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  • 92
    New England Biolabs hha
    DNA methylation at the Slc17a6 promoter. a Schematic representation of the Slc17a6 gene. Solid boxes represent the 5′ and 3′UTRs and open boxes represent exons. A region spanning −255 to +90 bp relative to the transcriptional start site (TSS) is drawn below. The region (BS1, −144 to −20 bp) and <t>CpG</t> sites ( vertical arrows , CpGs 1–9) examined by clonal bisulphite sequencing are indicated, as is the region used in in vitro reporter assays (CpGs 1–10, −144 bp to +68 bp). Four different CpG methylation patterns were generated for reporter assays using three bacterial methyltransferases (MTs), M.SssI MT, <t>Hha</t> I MT and Hpa II MT either alone or in combination. Filled and open circles represent methylated and unmethylated CpGs, respectively. b DNA methylation in the adult hippocampus (P87 and 120). Each line of nine CpGs represents the DNA methylation state of one allele in one cell. Clones from controls (total n = 42 from 16 mice) as well as basal (total n = 33 from 16 mice) and high (total n = 34 from 17 mice) Slc17a6 expressors in the ethanol-exposed (EtOH) group are shown. A graph of % DNA methylation per clone is also shown. c The impact of Slc17a6 promoter methylation (CpGs 1–10, −144 to +68 bp) on luciferase activity in vitro. The results of three independent experiments in CAD cells are shown. Data are presented as mean and standard deviation. ** P
    Hha, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore restriction enzyme hha i
    DNA methylation at the Slc17a6 promoter. a Schematic representation of the Slc17a6 gene. Solid boxes represent the 5′ and 3′UTRs and open boxes represent exons. A region spanning −255 to +90 bp relative to the transcriptional start site (TSS) is drawn below. The region (BS1, −144 to −20 bp) and <t>CpG</t> sites ( vertical arrows , CpGs 1–9) examined by clonal bisulphite sequencing are indicated, as is the region used in in vitro reporter assays (CpGs 1–10, −144 bp to +68 bp). Four different CpG methylation patterns were generated for reporter assays using three bacterial methyltransferases (MTs), M.SssI MT, <t>Hha</t> I MT and Hpa II MT either alone or in combination. Filled and open circles represent methylated and unmethylated CpGs, respectively. b DNA methylation in the adult hippocampus (P87 and 120). Each line of nine CpGs represents the DNA methylation state of one allele in one cell. Clones from controls (total n = 42 from 16 mice) as well as basal (total n = 33 from 16 mice) and high (total n = 34 from 17 mice) Slc17a6 expressors in the ethanol-exposed (EtOH) group are shown. A graph of % DNA methylation per clone is also shown. c The impact of Slc17a6 promoter methylation (CpGs 1–10, −144 to +68 bp) on luciferase activity in vitro. The results of three independent experiments in CAD cells are shown. Data are presented as mean and standard deviation. ** P
    Restriction Enzyme Hha I, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa hha i
    T-RFLP profiles for the rhizomes of Alpinia officinarum Hance plants from various growth sites. The profiles were generated via either <t>Hha</t> I or <t>Msp</t> I mono-digestion T-RFLP method targeting amplified fungal rDNA ITS sequences from the rhizomes of A. officinarum grown at eight different sites at varying geographic distances from each other. The T-RFLP analysis was repeated in three independent samples for each site, and representative T-RFLP profiles are shown.
    Hha I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Sangon Biotech hha i
    T-RFLP profiles for the rhizomes of Alpinia officinarum Hance plants from various growth sites. The profiles were generated via either <t>Hha</t> I or <t>Msp</t> I mono-digestion T-RFLP method targeting amplified fungal rDNA ITS sequences from the rhizomes of A. officinarum grown at eight different sites at varying geographic distances from each other. The T-RFLP analysis was repeated in three independent samples for each site, and representative T-RFLP profiles are shown.
    Hha I, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher hha i
    Constrained ordination diagram for sample plots (deforested and forest soil samples) in the first two redundancy analysis (RDA) axes based on the soil chemical characteristics of the different sampling sites and their relationship with the verrucomicrobial T-RFLP generated by restriction with enzymes <t>Alu</t> I, Msp I and Hha I. Symbols refer to individual replicates ( A , B , C , D and E ) of the following sampling sites: open squares forest site located at Area 1 (FS1), open diamonds forest site located at Area 2 (FS2), open circle forest site located at Area 3 (FS3), black squares deforested site located at Area 1 (DS1), black diamonds deforested site located at Area 2 (DS2), black circle deforested site located at Area 3 (DS3)
    Hha I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    GE Healthcare hha i
    Nucleotide sequence of the 1,143-bp amplicon from the 23S rRNA gene from an H. pylori -resistant isolate. The arrow indicates a single point mutation, that is, a <t>T2717C</t> transition. The underline indicates the <t>Hha</t> I restriction site position. Arrows from the 5′ to 3′ termini indicate primer sequences.
    Hha I, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Boehringer Mannheim hha i
    Nucleotide sequence of the 1,143-bp amplicon from the 23S rRNA gene from an H. pylori -resistant isolate. The arrow indicates a single point mutation, that is, a <t>T2717C</t> transition. The underline indicates the <t>Hha</t> I restriction site position. Arrows from the 5′ to 3′ termini indicate primer sequences.
    Hha I, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher enzyme hha i
    Nucleotide sequence of the 1,143-bp amplicon from the 23S rRNA gene from an H. pylori -resistant isolate. The arrow indicates a single point mutation, that is, a <t>T2717C</t> transition. The underline indicates the <t>Hha</t> I restriction site position. Arrows from the 5′ to 3′ termini indicate primer sequences.
    Enzyme Hha I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher endonucleases hha i
    Nucleotide sequence of the 1,143-bp amplicon from the 23S rRNA gene from an H. pylori -resistant isolate. The arrow indicates a single point mutation, that is, a <t>T2717C</t> transition. The underline indicates the <t>Hha</t> I restriction site position. Arrows from the 5′ to 3′ termini indicate primer sequences.
    Endonucleases Hha I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega restriction enzyme hha i
    DNA- and mRNA-based T-RFLP profiles of nifH homologs from the hindgut of four termite species. PCR products (∼470 bp) were digested with <t>Hha</t> I. Peak assignment is based on T-RF lengths of the nifH homologs obtained from the respective clone
    Restriction Enzyme Hha I, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher restriction enzyme hha i
    Genotyping of the FABP2 (rs1799883) Ala54Thr polymorphism. After digestion of the polymerase chain reaction <t>PCR</t> product with <t>Hha</t> I in a polyacrylamide-gel electrophoresis stained with silver nitrate, P1 samples heterozygotes have three bands, at 180 bp, 99 bp and 81pb, P2 homozygotes have two bands at 99 bp and 81 bp. P3 homozygotes have one band at 180 bp.
    Restriction Enzyme Hha I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega hha i
    T-RFLP profile of the 16S rRNA genes of the actinobacterial population amplified from the roots of 6-week-old wheat grown in the field soil obtained from Swedes Flat and digested with Hin fI (a), <t>Hha</t> I (b), and Mbo I (c).
    Hha I, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher fastdigest hha i restriction enzyme
    T-RFLP profile of the 16S rRNA genes of the actinobacterial population amplified from the roots of 6-week-old wheat grown in the field soil obtained from Swedes Flat and digested with Hin fI (a), <t>Hha</t> I (b), and Mbo I (c).
    Fastdigest Hha I Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega hha i restriction endonuclease
    T-RFLP profile of the 16S rRNA genes of the actinobacterial population amplified from the roots of 6-week-old wheat grown in the field soil obtained from Swedes Flat and digested with Hin fI (a), <t>Hha</t> I (b), and Mbo I (c).
    Hha I Restriction Endonuclease, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Valiant restriction endonucleases hha i
    T-RFLP profile of the 16S rRNA genes of the actinobacterial population amplified from the roots of 6-week-old wheat grown in the field soil obtained from Swedes Flat and digested with Hin fI (a), <t>Hha</t> I (b), and Mbo I (c).
    Restriction Endonucleases Hha I, supplied by Valiant, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    DNA methylation at the Slc17a6 promoter. a Schematic representation of the Slc17a6 gene. Solid boxes represent the 5′ and 3′UTRs and open boxes represent exons. A region spanning −255 to +90 bp relative to the transcriptional start site (TSS) is drawn below. The region (BS1, −144 to −20 bp) and CpG sites ( vertical arrows , CpGs 1–9) examined by clonal bisulphite sequencing are indicated, as is the region used in in vitro reporter assays (CpGs 1–10, −144 bp to +68 bp). Four different CpG methylation patterns were generated for reporter assays using three bacterial methyltransferases (MTs), M.SssI MT, Hha I MT and Hpa II MT either alone or in combination. Filled and open circles represent methylated and unmethylated CpGs, respectively. b DNA methylation in the adult hippocampus (P87 and 120). Each line of nine CpGs represents the DNA methylation state of one allele in one cell. Clones from controls (total n = 42 from 16 mice) as well as basal (total n = 33 from 16 mice) and high (total n = 34 from 17 mice) Slc17a6 expressors in the ethanol-exposed (EtOH) group are shown. A graph of % DNA methylation per clone is also shown. c The impact of Slc17a6 promoter methylation (CpGs 1–10, −144 to +68 bp) on luciferase activity in vitro. The results of three independent experiments in CAD cells are shown. Data are presented as mean and standard deviation. ** P

    Journal: Epigenetics & Chromatin

    Article Title: Prenatal ethanol exposure alters adult hippocampal VGLUT2 expression with concomitant changes in promoter DNA methylation, H3K4 trimethylation and miR-467b-5p levels

    doi: 10.1186/s13072-015-0032-6

    Figure Lengend Snippet: DNA methylation at the Slc17a6 promoter. a Schematic representation of the Slc17a6 gene. Solid boxes represent the 5′ and 3′UTRs and open boxes represent exons. A region spanning −255 to +90 bp relative to the transcriptional start site (TSS) is drawn below. The region (BS1, −144 to −20 bp) and CpG sites ( vertical arrows , CpGs 1–9) examined by clonal bisulphite sequencing are indicated, as is the region used in in vitro reporter assays (CpGs 1–10, −144 bp to +68 bp). Four different CpG methylation patterns were generated for reporter assays using three bacterial methyltransferases (MTs), M.SssI MT, Hha I MT and Hpa II MT either alone or in combination. Filled and open circles represent methylated and unmethylated CpGs, respectively. b DNA methylation in the adult hippocampus (P87 and 120). Each line of nine CpGs represents the DNA methylation state of one allele in one cell. Clones from controls (total n = 42 from 16 mice) as well as basal (total n = 33 from 16 mice) and high (total n = 34 from 17 mice) Slc17a6 expressors in the ethanol-exposed (EtOH) group are shown. A graph of % DNA methylation per clone is also shown. c The impact of Slc17a6 promoter methylation (CpGs 1–10, −144 to +68 bp) on luciferase activity in vitro. The results of three independent experiments in CAD cells are shown. Data are presented as mean and standard deviation. ** P

    Article Snippet: A CpG methyltransferase (M.SssI ), Hpa II methyltransferase or Hha I methyltransferase (New England Biolabs, USA) were used either alone or in combination to methylate the promoter-containing construct and vector only according to the manufacturer’s instructions. pGL4.14-based plasmids (45 ng) and pGL4.74[hRluc /TK] (5 ng) were transiently transfected into CAD cells using Lipofectamine 2000 (Life Technologies, USA) according to the manufacturer’s protocol.

    Techniques: DNA Methylation Assay, Bisulfite Sequencing, In Vitro, CpG Methylation Assay, Generated, Methylation, Clone Assay, Mouse Assay, Luciferase, Activity Assay, Standard Deviation

    Protection of DNA against HsoI and HhaI cleavage by HhaI methyltransferase. Plasmid DNAs in lanes 1 to 3 were protected by in vitro methylation with HhaI methyltransferase. Plasmid DNAs in lanes 4 to 6 were not methylated. The plasmids in lanes 1 and

    Journal: Applied and Environmental Microbiology

    Article Title: Generation and Molecular Characterization of New Temperature-Sensitive Plasmids Intended for Genetic Engineering of Pasteurellaceae

    doi: 10.1128/AEM.71.11.7187-7195.2005

    Figure Lengend Snippet: Protection of DNA against HsoI and HhaI cleavage by HhaI methyltransferase. Plasmid DNAs in lanes 1 to 3 were protected by in vitro methylation with HhaI methyltransferase. Plasmid DNAs in lanes 4 to 6 were not methylated. The plasmids in lanes 1 and

    Article Snippet: Introduction of plasmid DNA into H. somnus was increased by approximately 4 orders of magnitude by in vitro methylation with HhaI methyltransferase, although the efficiency of transformation dropped as the size of the plasmid increased.

    Techniques: Plasmid Preparation, In Vitro, Methylation

    T-RFLP profiles for the rhizomes of Alpinia officinarum Hance plants from various growth sites. The profiles were generated via either Hha I or Msp I mono-digestion T-RFLP method targeting amplified fungal rDNA ITS sequences from the rhizomes of A. officinarum grown at eight different sites at varying geographic distances from each other. The T-RFLP analysis was repeated in three independent samples for each site, and representative T-RFLP profiles are shown.

    Journal: PLoS ONE

    Article Title: Fungal Endophytes of Alpinia officinarum Rhizomes: Insights on Diversity and Variation across Growth Years, Growth Sites, and the Inner Active Chemical Concentration

    doi: 10.1371/journal.pone.0115289

    Figure Lengend Snippet: T-RFLP profiles for the rhizomes of Alpinia officinarum Hance plants from various growth sites. The profiles were generated via either Hha I or Msp I mono-digestion T-RFLP method targeting amplified fungal rDNA ITS sequences from the rhizomes of A. officinarum grown at eight different sites at varying geographic distances from each other. The T-RFLP analysis was repeated in three independent samples for each site, and representative T-RFLP profiles are shown.

    Article Snippet: Restriction enzyme digestion was carried out in a total volume of 10 µl, containing 100 ng purified PCR product and 10 U Msp I or Hha I (Takara, Dalian, China) at 37°C for 3 h, followed by an inactivation step for 20 min at 65°C.

    Techniques: Generated, Amplification

    T-RFLP profiles for the rhizomes of Alpinia officinarum Hance plants at various ages. The profiles were produced via either Hha I or Msp I mono-digestion T-RFLP method targeting amplified fungal rDNA ITS sequences from the rhizomes of 1- to 4-year-old A. officinarum Hance planted at site LT. The T-RFLP analysis was repeated in three independent samples for each year, and the representative T-RFLP profiles are shown.

    Journal: PLoS ONE

    Article Title: Fungal Endophytes of Alpinia officinarum Rhizomes: Insights on Diversity and Variation across Growth Years, Growth Sites, and the Inner Active Chemical Concentration

    doi: 10.1371/journal.pone.0115289

    Figure Lengend Snippet: T-RFLP profiles for the rhizomes of Alpinia officinarum Hance plants at various ages. The profiles were produced via either Hha I or Msp I mono-digestion T-RFLP method targeting amplified fungal rDNA ITS sequences from the rhizomes of 1- to 4-year-old A. officinarum Hance planted at site LT. The T-RFLP analysis was repeated in three independent samples for each year, and the representative T-RFLP profiles are shown.

    Article Snippet: Restriction enzyme digestion was carried out in a total volume of 10 µl, containing 100 ng purified PCR product and 10 U Msp I or Hha I (Takara, Dalian, China) at 37°C for 3 h, followed by an inactivation step for 20 min at 65°C.

    Techniques: Produced, Amplification

    RFLP pattern of the ITS region under the restriction enzymes ( Hha I and Hinf I). Restriction with Hha I produced two fragments of 550 bp and 430 bp in Hybrid genotype and A. simplex s. s.. Hinf I produced four fragments of 620 bp, 370 bp, 300 bp, and 250 bp in Hybrid genotype, whereas produced two fragments of 620 bp and 250 bp in A. simplex s. s.. Lanes: 1, Hybrid genotype; 2, A. simplex s. s.; L, 100-bp ladder.

    Journal: PLoS ONE

    Article Title: Molecular Genotyping of Anisakis Larvae in Middle Eastern Japan and Endoscopic Evidence for Preferential Penetration of Normal over Atrophic Mucosa

    doi: 10.1371/journal.pone.0089188

    Figure Lengend Snippet: RFLP pattern of the ITS region under the restriction enzymes ( Hha I and Hinf I). Restriction with Hha I produced two fragments of 550 bp and 430 bp in Hybrid genotype and A. simplex s. s.. Hinf I produced four fragments of 620 bp, 370 bp, 300 bp, and 250 bp in Hybrid genotype, whereas produced two fragments of 620 bp and 250 bp in A. simplex s. s.. Lanes: 1, Hybrid genotype; 2, A. simplex s. s.; L, 100-bp ladder.

    Article Snippet: Each PCR product was digested by two restriction enzyme Hha I and Hinf I (Takara, Japan) and evaluated based on previous reports – , – .

    Techniques: Produced

    T-RFLP Analysis With HhaI and Clustering Analysis A, T-RFLP patterns of 16S rDNAs from stools digested with HhaI . The 16S rDNAs were extracted from samples and amplified with the universal primers 27F and 1492R. Each peak represents a terminal restriction fragment of a specific length that corresponds to a bacterial phylotype. The meconium sample numbers were 4, 5, 25, and 29. Out of 30 samples, the T-RFLP patterns of 26 samples were similar. B, Hierarchical clustering analysis of microbiota of meconium based on T-RFLP pattern derived from HhaI and MspI digestion. The figure shows the phylogenetic relationships among 30 meconium samples. The x-axis shows the % of similarity and 1 - 30, indicates the sample number.

    Journal: Iranian Journal of Pediatrics

    Article Title: The Early Intestinal Microbiota of Healthy Korean Newborns

    doi: 10.5812/ijp.2079

    Figure Lengend Snippet: T-RFLP Analysis With HhaI and Clustering Analysis A, T-RFLP patterns of 16S rDNAs from stools digested with HhaI . The 16S rDNAs were extracted from samples and amplified with the universal primers 27F and 1492R. Each peak represents a terminal restriction fragment of a specific length that corresponds to a bacterial phylotype. The meconium sample numbers were 4, 5, 25, and 29. Out of 30 samples, the T-RFLP patterns of 26 samples were similar. B, Hierarchical clustering analysis of microbiota of meconium based on T-RFLP pattern derived from HhaI and MspI digestion. The figure shows the phylogenetic relationships among 30 meconium samples. The x-axis shows the % of similarity and 1 - 30, indicates the sample number.

    Article Snippet: Restriction enzymes, HhaI (TaKara, 2,000 U) and MspI (TaKara, 3,000 U), were used to cut the purified PCR product.

    Techniques: Amplification, Derivative Assay

    PCR-RFLP patterns of the 26S rDNA PCR digested with Hha 1 (A) and Bst F51 (B) of the 11 standard strains of Malassezia . Lanes: M means molecular marker, 1: M. furfur (KCTC 7743), 2: M. sympodialis (KCTC 7985), 3: M. globosa (CBS 7966), 4: M. restricta (KCTC

    Journal: Annals of Dermatology

    Article Title: Molecular Analysis of Malassezia Microflora on the Skin of the Patients with Atopic Dermatitis

    doi: 10.5021/ad.2010.22.1.41

    Figure Lengend Snippet: PCR-RFLP patterns of the 26S rDNA PCR digested with Hha 1 (A) and Bst F51 (B) of the 11 standard strains of Malassezia . Lanes: M means molecular marker, 1: M. furfur (KCTC 7743), 2: M. sympodialis (KCTC 7985), 3: M. globosa (CBS 7966), 4: M. restricta (KCTC

    Article Snippet: Two restriction enzymes, Hha 1 (Takara Biomedicals, Otsu, Japan) and Bst F51 (SibEnzyme, Novosibirsk, Russia), were used to perform the 26S rDNA-RFLP of Malassezia .

    Techniques: Polymerase Chain Reaction, Marker

    Constrained ordination diagram for sample plots (deforested and forest soil samples) in the first two redundancy analysis (RDA) axes based on the soil chemical characteristics of the different sampling sites and their relationship with the verrucomicrobial T-RFLP generated by restriction with enzymes Alu I, Msp I and Hha I. Symbols refer to individual replicates ( A , B , C , D and E ) of the following sampling sites: open squares forest site located at Area 1 (FS1), open diamonds forest site located at Area 2 (FS2), open circle forest site located at Area 3 (FS3), black squares deforested site located at Area 1 (DS1), black diamonds deforested site located at Area 2 (DS2), black circle deforested site located at Area 3 (DS3)

    Journal: Antonie Van Leeuwenhoek

    Article Title: Verrucomicrobial community structure and abundance as indicators for changes in chemical factors linked to soil fertility

    doi: 10.1007/s10482-015-0530-3

    Figure Lengend Snippet: Constrained ordination diagram for sample plots (deforested and forest soil samples) in the first two redundancy analysis (RDA) axes based on the soil chemical characteristics of the different sampling sites and their relationship with the verrucomicrobial T-RFLP generated by restriction with enzymes Alu I, Msp I and Hha I. Symbols refer to individual replicates ( A , B , C , D and E ) of the following sampling sites: open squares forest site located at Area 1 (FS1), open diamonds forest site located at Area 2 (FS2), open circle forest site located at Area 3 (FS3), black squares deforested site located at Area 1 (DS1), black diamonds deforested site located at Area 2 (DS2), black circle deforested site located at Area 3 (DS3)

    Article Snippet: Purified products were split into three tubes (175 ng in each tube) and digested in separate 15 μl-reactions with 10 U of the restriction enzymes Alu I, Msp I and Hha I (Invitrogen, Carlsbad, CA, USA) for 3 h at 37 °C.

    Techniques: Sampling, Generated

    Constrained ordination diagram for sample plots (sugarcane rhizosphere soil samples collected on optimal and deficient soil fertility for sugarcane) in the first two redundancy analysis (RDA) axes based on the soil chemical characteristics of the different soil treatments and their relationship with the verrucomicrobial T-RFLP data from restriction profiles generated by enzymes Alu I, Msp I and Hha I

    Journal: Antonie Van Leeuwenhoek

    Article Title: Verrucomicrobial community structure and abundance as indicators for changes in chemical factors linked to soil fertility

    doi: 10.1007/s10482-015-0530-3

    Figure Lengend Snippet: Constrained ordination diagram for sample plots (sugarcane rhizosphere soil samples collected on optimal and deficient soil fertility for sugarcane) in the first two redundancy analysis (RDA) axes based on the soil chemical characteristics of the different soil treatments and their relationship with the verrucomicrobial T-RFLP data from restriction profiles generated by enzymes Alu I, Msp I and Hha I

    Article Snippet: Purified products were split into three tubes (175 ng in each tube) and digested in separate 15 μl-reactions with 10 U of the restriction enzymes Alu I, Msp I and Hha I (Invitrogen, Carlsbad, CA, USA) for 3 h at 37 °C.

    Techniques: Generated

    Nucleotide sequence of the 1,143-bp amplicon from the 23S rRNA gene from an H. pylori -resistant isolate. The arrow indicates a single point mutation, that is, a T2717C transition. The underline indicates the Hha I restriction site position. Arrows from the 5′ to 3′ termini indicate primer sequences.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: New Site of Modification of 23S rRNA Associated with Clarithromycin Resistance of Helicobacter pylori Clinical Isolates

    doi: 10.1128/AAC.46.12.3765-3769.2002

    Figure Lengend Snippet: Nucleotide sequence of the 1,143-bp amplicon from the 23S rRNA gene from an H. pylori -resistant isolate. The arrow indicates a single point mutation, that is, a T2717C transition. The underline indicates the Hha I restriction site position. Arrows from the 5′ to 3′ termini indicate primer sequences.

    Article Snippet: For rapid detection of the T2717C mutation, 5 U of Hha I (Amersham Pharmacia) was added to 10 μl of each amplicon and digestion was performed at 37°C for 2 h, as recommended by the manufacturer.

    Techniques: Sequencing, Amplification, Mutagenesis

    Hha I restriction analysis of 1,143-bp amplicons from 23S RNA gene. Lanes 1 to 7, the digestion products of seven Cla r isolates showing two fragments, respectively, of 440 and 517 bp. Lane HP ATCC, with two clearly distinguishable bands of 440 and 617 bp, corresponds to restriction analysis of the susceptible isolates. Lane MW, molecular weight marker 100-bp DNA ladder, whose fragment size has been reported on the left of the figure.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: New Site of Modification of 23S rRNA Associated with Clarithromycin Resistance of Helicobacter pylori Clinical Isolates

    doi: 10.1128/AAC.46.12.3765-3769.2002

    Figure Lengend Snippet: Hha I restriction analysis of 1,143-bp amplicons from 23S RNA gene. Lanes 1 to 7, the digestion products of seven Cla r isolates showing two fragments, respectively, of 440 and 517 bp. Lane HP ATCC, with two clearly distinguishable bands of 440 and 617 bp, corresponds to restriction analysis of the susceptible isolates. Lane MW, molecular weight marker 100-bp DNA ladder, whose fragment size has been reported on the left of the figure.

    Article Snippet: For rapid detection of the T2717C mutation, 5 U of Hha I (Amersham Pharmacia) was added to 10 μl of each amplicon and digestion was performed at 37°C for 2 h, as recommended by the manufacturer.

    Techniques: Molecular Weight, Marker

    DNA- and mRNA-based T-RFLP profiles of nifH homologs from the hindgut of four termite species. PCR products (∼470 bp) were digested with Hha I. Peak assignment is based on T-RF lengths of the nifH homologs obtained from the respective clone

    Journal: The ISME Journal

    Article Title: Bacteroidales ectosymbionts of gut flagellates shape the nitrogen-fixing community in dry-wood termites

    doi: 10.1038/ismej.2011.194

    Figure Lengend Snippet: DNA- and mRNA-based T-RFLP profiles of nifH homologs from the hindgut of four termite species. PCR products (∼470 bp) were digested with Hha I. Peak assignment is based on T-RF lengths of the nifH homologs obtained from the respective clone

    Article Snippet: Amplicons were digested with the restriction enzyme Hha I (Promega), and terminal restriction fragment (T-RF) length polymorphism (T-RFLP) was analyzed as described previously ( ).

    Techniques: Polymerase Chain Reaction

    Genotyping of the FABP2 (rs1799883) Ala54Thr polymorphism. After digestion of the polymerase chain reaction PCR product with Hha I in a polyacrylamide-gel electrophoresis stained with silver nitrate, P1 samples heterozygotes have three bands, at 180 bp, 99 bp and 81pb, P2 homozygotes have two bands at 99 bp and 81 bp. P3 homozygotes have one band at 180 bp.

    Journal: Journal of Cardiovascular Development and Disease

    Article Title: Postprandial Hypertriglyceridemia Is Associated with the Variant 54 Threonine FABP2 Gene

    doi: 10.3390/jcdd5030047

    Figure Lengend Snippet: Genotyping of the FABP2 (rs1799883) Ala54Thr polymorphism. After digestion of the polymerase chain reaction PCR product with Hha I in a polyacrylamide-gel electrophoresis stained with silver nitrate, P1 samples heterozygotes have three bands, at 180 bp, 99 bp and 81pb, P2 homozygotes have two bands at 99 bp and 81 bp. P3 homozygotes have one band at 180 bp.

    Article Snippet: The PCR product (180 bp) was digested with restriction enzyme Hha I (Invitrogen, Carlsbad, CA, USA).

    Techniques: Polymerase Chain Reaction, Polyacrylamide Gel Electrophoresis, Staining

    T-RFLP profile of the 16S rRNA genes of the actinobacterial population amplified from the roots of 6-week-old wheat grown in the field soil obtained from Swedes Flat and digested with Hin fI (a), Hha I (b), and Mbo I (c).

    Journal: Applied and Environmental Microbiology

    Article Title: Analysis of the Endophytic Actinobacterial Population in the Roots of Wheat (Triticum aestivum L.) by Terminal Restriction Fragment Length Polymorphism and Sequencing of 16S rRNA Clones

    doi: 10.1128/AEM.70.3.1787-1794.2004

    Figure Lengend Snippet: T-RFLP profile of the 16S rRNA genes of the actinobacterial population amplified from the roots of 6-week-old wheat grown in the field soil obtained from Swedes Flat and digested with Hin fI (a), Hha I (b), and Mbo I (c).

    Article Snippet: From the restriction patterns generated with Hha I, plasmids that showed different restriction fragment patterns were further digested with the restriction enzyme Hin fI (Promega) to further identify plasmids with dissimilar 16S rRNA RFLPs.

    Techniques: Amplification