40 cleavage by NEP and NEP mutants at ∼30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of Aß 1–40 from Table 2 . Reactions were carried out at 37°C with 15 µM Aß 1–40 in 20 mM MES, pH 6.5.

    Journal: PLoS ONE

    Article Title: Active Site Mutations Change the Cleavage Specificity of Neprilysin

    doi: 10.1371/journal.pone.0032343

    Figure Lengend Snippet: Comparison of Aß 1–40 cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC cleavage profile of Aß 1–40 cleavage by NEP and NEP mutants at ∼30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of Aß 1–40 from Table 2 . Reactions were carried out at 37°C with 15 µM Aß 1–40 in 20 mM MES, pH 6.5.

    Article Snippet: HPLC assays Cleavage of physiological peptides was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of the purified NEP or its mutants with 15 µM insulin B chain (Sigma Aldrich), 24 µM Aß1–40 (Anaspec), or 64 µM leu-ENK (Sigma) in 100 µL of 20 mM MES, pH 6.5, at 37°C.

    Techniques: High Performance Liquid Chromatography, Mutagenesis