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  • 99
    New England Biolabs phusion high fidelity polymerase chain reaction pcr mastermix
    Phusion High Fidelity Polymerase Chain Reaction Pcr Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 10x high fidelity pcr buffer
    10x High Fidelity Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    High Fidelity Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 772 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    High Fidelity Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    High Fidelity Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim expand high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    Expand High Fidelity Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche expand high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    Expand High Fidelity Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 1x high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    1x High Fidelity Pcr Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim 1x expand high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    1x Expand High Fidelity Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    High Fidelity Pcr Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher x phusion high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    X Phusion High Fidelity Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high fidelity pcr reaction buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    High Fidelity Pcr Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs pcr
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 18844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche 1x taq expand high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    1x Taq Expand High Fidelity Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum pcr supermix high fidelity buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    Platinum Pcr Supermix High Fidelity Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad iproof high fidelity pcr buffer
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    Iproof High Fidelity Pcr Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 PCR for MKPV DNA using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).

    Journal: PLoS Pathogens

    Article Title: Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys

    doi: 10.1371/journal.ppat.1008262

    Figure Lengend Snippet: Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 PCR for MKPV DNA using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).

    Article Snippet: DNA was screened for DrPV-1 sequences using Platinum Taq DNA polymerase and high fidelity PCR buffer (Invitrogen), primers Chap-DRPv-fwd and Chap-DRPv-rev, an initial temperature of 94˚C for 0.5 min, and 35 cycles of 94˚C for 0.25 min, 57˚C for 0.5 min and 68˚C for 1 min. Products were resolved by agarose gel electrophoresis.

    Techniques: Staining, Formalin-fixed Paraffin-Embedded, Polymerase Chain Reaction, In Situ Hybridization, Infection, Mouse Assay

    Map of the MKPV genome. (A-B) Maps of the MKPV/MuCPV strains from Centenary Institute (CI, accession MH670587), Memorial Sloan Kettering Cancer Center (MSKCC, accession MH670588) and New York City basements (wild-NY, MF175078). “Bowties” indicate terminal repeats (TR). (A) Single nucleotide variations (SNV) between the CI, MSKCC and wild-NY accessions. Vertical lines—differences between accessions. Half height vertical lines—polymorphisms within an accession. ▼; 2 bp insertion in the CI strain. ▲; 1 bp insertion in a CI sub-strain. Dashed lines—missing extremities in MSKCC and wild-NY accessions, which consist of the exterior inverted repeats in the full-length CI sequence. (B-C) Alternative splicing allows production of the polypeptides p10, p15, NS1, NS2, NP and VP. Black, brown or blue shading indicate the relative reading frames of ORFs. p15, p10 and NP could theoretically be produced from multiple transcripts. Orange or red indicate peptides present in LC-MS/MS datasets PXD014938 (this paper) or PXD010540 [ 9 ], respectively. Exon or intron sequences flanking splice sites are shown in black or red text, respectively. (C) Quantitation of spliced MKPV reads in RNAseq data pooled from two MKPV-infected kidneys. Columns indicate splice site usage (left y-axis); heights of arcs (right y-axis) indicate the abundance of specific splice combinations. See S4 Table for more information. (D-E) Detection of spliced transcripts by RT-dependent PCR, using primers mapped in A-B. Input templates were MKPV-infected (D) kidney DNA or (E) DNAse/ExoI-treated kidney RNA, converted (+RT) or mock-converted (-RT) to cDNA. RT-PCR products corresponding to transcripts 1 to 4 are indicated by white numbers. (F) Mapping of transcription start and stop sites by RACE. See S1 Fig for RACE details. Major 5’ and 3’ RACE products, indicated by black arrows and corresponding to transcripts 2 to 4 or polyadenylation signals A and B, were gel-purified and Sanger sequenced. Other RACE products mentioned in the text are indicated by white arrows.

    Journal: PLoS Pathogens

    Article Title: Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys

    doi: 10.1371/journal.ppat.1008262

    Figure Lengend Snippet: Map of the MKPV genome. (A-B) Maps of the MKPV/MuCPV strains from Centenary Institute (CI, accession MH670587), Memorial Sloan Kettering Cancer Center (MSKCC, accession MH670588) and New York City basements (wild-NY, MF175078). “Bowties” indicate terminal repeats (TR). (A) Single nucleotide variations (SNV) between the CI, MSKCC and wild-NY accessions. Vertical lines—differences between accessions. Half height vertical lines—polymorphisms within an accession. ▼; 2 bp insertion in the CI strain. ▲; 1 bp insertion in a CI sub-strain. Dashed lines—missing extremities in MSKCC and wild-NY accessions, which consist of the exterior inverted repeats in the full-length CI sequence. (B-C) Alternative splicing allows production of the polypeptides p10, p15, NS1, NS2, NP and VP. Black, brown or blue shading indicate the relative reading frames of ORFs. p15, p10 and NP could theoretically be produced from multiple transcripts. Orange or red indicate peptides present in LC-MS/MS datasets PXD014938 (this paper) or PXD010540 [ 9 ], respectively. Exon or intron sequences flanking splice sites are shown in black or red text, respectively. (C) Quantitation of spliced MKPV reads in RNAseq data pooled from two MKPV-infected kidneys. Columns indicate splice site usage (left y-axis); heights of arcs (right y-axis) indicate the abundance of specific splice combinations. See S4 Table for more information. (D-E) Detection of spliced transcripts by RT-dependent PCR, using primers mapped in A-B. Input templates were MKPV-infected (D) kidney DNA or (E) DNAse/ExoI-treated kidney RNA, converted (+RT) or mock-converted (-RT) to cDNA. RT-PCR products corresponding to transcripts 1 to 4 are indicated by white numbers. (F) Mapping of transcription start and stop sites by RACE. See S1 Fig for RACE details. Major 5’ and 3’ RACE products, indicated by black arrows and corresponding to transcripts 2 to 4 or polyadenylation signals A and B, were gel-purified and Sanger sequenced. Other RACE products mentioned in the text are indicated by white arrows.

    Article Snippet: DNA was screened for DrPV-1 sequences using Platinum Taq DNA polymerase and high fidelity PCR buffer (Invitrogen), primers Chap-DRPv-fwd and Chap-DRPv-rev, an initial temperature of 94˚C for 0.5 min, and 35 cycles of 94˚C for 0.25 min, 57˚C for 0.5 min and 68˚C for 1 min. Products were resolved by agarose gel electrophoresis.

    Techniques: Sequencing, Produced, Liquid Chromatography with Mass Spectroscopy, Quantitation Assay, Infection, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Purification