Journal: PLoS ONE

Article Title: Dysplastic Nodules with Glypican-3 Positive Immunostaining: A Risk for Early Hepatocellular Carcinoma

doi: 10.1371/journal.pone.0087120

Figure Lengend Snippet: Clonality analysis revealed that one band disappeared when DN was digested with Hha I, but two bands remained when the surrounding liver tissue was digested with Hha I. N, the surrounding liver tissue; N01-N03, 3 GPC3-positive staining DN; −, without Hha I digestion; +, with Hha I digestion.

Article Snippet: The genomic DNA extracted from DN and the surrounding normal liver tissues for the AR gene 10 µL each, was incubated with 5 U of Hha I (Promega, Madison, WI, USA) at 37°C for 3 h in a volume of 20 µL containing 2 µL of 10× reaction buffer.

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: CpG Site-Specific Regulation of Metallothionein-1 Gene Expression

doi: 10.3390/ijms21175946

Figure Lengend Snippet: Effect of partial CpG methylation in the MT1 promoter on Zn- and Cd-induced Lucia luciferase activity. MEFs were transfected with unmethylated or M.SssI- or HhaI methyltransferase–methylated Lucia reporter vectors carrying wild-type ( A ), point mutations of CpG8 ( B ) and CpG15 ( C ). Reporter gene expression was measured 6 h after Zn or Cd addition. Values are expressed as the means ± SD from three independent experiments. * p

Article Snippet: Lucia reporter vectors were methylated with CpG methyltransferase (M.SssI; CG→5m CG; New England Biolabs, Ipswich, MA, USA) or HhaI methyltransferase (GCGC→G5mCGC; New England Biolabs) for 1 h at 37 °C ( ).

Techniques: CpG Methylation Assay, Luciferase, Activity Assay, Transfection, Methylation, Expressing

Journal: International Journal of Molecular Sciences

Article Title: CpG Site-Specific Regulation of Metallothionein-1 Gene Expression

doi: 10.3390/ijms21175946

Figure Lengend Snippet: Effect of CpG methylation on MT1 promoter activity in MTF1 KO cells. ( A ) Cells were transfected with unmethylated Lucia reporter vectors. Reporter gene expression was measured 6 h after Zn or Cd addition. Values for neither treatment group were significantly different from the untreated group. ( B ) MTF1-KO cells were transfected with unmethylated or M.SssI- or HhaI methyltransferase-methylated Lucia reporter vectors. Values are expressed as the means ± SD from three independent experiments. * p

Article Snippet: Lucia reporter vectors were methylated with CpG methyltransferase (M.SssI; CG→5m CG; New England Biolabs, Ipswich, MA, USA) or HhaI methyltransferase (GCGC→G5mCGC; New England Biolabs) for 1 h at 37 °C ( ).

Techniques: CpG Methylation Assay, Activity Assay, Transfection, Expressing, Methylation