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    Agilent technologies 2100bioanalyzer high sensitivity dna kit agilent technologies
    2100bioanalyzer High Sensitivity Dna Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies q32854 agilent bioanalyzer high sensitivity dna kit agilent technologies
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    Agilent technologies dna high sensitivity bioanalyzer kit agilent technologies cat
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    Agilent technologies sensitivity dna kit
    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular <t>RNA</t> was extracted and subjected to RT-PCR, and the products were separated on a 2% <t>DNA</t> gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.
    Sensitivity Dna Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high sensitivity dna
    High Sensitivity <t>DNA</t> <t>Bioanalyzer</t> assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.
    High Sensitivity Dna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high sensitivity dna tapestation
    High Sensitivity <t>DNA</t> <t>Bioanalyzer</t> assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.
    High Sensitivity Dna Tapestation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high sensitivity dna reagent
    High Sensitivity <t>DNA</t> <t>Bioanalyzer</t> assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.
    High Sensitivity Dna Reagent, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies bioanalysis dna high sensitivity
    High Sensitivity <t>DNA</t> <t>Bioanalyzer</t> assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.
    Bioanalysis Dna High Sensitivity, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies dna high sensitivity labchips
    High Sensitivity <t>DNA</t> <t>Bioanalyzer</t> assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.
    Dna High Sensitivity Labchips, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high sensitivity dna screentape
    High Sensitivity <t>DNA</t> <t>Bioanalyzer</t> assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.
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    Agilent technologies high sensitivity dna bioanalyzer
    High Sensitivity <t>DNA</t> <t>Bioanalyzer</t> assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.
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    Agilent technologies dna high sensitivity chip
    <t>DNA</t> methylation levels inversely correlate with expression levels of Esrp2 and Esrp2-as . ( a ) Upper : genomic organization of Esrp2 (dark blue) and Esrp2-as variants 1-4 (v1-4, light blue) and the CGI overlapping the Esrp2 TSS (green). Positions of EPITYPER Amplicons A1-A14 are indicated by horizontal black bars, covering the DMR (pink), the CGI, and CGI shores on both sides. Lower : <t>MCIp-seq</t> detection of methylated DNA fragments in tumors (red) and normal WT mammary glands (blue) of animals at 20 and 24 weeks of age. Each lane represents average reads obtained for three individual samples. ( b , c ) Heatmap of DNA methylation levels in tumor samples ( n =11) and normal mammary gland tissue ( n =9) ( b ), or of various murine cell lines ( c ), with each row representing one individual sample and each column one CpG unit comprising of 1 to 4 individual CpG sites. Methylation levels are depicted by a color-coded gradient from 0% (light yellow) to 100% methylation (blue). Gray squares indicate failed measurements. ( d , e ) Correlation between average methylation of amplicons A1-A14 and Esrp2/Esrp2-as expression levels normalized to three reference genes, calculated by Spearman’s rank correlation for tumor/normal tissues ( d ) and cell lines ( e ). * P
    Dna High Sensitivity Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 1057 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular RNA was extracted and subjected to RT-PCR, and the products were separated on a 2% DNA gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Expanded subgenomic mRNA transcriptome and coding capacity of a nidovirus

    doi: 10.1073/pnas.1706696114

    Figure Lengend Snippet: Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular RNA was extracted and subjected to RT-PCR, and the products were separated on a 2% DNA gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.

    Article Snippet: The final dsDNA library was validated with an Agilent High Sensitivity DNA Kit (Agilent Technologies) and subjected to RNA-seq using Illumina HiSeq (paired-end reads, 100-bp read length) with one sample per lane.

    Techniques: Amplification, Clone Assay, Generated, Functional Assay, Sequencing, Infection, Reverse Transcription Polymerase Chain Reaction, Produced, Polymerase Chain Reaction, Plasmid Preparation, Nucleic Acid Electrophoresis

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Variant overlap between DNA from PBMCs vs. FFPE tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively

    Journal: NPJ Genomic Medicine

    Article Title: A robust targeted sequencing approach for low input and variable quality DNA from clinical samples

    doi: 10.1038/s41525-017-0041-4

    Figure Lengend Snippet: Variant overlap between DNA from PBMCs vs. FFPE tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively

    Article Snippet: To obtain an idea of the degree of fragmentation in FFPE-derived gDNA samples prior to sonication, HD200 and two clinical FFPE samples were analyzed by a High Sensitivity DNA analysis kit on a Bioanalyzer 2100 (Agilent, Santa Clara, CA) (Supplementary Figure ).

    Techniques: Variant Assay, Formalin-fixed Paraffin-Embedded

    High Sensitivity DNA Bioanalyzer assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.

    Journal: PLoS ONE

    Article Title: Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

    doi: 10.1371/journal.pone.0107259

    Figure Lengend Snippet: High Sensitivity DNA Bioanalyzer assay as checkpoint for correct size selection during library preparation. All nine samples showed adaptor/RNA/adaptor-constructs in appropriate sizes. One electropherogram is shown as representative example. The lengths of adaptor-ligated constructs from all nine samples were reported as indicated in the column peak size [bp] . The initial peak at 35 bp and the final peak at 10.380 bp are marker peaks that are system inherent included in all runs.

    Article Snippet: A concluding High Sensitivity DNA Assay on the Bioanalyzer 2100 (Agilent Technologies, Germany) revealed the generation of cDNA libraries with adaptor-ligated constructs in the correct size, which signified the successful amplification of mature miRNAs and piRNAs ( ).

    Techniques: Selection, Construct, Marker

    DNA methylation levels inversely correlate with expression levels of Esrp2 and Esrp2-as . ( a ) Upper : genomic organization of Esrp2 (dark blue) and Esrp2-as variants 1-4 (v1-4, light blue) and the CGI overlapping the Esrp2 TSS (green). Positions of EPITYPER Amplicons A1-A14 are indicated by horizontal black bars, covering the DMR (pink), the CGI, and CGI shores on both sides. Lower : MCIp-seq detection of methylated DNA fragments in tumors (red) and normal WT mammary glands (blue) of animals at 20 and 24 weeks of age. Each lane represents average reads obtained for three individual samples. ( b , c ) Heatmap of DNA methylation levels in tumor samples ( n =11) and normal mammary gland tissue ( n =9) ( b ), or of various murine cell lines ( c ), with each row representing one individual sample and each column one CpG unit comprising of 1 to 4 individual CpG sites. Methylation levels are depicted by a color-coded gradient from 0% (light yellow) to 100% methylation (blue). Gray squares indicate failed measurements. ( d , e ) Correlation between average methylation of amplicons A1-A14 and Esrp2/Esrp2-as expression levels normalized to three reference genes, calculated by Spearman’s rank correlation for tumor/normal tissues ( d ) and cell lines ( e ). * P

    Journal: Oncogene

    Article Title: Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer

    doi: 10.1038/onc.2017.246

    Figure Lengend Snippet: DNA methylation levels inversely correlate with expression levels of Esrp2 and Esrp2-as . ( a ) Upper : genomic organization of Esrp2 (dark blue) and Esrp2-as variants 1-4 (v1-4, light blue) and the CGI overlapping the Esrp2 TSS (green). Positions of EPITYPER Amplicons A1-A14 are indicated by horizontal black bars, covering the DMR (pink), the CGI, and CGI shores on both sides. Lower : MCIp-seq detection of methylated DNA fragments in tumors (red) and normal WT mammary glands (blue) of animals at 20 and 24 weeks of age. Each lane represents average reads obtained for three individual samples. ( b , c ) Heatmap of DNA methylation levels in tumor samples ( n =11) and normal mammary gland tissue ( n =9) ( b ), or of various murine cell lines ( c ), with each row representing one individual sample and each column one CpG unit comprising of 1 to 4 individual CpG sites. Methylation levels are depicted by a color-coded gradient from 0% (light yellow) to 100% methylation (blue). Gray squares indicate failed measurements. ( d , e ) Correlation between average methylation of amplicons A1-A14 and Esrp2/Esrp2-as expression levels normalized to three reference genes, calculated by Spearman’s rank correlation for tumor/normal tissues ( d ) and cell lines ( e ). * P

    Article Snippet: Size distribution was confirmed on a DNA High sensitivity Chip (Agilent Bioanalyzer), before proceeding with MCIp reaction using a SX8G-V52 robot (Diagenode, Liège, Belgium) for automated processing.

    Techniques: DNA Methylation Assay, Expressing, Methylation