high-performance liquid chromatography Search Results


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  • 99
    Millipore performance liquid chromatography hplc
    Comparison of insulin B chain cleavage between <t>NEP,</t> NEP F563L , and NEP S546E . A. <t>HPLC</t> profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.
    Performance Liquid Chromatography Hplc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 348 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/performance liquid chromatography hplc/product/Millipore
    Average 99 stars, based on 348 article reviews
    Price from $9.99 to $1999.99
    performance liquid chromatography hplc - by Bioz Stars, 2020-08
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    99
    Millipore high performance liquid chromatography grade
    Comparison of insulin B chain cleavage between <t>NEP,</t> NEP F563L , and NEP S546E . A. <t>HPLC</t> profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.
    High Performance Liquid Chromatography Grade, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography grade/product/Millipore
    Average 99 stars, based on 87 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatography grade - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    94
    Millipore naoh
    Comparison of insulin B chain cleavage between <t>NEP,</t> NEP F563L , and NEP S546E . A. <t>HPLC</t> profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.
    Naoh, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 7059 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/naoh/product/Millipore
    Average 94 stars, based on 7059 article reviews
    Price from $9.99 to $1999.99
    naoh - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of insulin B chain cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.

    Journal: PLoS ONE

    Article Title: Active Site Mutations Change the Cleavage Specificity of Neprilysin

    doi: 10.1371/journal.pone.0032343

    Figure Lengend Snippet: Comparison of insulin B chain cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.

    Article Snippet: HPLC assays Cleavage of physiological peptides was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of the purified NEP or its mutants with 15 µM insulin B chain (Sigma Aldrich), 24 µM Aß1–40 (Anaspec), or 64 µM leu-ENK (Sigma) in 100 µL of 20 mM MES, pH 6.5, at 37°C.

    Techniques: High Performance Liquid Chromatography, Mutagenesis

    Comparison of Aß 1–40 cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC cleavage profile of Aß 1–40 cleavage by NEP and NEP mutants at ∼30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of Aß 1–40 from Table 2 . Reactions were carried out at 37°C with 15 µM Aß 1–40 in 20 mM MES, pH 6.5.

    Journal: PLoS ONE

    Article Title: Active Site Mutations Change the Cleavage Specificity of Neprilysin

    doi: 10.1371/journal.pone.0032343

    Figure Lengend Snippet: Comparison of Aß 1–40 cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC cleavage profile of Aß 1–40 cleavage by NEP and NEP mutants at ∼30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of Aß 1–40 from Table 2 . Reactions were carried out at 37°C with 15 µM Aß 1–40 in 20 mM MES, pH 6.5.

    Article Snippet: HPLC assays Cleavage of physiological peptides was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of the purified NEP or its mutants with 15 µM insulin B chain (Sigma Aldrich), 24 µM Aß1–40 (Anaspec), or 64 µM leu-ENK (Sigma) in 100 µL of 20 mM MES, pH 6.5, at 37°C.

    Techniques: High Performance Liquid Chromatography, Mutagenesis

    HPLC chromatographs of mCP. (a) mCP direct from vendor (Aldrich). Absorbances versus elution time were obtained using a broadband light source. The small inset panels show the UV–vis spectra (200 to 600 nm) of indicator components eluted at different times. (b) Purified mCP.

    Journal: Environmental Science & Technology

    Article Title: Purification and Characterization of meta-Cresol Purple for Spectrophotometric Seawater pH Measurements

    doi: 10.1021/es200665d

    Figure Lengend Snippet: HPLC chromatographs of mCP. (a) mCP direct from vendor (Aldrich). Absorbances versus elution time were obtained using a broadband light source. The small inset panels show the UV–vis spectra (200 to 600 nm) of indicator components eluted at different times. (b) Purified mCP.

    Article Snippet: Figure a shows an HPLC chromatograph of an unpurified Aldrich mCP sample on a Primesep B2 column.

    Techniques: High Performance Liquid Chromatography, Purification

    Metabolism profile of PBCHO by CYP6AA7/CPR and CYP9M10/CPR microsomes. ( A ) Control: PBCHO (10 µM) treated with CYP/CPR microsomes (100 pmol P450 in 0.1 M Tris pH = 7.5) for 15 min at 30 °C without NADPH. The black arrows indicate the peaks for PBCHO and PBCOOH; ( B ) PBCHO metabolized by CYP9M10/CPR microsomes. The red arrows indicate the PBCOOH and the reduced amount of PBCHO; ( C ) PBCHO metabolized by CYP6AA7/CPR microsomes. The red arrows indicate the PBCOOH and the reduced amount of PBCHO; ( D ) Metabolism rate of PBCHO with different CYP/CPR microsomes. Statistical significance is represented by P ≤ 0.05, with the letters using one-way ANOVA.

    Journal: Scientific Reports

    Article Title: The function of two P450s, CYP9M10 and CYP6AA7, in the permethrin resistance of Culex quinquefasciatus

    doi: 10.1038/s41598-017-00486-0

    Figure Lengend Snippet: Metabolism profile of PBCHO by CYP6AA7/CPR and CYP9M10/CPR microsomes. ( A ) Control: PBCHO (10 µM) treated with CYP/CPR microsomes (100 pmol P450 in 0.1 M Tris pH = 7.5) for 15 min at 30 °C without NADPH. The black arrows indicate the peaks for PBCHO and PBCOOH; ( B ) PBCHO metabolized by CYP9M10/CPR microsomes. The red arrows indicate the PBCOOH and the reduced amount of PBCHO; ( C ) PBCHO metabolized by CYP6AA7/CPR microsomes. The red arrows indicate the PBCOOH and the reduced amount of PBCHO; ( D ) Metabolism rate of PBCHO with different CYP/CPR microsomes. Statistical significance is represented by P ≤ 0.05, with the letters using one-way ANOVA.

    Article Snippet: HPLC analysis and permethrin, PBOH, and PBCHO metabolism study Permethrin, PBOH and PBCHO (HPLC grade, Sigma Aldrich) were initially dissolved in acetonitrile and PBCOOH (HPLC grade, Sigma Aldrich) in methanol to create 1 mM standard solutions.

    Techniques:

    Rg1 induced PPAR γ expression and inhibited by GW9662 in cerebral ischemic rats and in OGD rat cortical neurons. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Neuroprotective Effect of the Ginsenoside Rg1 on Cerebral Ischemic Injury In Vivo and In Vitro Is Mediated by PPARγ-Regulated Antioxidative and Anti-Inflammatory Pathways

    doi: 10.1155/2017/7842082

    Figure Lengend Snippet: Rg1 induced PPAR γ expression and inhibited by GW9662 in cerebral ischemic rats and in OGD rat cortical neurons. ∗∗ P

    Article Snippet: Antibodies and Reagents In this study, Rg1 (HPLC purity > 98%) and GW9662 (HPLC purity > 98%) were procured from Sigma-Aldrich (cat #68317, cat #M6191).

    Techniques: Expressing

    Effect of Rg1 on the protein expression of PPAR γ and NF- κ B 65 in brain tissue of rats. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Neuroprotective Effect of the Ginsenoside Rg1 on Cerebral Ischemic Injury In Vivo and In Vitro Is Mediated by PPARγ-Regulated Antioxidative and Anti-Inflammatory Pathways

    doi: 10.1155/2017/7842082

    Figure Lengend Snippet: Effect of Rg1 on the protein expression of PPAR γ and NF- κ B 65 in brain tissue of rats. ∗∗ P

    Article Snippet: Antibodies and Reagents In this study, Rg1 (HPLC purity > 98%) and GW9662 (HPLC purity > 98%) were procured from Sigma-Aldrich (cat #68317, cat #M6191).

    Techniques: Expressing

    Effect of Rg1 on the protein expression of PPAR γ and NF- κ B 65 in the cortical neurons of rats. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Neuroprotective Effect of the Ginsenoside Rg1 on Cerebral Ischemic Injury In Vivo and In Vitro Is Mediated by PPARγ-Regulated Antioxidative and Anti-Inflammatory Pathways

    doi: 10.1155/2017/7842082

    Figure Lengend Snippet: Effect of Rg1 on the protein expression of PPAR γ and NF- κ B 65 in the cortical neurons of rats. ∗∗ P

    Article Snippet: Antibodies and Reagents In this study, Rg1 (HPLC purity > 98%) and GW9662 (HPLC purity > 98%) were procured from Sigma-Aldrich (cat #68317, cat #M6191).

    Techniques: Expressing

    Migration across a fibronectin-coated plate is significantly limited upon combination treatment with SAHA and EGCG. (A) 2 × 10 3 MCF-7 cells were plated in a 96-well plate coated with fibronectin (FN). After 72 h of treatment with the indicated compounds the round stopper was removed from the center of each well, the wells were washed, and regular culture media was added. Cells were given 24 h to migrate before being fixed and stained with crystal violet. Cells that had migrated into the center region were counted and compared between the indicated treatments (n = 3). (B-D) As above, in the MDA-MB-157, MDA-MB-231, and HCC1806 TNBC cell lines (n=3). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Journal: Anticancer research

    Article Title: SAHA and EGCG Promote Apoptosis in Triple-negative Breast Cancer Cells, Possibly Through the Modulation of cIAP2

    doi: 10.21873/anticanres.13922

    Figure Lengend Snippet: Migration across a fibronectin-coated plate is significantly limited upon combination treatment with SAHA and EGCG. (A) 2 × 10 3 MCF-7 cells were plated in a 96-well plate coated with fibronectin (FN). After 72 h of treatment with the indicated compounds the round stopper was removed from the center of each well, the wells were washed, and regular culture media was added. Cells were given 24 h to migrate before being fixed and stained with crystal violet. Cells that had migrated into the center region were counted and compared between the indicated treatments (n = 3). (B-D) As above, in the MDA-MB-157, MDA-MB-231, and HCC1806 TNBC cell lines (n=3). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Article Snippet: EGCG (≥ 97% pure, HPLC) and SAHA (≥ 98% pure, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Migration, Staining, Multiple Displacement Amplification

    SAHA and EGCG significantly reduced the expression of cIAP2 in two TNBC cell lines. (A) qRT-PCR using cIAP2 primers was performed in MCF-7 cells after 3-day treatments with the indicated compounds (n=3). GAPDH was used for comparison; (B-D) As above, in MDA-MB-157, MDA-MB-231, and HCC1806 cells (n=3). EGCG alone was able to significantly reduce the expression of cIAP2 in MDA-MB-231 cells (C). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Journal: Anticancer research

    Article Title: SAHA and EGCG Promote Apoptosis in Triple-negative Breast Cancer Cells, Possibly Through the Modulation of cIAP2

    doi: 10.21873/anticanres.13922

    Figure Lengend Snippet: SAHA and EGCG significantly reduced the expression of cIAP2 in two TNBC cell lines. (A) qRT-PCR using cIAP2 primers was performed in MCF-7 cells after 3-day treatments with the indicated compounds (n=3). GAPDH was used for comparison; (B-D) As above, in MDA-MB-157, MDA-MB-231, and HCC1806 cells (n=3). EGCG alone was able to significantly reduce the expression of cIAP2 in MDA-MB-231 cells (C). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Article Snippet: EGCG (≥ 97% pure, HPLC) and SAHA (≥ 98% pure, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Expressing, Quantitative RT-PCR, Multiple Displacement Amplification

    SAHA alone significantly increased the expression of caspase 7 in all four breast cancer cell lines. (A) qRT-PCR using caspase 7 primers was performed for MCF-7 cells after 3-day treatments with the indicated compounds (n=3). GAPDH was used for comparison; (B-D) As above, in MDA-MB-157, MDA-MB-231, and HCC1806 cells (n=3). EGCG alone significantly reduced the expression of caspase 7 in MDA-MB-231 cells (C). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Journal: Anticancer research

    Article Title: SAHA and EGCG Promote Apoptosis in Triple-negative Breast Cancer Cells, Possibly Through the Modulation of cIAP2

    doi: 10.21873/anticanres.13922

    Figure Lengend Snippet: SAHA alone significantly increased the expression of caspase 7 in all four breast cancer cell lines. (A) qRT-PCR using caspase 7 primers was performed for MCF-7 cells after 3-day treatments with the indicated compounds (n=3). GAPDH was used for comparison; (B-D) As above, in MDA-MB-157, MDA-MB-231, and HCC1806 cells (n=3). EGCG alone significantly reduced the expression of caspase 7 in MDA-MB-231 cells (C). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Article Snippet: EGCG (≥ 97% pure, HPLC) and SAHA (≥ 98% pure, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Expressing, Quantitative RT-PCR, Multiple Displacement Amplification

    The combination of SAHA and EGCG significantly decreased the expression cIAP2 at the protein level in all four breast cancer cell lines. (A) Combination of SAHA and EGCG significantly reduced the expression of cIAP2 in MCF-7 cells after 72 h of treatment (n=3). (B-D) As above, in MDA-MB-157, MDA-MB-231, and HCC1806 TNBC cell lines (n=3). Protein levels were normalized to the control, and error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Journal: Anticancer research

    Article Title: SAHA and EGCG Promote Apoptosis in Triple-negative Breast Cancer Cells, Possibly Through the Modulation of cIAP2

    doi: 10.21873/anticanres.13922

    Figure Lengend Snippet: The combination of SAHA and EGCG significantly decreased the expression cIAP2 at the protein level in all four breast cancer cell lines. (A) Combination of SAHA and EGCG significantly reduced the expression of cIAP2 in MCF-7 cells after 72 h of treatment (n=3). (B-D) As above, in MDA-MB-157, MDA-MB-231, and HCC1806 TNBC cell lines (n=3). Protein levels were normalized to the control, and error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Article Snippet: EGCG (≥ 97% pure, HPLC) and SAHA (≥ 98% pure, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Expressing, Multiple Displacement Amplification

    HMT (H3K27me3) activity increased upon treatment with SAHA and EGCG in the ERα-positive MCF-7 cell line. (A) MCF-7 cells were treated for 72 h with the indicated compounds before nuclear extraction occurred. Nuclear extracts were then used in the HMT (H3K27me3) activity assay (n=3). (B-D) As above in MDA-MB-157, MDA-MB-231, and HCC1806 TNBC cell lines (n=3). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Journal: Anticancer research

    Article Title: SAHA and EGCG Promote Apoptosis in Triple-negative Breast Cancer Cells, Possibly Through the Modulation of cIAP2

    doi: 10.21873/anticanres.13922

    Figure Lengend Snippet: HMT (H3K27me3) activity increased upon treatment with SAHA and EGCG in the ERα-positive MCF-7 cell line. (A) MCF-7 cells were treated for 72 h with the indicated compounds before nuclear extraction occurred. Nuclear extracts were then used in the HMT (H3K27me3) activity assay (n=3). (B-D) As above in MDA-MB-157, MDA-MB-231, and HCC1806 TNBC cell lines (n=3). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Article Snippet: EGCG (≥ 97% pure, HPLC) and SAHA (≥ 98% pure, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: HMT Assay, Activity Assay, Multiple Displacement Amplification

    SAHA and EGCG in combination significantly increased apoptosis of breast cancer cells. (A) MCF-7 cells treated for 3-days with the indicated compounds and then stained with annexin V and propidium iodide (n=3). Flow cytometric analysis was conducted utilizing the UAB Flow Core. (B-D) As above in MDA-MB-157, MDA-MB-231, and HCC1806 cell lines (n=3). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Journal: Anticancer research

    Article Title: SAHA and EGCG Promote Apoptosis in Triple-negative Breast Cancer Cells, Possibly Through the Modulation of cIAP2

    doi: 10.21873/anticanres.13922

    Figure Lengend Snippet: SAHA and EGCG in combination significantly increased apoptosis of breast cancer cells. (A) MCF-7 cells treated for 3-days with the indicated compounds and then stained with annexin V and propidium iodide (n=3). Flow cytometric analysis was conducted utilizing the UAB Flow Core. (B-D) As above in MDA-MB-157, MDA-MB-231, and HCC1806 cell lines (n=3). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines; * p

    Article Snippet: EGCG (≥ 97% pure, HPLC) and SAHA (≥ 98% pure, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Staining, Multiple Displacement Amplification

    HDAC activity decreased in breast cancer cells upon treatment with SAHA and EGCG, though SAHA alone had the same effect in two of the 4 cell lines. (A) MCF-7 cells were treated for 72 h with the indicated compounds before nuclear extraction was performed. Prepared nuclear extracts were used to quantify the activity of HDAC enzymes (n=3). (B-D) As above in MDA-MB-157, MDA-MB-231, and HCC1806 TNBC cell lines (n=3). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines); * p

    Journal: Anticancer research

    Article Title: SAHA and EGCG Promote Apoptosis in Triple-negative Breast Cancer Cells, Possibly Through the Modulation of cIAP2

    doi: 10.21873/anticanres.13922

    Figure Lengend Snippet: HDAC activity decreased in breast cancer cells upon treatment with SAHA and EGCG, though SAHA alone had the same effect in two of the 4 cell lines. (A) MCF-7 cells were treated for 72 h with the indicated compounds before nuclear extraction was performed. Prepared nuclear extracts were used to quantify the activity of HDAC enzymes (n=3). (B-D) As above in MDA-MB-157, MDA-MB-231, and HCC1806 TNBC cell lines (n=3). Error bars represent standard error of the mean (SEM), and ANOVA with Tukey post-hoc tests were performed on all cell lines); * p

    Article Snippet: EGCG (≥ 97% pure, HPLC) and SAHA (≥ 98% pure, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Activity Assay, Multiple Displacement Amplification

    Chou-Talalay method combination indices were determined for the combination of SAHA and EGCG utilizing CompuSyn software. For CI values

    Journal: Anticancer research

    Article Title: SAHA and EGCG Promote Apoptosis in Triple-negative Breast Cancer Cells, Possibly Through the Modulation of cIAP2

    doi: 10.21873/anticanres.13922

    Figure Lengend Snippet: Chou-Talalay method combination indices were determined for the combination of SAHA and EGCG utilizing CompuSyn software. For CI values

    Article Snippet: EGCG (≥ 97% pure, HPLC) and SAHA (≥ 98% pure, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Software