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  • 90
    Thermo Fisher high capacity cdna reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 91179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high capacity complementary dna reverse transcript kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    High Capacity Complementary Dna Reverse Transcript Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high capacity complementery dna reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    High Capacity Complementery Dna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transcriptor high capacity cdna reverse transcription kits
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    Transcriptor High Capacity Cdna Reverse Transcription Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman high capacity cdna reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    Taqman High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman reverse transcription reagent kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    Taqman Reverse Transcription Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 794 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cdna reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi high capacity cdna reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    Abi High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher multiscribe high capacity cdna reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    Multiscribe High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman microrna reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    Taqman Microrna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 19256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher complementary deoxyrinuleic acid cdna reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    Complementary Deoxyrinuleic Acid Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high capacity cdna reverse transcriptional kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    High Capacity Cdna Reverse Transcriptional Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high capacity complementary dna cdna synthesis kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    High Capacity Complementary Dna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high capacity reverse transcription kit
    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total <t>RNA</t> (200 ng) of DC was converted into <t>cDNA</t> and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].
    High Capacity Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total RNA (200 ng) of DC was converted into cDNA and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].

    Journal: PLoS ONE

    Article Title: The Tyrphostin Agent AG490 Prevents and Reverses Type 1 Diabetes in NOD Mice

    doi: 10.1371/journal.pone.0036079

    Figure Lengend Snippet: AG490 modulates phenotype and function of DC. A - Dendritic cells were generated from bone marrow of NOD mice treated either with AG490 or DMSO as described in materials and methods and stained directly with CD11c, MHC II, CD80 and CD86. Data shown represents CD11c gated population of pool fresh purified immature CD11c of AG490 and DMSO treated mice. B- Histograms represent expression of CD11c, CD86, CD80 and CD62L on gated CD11c population of mice treated with AG490 or DMSO in vivo . C- Bone marrow cells were isolated from NOD mice treated with AG490/DMSO for 5 weeks and then differentiated into DC in vitro as described. Total RNA (200 ng) of DC was converted into cDNA and then expression of Tgf-β1 was measured by Real-Time TaqMan Gene expression assays (Applied Biosystems). Data represents the mean (fold change) of two real-time RT-PCR experiments and the bars represent standard deviation of the mean. Fold change of Tgf-β1expression is shown (n = 5 mice/group, * p = 0.02). D- BMDCs were generated from BALB/c mice treated in vitro with 20 µM AG490 or DMSO for 12 hrs and then co-cultured with constant numbers of CD4+CD25− T-cells (1×10 5 ) with soluble anti-CD3 (2.5 ng/ml) for 72 hrs. Incorporation of 3 H-thymidine was measured in the last 16 hr of cell culture. Experiment was performed in triplicate format and repeated at least twice. Data analysis was performed using the student t -test and p value≤0.05 considered significant. Bars represent deviation of the mean (* p = 0.002, **p = 0.0002; ***p = 0.001). [Figures A B are intended to be in color online and black and white in print].

    Article Snippet: To screen the Taqman Array Mouse Immune Response, 200 ng of total RNA was converted to cDNA using High Capacity cDNA Reverse Transcription kit (Applied Biosystems).

    Techniques: Generated, Mouse Assay, Staining, Purification, Expressing, In Vivo, Isolation, In Vitro, Quantitative RT-PCR, Standard Deviation, Cell Culture

    HFD-fed iRhom2 KO mice have increased thermogenesis and browning of the white adipose tissue. A-C Thermal images (A), and BAT (B) or body (C) temperature of iRhom2 KO and WT mice fed with SD and HFD for 19 weeks. One experiment with 8 mice per group. D RT-PCR analysis of UCP1, PGC1α, Cidea, PRDM16, and Cox8b expression in SD-fed iRhom2 KO and HFD-fed WT and iRhom2 KO mice BAT samples compared to WT SD-fed control samples. Two experiments with 3–4 replicates in each. E, G Representative photographs of eWAT (E) and sWAT (G) UCP1 immunohistochemistry derived from iRhom2 KO and WT mice fed with HFD for 20 weeks. Scale bar = 100 μm. F, H Graphics showing the UCP1 percentage of area stained in the eWAT (F) and sWAT (H) of the mice described above. One experiment with 8 mice per group (with 2 photographs analyzed per mouse). I RT-PCR analysis of UCP1 in brown adipocytes differentiated in vitro from the stromal vascular fraction of 4–5 pooled WT or iRhom2 KO mice. Three independent experiments. J RT-PCR analysis of UCP1 in immortalized WT brown preadipocytes transduced with empty vector or iRhom2-HA, differentiated in vitro and stimulated with norepinephrine for 6 h. Two independent experiments. K HA expression in differentiated immortalized WT brown preadipocytes transduced with retrovirus containing iRhom2 cDNA fused to C-terminal HA tag (iR2-HA). As a negative control, we used the same cells transduced with retrovirus containing the empty vector (EV), and as a loading control we measured p97 protein level. Two independent experiments. L Mitochondrial oxygen consumption rate (OCR) of brown adipocytes differentiated in vitro from the stromal vascular fraction of 4–5 pooled WT and iRhom2 KO mice and stimulated or not with norepinephrine. The results were normalized to the protein content. Two experiments with one or two independent samples per genotype, respectively. M Mitochondrial proton leak of the cells described in L normalized to the protein content. Error bars represent SEM; * represents p

    Journal: Molecular Metabolism

    Article Title: Deletion of iRhom2 protects against diet-induced obesity by increasing thermogenesis

    doi: 10.1016/j.molmet.2019.10.006

    Figure Lengend Snippet: HFD-fed iRhom2 KO mice have increased thermogenesis and browning of the white adipose tissue. A-C Thermal images (A), and BAT (B) or body (C) temperature of iRhom2 KO and WT mice fed with SD and HFD for 19 weeks. One experiment with 8 mice per group. D RT-PCR analysis of UCP1, PGC1α, Cidea, PRDM16, and Cox8b expression in SD-fed iRhom2 KO and HFD-fed WT and iRhom2 KO mice BAT samples compared to WT SD-fed control samples. Two experiments with 3–4 replicates in each. E, G Representative photographs of eWAT (E) and sWAT (G) UCP1 immunohistochemistry derived from iRhom2 KO and WT mice fed with HFD for 20 weeks. Scale bar = 100 μm. F, H Graphics showing the UCP1 percentage of area stained in the eWAT (F) and sWAT (H) of the mice described above. One experiment with 8 mice per group (with 2 photographs analyzed per mouse). I RT-PCR analysis of UCP1 in brown adipocytes differentiated in vitro from the stromal vascular fraction of 4–5 pooled WT or iRhom2 KO mice. Three independent experiments. J RT-PCR analysis of UCP1 in immortalized WT brown preadipocytes transduced with empty vector or iRhom2-HA, differentiated in vitro and stimulated with norepinephrine for 6 h. Two independent experiments. K HA expression in differentiated immortalized WT brown preadipocytes transduced with retrovirus containing iRhom2 cDNA fused to C-terminal HA tag (iR2-HA). As a negative control, we used the same cells transduced with retrovirus containing the empty vector (EV), and as a loading control we measured p97 protein level. Two independent experiments. L Mitochondrial oxygen consumption rate (OCR) of brown adipocytes differentiated in vitro from the stromal vascular fraction of 4–5 pooled WT and iRhom2 KO mice and stimulated or not with norepinephrine. The results were normalized to the protein content. Two experiments with one or two independent samples per genotype, respectively. M Mitochondrial proton leak of the cells described in L normalized to the protein content. Error bars represent SEM; * represents p

    Article Snippet: First-strand cDNA was synthesized from total RNA using the SuperScript® III First-Strand Synthesis SuperMix or the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific).

    Techniques: Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunohistochemistry, Derivative Assay, Staining, In Vitro, Transduction, Plasmid Preparation, Negative Control

    The FPN1B transcript was highly expressed in duodenum and bone marrow. (A) Total RNA of multiple tissues was probed with FPN1B or 1A specific probes in northern blots. (B) qRT-PCR was used to check the expression of FPN1B and FPN1A in cDNA of mouse tissues.

    Journal:

    Article Title: A ferroportin transcript that lacks an iron-responsive element enables duodenal and erythroid precursor cells to evade translational repression

    doi: 10.1016/j.cmet.2009.03.006

    Figure Lengend Snippet: The FPN1B transcript was highly expressed in duodenum and bone marrow. (A) Total RNA of multiple tissues was probed with FPN1B or 1A specific probes in northern blots. (B) qRT-PCR was used to check the expression of FPN1B and FPN1A in cDNA of mouse tissues.

    Article Snippet: The total RNA was prepared with TRIzol® reagent (Invitrogen); cDNA was prepared with High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Foster City, CA).

    Techniques: Northern Blot, Quantitative RT-PCR, Expressing

    Sen1 is required to maintain basal levels of RNR1 through transcription. A) Untreated WT, Sen1-1 and Sen1-2 cells were grown in liquid YPD to mid-log and used for making whole cell protein extracts by TCA precipitation. Samples were analyzed by western blotting with Rnr1, Rnr2, Sml1, Rap1 and Gapdh antibodies. CBBR stands for Coomassie Brilliant Blue R. B) Semi-quantitative analysis of RNR1, HUG1 genes; logarithmically grown Sen1 strains (WT, sen1-1, Sen1-2) in YPD were left untreated for 3 hr. Total RNA was extracted and reverse transcribed to cDNA. Semi-quantitative PCR analysis was performed to assess the levels of RNR1, HUG1 and ACT1 transcripts. The expected sizes of the PCR product for RNR1, HUG1 and ACT1 are 219, 190, and 520 bp respectively. Two repeats of RT-PCR amplifications are shown here.

    Journal: PLoS ONE

    Article Title: Sen1p Contributes to Genomic Integrity by Regulating Expression of Ribonucleotide Reductase 1 (RNR1) in Saccharomyces cerevisiae

    doi: 10.1371/journal.pone.0064798

    Figure Lengend Snippet: Sen1 is required to maintain basal levels of RNR1 through transcription. A) Untreated WT, Sen1-1 and Sen1-2 cells were grown in liquid YPD to mid-log and used for making whole cell protein extracts by TCA precipitation. Samples were analyzed by western blotting with Rnr1, Rnr2, Sml1, Rap1 and Gapdh antibodies. CBBR stands for Coomassie Brilliant Blue R. B) Semi-quantitative analysis of RNR1, HUG1 genes; logarithmically grown Sen1 strains (WT, sen1-1, Sen1-2) in YPD were left untreated for 3 hr. Total RNA was extracted and reverse transcribed to cDNA. Semi-quantitative PCR analysis was performed to assess the levels of RNR1, HUG1 and ACT1 transcripts. The expected sizes of the PCR product for RNR1, HUG1 and ACT1 are 219, 190, and 520 bp respectively. Two repeats of RT-PCR amplifications are shown here.

    Article Snippet: 2 µg of total RNA was reverse transcribed to synthesize cDNA using High Capacity RNA-to-cDNA™ Kit (Life Technologies Corporation, California) according to the manufacturer instructions.

    Techniques: TCA Precipitation, Western Blot, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    Functional studies of the FANCM :p.Arg658*, p.Gln1701* and p.Arg1931* truncating variants using the patient-derived FANCM − / − EGF280 cell line. a Western blot showing the FANCM expression in EGF280 cells complemented with lentiviral vectors harboring the three different variants. Bands corresponding to truncated FANCM protein were visible for EGF280 + p.Gln1701* and p.Arg1931*, and no bands were present for the EGF280 + p.Arg658*. b Study of the expression of the FANCM protein in EGF280 + p.Arg658*. The c.1972C > T base substitution, causing the p.Arg658* variant abrogates a digestion site for the restriction enzyme Tse I that is present in the wild-type (wt) cDNA sequence. Total RNA was extracted from EGF280 + wt FANCM and from the EGF280 + p.Arg658* and subjected to reverse transcription. PCR-amplified cDNA products were digested with Tse I. Digested and undigested cDNAs were loaded. In the first two lanes are shown bands of 386 bp corresponding to uncut wt cDNA, and bands of 257 and 129 bp corresponding to cut wt cDNA. In next two lanes bands of 386 bp indicate that p.Arg658* cDNA was not cut due to the c.1972C > T base substitution abrogating the Tse I site. In the two lanes after the molecular weight marker (M) undigested and digested products of the two previous PCR products were mixed 1:1 and loaded as a control. c Analysis of diepoxybutane (DEB) sensitivity on cell survival. The EGF280 cells expressing p.Arg658* are significantly more sensitive to DEB than the cells expressing p.Gln1701* or p.Arg1931* ( P-values from Tukey’s range test are reported in Supplementary Table 4 ). EGF280 and EGF280 + wtFANCM are used as controls ( N = 3; error bars: standard deviation). d Chromosome fragility induced by DEB treatment (100 ng/ml). Here, the chromatidic break patterns of the cells expressing wt FANCM, of the cells harboring p.Gln1701* or p.Arg1931* variants, and of the native EGF280 cells or the cells expressing p.Arg658* were statistically different. ( P-values from chi-squared test; N = 2). e Analysis of cellular sensitivity to olaparib. Contrarily to what we observed in the DEB sensitivity assays, survival rates of the different complemented cell lines were apparently not different. Human fibroblasts ( BRCA2 − / − ) were homozygous for the c.469 A > T (p.Lys157*) truncating variant and were used as a positive control. ( P-values from Tukey’s range test are reported in Supplementary Table 5 ; N = 3; error bars: standard deviation). All blots derive from the same experiment and were processed in parallel

    Journal: NPJ Breast Cancer

    Article Title: The FANCM:p.Arg658* truncating variant is associated with risk of triple-negative breast cancer

    doi: 10.1038/s41523-019-0127-5

    Figure Lengend Snippet: Functional studies of the FANCM :p.Arg658*, p.Gln1701* and p.Arg1931* truncating variants using the patient-derived FANCM − / − EGF280 cell line. a Western blot showing the FANCM expression in EGF280 cells complemented with lentiviral vectors harboring the three different variants. Bands corresponding to truncated FANCM protein were visible for EGF280 + p.Gln1701* and p.Arg1931*, and no bands were present for the EGF280 + p.Arg658*. b Study of the expression of the FANCM protein in EGF280 + p.Arg658*. The c.1972C > T base substitution, causing the p.Arg658* variant abrogates a digestion site for the restriction enzyme Tse I that is present in the wild-type (wt) cDNA sequence. Total RNA was extracted from EGF280 + wt FANCM and from the EGF280 + p.Arg658* and subjected to reverse transcription. PCR-amplified cDNA products were digested with Tse I. Digested and undigested cDNAs were loaded. In the first two lanes are shown bands of 386 bp corresponding to uncut wt cDNA, and bands of 257 and 129 bp corresponding to cut wt cDNA. In next two lanes bands of 386 bp indicate that p.Arg658* cDNA was not cut due to the c.1972C > T base substitution abrogating the Tse I site. In the two lanes after the molecular weight marker (M) undigested and digested products of the two previous PCR products were mixed 1:1 and loaded as a control. c Analysis of diepoxybutane (DEB) sensitivity on cell survival. The EGF280 cells expressing p.Arg658* are significantly more sensitive to DEB than the cells expressing p.Gln1701* or p.Arg1931* ( P-values from Tukey’s range test are reported in Supplementary Table 4 ). EGF280 and EGF280 + wtFANCM are used as controls ( N = 3; error bars: standard deviation). d Chromosome fragility induced by DEB treatment (100 ng/ml). Here, the chromatidic break patterns of the cells expressing wt FANCM, of the cells harboring p.Gln1701* or p.Arg1931* variants, and of the native EGF280 cells or the cells expressing p.Arg658* were statistically different. ( P-values from chi-squared test; N = 2). e Analysis of cellular sensitivity to olaparib. Contrarily to what we observed in the DEB sensitivity assays, survival rates of the different complemented cell lines were apparently not different. Human fibroblasts ( BRCA2 − / − ) were homozygous for the c.469 A > T (p.Lys157*) truncating variant and were used as a positive control. ( P-values from Tukey’s range test are reported in Supplementary Table 5 ; N = 3; error bars: standard deviation). All blots derive from the same experiment and were processed in parallel

    Article Snippet: Reverse transcription was performed using High-Capacity RNA-to-cDNA Kit (Thermofisher); a cDNA region corresponding to the FANCM sequence containing the amino acid (AA) position Arg658 was amplified by PCR using the forward: 5’-AGTAACAGGCAGGTCCTTCA-3´and reverse: 5’-TGATCTTGCCACAGTCTCCA-3’ primers.

    Techniques: Functional Assay, Derivative Assay, Western Blot, Expressing, Variant Assay, Sequencing, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker, Standard Deviation, Positive Control

    ED 50 analysis of different gene targets . Apolipoprotein B (ApoB) small interfering RNA (siRNA), TTR UsiRNA, and factor VII UsiRNA formulated in C 18:1 -norArg-C 16 :CHEMS:CHOL:DMPE-PEG2K liposomes. Dose-dependent decrease in liver ( a ) ApoB mRNA with ApoB siRNA, ( b ) TTR mRNA with TTR UsiRNA, and ( c ) serum factor VII protein activity with factor VII UsiRNA. Formulated siRNA was administered from 2 to 0.01 mg/kg, and quantitative reverse transcription (RT)-PCR analyses were performed at 48 hours postdose. Data are normalized to phosphate-buffered saline (PBS)-treated control mice as mean ± SE, n = 8 or 5/group. ( d ) siRNA-induced inhibition of ApoB mRNA in mouse liver using 5′RACE assay. Arrow depicting 250 bp RNA-induced silencing complex (RISC)-mediated cleavage product from 5′RACE-PCR using gene-specific primers GSP2 and anchor primer. Treatment groups include ApoB siRNA and a nonspecific control siRNA. First lane: 100 bp DNA ladder; second lane: ApoB siRNA-treated liver; third lane: control siRNA-treated liver.

    Journal: Molecular Therapy

    Article Title: An Amino Acid-based Amphoteric Liposomal Delivery System for Systemic Administration of siRNA

    doi: 10.1038/mt.2011.56

    Figure Lengend Snippet: ED 50 analysis of different gene targets . Apolipoprotein B (ApoB) small interfering RNA (siRNA), TTR UsiRNA, and factor VII UsiRNA formulated in C 18:1 -norArg-C 16 :CHEMS:CHOL:DMPE-PEG2K liposomes. Dose-dependent decrease in liver ( a ) ApoB mRNA with ApoB siRNA, ( b ) TTR mRNA with TTR UsiRNA, and ( c ) serum factor VII protein activity with factor VII UsiRNA. Formulated siRNA was administered from 2 to 0.01 mg/kg, and quantitative reverse transcription (RT)-PCR analyses were performed at 48 hours postdose. Data are normalized to phosphate-buffered saline (PBS)-treated control mice as mean ± SE, n = 8 or 5/group. ( d ) siRNA-induced inhibition of ApoB mRNA in mouse liver using 5′RACE assay. Arrow depicting 250 bp RNA-induced silencing complex (RISC)-mediated cleavage product from 5′RACE-PCR using gene-specific primers GSP2 and anchor primer. Treatment groups include ApoB siRNA and a nonspecific control siRNA. First lane: 100 bp DNA ladder; second lane: ApoB siRNA-treated liver; third lane: control siRNA-treated liver.

    Article Snippet: Total RNA (50–100 ng) was reverse transcribed into complementary DNA using Applied Biosystems High Capacity complementary DNA Archive Kit according to the manufacturer's protocol (Life Technologies, Carlsbad, CA).

    Techniques: Small Interfering RNA, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Mouse Assay, Inhibition, Polymerase Chain Reaction

    The complementary DNA (cDNA) sequence of the junction of exon 12–13 in normal individuals and junction 12–14 in a Wilson Disease patient, where exon 13 has been entirely skipped.

    Journal: Sultan Qaboos University Medical Journal

    Article Title: A Novel Splice-site Allelic Variant is Responsible for Wilson Disease in an Omani Family

    doi:

    Figure Lengend Snippet: The complementary DNA (cDNA) sequence of the junction of exon 12–13 in normal individuals and junction 12–14 in a Wilson Disease patient, where exon 13 has been entirely skipped.

    Article Snippet: Reverse transcription of DNA was performed using the high capacity complementary DNA (cDNA) Archive kit (Applied Biosystems, USA).

    Techniques: Sequencing

    Transcriptional analysis of DPV gC gene . Total RNA was isolated from the cells at each time point and converted to cDNA. Samples of cDNA (1 μl) were amplified using real-time quantitative PCR and SYBR green detection. Presented is the fold change in the expression of gC gene.

    Journal: Virology Journal

    Article Title: Identification and characterization of duck plague virus glycoprotein C gene and gene product

    doi: 10.1186/1743-422X-7-349

    Figure Lengend Snippet: Transcriptional analysis of DPV gC gene . Total RNA was isolated from the cells at each time point and converted to cDNA. Samples of cDNA (1 μl) were amplified using real-time quantitative PCR and SYBR green detection. Presented is the fold change in the expression of gC gene.

    Article Snippet: The amplification was performed in a 20 μl reaction mixture containing 9 μl of POWER High-Capacity cDNA Reverse Transcription Kits SYBR Green PCR master mix (Applied Biosystems), 0.5 μl of each primer, 1 μl of cDNA template and 9 μl of sterile ultra pure water.

    Techniques: Isolation, Amplification, Real-time Polymerase Chain Reaction, SYBR Green Assay, Expressing