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  • 99
    Thermo Fisher high performance liquid chromatography hplc
    High Performance Liquid Chromatography Hplc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore hplc
    Hplc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3656 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Agilent technologies high performance liquid chromatography hplc
    <t>HBPH-dependent</t> growth of Microbacterium sp. HM58-2. Microbacterium sp. HM58-2 was cultured in MM-HBPH medium at 30°C. Concentrations of HBPH (filled circles) and acetophenone hydrazone (open circles) were monitored by <t>HPLC.</t> The concentration of
    High Performance Liquid Chromatography Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1865 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Waters Corporation high performance liquid chromatography hplc
    <t>HPLC</t> of chromatogram of (A) standard marker compounds (AGP, <t>NAG,</t> and DDAG, 0.1 mg/mL) found in AP and (B) standardised APAE.
    High Performance Liquid Chromatography Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shimadzu Corporation high performance liquid chromatography hplc
    <t>HPLC/DAD</t> chromatogram of the acetyl acetate extract of pomegranate leaves (EAFPg) monitored at 327 nm (a). Structure of constituents identified by HPLC-DAD-ESI-IT/MS (b). ID: identification; RT: retention time; MS: mass spectrometer.
    High Performance Liquid Chromatography Hplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad high performance liquid chromatography hplc
    <t>HPLC/DAD</t> chromatogram of the acetyl acetate extract of pomegranate leaves (EAFPg) monitored at 327 nm (a). Structure of constituents identified by HPLC-DAD-ESI-IT/MS (b). ID: identification; RT: retention time; MS: mass spectrometer.
    High Performance Liquid Chromatography Hplc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies 1100 high performance liquid chromatography hplc
    HBXIP enhances the glucose metabolism reprogramming of breast cancer cells through miR-183/96/182 in vitro A. The levels of lactate in the culture media of MCF-7 cells were measured by an Agilent <t>1100</t> series high-performance liquid chromatography <t>(HPLC)</t> system and normalized to cell number. B. The levels of intracellular glucose were detected by glucose-lactate biosense tester SBA-40E and normalized based on the protein concentration in MCF-7 cells. C. The levels of intracellular ROS were assessed by flow cytometry analysis in MCF-7 cells. D–F. The relative levels of lactate, intracellular glucose and ROS were measured using above methods in HBXIP-transfected MCF-7 cells treated with anti-miR-183 (anti-miR-96 or anti-miR-182). Statistically significant differences are indicated: * P
    1100 High Performance Liquid Chromatography Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies 1200 series high performance liquid chromatography hplc
    ( a ) Representative <t>HPLC-MS</t> chromatogram (HILIC-ESI-MS) from site U1357, 7.15 mbsf, showing the presence of major intact polar lipid isoprenoidal glycerol dialkyl glycerol tetraethers (IPL-GDGTs) and bacterial lipids in the mass range m/z <t>1200–1800.</t> The HPLC-MS chromatogram is depicted as a density map showing retention time on the x axis, m/z on the y axis and the relative peak intensity by grey shading. ( b ) Structural variety in the head groups and biphytanyl moieties of the detected IPL-GDGTs. Note that during the ether cleavage reaction the hydroxyl group in the biphytane (bp) side chain becomes dehydrated and forms biphytene (bp0:1). Position of the hydroxyl group and double bond were not determined. 1G, monohexose; 2G, dihexose; PG, phosphatidyl glycerol; HPH, hexose-phoshohexose; cren, crenarchaeol.
    1200 Series High Performance Liquid Chromatography Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Hitachi Ltd high performance liquid chromatography hplc
    ( a ) Representative <t>HPLC-MS</t> chromatogram (HILIC-ESI-MS) from site U1357, 7.15 mbsf, showing the presence of major intact polar lipid isoprenoidal glycerol dialkyl glycerol tetraethers (IPL-GDGTs) and bacterial lipids in the mass range m/z <t>1200–1800.</t> The HPLC-MS chromatogram is depicted as a density map showing retention time on the x axis, m/z on the y axis and the relative peak intensity by grey shading. ( b ) Structural variety in the head groups and biphytanyl moieties of the detected IPL-GDGTs. Note that during the ether cleavage reaction the hydroxyl group in the biphytane (bp) side chain becomes dehydrated and forms biphytene (bp0:1). Position of the hydroxyl group and double bond were not determined. 1G, monohexose; 2G, dihexose; PG, phosphatidyl glycerol; HPH, hexose-phoshohexose; cren, crenarchaeol.
    High Performance Liquid Chromatography Hplc, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 93/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Phenomenex high performance liquid chromatography hplc
    ( a ) Representative <t>HPLC-MS</t> chromatogram (HILIC-ESI-MS) from site U1357, 7.15 mbsf, showing the presence of major intact polar lipid isoprenoidal glycerol dialkyl glycerol tetraethers (IPL-GDGTs) and bacterial lipids in the mass range m/z <t>1200–1800.</t> The HPLC-MS chromatogram is depicted as a density map showing retention time on the x axis, m/z on the y axis and the relative peak intensity by grey shading. ( b ) Structural variety in the head groups and biphytanyl moieties of the detected IPL-GDGTs. Note that during the ether cleavage reaction the hydroxyl group in the biphytane (bp) side chain becomes dehydrated and forms biphytene (bp0:1). Position of the hydroxyl group and double bond were not determined. 1G, monohexose; 2G, dihexose; PG, phosphatidyl glycerol; HPH, hexose-phoshohexose; cren, crenarchaeol.
    High Performance Liquid Chromatography Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 92/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific high performance liquid chromatography hplc grade
    FAE quantitative <t>HPLC</t> assay of free (free L.f. 5221) and microencapsulated L. fermentum NCIMB 5221 (APA L.f. 5221). Uninoculated <t>MRS-EFA</t> was used as a negative control. The presented data represents the amount of unhydrolysed EFA remaining in solution ( A ) and the amount of FA produced ( B ), as measured by HPLC peak area data. Each point represents the mean of triplicates and the error bars represent the standard deviations.These results demonstrate no significant difference in FA production between the free and encapsulated L. fermentum NCIMB 5221.
    High Performance Liquid Chromatography Hplc Grade, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HBPH-dependent growth of Microbacterium sp. HM58-2. Microbacterium sp. HM58-2 was cultured in MM-HBPH medium at 30°C. Concentrations of HBPH (filled circles) and acetophenone hydrazone (open circles) were monitored by HPLC. The concentration of

    Journal: Journal of Bacteriology

    Article Title: Hydrazidase, a Novel Amidase Signature Enzyme That Hydrolyzes Acylhydrazides

    doi: 10.1128/JB.02443-14

    Figure Lengend Snippet: HBPH-dependent growth of Microbacterium sp. HM58-2. Microbacterium sp. HM58-2 was cultured in MM-HBPH medium at 30°C. Concentrations of HBPH (filled circles) and acetophenone hydrazone (open circles) were monitored by HPLC. The concentration of

    Article Snippet: The HBPH concentration was determined using a high-performance liquid chromatography (HPLC) system (HP-1100; Agilent Technologies, Santa Clara, CA) equipped with an ODS-4 column (4.6 by 150 mm) containing Inertsil (particle diameter, 3 μl) and by monitoring absorption at 210 nm.

    Techniques: Cell Culture, High Performance Liquid Chromatography, Concentration Assay

    H 2 S deficiency increased the expression and activity of IDO1 in Cse −/− mice. a HPLC analysis of Kyn/Trp ratios in wild-type (wt) mice and Cse −/− mice, N = 6. b , c Analysis of IDO1 expression in different tissues of wt mice and Cse −/− mice. Total RNA and protein were extracted from different tissues of wt mice and Cse −/− mice. mRNA expression of Ido1 was analyzed by qPCR, values from three independent experiments are presented as the mean ± SEM, * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: H2S suppresses indoleamine 2, 3-dioxygenase 1 and exhibits immunotherapeutic efficacy in murine hepatocellular carcinoma

    doi: 10.1186/s13046-019-1083-5

    Figure Lengend Snippet: H 2 S deficiency increased the expression and activity of IDO1 in Cse −/− mice. a HPLC analysis of Kyn/Trp ratios in wild-type (wt) mice and Cse −/− mice, N = 6. b , c Analysis of IDO1 expression in different tissues of wt mice and Cse −/− mice. Total RNA and protein were extracted from different tissues of wt mice and Cse −/− mice. mRNA expression of Ido1 was analyzed by qPCR, values from three independent experiments are presented as the mean ± SEM, * p

    Article Snippet: High-performance liquid chromatography (HPLC) analysis The IDO1 activities of mouse serum and cell supernatant were analyzed by an Agilent 1260 series HPLC system (Agilent Corp., USA).

    Techniques: Expressing, Activity Assay, Mouse Assay, High Performance Liquid Chromatography, Real-time Polymerase Chain Reaction

    H 2 S inhibited IDO1 activity via H 2 S/NO crosstalk, and NO rather than H 2 S was an IDO1 inhibitor. a H 2 S donor upregulated the mRNA expression of iNOS in MCF-7 and SGC-7901 cells. Total RNA was extracted from the cells that were treated with different concentrations of NaHS for 24 h, and the mRNA expression levels of iNOS were analyzed by qPCR. b H 2 S deficiency downregulated the mRNA expression of Inos in Cse −/− mice. Total RNA was extracted from different tissues of the wt mice and Cse −/− mice, and the mRNA expression levels of Inos were analyzed by qPCR. c NO played an important role in the regulation of H 2 S on IDO1 activity. The cell supernatants were harvested, and kynurenine and tryptophan levels were measured by HPLC. d , e NO rather than H 2 S was a direct inhibitor of IDO1. Enzymatic assay of IDO1 was carried out with recombinant human IDO1 (rhIDO1) and different concentrations (0–400 μM) of H 2 S donors (NaHS, GYY4137), NO donor (SNP) and 1-L-MT. f Schematic diagram depicting the modulation of H 2 S on IDO1. All experiments repeated at least three times. Statistical significance was determined by Student’s t test ( b ) and one-way ANOVA followed by Dunnett’s test ( a , c and d ). * P

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: H2S suppresses indoleamine 2, 3-dioxygenase 1 and exhibits immunotherapeutic efficacy in murine hepatocellular carcinoma

    doi: 10.1186/s13046-019-1083-5

    Figure Lengend Snippet: H 2 S inhibited IDO1 activity via H 2 S/NO crosstalk, and NO rather than H 2 S was an IDO1 inhibitor. a H 2 S donor upregulated the mRNA expression of iNOS in MCF-7 and SGC-7901 cells. Total RNA was extracted from the cells that were treated with different concentrations of NaHS for 24 h, and the mRNA expression levels of iNOS were analyzed by qPCR. b H 2 S deficiency downregulated the mRNA expression of Inos in Cse −/− mice. Total RNA was extracted from different tissues of the wt mice and Cse −/− mice, and the mRNA expression levels of Inos were analyzed by qPCR. c NO played an important role in the regulation of H 2 S on IDO1 activity. The cell supernatants were harvested, and kynurenine and tryptophan levels were measured by HPLC. d , e NO rather than H 2 S was a direct inhibitor of IDO1. Enzymatic assay of IDO1 was carried out with recombinant human IDO1 (rhIDO1) and different concentrations (0–400 μM) of H 2 S donors (NaHS, GYY4137), NO donor (SNP) and 1-L-MT. f Schematic diagram depicting the modulation of H 2 S on IDO1. All experiments repeated at least three times. Statistical significance was determined by Student’s t test ( b ) and one-way ANOVA followed by Dunnett’s test ( a , c and d ). * P

    Article Snippet: High-performance liquid chromatography (HPLC) analysis The IDO1 activities of mouse serum and cell supernatant were analyzed by an Agilent 1260 series HPLC system (Agilent Corp., USA).

    Techniques: Activity Assay, Expressing, Real-time Polymerase Chain Reaction, Mouse Assay, High Performance Liquid Chromatography, Enzymatic Assay, Recombinant

    HPLC of chromatogram of (A) standard marker compounds (AGP, NAG, and DDAG, 0.1 mg/mL) found in AP and (B) standardised APAE.

    Journal: Journal of Advanced Research

    Article Title: A standardised Andrographis paniculata Burm. Nees aqueous extract prevents Lipopolysaccharide-induced cognitive deficits through suppression of inflammatory cytokines and oxidative stress mediators

    doi: 10.1016/j.jare.2018.11.005

    Figure Lengend Snippet: HPLC of chromatogram of (A) standard marker compounds (AGP, NAG, and DDAG, 0.1 mg/mL) found in AP and (B) standardised APAE.

    Article Snippet: Thereafter, the extract was standardised using a Waters high-performance liquid chromatography (HPLC) system to andrographolide (AGP), neoandrographolide (NAG), and 14-deoxy-11, 12-didehydroandrographolide (DDAG) contents, and the standardised extract was termed APAE and used for the study.

    Techniques: High Performance Liquid Chromatography, Marker

    HPLC chromatogram for DAE (A), DHE (B) standard Betulinic acid (C). Arrow indicates peak for betulinic acid.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Dillenia indica L. attenuates diabetic nephropathy via inhibition of advanced glycation end products accumulation in STZ-nicotinamide induced diabetic rats

    doi: 10.1016/j.jtcme.2017.06.004

    Figure Lengend Snippet: HPLC chromatogram for DAE (A), DHE (B) standard Betulinic acid (C). Arrow indicates peak for betulinic acid.

    Article Snippet: 2.6 Quantification of betulinic acid by using HPLC Betulinic acid was quantified in DAE and DHE by high-performance liquid chromatography (HPLC) analysis using a Waters 2795 HPLC system with a UV detector.

    Techniques: High Performance Liquid Chromatography

    HPLC/DAD chromatogram of the acetyl acetate extract of pomegranate leaves (EAFPg) monitored at 327 nm (a). Structure of constituents identified by HPLC-DAD-ESI-IT/MS (b). ID: identification; RT: retention time; MS: mass spectrometer.

    Journal: Journal of Immunology Research

    Article Title: Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury

    doi: 10.1155/2018/6879183

    Figure Lengend Snippet: HPLC/DAD chromatogram of the acetyl acetate extract of pomegranate leaves (EAFPg) monitored at 327 nm (a). Structure of constituents identified by HPLC-DAD-ESI-IT/MS (b). ID: identification; RT: retention time; MS: mass spectrometer.

    Article Snippet: HPLC-DAD-ESI-IT/MS Analysis The chemical constituents of EAFPg were analyzed in a high-performance liquid chromatography (HPLC) system (LC-10AD, Shimadzu) equipped with a photodiode array detector coupled to an Esquire 3000 Plus ion trap mass spectrometer (Bruker Daltonics, Bremen, Germany), using electrospray ionization (ESI) as previously described [ ].

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Three-dimensional chromatogram of GMSYS by HPLC-PDA. The retention times of 11 marker compounds—gallic acid, neomangiferin, chlorogenic acid, mangiferin, geniposide, paeoniflorin, berberine, liquiritin, nodakenin, glycyrrhizin, and atractylenolide III—were approximately 6.32, 10.82, 12.63, 13.04, 13.53, 15.17, 15.35, 16.61, 17.27, 29.86, and 34.00 min, respectively

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Anti-inflammatory effect and action mechanisms of traditional herbal formula Gamisoyo-san in RAW 264.7 macrophages

    doi: 10.1186/s12906-016-1197-7

    Figure Lengend Snippet: Three-dimensional chromatogram of GMSYS by HPLC-PDA. The retention times of 11 marker compounds—gallic acid, neomangiferin, chlorogenic acid, mangiferin, geniposide, paeoniflorin, berberine, liquiritin, nodakenin, glycyrrhizin, and atractylenolide III—were approximately 6.32, 10.82, 12.63, 13.04, 13.53, 15.17, 15.35, 16.61, 17.27, 29.86, and 34.00 min, respectively

    Article Snippet: High-performance liquid chromatography (HPLC) analysis of GMSYS Quantitative analysis of the GMSYS sample was performed using an LC-20A Prominence HPLC system (Shimadzu Corp., Kyoto, Japan) equipped with a solvent delivery unit, an on-line degasser, a column oven, an autosampler, and a photo diode array (PDA) detector.

    Techniques: High Performance Liquid Chromatography, Marker

    HBXIP enhances the glucose metabolism reprogramming of breast cancer cells through miR-183/96/182 in vitro A. The levels of lactate in the culture media of MCF-7 cells were measured by an Agilent 1100 series high-performance liquid chromatography (HPLC) system and normalized to cell number. B. The levels of intracellular glucose were detected by glucose-lactate biosense tester SBA-40E and normalized based on the protein concentration in MCF-7 cells. C. The levels of intracellular ROS were assessed by flow cytometry analysis in MCF-7 cells. D–F. The relative levels of lactate, intracellular glucose and ROS were measured using above methods in HBXIP-transfected MCF-7 cells treated with anti-miR-183 (anti-miR-96 or anti-miR-182). Statistically significant differences are indicated: * P

    Journal: Oncotarget

    Article Title: The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer

    doi:

    Figure Lengend Snippet: HBXIP enhances the glucose metabolism reprogramming of breast cancer cells through miR-183/96/182 in vitro A. The levels of lactate in the culture media of MCF-7 cells were measured by an Agilent 1100 series high-performance liquid chromatography (HPLC) system and normalized to cell number. B. The levels of intracellular glucose were detected by glucose-lactate biosense tester SBA-40E and normalized based on the protein concentration in MCF-7 cells. C. The levels of intracellular ROS were assessed by flow cytometry analysis in MCF-7 cells. D–F. The relative levels of lactate, intracellular glucose and ROS were measured using above methods in HBXIP-transfected MCF-7 cells treated with anti-miR-183 (anti-miR-96 or anti-miR-182). Statistically significant differences are indicated: * P

    Article Snippet: Lactate production detection Transfected MCF-7 and T47D cells were cultured for 24 h. The lactate levels in the culture media were determined by an Agilent 1100 series high-performance liquid chromatography (HPLC) system (Hewlett-Packard Corporation, USA) [ ].

    Techniques: In Vitro, High Performance Liquid Chromatography, Protein Concentration, Flow Cytometry, Cytometry, Transfection

    HBXIP regulates glucose metabolism reprogramming and downregulates SCO2 and PDHA1 in breast cancer A. The levels of lactate in the culture media of MCF-7 and T47D cells were measured by an Agilent 1100 series high-performance liquid chromatography (HPLC) system and normalized to cell number. B. The levels of intracellular glucose were detected by glucose-lactate biosense tester SBA-40E and normalized based on the protein concentration in MCF-7 and T47D cells. C. The levels of intracellular ROS were assessed by flow cytometry analysis in MCF-7 and T47D cells. D. The protein levels of HBXIP, PDHA1 and SCO2 were examined by Western blot analysis in MCF-7 and T47D cells. E. The expression levels of SCO2, PDHA1 and HBXIP protein were examined by IHC analysis in clinical breast cancer tissues using tissue microarrays, which were from the same tissue paraffin block. F. The percentage of staining gradations of SCO2, PDHA1 and HBXIP of tissue microarrays containing 95 cases of clinical breast cancer tissues was shown. Statistically significant differences are indicated: * P

    Journal: Oncotarget

    Article Title: The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer

    doi:

    Figure Lengend Snippet: HBXIP regulates glucose metabolism reprogramming and downregulates SCO2 and PDHA1 in breast cancer A. The levels of lactate in the culture media of MCF-7 and T47D cells were measured by an Agilent 1100 series high-performance liquid chromatography (HPLC) system and normalized to cell number. B. The levels of intracellular glucose were detected by glucose-lactate biosense tester SBA-40E and normalized based on the protein concentration in MCF-7 and T47D cells. C. The levels of intracellular ROS were assessed by flow cytometry analysis in MCF-7 and T47D cells. D. The protein levels of HBXIP, PDHA1 and SCO2 were examined by Western blot analysis in MCF-7 and T47D cells. E. The expression levels of SCO2, PDHA1 and HBXIP protein were examined by IHC analysis in clinical breast cancer tissues using tissue microarrays, which were from the same tissue paraffin block. F. The percentage of staining gradations of SCO2, PDHA1 and HBXIP of tissue microarrays containing 95 cases of clinical breast cancer tissues was shown. Statistically significant differences are indicated: * P

    Article Snippet: Lactate production detection Transfected MCF-7 and T47D cells were cultured for 24 h. The lactate levels in the culture media were determined by an Agilent 1100 series high-performance liquid chromatography (HPLC) system (Hewlett-Packard Corporation, USA) [ ].

    Techniques: High Performance Liquid Chromatography, Protein Concentration, Flow Cytometry, Cytometry, Western Blot, Expressing, Immunohistochemistry, Blocking Assay, Staining

    ( a ) Representative HPLC-MS chromatogram (HILIC-ESI-MS) from site U1357, 7.15 mbsf, showing the presence of major intact polar lipid isoprenoidal glycerol dialkyl glycerol tetraethers (IPL-GDGTs) and bacterial lipids in the mass range m/z 1200–1800. The HPLC-MS chromatogram is depicted as a density map showing retention time on the x axis, m/z on the y axis and the relative peak intensity by grey shading. ( b ) Structural variety in the head groups and biphytanyl moieties of the detected IPL-GDGTs. Note that during the ether cleavage reaction the hydroxyl group in the biphytane (bp) side chain becomes dehydrated and forms biphytene (bp0:1). Position of the hydroxyl group and double bond were not determined. 1G, monohexose; 2G, dihexose; PG, phosphatidyl glycerol; HPH, hexose-phoshohexose; cren, crenarchaeol.

    Journal: The ISME Journal

    Article Title: Acetoclastic Methanosaeta are dominant methanogens in organic-rich Antarctic marine sediments

    doi: 10.1038/ismej.2017.150

    Figure Lengend Snippet: ( a ) Representative HPLC-MS chromatogram (HILIC-ESI-MS) from site U1357, 7.15 mbsf, showing the presence of major intact polar lipid isoprenoidal glycerol dialkyl glycerol tetraethers (IPL-GDGTs) and bacterial lipids in the mass range m/z 1200–1800. The HPLC-MS chromatogram is depicted as a density map showing retention time on the x axis, m/z on the y axis and the relative peak intensity by grey shading. ( b ) Structural variety in the head groups and biphytanyl moieties of the detected IPL-GDGTs. Note that during the ether cleavage reaction the hydroxyl group in the biphytane (bp) side chain becomes dehydrated and forms biphytene (bp0:1). Position of the hydroxyl group and double bond were not determined. 1G, monohexose; 2G, dihexose; PG, phosphatidyl glycerol; HPH, hexose-phoshohexose; cren, crenarchaeol.

    Article Snippet: Separation of IPL-GDGTs was achieved by reverse phase chromatography using a Zorbax Eclipse XDB C18 column (5 μm, 10 × 250 mm, Agilent Technologies, Santa Clara, CA, USA) on an Agilent 1200 series high-performance liquid chromatography (HPLC) system equipped with an Agilent 1200 series fraction collector ( ).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Hydrophilic Interaction Liquid Chromatography

    FAE quantitative HPLC assay of free (free L.f. 5221) and microencapsulated L. fermentum NCIMB 5221 (APA L.f. 5221). Uninoculated MRS-EFA was used as a negative control. The presented data represents the amount of unhydrolysed EFA remaining in solution ( A ) and the amount of FA produced ( B ), as measured by HPLC peak area data. Each point represents the mean of triplicates and the error bars represent the standard deviations.These results demonstrate no significant difference in FA production between the free and encapsulated L. fermentum NCIMB 5221.

    Journal: Pharmaceuticals

    Article Title: Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization

    doi: 10.3390/ph5020236

    Figure Lengend Snippet: FAE quantitative HPLC assay of free (free L.f. 5221) and microencapsulated L. fermentum NCIMB 5221 (APA L.f. 5221). Uninoculated MRS-EFA was used as a negative control. The presented data represents the amount of unhydrolysed EFA remaining in solution ( A ) and the amount of FA produced ( B ), as measured by HPLC peak area data. Each point represents the mean of triplicates and the error bars represent the standard deviations.These results demonstrate no significant difference in FA production between the free and encapsulated L. fermentum NCIMB 5221.

    Article Snippet: De Man, Rogosa, Sharpe (MRS) broth and Methanol of high-performance liquid chromatography (HPLC) grade were obtained from Fisher Scientific (Ottawa, ON, Canada).

    Techniques: qHplc Assay, Negative Control, Produced, High Performance Liquid Chromatography