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    Agilent technologies 2100bioanalyzer high sensitivity dna kit agilent technologies
    2100bioanalyzer High Sensitivity Dna Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies q32854 agilent bioanalyzer high sensitivity dna kit agilent technologies
    Q32854 Agilent Bioanalyzer High Sensitivity Dna Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies bioanalyzer high sensitivity dna kit agilent technologies
    Bioanalyzer High Sensitivity Dna Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies dna high sensitivity bioanalyzer kit agilent technologies cat
    Dna High Sensitivity Bioanalyzer Kit Agilent Technologies Cat, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies sensitivity dna kit
    Test case dataset samples ( a ) <t>Bioanalyzer</t> analysis to assess the quality of the starting material for Rep1, Rep2_bad and Rep2_good. Plots show the <t>DNA</t> size distribution after fragmentation. X-axis shows the fragment size in base pairs (bp) and y-axis the florescence intensity proportional to DNA abundance (FU = florescence unit). The target fragment length was about 200bp (vertical blue line), but “Rep2_bad” fragment size distribution is shifted to the left towards smaller sizes. ( b ) QC diagnostic plots based on EM and GM scores for the test case samples Rep1 (left column), Rep2_bad (centre column) and Rep2_good (right column). Cross correlation profiles (top rows), RSC score (red dashed line) plot against the reference distribution of RSC values (middle row) and fingerprint plots (bottom row) for the three test case samples. Overall, they do not consistently single out Rep2_bad as the real problematic sample.
    Sensitivity Dna Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Test case dataset samples ( a ) Bioanalyzer analysis to assess the quality of the starting material for Rep1, Rep2_bad and Rep2_good. Plots show the DNA size distribution after fragmentation. X-axis shows the fragment size in base pairs (bp) and y-axis the florescence intensity proportional to DNA abundance (FU = florescence unit). The target fragment length was about 200bp (vertical blue line), but “Rep2_bad” fragment size distribution is shifted to the left towards smaller sizes. ( b ) QC diagnostic plots based on EM and GM scores for the test case samples Rep1 (left column), Rep2_bad (centre column) and Rep2_good (right column). Cross correlation profiles (top rows), RSC score (red dashed line) plot against the reference distribution of RSC values (middle row) and fingerprint plots (bottom row) for the three test case samples. Overall, they do not consistently single out Rep2_bad as the real problematic sample.

    Journal: bioRxiv

    Article Title: A ChIC solution for ChIP-seq quality assessment

    doi: 10.1101/2020.05.19.103887

    Figure Lengend Snippet: Test case dataset samples ( a ) Bioanalyzer analysis to assess the quality of the starting material for Rep1, Rep2_bad and Rep2_good. Plots show the DNA size distribution after fragmentation. X-axis shows the fragment size in base pairs (bp) and y-axis the florescence intensity proportional to DNA abundance (FU = florescence unit). The target fragment length was about 200bp (vertical blue line), but “Rep2_bad” fragment size distribution is shifted to the left towards smaller sizes. ( b ) QC diagnostic plots based on EM and GM scores for the test case samples Rep1 (left column), Rep2_bad (centre column) and Rep2_good (right column). Cross correlation profiles (top rows), RSC score (red dashed line) plot against the reference distribution of RSC values (middle row) and fingerprint plots (bottom row) for the three test case samples. Overall, they do not consistently single out Rep2_bad as the real problematic sample.

    Article Snippet: Libraries were created using Biomek FX automatic liquid handler (Beckman Coulter), then qualitatively and quantitatively checked using Agilent High Sensitivity DNA Kit (Agilent Technologies, 5067-4627) on a Bioanalyzer 2100 (Agilent Technologies).

    Techniques: Diagnostic Assay

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Variant overlap between DNA from PBMCs vs. FFPE tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively

    Journal: NPJ Genomic Medicine

    Article Title: A robust targeted sequencing approach for low input and variable quality DNA from clinical samples

    doi: 10.1038/s41525-017-0041-4

    Figure Lengend Snippet: Variant overlap between DNA from PBMCs vs. FFPE tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively

    Article Snippet: To obtain an idea of the degree of fragmentation in FFPE-derived gDNA samples prior to sonication, HD200 and two clinical FFPE samples were analyzed by a High Sensitivity DNA analysis kit on a Bioanalyzer 2100 (Agilent, Santa Clara, CA) (Supplementary Figure ).

    Techniques: Variant Assay, Formalin-fixed Paraffin-Embedded

    Distribution of DNA methylation in different genomic contexts in non-infected and M. bovis -infected bovine alveolar macrophages (24 hpi). Analysis of WGBS data from M. bovis -infected and non-infected bovine alveolar macrophages (bAM) revealed that genomic methylation, in the context of CpGs, was not altered at any of the sequence features outlined (intergenic regions, gene bodies, or promoters with or without CpG islands (CGIs) in the host following infection. Blue and red violins represent non-infected and M. bovis -infected bAM, respectively.

    Journal: Scientific Reports

    Article Title: The bovine alveolar macrophage DNA methylome is resilient to infection with Mycobacterium bovis

    doi: 10.1038/s41598-018-37618-z

    Figure Lengend Snippet: Distribution of DNA methylation in different genomic contexts in non-infected and M. bovis -infected bovine alveolar macrophages (24 hpi). Analysis of WGBS data from M. bovis -infected and non-infected bovine alveolar macrophages (bAM) revealed that genomic methylation, in the context of CpGs, was not altered at any of the sequence features outlined (intergenic regions, gene bodies, or promoters with or without CpG islands (CGIs) in the host following infection. Blue and red violins represent non-infected and M. bovis -infected bAM, respectively.

    Article Snippet: Pyrosequencing Genomic DNA was extracted from M. bovis -infected and control bAM (isolated from a parallel set of four animals to those used for WGBS) and quantified with the High-Sensitivity DNA Assay Kit (Agilent Technologies).

    Techniques: DNA Methylation Assay, Infection, Methylation, Sequencing

    Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)

    doi: 10.1007/978-1-4939-7768-0_12

    Figure Lengend Snippet: Size distribution analysis of a NOMe-seq sample and library Shown is a Bioanalyzer trace, obtained using an Agilent 2100 Bioanalyzer instrument and an Agilent High Sensitivity DNA chip, of the DNA after M.CviPI treatment and fragmentation using a Covaris S220 sonicator (a) and of the resultant NOMe-seq library (b) . The leftmost and rightmost peaks (labeled 43 and 113) are size markers of 35 bp and 10380 bp, respectively. The average length of the fragmented DNA is calculated to be 150 bp whereas the average length of the library fragments is calculated to be 280 bp. (c) For comparison to the Bioanalyzer traces, the gel images of the fragmented DNA and the NOMe-seq library are also shown.

    Article Snippet: Covaris sonicator (S220, formerly S2) Covaris MicroTUBE AFA Pre-slit Snap-Cap 6x16mm Nanodrop Spectrophotometer DNA High Sensitivity Kit (Agilent) for use with Agilent 2100 Bioanalyzer

    Techniques: Chromatin Immunoprecipitation, Labeling