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  • 99
    ATCC escherichia coli k12 hfr
    Escherichia Coli K12 Hfr, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore anti flag hfr coupled antibodies
    Anti Flag Hfr Coupled Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Zhejiang Tianyuan Bio hfrs inactivated vaccine youerjian
    Hfrs Inactivated Vaccine Youerjian, supplied by Zhejiang Tianyuan Bio, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hfrs  (Amazon)
    90
    Amazon hfrs
    Hfrs, supplied by Amazon, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher hfrs
    Comparison of IFN-γ-secreting CD8 + T cell frequency and activity after HTNV MAP and single-peptide stimulation. IFN-γ-secreting cell frequency was detected by enzyme-linked immunospot (ELISPOT) assay. CD4 + T cell-depleted PBMCs from HLA-A*02 + patients with <t>HFRS</t> were stimulated with single peptide (80 μmol/L), MAP (80 μmol/L) or phytohemagglutinin (10 μg/mL). HTNV single <t>CTL</t> epitope LIWTGMIDL, VMASLVWPV, SLTECPTFL, and FVVPILLKA are represented by LL9, VV9, SL9, and FA9, respectively. a The frequency of IFN-γ-secreting CD8 + T cells was detected. All cell frequencies were converted to the number of spot-forming cells per 1 × 10 6 CD4 + T cell-depleted PBMCs (SFCs/10 6 cells). b The activity of IFN-γ-secreting CD8 + T cells was measured. The activity was evaluated as the average of the spot size and intensity for each well. Each black dot represents one patient sample. The Mann-Whitney U test was used to determine the difference between two groups. The black line indicates the median and corresponding interquartile range (IQR). * P
    Hfrs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Zhejiang Tianyuan Bio hfrs inactivated vaccine
    Comparison of IFN-γ-secreting CD8 + T cell frequency and activity after HTNV MAP and single-peptide stimulation. IFN-γ-secreting cell frequency was detected by enzyme-linked immunospot (ELISPOT) assay. CD4 + T cell-depleted PBMCs from HLA-A*02 + patients with <t>HFRS</t> were stimulated with single peptide (80 μmol/L), MAP (80 μmol/L) or phytohemagglutinin (10 μg/mL). HTNV single <t>CTL</t> epitope LIWTGMIDL, VMASLVWPV, SLTECPTFL, and FVVPILLKA are represented by LL9, VV9, SL9, and FA9, respectively. a The frequency of IFN-γ-secreting CD8 + T cells was detected. All cell frequencies were converted to the number of spot-forming cells per 1 × 10 6 CD4 + T cell-depleted PBMCs (SFCs/10 6 cells). b The activity of IFN-γ-secreting CD8 + T cells was measured. The activity was evaluated as the average of the spot size and intensity for each well. Each black dot represents one patient sample. The Mann-Whitney U test was used to determine the difference between two groups. The black line indicates the median and corresponding interquartile range (IQR). * P
    Hfrs Inactivated Vaccine, supplied by Zhejiang Tianyuan Bio, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Green Cross Inc hfrs vaccination
    Comparison of IFN-γ-secreting CD8 + T cell frequency and activity after HTNV MAP and single-peptide stimulation. IFN-γ-secreting cell frequency was detected by enzyme-linked immunospot (ELISPOT) assay. CD4 + T cell-depleted PBMCs from HLA-A*02 + patients with <t>HFRS</t> were stimulated with single peptide (80 μmol/L), MAP (80 μmol/L) or phytohemagglutinin (10 μg/mL). HTNV single <t>CTL</t> epitope LIWTGMIDL, VMASLVWPV, SLTECPTFL, and FVVPILLKA are represented by LL9, VV9, SL9, and FA9, respectively. a The frequency of IFN-γ-secreting CD8 + T cells was detected. All cell frequencies were converted to the number of spot-forming cells per 1 × 10 6 CD4 + T cell-depleted PBMCs (SFCs/10 6 cells). b The activity of IFN-γ-secreting CD8 + T cells was measured. The activity was evaluated as the average of the spot size and intensity for each well. Each black dot represents one patient sample. The Mann-Whitney U test was used to determine the difference between two groups. The black line indicates the median and corresponding interquartile range (IQR). * P
    Hfrs Vaccination, supplied by Green Cross Inc, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche hfrs surveillance data
    Comparison of IFN-γ-secreting CD8 + T cell frequency and activity after HTNV MAP and single-peptide stimulation. IFN-γ-secreting cell frequency was detected by enzyme-linked immunospot (ELISPOT) assay. CD4 + T cell-depleted PBMCs from HLA-A*02 + patients with <t>HFRS</t> were stimulated with single peptide (80 μmol/L), MAP (80 μmol/L) or phytohemagglutinin (10 μg/mL). HTNV single <t>CTL</t> epitope LIWTGMIDL, VMASLVWPV, SLTECPTFL, and FVVPILLKA are represented by LL9, VV9, SL9, and FA9, respectively. a The frequency of IFN-γ-secreting CD8 + T cells was detected. All cell frequencies were converted to the number of spot-forming cells per 1 × 10 6 CD4 + T cell-depleted PBMCs (SFCs/10 6 cells). b The activity of IFN-γ-secreting CD8 + T cells was measured. The activity was evaluated as the average of the spot size and intensity for each well. Each black dot represents one patient sample. The Mann-Whitney U test was used to determine the difference between two groups. The black line indicates the median and corresponding interquartile range (IQR). * P
    Hfrs Surveillance Data, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Esri inc hfrs incidence
    Temporal distribution of monthly <t>HFRS</t> incidence in Shuangyang County and of the combined monthly incidence totals for the other nine counties in <t>Changchun.</t> The upper and lower panels represent the monthly incidence in Shuangyang County and the combined total monthly incidence for other nine counties, respectively. Red and black colors indicate monthly incidence for the February–July and August–January periods, respectively.
    Hfrs Incidence, supplied by Esri inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson hfr ad
    Temporal distribution of monthly <t>HFRS</t> incidence in Shuangyang County and of the combined monthly incidence totals for the other nine counties in <t>Changchun.</t> The upper and lower panels represent the monthly incidence in Shuangyang County and the combined total monthly incidence for other nine counties, respectively. Red and black colors indicate monthly incidence for the February–July and August–January periods, respectively.
    Hfr Ad, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    erbb4  (Abcam)
    88
    Abcam erbb4
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    Erbb4, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck & Co strain hfr
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    Strain Hfr, supplied by Merck & Co, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    research diets inc hf hfr group
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    Hf Hfr Group, supplied by research diets inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology hfr erbb4 antibody
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    Hfr Erbb4 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Abcam hfr 1 monoclonal antibody
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    Hfr 1 Monoclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Merck & Co escherichia coli strain hfr
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    Escherichia Coli Strain Hfr, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cassini meudon cassini rpws hfr polarization database
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    Meudon Cassini Rpws Hfr Polarization Database, supplied by Cassini, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC escherichia coli hfr k 12
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    Escherichia Coli Hfr K 12, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli hfr k12
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    E Coli Hfr K12, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    ATCC e coli k 12 hfr atcc 23631
    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) <t>ErbB4</t> in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.
    E Coli K 12 Hfr Atcc 23631, supplied by ATCC, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Nanoprobes rhodamine labeled expansile nanoparticles hfr enps
    Demonstration of the feasibility of utilizing <t>HFR-eNPs</t> for cytoreductive surgery in a xenograft Panc-1-CSC rat model. Shown are bright light and corresponding UV light images revealing the presence of fluorescent tumors before, but not after, resection. The peritoneal organs are shown in situ as well as ex vivo . Scale bars are 1 cm.
    Rhodamine Labeled Expansile Nanoparticles Hfr Enps, supplied by Nanoprobes, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Comparison of IFN-γ-secreting CD8 + T cell frequency and activity after HTNV MAP and single-peptide stimulation. IFN-γ-secreting cell frequency was detected by enzyme-linked immunospot (ELISPOT) assay. CD4 + T cell-depleted PBMCs from HLA-A*02 + patients with HFRS were stimulated with single peptide (80 μmol/L), MAP (80 μmol/L) or phytohemagglutinin (10 μg/mL). HTNV single CTL epitope LIWTGMIDL, VMASLVWPV, SLTECPTFL, and FVVPILLKA are represented by LL9, VV9, SL9, and FA9, respectively. a The frequency of IFN-γ-secreting CD8 + T cells was detected. All cell frequencies were converted to the number of spot-forming cells per 1 × 10 6 CD4 + T cell-depleted PBMCs (SFCs/10 6 cells). b The activity of IFN-γ-secreting CD8 + T cells was measured. The activity was evaluated as the average of the spot size and intensity for each well. Each black dot represents one patient sample. The Mann-Whitney U test was used to determine the difference between two groups. The black line indicates the median and corresponding interquartile range (IQR). * P

    Journal: Virology Journal

    Article Title: Design and synthesis of HLA-A*02-restricted Hantaan virus multiple-antigenic peptide for CD8+ T cells

    doi: 10.1186/s12985-020-1290-x

    Figure Lengend Snippet: Comparison of IFN-γ-secreting CD8 + T cell frequency and activity after HTNV MAP and single-peptide stimulation. IFN-γ-secreting cell frequency was detected by enzyme-linked immunospot (ELISPOT) assay. CD4 + T cell-depleted PBMCs from HLA-A*02 + patients with HFRS were stimulated with single peptide (80 μmol/L), MAP (80 μmol/L) or phytohemagglutinin (10 μg/mL). HTNV single CTL epitope LIWTGMIDL, VMASLVWPV, SLTECPTFL, and FVVPILLKA are represented by LL9, VV9, SL9, and FA9, respectively. a The frequency of IFN-γ-secreting CD8 + T cells was detected. All cell frequencies were converted to the number of spot-forming cells per 1 × 10 6 CD4 + T cell-depleted PBMCs (SFCs/10 6 cells). b The activity of IFN-γ-secreting CD8 + T cells was measured. The activity was evaluated as the average of the spot size and intensity for each well. Each black dot represents one patient sample. The Mann-Whitney U test was used to determine the difference between two groups. The black line indicates the median and corresponding interquartile range (IQR). * P

    Article Snippet: CFSE staining for proliferation assay To evaluate the capacity of specific CTL proliferation, 2 × 107 /mL PBMCs from HLA-A*02+ patients with HFRS were incubated with 10 μmol/L 6-carboxyfluorescein succinimidyl ester (CFSE, Molecular Probes, OR) for 15 min at 37 °C and kept away from light.

    Techniques: Activity Assay, Enzyme-linked Immunospot, MANN-WHITNEY

    Representative CFSE staining of CD8 + T cell proliferation stimulated with HTNV MAP and single peptide. Isolated PBMCs from HLA-A*02 + patients with HFRS were labeled with CFSE (5 μM) and stimulated with HTNV MAP or single epitope LL9, VV9, SL9 and FA9. The cells were stained with anti-CD3 PerCP-Cy5.5 and anti-CD8 PE mAb after 5 days of culture. a The scatter plot for the flow cytometry analysis of cell proliferation from one representative HLA-A*02 + HFRS patient after stimulation with different groups of stimuli when gated on CD3 + cells. The upper left quadrant shows that the CFSE fluorescence intensity was reduced in cells representing the proliferation percentages. The number indicates the percentage of cells in the boxed area. b The histogram shows the peak shift of the CFSE dim CD3 + CD8 + T cells in different stimulation groups. The degree of shift to the left of the curve reflects the proliferation percentage of CD8 + T cells. The SEB stimulation group was used as a positive control, and the no stimulation group was used as a negative control

    Journal: Virology Journal

    Article Title: Design and synthesis of HLA-A*02-restricted Hantaan virus multiple-antigenic peptide for CD8+ T cells

    doi: 10.1186/s12985-020-1290-x

    Figure Lengend Snippet: Representative CFSE staining of CD8 + T cell proliferation stimulated with HTNV MAP and single peptide. Isolated PBMCs from HLA-A*02 + patients with HFRS were labeled with CFSE (5 μM) and stimulated with HTNV MAP or single epitope LL9, VV9, SL9 and FA9. The cells were stained with anti-CD3 PerCP-Cy5.5 and anti-CD8 PE mAb after 5 days of culture. a The scatter plot for the flow cytometry analysis of cell proliferation from one representative HLA-A*02 + HFRS patient after stimulation with different groups of stimuli when gated on CD3 + cells. The upper left quadrant shows that the CFSE fluorescence intensity was reduced in cells representing the proliferation percentages. The number indicates the percentage of cells in the boxed area. b The histogram shows the peak shift of the CFSE dim CD3 + CD8 + T cells in different stimulation groups. The degree of shift to the left of the curve reflects the proliferation percentage of CD8 + T cells. The SEB stimulation group was used as a positive control, and the no stimulation group was used as a negative control

    Article Snippet: CFSE staining for proliferation assay To evaluate the capacity of specific CTL proliferation, 2 × 107 /mL PBMCs from HLA-A*02+ patients with HFRS were incubated with 10 μmol/L 6-carboxyfluorescein succinimidyl ester (CFSE, Molecular Probes, OR) for 15 min at 37 °C and kept away from light.

    Techniques: Staining, Isolation, Labeling, Flow Cytometry, Fluorescence, Positive Control, Negative Control

    Temporal distribution of monthly HFRS incidence in Shuangyang County and of the combined monthly incidence totals for the other nine counties in Changchun. The upper and lower panels represent the monthly incidence in Shuangyang County and the combined total monthly incidence for other nine counties, respectively. Red and black colors indicate monthly incidence for the February–July and August–January periods, respectively.

    Journal: BMC Infectious Diseases

    Article Title: Increasing incidence of hemorrhagic fever with renal syndrome could be associated with livestock husbandry in Changchun, Northeastern China

    doi: 10.1186/1471-2334-14-301

    Figure Lengend Snippet: Temporal distribution of monthly HFRS incidence in Shuangyang County and of the combined monthly incidence totals for the other nine counties in Changchun. The upper and lower panels represent the monthly incidence in Shuangyang County and the combined total monthly incidence for other nine counties, respectively. Red and black colors indicate monthly incidence for the February–July and August–January periods, respectively.

    Article Snippet: Analysis of epidemiologic patterns of human HFRS cases The thematic maps of annual incidence before and since 1998 were created in gradient colors to display the spatial distribution of HFRS incidence in each county of Changchun (ArcGIS 9.3 software, ESRI Inc., Redlands, CA, USA).

    Techniques:

    Thematic map of annual incidence for each county before and since 1998, Changchun. The gradient colors represent HFRS incidence for each county, and the pie-charts with red and black colors indicate the proportions of HFRS cases for two 6-month study periods (beginning of the year (February–July) and end of the year (August–January)).

    Journal: BMC Infectious Diseases

    Article Title: Increasing incidence of hemorrhagic fever with renal syndrome could be associated with livestock husbandry in Changchun, Northeastern China

    doi: 10.1186/1471-2334-14-301

    Figure Lengend Snippet: Thematic map of annual incidence for each county before and since 1998, Changchun. The gradient colors represent HFRS incidence for each county, and the pie-charts with red and black colors indicate the proportions of HFRS cases for two 6-month study periods (beginning of the year (February–July) and end of the year (August–January)).

    Article Snippet: Analysis of epidemiologic patterns of human HFRS cases The thematic maps of annual incidence before and since 1998 were created in gradient colors to display the spatial distribution of HFRS incidence in each county of Changchun (ArcGIS 9.3 software, ESRI Inc., Redlands, CA, USA).

    Techniques:

    Age, gender, and occupational distribution of reported HFRS cases. (A) Average incidence over age groups and sex during two epidemiological phases (1988–1997 and 1998–2012). (B) Occupational proportions for HFRS cases during the two phases. Peasants indicate people engaged in farming or livestock breeding; workers indicate people who work in manufacturing; students are grade school pupils, high school students, and undergraduates; public servants are teachers, doctors, civil servants, and individuals retired from these occupations; migrant laborers are migrant workers, restaurant servers, shop workers and housekeepers; preschool child indicates children

    Journal: BMC Infectious Diseases

    Article Title: Increasing incidence of hemorrhagic fever with renal syndrome could be associated with livestock husbandry in Changchun, Northeastern China

    doi: 10.1186/1471-2334-14-301

    Figure Lengend Snippet: Age, gender, and occupational distribution of reported HFRS cases. (A) Average incidence over age groups and sex during two epidemiological phases (1988–1997 and 1998–2012). (B) Occupational proportions for HFRS cases during the two phases. Peasants indicate people engaged in farming or livestock breeding; workers indicate people who work in manufacturing; students are grade school pupils, high school students, and undergraduates; public servants are teachers, doctors, civil servants, and individuals retired from these occupations; migrant laborers are migrant workers, restaurant servers, shop workers and housekeepers; preschool child indicates children

    Article Snippet: Analysis of epidemiologic patterns of human HFRS cases The thematic maps of annual incidence before and since 1998 were created in gradient colors to display the spatial distribution of HFRS incidence in each county of Changchun (ArcGIS 9.3 software, ESRI Inc., Redlands, CA, USA).

    Techniques:

    Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) ErbB4 in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.

    Journal: American Journal of Cancer Research

    Article Title: Biomarkers and endosalpingiosis in the ovarian and tubal microenvironment of women at high-risk for pelvic serous carcinoma

    doi:

    Figure Lengend Snippet: Boxplots of immunohistochemical scores for (A) CSF-1 in ovarian epithelium and (B) ErbB4 in ovarian stroma, by HR status. Median (line) with 25 th and 75 th percentiles are depicted by the box. ◊indicates the mean.

    Article Snippet: The antibodies for ErbB4 (ab19391, Abcam), CSF-1R (ab61137, Abcam) and its ligand, CSF-1 (ab9693, Abcam), were utilized.

    Techniques: Immunohistochemistry

    Receiver Operating Curve for the prediction of being HR, based on a model of CSF-1 in ovarian epithelium, ErbB4 in ovarian stroma, and age. This model achieves a C value of 0.87, with 73% sensitivity and 93% specificity.

    Journal: American Journal of Cancer Research

    Article Title: Biomarkers and endosalpingiosis in the ovarian and tubal microenvironment of women at high-risk for pelvic serous carcinoma

    doi:

    Figure Lengend Snippet: Receiver Operating Curve for the prediction of being HR, based on a model of CSF-1 in ovarian epithelium, ErbB4 in ovarian stroma, and age. This model achieves a C value of 0.87, with 73% sensitivity and 93% specificity.

    Article Snippet: The antibodies for ErbB4 (ab19391, Abcam), CSF-1R (ab61137, Abcam) and its ligand, CSF-1 (ab9693, Abcam), were utilized.

    Techniques:

    Representative examples of (A) CSF-1 or (B) ErbB4 expression in ovarian or tubal epithelium and stroma, by immunohistochemistry. Scores for the staining are in parentheses. An example of ErbB4 nuclear staining is also shown in ovarian endosalpingiosis.

    Journal: American Journal of Cancer Research

    Article Title: Biomarkers and endosalpingiosis in the ovarian and tubal microenvironment of women at high-risk for pelvic serous carcinoma

    doi:

    Figure Lengend Snippet: Representative examples of (A) CSF-1 or (B) ErbB4 expression in ovarian or tubal epithelium and stroma, by immunohistochemistry. Scores for the staining are in parentheses. An example of ErbB4 nuclear staining is also shown in ovarian endosalpingiosis.

    Article Snippet: The antibodies for ErbB4 (ab19391, Abcam), CSF-1R (ab61137, Abcam) and its ligand, CSF-1 (ab9693, Abcam), were utilized.

    Techniques: Expressing, Immunohistochemistry, Staining

    Demonstration of the feasibility of utilizing HFR-eNPs for cytoreductive surgery in a xenograft Panc-1-CSC rat model. Shown are bright light and corresponding UV light images revealing the presence of fluorescent tumors before, but not after, resection. The peritoneal organs are shown in situ as well as ex vivo . Scale bars are 1 cm.

    Journal: ACS nano

    Article Title: Highly Specific and Sensitive Fluorescent Nanoprobes for Image-Guided Resection of Sub-Millimeter Peritoneal Tumors

    doi: 10.1021/acsnano.6b06777

    Figure Lengend Snippet: Demonstration of the feasibility of utilizing HFR-eNPs for cytoreductive surgery in a xenograft Panc-1-CSC rat model. Shown are bright light and corresponding UV light images revealing the presence of fluorescent tumors before, but not after, resection. The peritoneal organs are shown in situ as well as ex vivo . Scale bars are 1 cm.

    Article Snippet: Building on our observation that expansile nanoparticles (eNPs) localize to peritoneal tumors after intraperitoneal (IP) injection, – we are developing highly fluorescent, rhodamine-labeled expansile nanoparticles (HFR-eNPs) as nanoprobes to assist in the visualization and resection of intraperitoneal tumors that are not detectable via current clinical imaging modalities ( i.e. , sub-centimeter tumors) or by the surgeon intraoperatively ( i.e. , sub-millimeter tumors) in three different human tumor models of intraperitoneal carcinomatosis of mesothelial, ovarian, and pancreatic origin.

    Techniques: In Situ, Ex Vivo

    Highly fluorescent rhodamine-labeled expansile nanoparticles (HFR-eNPs, yellow circles in UV light images) localize to intraperitoneal tumors (white circles in bright light images) in models of pancreatic, mesothelioma, and ovarian carcinomatoses. All scale bars are 1 cm.

    Journal: ACS nano

    Article Title: Highly Specific and Sensitive Fluorescent Nanoprobes for Image-Guided Resection of Sub-Millimeter Peritoneal Tumors

    doi: 10.1021/acsnano.6b06777

    Figure Lengend Snippet: Highly fluorescent rhodamine-labeled expansile nanoparticles (HFR-eNPs, yellow circles in UV light images) localize to intraperitoneal tumors (white circles in bright light images) in models of pancreatic, mesothelioma, and ovarian carcinomatoses. All scale bars are 1 cm.

    Article Snippet: Building on our observation that expansile nanoparticles (eNPs) localize to peritoneal tumors after intraperitoneal (IP) injection, – we are developing highly fluorescent, rhodamine-labeled expansile nanoparticles (HFR-eNPs) as nanoprobes to assist in the visualization and resection of intraperitoneal tumors that are not detectable via current clinical imaging modalities ( i.e. , sub-centimeter tumors) or by the surgeon intraoperatively ( i.e. , sub-millimeter tumors) in three different human tumor models of intraperitoneal carcinomatosis of mesothelial, ovarian, and pancreatic origin.

    Techniques: Labeling

    Comparison between three different fluorescent probes, including HFR-eNPs, Rho-PLGA-NPs, and HFR-PEG-eNPs. The Rho-PLGA-NPs have a similar effective charge but lack pH-responsive functionality, whereas the HFR-PEG-eNPs maintain the pH-responsive functionality but have a more neutral effective charge. Only the HFR-eNPs show tumor localization in vivo. White circles in bright light images mark regions of tumor; yellow circles in UV light images highlight regions of NP fluorescence. All scale bars are 1 cm.

    Journal: ACS nano

    Article Title: Highly Specific and Sensitive Fluorescent Nanoprobes for Image-Guided Resection of Sub-Millimeter Peritoneal Tumors

    doi: 10.1021/acsnano.6b06777

    Figure Lengend Snippet: Comparison between three different fluorescent probes, including HFR-eNPs, Rho-PLGA-NPs, and HFR-PEG-eNPs. The Rho-PLGA-NPs have a similar effective charge but lack pH-responsive functionality, whereas the HFR-PEG-eNPs maintain the pH-responsive functionality but have a more neutral effective charge. Only the HFR-eNPs show tumor localization in vivo. White circles in bright light images mark regions of tumor; yellow circles in UV light images highlight regions of NP fluorescence. All scale bars are 1 cm.

    Article Snippet: Building on our observation that expansile nanoparticles (eNPs) localize to peritoneal tumors after intraperitoneal (IP) injection, – we are developing highly fluorescent, rhodamine-labeled expansile nanoparticles (HFR-eNPs) as nanoprobes to assist in the visualization and resection of intraperitoneal tumors that are not detectable via current clinical imaging modalities ( i.e. , sub-centimeter tumors) or by the surgeon intraoperatively ( i.e. , sub-millimeter tumors) in three different human tumor models of intraperitoneal carcinomatosis of mesothelial, ovarian, and pancreatic origin.

    Techniques: In Vivo, Fluorescence

    Visual assessment of HFR-eNP biodistribution. Representative bright light (BL) and long-wave UV light images of both whole and sectioned major organs 3 days following IP injection of 0.2% HFR-eNPs. Shown are the heart, collapsed lungs, brain, liver, spleen and pancreas, stomach with large and small intestines, mesothelium, kidneys with uterine horns, ovaries, bladder, adrenal glands, and adipose tissue. The sectioned stomach and intestine are shown with and without contents. Whole and sectioned UV images confirm nonfluorescence in these organs. All scale bars are 1 cm.

    Journal: ACS nano

    Article Title: Highly Specific and Sensitive Fluorescent Nanoprobes for Image-Guided Resection of Sub-Millimeter Peritoneal Tumors

    doi: 10.1021/acsnano.6b06777

    Figure Lengend Snippet: Visual assessment of HFR-eNP biodistribution. Representative bright light (BL) and long-wave UV light images of both whole and sectioned major organs 3 days following IP injection of 0.2% HFR-eNPs. Shown are the heart, collapsed lungs, brain, liver, spleen and pancreas, stomach with large and small intestines, mesothelium, kidneys with uterine horns, ovaries, bladder, adrenal glands, and adipose tissue. The sectioned stomach and intestine are shown with and without contents. Whole and sectioned UV images confirm nonfluorescence in these organs. All scale bars are 1 cm.

    Article Snippet: Building on our observation that expansile nanoparticles (eNPs) localize to peritoneal tumors after intraperitoneal (IP) injection, – we are developing highly fluorescent, rhodamine-labeled expansile nanoparticles (HFR-eNPs) as nanoprobes to assist in the visualization and resection of intraperitoneal tumors that are not detectable via current clinical imaging modalities ( i.e. , sub-centimeter tumors) or by the surgeon intraoperatively ( i.e. , sub-millimeter tumors) in three different human tumor models of intraperitoneal carcinomatosis of mesothelial, ovarian, and pancreatic origin.

    Techniques: Injection

    Analysis of HFR-eNP localization in Panc1-CSC tumor cells. (a) HFR-eNP localization excludes blood vessels and endothelium (arrows showing blood vessel cross sections); scale bars are 40 μ m. (b) Cross section of blood vessel wall showing no colocalization of HFR-eNPs; MT = Mason’s Trichrome; scale bars are 20 μ m. (c) Colocalization of HRF-eNPs and HIF1 α , a marker for hypoxia; scale bars are 40 μ m. (d) Colocalization of HFR-eNPs and LDH-A, a marker for tumor acidification; scale bars are 40 μ m.

    Journal: ACS nano

    Article Title: Highly Specific and Sensitive Fluorescent Nanoprobes for Image-Guided Resection of Sub-Millimeter Peritoneal Tumors

    doi: 10.1021/acsnano.6b06777

    Figure Lengend Snippet: Analysis of HFR-eNP localization in Panc1-CSC tumor cells. (a) HFR-eNP localization excludes blood vessels and endothelium (arrows showing blood vessel cross sections); scale bars are 40 μ m. (b) Cross section of blood vessel wall showing no colocalization of HFR-eNPs; MT = Mason’s Trichrome; scale bars are 20 μ m. (c) Colocalization of HRF-eNPs and HIF1 α , a marker for hypoxia; scale bars are 40 μ m. (d) Colocalization of HFR-eNPs and LDH-A, a marker for tumor acidification; scale bars are 40 μ m.

    Article Snippet: Building on our observation that expansile nanoparticles (eNPs) localize to peritoneal tumors after intraperitoneal (IP) injection, – we are developing highly fluorescent, rhodamine-labeled expansile nanoparticles (HFR-eNPs) as nanoprobes to assist in the visualization and resection of intraperitoneal tumors that are not detectable via current clinical imaging modalities ( i.e. , sub-centimeter tumors) or by the surgeon intraoperatively ( i.e. , sub-millimeter tumors) in three different human tumor models of intraperitoneal carcinomatosis of mesothelial, ovarian, and pancreatic origin.

    Techniques: Marker

    Characterization of HFR-eNPs. (a) Nanoparticle diameter as a function of rhodamine incorporation as measured by SEM and DLS. (b) SEM image of 0.2% HFR-eNPs; white arrows indicate two larger particles on a background of many 20–50 nm particles. (c) Representative DLS data of 0.2% HFR-eNPs. (d) Viability of Panc-1 cells treated with HFR-eNPs as measured using an MTS assay. (e) Area under the curve (AUC) of the fluorescence emission spectra of HFR-eNPs, Rho-PLGA-NPs, and free rhodamine B as a function of rhodamine concentration in 10 mM pH 7.4 phosphate buffer. Inset: Fluorescence emission spectra of the HFR-eNPs at equivalent polymer concentrations. (f) AUC of the fluorescence emission spectra of HFR-eNPs and Rho-PLGA-NPs as a function of polymer concentration in 10 mM pH 7.4 phosphate buffer.

    Journal: ACS nano

    Article Title: Highly Specific and Sensitive Fluorescent Nanoprobes for Image-Guided Resection of Sub-Millimeter Peritoneal Tumors

    doi: 10.1021/acsnano.6b06777

    Figure Lengend Snippet: Characterization of HFR-eNPs. (a) Nanoparticle diameter as a function of rhodamine incorporation as measured by SEM and DLS. (b) SEM image of 0.2% HFR-eNPs; white arrows indicate two larger particles on a background of many 20–50 nm particles. (c) Representative DLS data of 0.2% HFR-eNPs. (d) Viability of Panc-1 cells treated with HFR-eNPs as measured using an MTS assay. (e) Area under the curve (AUC) of the fluorescence emission spectra of HFR-eNPs, Rho-PLGA-NPs, and free rhodamine B as a function of rhodamine concentration in 10 mM pH 7.4 phosphate buffer. Inset: Fluorescence emission spectra of the HFR-eNPs at equivalent polymer concentrations. (f) AUC of the fluorescence emission spectra of HFR-eNPs and Rho-PLGA-NPs as a function of polymer concentration in 10 mM pH 7.4 phosphate buffer.

    Article Snippet: Building on our observation that expansile nanoparticles (eNPs) localize to peritoneal tumors after intraperitoneal (IP) injection, – we are developing highly fluorescent, rhodamine-labeled expansile nanoparticles (HFR-eNPs) as nanoprobes to assist in the visualization and resection of intraperitoneal tumors that are not detectable via current clinical imaging modalities ( i.e. , sub-centimeter tumors) or by the surgeon intraoperatively ( i.e. , sub-millimeter tumors) in three different human tumor models of intraperitoneal carcinomatosis of mesothelial, ovarian, and pancreatic origin.

    Techniques: MTS Assay, Fluorescence, Concentration Assay