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  • 96
    Thermo Fisher qubit high sensitivity dna kit
    Factors which influence urinary cfDNA concentration. All <t>DNA</t> concentrations were determined using the <t>Qubit</t> HS double-stranded DNA kit. Figure A) the effect of tumour grade on urinary cfDNA, B) effect of stage, C) effect of tumour size, D) effect of the number of tumours.
    Qubit High Sensitivity Dna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    PerkinElmer dna high sensitivity reagent kit
    Factors which influence urinary cfDNA concentration. All <t>DNA</t> concentrations were determined using the <t>Qubit</t> HS double-stranded DNA kit. Figure A) the effect of tumour grade on urinary cfDNA, B) effect of stage, C) effect of tumour size, D) effect of the number of tumours.
    Dna High Sensitivity Reagent Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 96/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna high sensitivity reagent kit/product/PerkinElmer
    Average 96 stars, based on 196 article reviews
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    dna high sensitivity reagent kit - by Bioz Stars, 2020-05
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    99
    Agilent technologies high sensitivity dna kit
    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular <t>RNA</t> was extracted and subjected to RT-PCR, and the products were separated on a 2% <t>DNA</t> gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.
    High Sensitivity Dna Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 7169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity dna kit/product/Agilent technologies
    Average 99 stars, based on 7169 article reviews
    Price from $9.99 to $1999.99
    high sensitivity dna kit - by Bioz Stars, 2020-05
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    93
    Thermo Fisher double stranded dna high sensitivity kit
    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular <t>RNA</t> was extracted and subjected to RT-PCR, and the products were separated on a 2% <t>DNA</t> gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.
    Double Stranded Dna High Sensitivity Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/double stranded dna high sensitivity kit/product/Thermo Fisher
    Average 93 stars, based on 6 article reviews
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    double stranded dna high sensitivity kit - by Bioz Stars, 2020-05
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    92
    PerkinElmer caliper labchip gx dna high sensitivity reagent kit
    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular <t>RNA</t> was extracted and subjected to RT-PCR, and the products were separated on a 2% <t>DNA</t> gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.
    Caliper Labchip Gx Dna High Sensitivity Reagent Kit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caliper labchip gx dna high sensitivity reagent kit/product/PerkinElmer
    Average 92 stars, based on 47 article reviews
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    caliper labchip gx dna high sensitivity reagent kit - by Bioz Stars, 2020-05
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    93
    Roche high sensitivity dna kit
    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular <t>RNA</t> was extracted and subjected to RT-PCR, and the products were separated on a 2% <t>DNA</t> gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.
    High Sensitivity Dna Kit, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    high sensitivity dna kit - by Bioz Stars, 2020-05
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    94
    Agilent technologies high sensitivity dna analysis kit
    Variant overlap between <t>DNA</t> from PBMCs vs. <t>FFPE</t> tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively
    High Sensitivity Dna Analysis Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 606 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity dna analysis kit/product/Agilent technologies
    Average 94 stars, based on 606 article reviews
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    high sensitivity dna analysis kit - by Bioz Stars, 2020-05
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    93
    Agilent technologies high sensitivity dna analysis kits
    Variant overlap between <t>DNA</t> from PBMCs vs. <t>FFPE</t> tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively
    High Sensitivity Dna Analysis Kits, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity dna analysis kits/product/Agilent technologies
    Average 93 stars, based on 39 article reviews
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    high sensitivity dna analysis kits - by Bioz Stars, 2020-05
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    Image Search Results


    Factors which influence urinary cfDNA concentration. All DNA concentrations were determined using the Qubit HS double-stranded DNA kit. Figure A) the effect of tumour grade on urinary cfDNA, B) effect of stage, C) effect of tumour size, D) effect of the number of tumours.

    Journal: Bladder Cancer (Amsterdam, Netherlands)

    Article Title: Toward Personalised Liquid Biopsies for Urothelial Carcinoma: Characterisation of ddPCR and Urinary cfDNA for the Detection of the TERT 228 G > A/T Mutation

    doi: 10.3233/BLC-170152

    Figure Lengend Snippet: Factors which influence urinary cfDNA concentration. All DNA concentrations were determined using the Qubit HS double-stranded DNA kit. Figure A) the effect of tumour grade on urinary cfDNA, B) effect of stage, C) effect of tumour size, D) effect of the number of tumours.

    Article Snippet: DNA concentrations were determined using the Qubit high sensitivity DNA kit (Thermo, # ) and cfDNA size determined by Bioanalyzer using high sensitivity chips (Agilent, # 5067-4626). cfDNA preparations from 104 patients yielded > 10 ng DNA and were included for analysis.

    Techniques: Concentration Assay

    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular RNA was extracted and subjected to RT-PCR, and the products were separated on a 2% DNA gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Expanded subgenomic mRNA transcriptome and coding capacity of a nidovirus

    doi: 10.1073/pnas.1706696114

    Figure Lengend Snippet: Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular RNA was extracted and subjected to RT-PCR, and the products were separated on a 2% DNA gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.

    Article Snippet: The final dsDNA library was validated with an Agilent High Sensitivity DNA Kit (Agilent Technologies) and subjected to RNA-seq using Illumina HiSeq (paired-end reads, 100-bp read length) with one sample per lane.

    Techniques: Amplification, Clone Assay, Generated, Functional Assay, Sequencing, Infection, Reverse Transcription Polymerase Chain Reaction, Produced, Polymerase Chain Reaction, Plasmid Preparation, Nucleic Acid Electrophoresis

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Distribution of DNA methylation in different genomic contexts in non-infected and M. bovis -infected bovine alveolar macrophages (24 hpi). Analysis of WGBS data from M. bovis -infected and non-infected bovine alveolar macrophages (bAM) revealed that genomic methylation, in the context of CpGs, was not altered at any of the sequence features outlined (intergenic regions, gene bodies, or promoters with or without CpG islands (CGIs) in the host following infection. Blue and red violins represent non-infected and M. bovis -infected bAM, respectively.

    Journal: Scientific Reports

    Article Title: The bovine alveolar macrophage DNA methylome is resilient to infection with Mycobacterium bovis

    doi: 10.1038/s41598-018-37618-z

    Figure Lengend Snippet: Distribution of DNA methylation in different genomic contexts in non-infected and M. bovis -infected bovine alveolar macrophages (24 hpi). Analysis of WGBS data from M. bovis -infected and non-infected bovine alveolar macrophages (bAM) revealed that genomic methylation, in the context of CpGs, was not altered at any of the sequence features outlined (intergenic regions, gene bodies, or promoters with or without CpG islands (CGIs) in the host following infection. Blue and red violins represent non-infected and M. bovis -infected bAM, respectively.

    Article Snippet: Pyrosequencing Genomic DNA was extracted from M. bovis -infected and control bAM (isolated from a parallel set of four animals to those used for WGBS) and quantified with the High-Sensitivity DNA Assay Kit (Agilent Technologies).

    Techniques: DNA Methylation Assay, Infection, Methylation, Sequencing

    Variant overlap between DNA from PBMCs vs. FFPE tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively

    Journal: NPJ Genomic Medicine

    Article Title: A robust targeted sequencing approach for low input and variable quality DNA from clinical samples

    doi: 10.1038/s41525-017-0041-4

    Figure Lengend Snippet: Variant overlap between DNA from PBMCs vs. FFPE tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively

    Article Snippet: To obtain an idea of the degree of fragmentation in FFPE-derived gDNA samples prior to sonication, HD200 and two clinical FFPE samples were analyzed by a High Sensitivity DNA analysis kit on a Bioanalyzer 2100 (Agilent, Santa Clara, CA) (Supplementary Figure ).

    Techniques: Variant Assay, Formalin-fixed Paraffin-Embedded