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  • 99
    Millipore hi di formamide
    Hi Di Formamide, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hi di formamide/product/Millipore
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    hi di formamide - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher hi di formamide
    T4DP treatment in the absence of dNTPs. 10 µL of H4 DNA mixture containing 50 fmol of FAM-labeled DNA was incubated without ( a – c ) or with ( d , e ) SAP for 30 min at 37 °C. Then, 0.5 units of T4DP were added. The reactions were conducted in the absence of dNTPs. After the indicated time intervals, a portion of the reaction mixture was sampled to <t>HiDi-LIZ500</t> supplemented with 201 bp FAM-labeled DNAs. Data from samples taken immediately after ( a and d ), 5 min ( b and e ), and 20 min ( c ) after T4DP addition are shown. All data were y-axis scaled so that FAM peak areas of the 201 bp DNA (right most peak) are apparently even across the panels. Two calibrator peaks from the <t>LIZ</t> size standard (50 and 200 nucleotides) are indicated by filled triangles. The relevant size marker locations are also indicated. Peaks observed at a marker size of about 50 nt and 75 nt represent the degradation product of the FAM-labeled strand, which may be two nucleotides plus FAM (5’-FAM-AA-3’, indicated by asterisks (**)) and one nucleotide plus FAM (5’-FAM-A-3’, indicated by astersisk (*)), respectively. Electric charge of the FAM moiety might have pronounced effects on the mobility of DNA fragment as the nucleotide length decreases. The slight height increase of some peaks in ( c ) might be due to injection profile change due to prolonged incubation
    Hi Di Formamide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 8694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hi di formamide/product/Thermo Fisher
    Average 92 stars, based on 8694 article reviews
    Price from $9.99 to $1999.99
    hi di formamide - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    84
    Thermo Fisher hi di formamide ce ivd
    T4DP treatment in the absence of dNTPs. 10 µL of H4 DNA mixture containing 50 fmol of FAM-labeled DNA was incubated without ( a – c ) or with ( d , e ) SAP for 30 min at 37 °C. Then, 0.5 units of T4DP were added. The reactions were conducted in the absence of dNTPs. After the indicated time intervals, a portion of the reaction mixture was sampled to <t>HiDi-LIZ500</t> supplemented with 201 bp FAM-labeled DNAs. Data from samples taken immediately after ( a and d ), 5 min ( b and e ), and 20 min ( c ) after T4DP addition are shown. All data were y-axis scaled so that FAM peak areas of the 201 bp DNA (right most peak) are apparently even across the panels. Two calibrator peaks from the <t>LIZ</t> size standard (50 and 200 nucleotides) are indicated by filled triangles. The relevant size marker locations are also indicated. Peaks observed at a marker size of about 50 nt and 75 nt represent the degradation product of the FAM-labeled strand, which may be two nucleotides plus FAM (5’-FAM-AA-3’, indicated by asterisks (**)) and one nucleotide plus FAM (5’-FAM-A-3’, indicated by astersisk (*)), respectively. Electric charge of the FAM moiety might have pronounced effects on the mobility of DNA fragment as the nucleotide length decreases. The slight height increase of some peaks in ( c ) might be due to injection profile change due to prolonged incubation
    Hi Di Formamide Ce Ivd, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hi di formamide ce ivd/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hi di formamide ce ivd - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

    92
    Promega hi di formamide
    T4DP treatment in the absence of dNTPs. 10 µL of H4 DNA mixture containing 50 fmol of FAM-labeled DNA was incubated without ( a – c ) or with ( d , e ) SAP for 30 min at 37 °C. Then, 0.5 units of T4DP were added. The reactions were conducted in the absence of dNTPs. After the indicated time intervals, a portion of the reaction mixture was sampled to <t>HiDi-LIZ500</t> supplemented with 201 bp FAM-labeled DNAs. Data from samples taken immediately after ( a and d ), 5 min ( b and e ), and 20 min ( c ) after T4DP addition are shown. All data were y-axis scaled so that FAM peak areas of the 201 bp DNA (right most peak) are apparently even across the panels. Two calibrator peaks from the <t>LIZ</t> size standard (50 and 200 nucleotides) are indicated by filled triangles. The relevant size marker locations are also indicated. Peaks observed at a marker size of about 50 nt and 75 nt represent the degradation product of the FAM-labeled strand, which may be two nucleotides plus FAM (5’-FAM-AA-3’, indicated by asterisks (**)) and one nucleotide plus FAM (5’-FAM-A-3’, indicated by astersisk (*)), respectively. Electric charge of the FAM moiety might have pronounced effects on the mobility of DNA fragment as the nucleotide length decreases. The slight height increase of some peaks in ( c ) might be due to injection profile change due to prolonged incubation
    Hi Di Formamide, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hi di formamide/product/Promega
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    hi di formamide - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    T4DP treatment in the absence of dNTPs. 10 µL of H4 DNA mixture containing 50 fmol of FAM-labeled DNA was incubated without ( a – c ) or with ( d , e ) SAP for 30 min at 37 °C. Then, 0.5 units of T4DP were added. The reactions were conducted in the absence of dNTPs. After the indicated time intervals, a portion of the reaction mixture was sampled to HiDi-LIZ500 supplemented with 201 bp FAM-labeled DNAs. Data from samples taken immediately after ( a and d ), 5 min ( b and e ), and 20 min ( c ) after T4DP addition are shown. All data were y-axis scaled so that FAM peak areas of the 201 bp DNA (right most peak) are apparently even across the panels. Two calibrator peaks from the LIZ size standard (50 and 200 nucleotides) are indicated by filled triangles. The relevant size marker locations are also indicated. Peaks observed at a marker size of about 50 nt and 75 nt represent the degradation product of the FAM-labeled strand, which may be two nucleotides plus FAM (5’-FAM-AA-3’, indicated by asterisks (**)) and one nucleotide plus FAM (5’-FAM-A-3’, indicated by astersisk (*)), respectively. Electric charge of the FAM moiety might have pronounced effects on the mobility of DNA fragment as the nucleotide length decreases. The slight height increase of some peaks in ( c ) might be due to injection profile change due to prolonged incubation

    Journal: Communications Biology

    Article Title: Properties and efficient scrap-and-build repairing of mechanically sheared 3’ DNA ends

    doi: 10.1038/s42003-019-0660-7

    Figure Lengend Snippet: T4DP treatment in the absence of dNTPs. 10 µL of H4 DNA mixture containing 50 fmol of FAM-labeled DNA was incubated without ( a – c ) or with ( d , e ) SAP for 30 min at 37 °C. Then, 0.5 units of T4DP were added. The reactions were conducted in the absence of dNTPs. After the indicated time intervals, a portion of the reaction mixture was sampled to HiDi-LIZ500 supplemented with 201 bp FAM-labeled DNAs. Data from samples taken immediately after ( a and d ), 5 min ( b and e ), and 20 min ( c ) after T4DP addition are shown. All data were y-axis scaled so that FAM peak areas of the 201 bp DNA (right most peak) are apparently even across the panels. Two calibrator peaks from the LIZ size standard (50 and 200 nucleotides) are indicated by filled triangles. The relevant size marker locations are also indicated. Peaks observed at a marker size of about 50 nt and 75 nt represent the degradation product of the FAM-labeled strand, which may be two nucleotides plus FAM (5’-FAM-AA-3’, indicated by asterisks (**)) and one nucleotide plus FAM (5’-FAM-A-3’, indicated by astersisk (*)), respectively. Electric charge of the FAM moiety might have pronounced effects on the mobility of DNA fragment as the nucleotide length decreases. The slight height increase of some peaks in ( c ) might be due to injection profile change due to prolonged incubation

    Article Snippet: Fragment analysis Two microliters of GeneScan™ 500 LIZ® size standard was added to 1 mL of HiDi-formamide (Thermo Fisher Scientific, US-MA) to prepare HiDiLIZ500.

    Techniques: Labeling, Incubation, Marker, Injection

    Analysis of MEDEs using a capillary sequencer. FAM-labeled DNA samples were analyzed using a capillary sequencer after mixing with LIZ500 size standard-supplemented HiDi-formamide (HiDi-LIZ500). Two peaks of 100 and 150 nucleotides from the LIZ size standard, used to calibrate the electropherogram, are indicated by filled triangles. a H4 fraction DNA mixture before reaction. b Merged view of ten results obtained by analyzing H4 fraction DNA mixture independently. c – g Data obtained after different enzymatic treatments indicated on the left. Samples were purified using a DNA clean-up column before mixing with HiDi-LIZ500. T4DP, T4 DNA polymerase; SAP shrimp alkaline phosphatase; E3T4, combined treatments with exonuclease III and T4DP; SAP-T4DP, SAP-treated sample purified and treated with T4DP; E3T4-T4DP, E3T4-treated sample purified and treated with T4DP. In panel g , each peak is labeled with a corresponding base. All data were y-axis scaled so that sums of FAM peak areas are apparently even across the panels

    Journal: Communications Biology

    Article Title: Properties and efficient scrap-and-build repairing of mechanically sheared 3’ DNA ends

    doi: 10.1038/s42003-019-0660-7

    Figure Lengend Snippet: Analysis of MEDEs using a capillary sequencer. FAM-labeled DNA samples were analyzed using a capillary sequencer after mixing with LIZ500 size standard-supplemented HiDi-formamide (HiDi-LIZ500). Two peaks of 100 and 150 nucleotides from the LIZ size standard, used to calibrate the electropherogram, are indicated by filled triangles. a H4 fraction DNA mixture before reaction. b Merged view of ten results obtained by analyzing H4 fraction DNA mixture independently. c – g Data obtained after different enzymatic treatments indicated on the left. Samples were purified using a DNA clean-up column before mixing with HiDi-LIZ500. T4DP, T4 DNA polymerase; SAP shrimp alkaline phosphatase; E3T4, combined treatments with exonuclease III and T4DP; SAP-T4DP, SAP-treated sample purified and treated with T4DP; E3T4-T4DP, E3T4-treated sample purified and treated with T4DP. In panel g , each peak is labeled with a corresponding base. All data were y-axis scaled so that sums of FAM peak areas are apparently even across the panels

    Article Snippet: Fragment analysis Two microliters of GeneScan™ 500 LIZ® size standard was added to 1 mL of HiDi-formamide (Thermo Fisher Scientific, US-MA) to prepare HiDiLIZ500.

    Techniques: Labeling, Purification

    Exonuclease III treatment of MEDEs resulting in the complete removal of MSDEs. Exonuclease III was added to an H4 DNA mixture (10 nM) at a final concentration of 4 unit/μL. b – d At each indicated time point, a fraction (0.5 μL) of the reaction mixture was sampled to HiDi-LIZ500 and analyzed. Two calibrator peaks from the LIZ size standard (75 and 150 nucleotides) are indicated by filled triangles. The relevant size marker locations are indicated. Panel a shows identical data to that shown in Fig. 1a

    Journal: Communications Biology

    Article Title: Properties and efficient scrap-and-build repairing of mechanically sheared 3’ DNA ends

    doi: 10.1038/s42003-019-0660-7

    Figure Lengend Snippet: Exonuclease III treatment of MEDEs resulting in the complete removal of MSDEs. Exonuclease III was added to an H4 DNA mixture (10 nM) at a final concentration of 4 unit/μL. b – d At each indicated time point, a fraction (0.5 μL) of the reaction mixture was sampled to HiDi-LIZ500 and analyzed. Two calibrator peaks from the LIZ size standard (75 and 150 nucleotides) are indicated by filled triangles. The relevant size marker locations are indicated. Panel a shows identical data to that shown in Fig. 1a

    Article Snippet: Fragment analysis Two microliters of GeneScan™ 500 LIZ® size standard was added to 1 mL of HiDi-formamide (Thermo Fisher Scientific, US-MA) to prepare HiDiLIZ500.

    Techniques: Concentration Assay, Marker

    Terminal deoxynucleotidyl transferase (TdT) treatment of samples pretreated with different enzymes. H4 DNA mixture containing 25 fmol of FAM-labeled DNA was subjected to different enzymatic treatments, followed by TdT treatment. The panels show the samples before treatment ( a , identical to that shown in Fig. 1a ); samples treated with TdT alone ( b ); samples treated with SAP followed by TdT ( c ); samples treated serially with SAP, T4DP, and TdT ( d ); samples treated serially with a combination of exonuclease III and T4DP, T4DP alone, and TdT ( e ). Between the two enzymatic treatments, the samples were purified using the DNA clean-up column. Following the TdT treatment, the samples were purified using a DNA clean-up column before mixing with HiDi-LIZ500. All data were y-axis scaled so that sums of FAM peak areas are apparently even across the panels. Two calibrator peaks from the LIZ size standard (100 and 300 nucleotides) are indicated by filled triangles, and relevant size marker locations are also indicated

    Journal: Communications Biology

    Article Title: Properties and efficient scrap-and-build repairing of mechanically sheared 3’ DNA ends

    doi: 10.1038/s42003-019-0660-7

    Figure Lengend Snippet: Terminal deoxynucleotidyl transferase (TdT) treatment of samples pretreated with different enzymes. H4 DNA mixture containing 25 fmol of FAM-labeled DNA was subjected to different enzymatic treatments, followed by TdT treatment. The panels show the samples before treatment ( a , identical to that shown in Fig. 1a ); samples treated with TdT alone ( b ); samples treated with SAP followed by TdT ( c ); samples treated serially with SAP, T4DP, and TdT ( d ); samples treated serially with a combination of exonuclease III and T4DP, T4DP alone, and TdT ( e ). Between the two enzymatic treatments, the samples were purified using the DNA clean-up column. Following the TdT treatment, the samples were purified using a DNA clean-up column before mixing with HiDi-LIZ500. All data were y-axis scaled so that sums of FAM peak areas are apparently even across the panels. Two calibrator peaks from the LIZ size standard (100 and 300 nucleotides) are indicated by filled triangles, and relevant size marker locations are also indicated

    Article Snippet: Fragment analysis Two microliters of GeneScan™ 500 LIZ® size standard was added to 1 mL of HiDi-formamide (Thermo Fisher Scientific, US-MA) to prepare HiDiLIZ500.

    Techniques: Labeling, Purification, Marker